KR100561122B1 - 서열화를 위해 변형시킨 열안정성 디엔에이 폴리머라제 - Google Patents

서열화를 위해 변형시킨 열안정성 디엔에이 폴리머라제 Download PDF

Info

Publication number
KR100561122B1
KR100561122B1 KR1019980037299A KR19980037299A KR100561122B1 KR 100561122 B1 KR100561122 B1 KR 100561122B1 KR 1019980037299 A KR1019980037299 A KR 1019980037299A KR 19980037299 A KR19980037299 A KR 19980037299A KR 100561122 B1 KR100561122 B1 KR 100561122B1
Authority
KR
South Korea
Prior art keywords
amino acid
xaa
labeled
polymerase
dna polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
KR1019980037299A
Other languages
English (en)
Korean (ko)
Other versions
KR19990029676A (ko
Inventor
데이비드 에이치 겔판드
리사 비비안 칼만
토마스 더블유 마이어스
프레드 로렌스 렐케르트
크리스토퍼 림 시구아
Original Assignee
에프. 호프만-라 로슈 아게
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에프. 호프만-라 로슈 아게 filed Critical 에프. 호프만-라 로슈 아게
Publication of KR19990029676A publication Critical patent/KR19990029676A/ko
Application granted granted Critical
Publication of KR100561122B1 publication Critical patent/KR100561122B1/ko
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1019980037299A 1997-09-11 1998-09-10 서열화를 위해 변형시킨 열안정성 디엔에이 폴리머라제 Expired - Fee Related KR100561122B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5852597P 1997-09-11 1997-09-11
US60/058,525 1997-09-11

Publications (2)

Publication Number Publication Date
KR19990029676A KR19990029676A (ko) 1999-04-26
KR100561122B1 true KR100561122B1 (ko) 2006-07-25

Family

ID=22017355

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019980037299A Expired - Fee Related KR100561122B1 (ko) 1997-09-11 1998-09-10 서열화를 위해 변형시킨 열안정성 디엔에이 폴리머라제

Country Status (17)

