BR9803419B1 - polimerase de dna termoestável recombinante e processo para produção e uso da mesma, seqüência de ácido nucléico, vetor, célula hospedeira, processo para seqüenciamento de dna, para produção de dna marcado e conjunto para seqüenciamento de dna e para produzir produtos de extensão de primer marcados. - Google Patents

polimerase de dna termoestável recombinante e processo para produção e uso da mesma, seqüência de ácido nucléico, vetor, célula hospedeira, processo para seqüenciamento de dna, para produção de dna marcado e conjunto para seqüenciamento de dna e para produzir produtos de extensão de primer marcados.

Info

Publication number
BR9803419B1
BR9803419B1 BRPI9803419-7A BR9803419A BR9803419B1 BR 9803419 B1 BR9803419 B1 BR 9803419B1 BR 9803419 A BR9803419 A BR 9803419A BR 9803419 B1 BR9803419 B1 BR 9803419B1
Authority
BR
Brazil
Prior art keywords
dna
production
dna sequencing
tagged
sequencing
Prior art date
Application number
BRPI9803419-7A
Other languages
English (en)
Portuguese (pt)
Other versions
BR9803419A (pt
Inventor
David H Gelfand
Lisa Vivian Kalman
Thomas W Myers
Fred Lawrence Reichert
Christopher Lim Sigua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of BR9803419A publication Critical patent/BR9803419A/pt
Publication of BR9803419B1 publication Critical patent/BR9803419B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI9803419-7A 1997-09-11 1998-09-10 polimerase de dna termoestável recombinante e processo para produção e uso da mesma, seqüência de ácido nucléico, vetor, célula hospedeira, processo para seqüenciamento de dna, para produção de dna marcado e conjunto para seqüenciamento de dna e para produzir produtos de extensão de primer marcados. BR9803419B1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5852597P 1997-09-11 1997-09-11

Publications (2)

Publication Number Publication Date
BR9803419A BR9803419A (pt) 2000-02-08
BR9803419B1 true BR9803419B1 (pt) 2010-09-21

Family

ID=22017355

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9803419-7A BR9803419B1 (pt) 1997-09-11 1998-09-10 polimerase de dna termoestável recombinante e processo para produção e uso da mesma, seqüência de ácido nucléico, vetor, célula hospedeira, processo para seqüenciamento de dna, para produção de dna marcado e conjunto para seqüenciamento de dna e para produzir produtos de extensão de primer marcados.

Country Status (17)

Country Link
US (3) US6346379B1 (enExample)
EP (1) EP0902035B1 (enExample)
JP (1) JP4417452B2 (enExample)
KR (1) KR100561122B1 (enExample)
CN (1) CN1197962C (enExample)
AT (1) ATE530567T1 (enExample)
AU (1) AU741366B2 (enExample)
BR (1) BR9803419B1 (enExample)
CA (1) CA2243985C (enExample)
CZ (1) CZ293217B6 (enExample)
ES (1) ES2374910T3 (enExample)
HU (1) HUP9802024A3 (enExample)
IL (1) IL126100A (enExample)
NO (1) NO327476B1 (enExample)
PL (1) PL328525A1 (enExample)
RU (1) RU2238978C2 (enExample)
TW (1) TW513439B (enExample)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998006736A1 (en) * 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US20030138834A1 (en) * 1998-08-19 2003-07-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US20040121394A1 (en) * 1998-08-19 2004-06-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
WO2001014568A1 (en) * 1999-08-21 2001-03-01 Amersham Pharmacia Biotech Inc. Taq dna polymerases having an amino acid substitution at e681 and homologs thereof exhibiting improved salt tolerance
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
EP2801624B1 (en) * 2001-03-16 2019-03-06 Singular Bio, Inc Arrays and methods of use
WO2003004632A2 (en) * 2001-07-06 2003-01-16 Amersham Biosciences Corp Novel dna polymerases having amino acid substitutions and homologs thereof
EP1474532A4 (en) * 2002-02-12 2005-05-04 Applera Corp POLYMERASE COMPOSITIONS
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
KR100571808B1 (ko) * 2003-04-16 2006-04-17 삼성전자주식회사 다층 박막 구조를 가진 dna 칩
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
EP1766090B1 (en) * 2004-05-25 2011-04-27 Helicos Biosciences Corporation Methods for nucleic acid immobilisation
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7524831B2 (en) * 2005-03-02 2009-04-28 Schering Corporation Treatments for Flaviviridae virus infection
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
GB0518867D0 (en) * 2005-09-15 2005-10-26 Secretary Trade Ind Brit Microscopy tip
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
AU2006331512B2 (en) * 2005-12-22 2012-02-23 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
CA2633520A1 (en) * 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Protein engineering strategies to optimize activity of surface attached proteins
JP2009521227A (ja) * 2005-12-22 2009-06-04 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド ヌクレオチドアナログ組込み用ポリメラーゼ
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
EP2089517A4 (en) * 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
US20100081916A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware. Histological facilitation systems and methods
US20100081915A1 (en) * 2008-09-29 2010-04-01 Searete Llc, Alimited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081924A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081927A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081928A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological Facilitation systems and methods
US20100081926A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
FR2940291B1 (fr) 2008-12-23 2012-12-21 Isp Investments Inc Peptide derive d'hmg-coa reductase et composition cosmetique ou pharmaceutique le contenant
FR2940125B1 (fr) * 2008-12-23 2013-03-22 Isp Investments Inc Composition cosmetique ou pharmaceutique apaisante comprenant un peptide activateur de la hmg-coa reductase
FR2944526B1 (fr) 2009-04-15 2013-05-10 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere
FR2944445B1 (fr) 2009-04-15 2013-08-16 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique apaisant
US8933036B2 (en) 2009-04-15 2015-01-13 Isp Investments Inc. Cosmetic and/or pharmaceutical composition comprising a yeast peptide hydrolysate and use of the yeast peptide hydrolysate as an active agent for strengthening hair
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
WO2011157434A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
EP2582803B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
EP2582806B1 (en) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2564692T3 (es) 2010-06-18 2016-03-28 F. Hoffmann-La Roche Ag ADN polimerasas con discriminación incrementada de no correspondencia 3'
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
EP2675897B1 (en) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
EP2788480B1 (en) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna polymerases with improved activity
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 改善された活性を有するdnaポリメラーゼ
CN105659091A (zh) 2013-08-19 2016-06-08 卓异生物公司 用于单分子检测的测定及其应用
US10329545B2 (en) 2014-11-14 2019-06-25 Illumina, Inc. Polymerases
US10308918B2 (en) 2015-02-02 2019-06-04 Roche Molecular Systems, Inc. Polymerase variants
WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
US10526588B2 (en) * 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
US11046939B2 (en) 2015-11-27 2021-06-29 Kyushu University, National University Corporation DNA polymerase variant
JP7504872B2 (ja) 2018-09-13 2024-06-24 エフ. ホフマン-ラ ロシュ アーゲー 改善されたストランド置換能力を有する変異体dnaポリメラーゼ
EP4308718A4 (en) * 2021-03-19 2025-09-10 Mgi Tech Co Ltd POLYMERASE FOR THE EFFICIENT INCORPORATION OF NUCLEOTIDES WITH 3'-PHOSPHATE AND OTHER 3'-TERMINATORS
WO2026153862A1 (en) 2025-01-14 2026-07-23 F. Hoffmann-La Roche Ag Dna polymerases with improved activity