Country Link
US (3) US6346379B1 (enExample)
EP (1) EP0902035B1 (enExample)
JP (1) JP4417452B2 (enExample)
KR (1) KR100561122B1 (enExample)
CN (1) CN1197962C (enExample)
AT (1) ATE530567T1 (enExample)
AU (1) AU741366B2 (enExample)
BR (1) BR9803419B1 (enExample)
CA (1) CA2243985C (enExample)
CZ (1) CZ293217B6 (enExample)
ES (1) ES2374910T3 (enExample)
HU (1) HUP9802024A3 (enExample)
IL (1) IL126100A (enExample)
NO (1) NO327476B1 (enExample)
PL (1) PL328525A1 (enExample)
RU (1) RU2238978C2 (enExample)
TW (1) TW513439B (enExample)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2264045B1 (en) * 1996-08-14 2015-10-21 Life Technologies Corporation Stable compositions for nucleic acid amplification and sequencing
US7875440B2 (en) * 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US20030138834A1 (en) * 1998-08-19 2003-07-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US20040121394A1 (en) * 1998-08-19 2004-06-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1210440B1 (en) * 1999-08-21 2005-07-20 Amersham Biosciences Corp. Taq dna polymerases having an amino acid substitution at e681 and homologs thereof exhibiting improved salt tolerance
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
EP1368497A4 (en) 2001-03-12 2007-08-15 California Inst Of Techn METHODS AND APPARATUS FOR ANALYZING ASYNCHRONOUS BASE EXTENSION POLYNUCLEOTIDE SEQUENCES
DK1370690T3 (da) * 2001-03-16 2012-07-09 Kalim Mir Arrays og fremgangsmåder til anvendelse heraf
AU2002316586A1 (en) 2001-07-06 2003-01-21 Amersham Biosciences Corp Novel dna polymerases having amino acid substitutions and homologs thereof
WO2003068991A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
KR100571808B1 (ko) * 2003-04-16 2006-04-17 삼성전자주식회사 다층 박막 구조를 가진 ｄｎａ 칩
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
EP1716254B1 (en) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Methods for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
DE602005027700D1 (de) * 2004-05-25 2011-06-09 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7524831B2 (en) * 2005-03-02 2009-04-28 Schering Corporation Treatments for Flaviviridae virus infection
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
GB0518867D0 (en) * 2005-09-15 2005-10-26 Secretary Trade Ind Brit Microscopy tip
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
AU2006331677A1 (en) * 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Protein engineering strategies to optimize activity of surface attached proteins
ATE518010T1 (de) * 2005-12-22 2011-08-15 Pacific Biosciences California Aktive oberflächengekoppelte polymerasen
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
AU2007309504B2 (en) * 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20100081927A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081915A1 (en) * 2008-09-29 2010-04-01 Searete Llc, Alimited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081926A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081928A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological Facilitation systems and methods
US20100081916A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware. Histological facilitation systems and methods
US20100081924A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
FR2940291B1 (fr) 2008-12-23 2012-12-21 Isp Investments Inc Peptide derive d'hmg-coa reductase et composition cosmetique ou pharmaceutique le contenant
FR2940125B1 (fr) * 2008-12-23 2013-03-22 Isp Investments Inc Composition cosmetique ou pharmaceutique apaisante comprenant un peptide activateur de la hmg-coa reductase
US8933036B2 (en) 2009-04-15 2015-01-13 Isp Investments Inc. Cosmetic and/or pharmaceutical composition comprising a yeast peptide hydrolysate and use of the yeast peptide hydrolysate as an active agent for strengthening hair
FR2944445B1 (fr) 2009-04-15 2013-08-16 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique apaisant
FR2944526B1 (fr) 2009-04-15 2013-05-10 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere
EP2582808B1 (en) 2010-06-18 2015-04-22 Roche Diagniostics GmbH Dna polymerases with increased 3'-mismatch discrimination
EP2582804B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
JP5926247B2 (ja) 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
EP2582806B1 (en) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
JP5876477B2 (ja) 2010-06-18 2016-03-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
US8715991B2 (en) 2010-06-18 2014-05-06 Roche Molecular Systems, Inc. DNA polymerases with increased 3'-mismatch discrimination
US8722379B2 (en) 2010-06-18 2014-05-13 Roche Medical Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
KR101622442B1 (ko) 2010-06-18 2016-05-19 에프. 호프만-라 로슈 아게 ３’―미스매치 구별이 증가된 ｄｎａ 중합효소
US8765435B2 (en) 2011-02-15 2014-07-01 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
US9017979B2 (en) 2011-04-11 2015-04-28 Roche Molecular Systems, Inc. DNA polymerases with improved activity
US9090883B2 (en) 2011-07-28 2015-07-28 Roche Molecular Systems, Inc. DNA polymerases with improved activity
JP6224613B2 (ja) 2011-12-08 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
CA2858264C (en) 2011-12-08 2018-01-02 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
JP6088710B2 (ja) 2013-08-19 2017-03-01 シンギュラー・バイオ・インコーポレイテッド 単分子検出アッセイおよびその使用
CN108064278B (zh) 2014-11-14 2022-02-08 亿明达股份有限公司 聚合酶
WO2016124543A1 (en) 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants
WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
US10526588B2 (en) * 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
WO2017090684A1 (ja) 2015-11-27 2017-06-01 国立大学法人九州大学 Ｄｎａポリメラーゼ変異体
CN112703248B (zh) 2018-09-13 2025-03-25 豪夫迈·罗氏有限公司 具有改良链置换能力的突变型dna聚合酶
US20240287481A1 (en) * 2021-03-19 2024-08-29 Mgi Tech Co., Ltd. Polymerases for efficient incorporation of nucleotides with 3'-phosphate and other 3'-terminators

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
CA2018273C (en) 1989-06-09 1999-04-06 Peter D. Senter Thermally stable cytosine deaminase
US5338678A (en) 1989-06-09 1994-08-16 Oncogen, A Limited Partnership Expression of DNA sequences encoding a thermally stable cytosine deaminase from saccharomyces
US5292658A (en) * 1989-12-29 1994-03-08 University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center Cloning and expressions of Renilla luciferase
US6083686A (en) * 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5446591A (en) * 1993-02-08 1995-08-29 Lockheed Missiles & Space Co., Inc. Lens mounting for use with liquid lens elements
US5571706A (en) * 1994-06-17 1996-11-05 The United States Of America As Represented By The Secretary Of Agriculture Plant virus resistance gene and methods
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
EP0655506B1 (en) * 1994-10-17 1996-09-18 President And Fellows Of Harvard College DNA polymerases having modified nucleotide binding site for DNA sequencing
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5852191A (en) * 1995-06-07 1998-12-22 Carnegie Mellon University Rigidized monomethine cyanines
US6107029A (en) * 1996-07-31 2000-08-22 Message Pharmaceticals, Inc. Universal method for detecting interactions between RNA molecules and RNA binding proteins
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
US5723298A (en) * 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
AU743025B2 (en) * 1997-03-12 2002-01-17 Applera Corporation DNA polymerases having improved labeled nucleotide incorporation properties

Also Published As

Publication number Publication date
PL328525A1 (en) 1999-03-15
EP0902035A2 (en) 1999-03-17
IL126100A (en) 2005-12-18
US6346379B1 (en) 2002-02-12
US7598032B2 (en) 2009-10-06
HU9802024D0 (en) 1998-12-28
BR9803419A (pt) 2000-02-08
CA2243985C (en) 2004-08-10
CA2243985A1 (en) 1999-03-11
EP0902035B1 (en) 2011-10-26
TW513439B (en) 2002-12-11
JPH11137284A (ja) 1999-05-25
HUP9802024A3 (en) 2001-02-28
US20030152988A1 (en) 2003-08-14
NO327476B1 (no) 2009-07-13
BR9803419B1 (pt) 2010-09-21
ATE530567T1 (de) 2011-11-15
KR19990029676A (ko) 1999-04-26
EP0902035A3 (en) 1999-06-23
CZ287498A3 (cs) 1999-03-17
NO984157D0 (no) 1998-09-10
HUP9802024A2 (hu) 1999-05-28
JP4417452B2 (ja) 2010-02-17
CZ293217B6 (cs) 2004-03-17
AU741366B2 (en) 2001-11-29
RU2238978C2 (ru) 2004-10-27
CN1218832A (zh) 1999-06-09
NO984157L (no) 1999-03-12
US20020142333A1 (en) 2002-10-03
ES2374910T3 (es) 2012-02-23
IL126100A0 (en) 1999-05-09
AU8416198A (en) 1999-03-25
CN1197962C (zh) 2005-04-20
HK1018796A1 (en) 2000-01-07

Similar Documents

Publication Publication Date Title
KR100561122B1 (ko) 서열화를 위해 변형시킨 열안정성 디엔에이 폴리머라제
US5939292A (en) Thermostable DNA polymerases having reduced discrimination against ribo-NTPs
US5614365A (en) DNA polymerase having modified nucleotide binding site for DNA sequencing
EP0655506B1 (en) DNA polymerases having modified nucleotide binding site for DNA sequencing
US5001050A (en) PHφ29 DNA polymerase
US5576204A (en) φ29 DNA polymerase
CA2222744C (en) Thermostable dna polymerases
US5405774A (en) DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
WO1996012042A9 (en) Dna polymerases having modified nucleotide binding site for dna sequencing
JP2003526378A (ja) 高忠実度逆転写酵素およびその使用
JP3112148B2 (ja) 核酸の増幅方法およびその試薬
US5834253A (en) Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity
Blanco et al. In vitro DNA synthesis reactions using phi29 DNA polymerase and a DNA fragment encoding said polymerase
JPH07123986A (ja) Ｄｎａポリメラーゼ遺伝子
JP2000354496A (ja) 核酸の増幅方法およびその試薬
JP2000023687A (ja) 熱安定性ｄｎａポリメラ―ゼ

Legal Events

Date Code Title Description
PA0109 Patent application

St.27 status event code: A-0-1-A10-A12-nap-PA0109

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

A201 Request for examination
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U11-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

FPAY Annual fee payment

Payment date: 20120228

Year of fee payment: 7

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

FPAY Annual fee payment

Payment date: 20130227

Year of fee payment: 8

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 8

LAPS Lapse due to unpaid annual fee
PC1903 Unpaid annual fee

St.27 status event code: A-4-4-U10-U13-oth-PC1903

Not in force date: 20140309

Payment event data comment text: Termination Category : DEFAULT_OF_REGISTRATION_FEE

PC1903 Unpaid annual fee

St.27 status event code: N-4-6-H10-H13-oth-PC1903

Ip right cessation event data comment text: Termination Category : DEFAULT_OF_REGISTRATION_FEE

Not in force date: 20140309

P22-X000 Classification modified

St.27 status event code: A-4-4-P10-P22-nap-X000

R18-X000 Changes to party contact information recorded

St.27 status event code: A-5-5-R10-R18-oth-X000