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
CA2018273C (en) 1989-06-09 1999-04-06 Peter D. Senter Thermally stable cytosine deaminase
US5338678A (en) 1989-06-09 1994-08-16 Oncogen, A Limited Partnership Expression of DNA sequences encoding a thermally stable cytosine deaminase from saccharomyces
US5292658A (en) * 1989-12-29 1994-03-08 University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center Cloning and expressions of Renilla luciferase
US6083686A (en) 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5446591A (en) * 1993-02-08 1995-08-29 Lockheed Missiles & Space Co., Inc. Lens mounting for use with liquid lens elements
US5571706A (en) * 1994-06-17 1996-11-05 The United States Of America As Represented By The Secretary Of Agriculture Plant virus resistance gene and methods
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
DE69400567T2 (de) * 1994-10-17 1997-02-06 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit veränderter Nukleotid-Bindungstelle
US5852191A (en) * 1995-06-07 1998-12-22 Carnegie Mellon University Rigidized monomethine cyanines
US6107029A (en) * 1996-07-31 2000-08-22 Message Pharmaceticals, Inc. Universal method for detecting interactions between RNA molecules and RNA binding proteins
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
US5723298A (en) * 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
JP3435416B2 (ja) 1997-03-12 2003-08-11 ピーイー コーポレイション(エヌワイ) 改善された標識ヌクレオチド取込み特性を有するdnaポリメラーゼ

Also Published As

Publication number Publication date
CN1218832A (zh) 1999-06-09
CZ287498A3 (cs) 1999-03-17
EP0902035A3 (en) 1999-06-23
US20020142333A1 (en) 2002-10-03
US20030152988A1 (en) 2003-08-14
KR19990029676A (ko) 1999-04-26
JP4417452B2 (ja) 2010-02-17
NO327476B1 (no) 2009-07-13
AU741366B2 (en) 2001-11-29
IL126100A (en) 2005-12-18
ES2374910T3 (es) 2012-02-23
HK1018796A1 (en) 2000-01-07
US7598032B2 (en) 2009-10-06
TW513439B (en) 2002-12-11
CZ293217B6 (cs) 2004-03-17
IL126100A0 (en) 1999-05-09
NO984157L (no) 1999-03-12
PL328525A1 (en) 1999-03-15
EP0902035B1 (en) 2011-10-26
CA2243985C (en) 2004-08-10
KR100561122B1 (ko) 2006-07-25
US6346379B1 (en) 2002-02-12
ATE530567T1 (de) 2011-11-15
HU9802024D0 (en) 1998-12-28
RU2238978C2 (ru) 2004-10-27
JPH11137284A (ja) 1999-05-25
EP0902035A2 (en) 1999-03-17
CN1197962C (zh) 2005-04-20
HUP9802024A2 (hu) 1999-05-28
NO984157D0 (no) 1998-09-10
HUP9802024A3 (en) 2001-02-28
BR9803419A (pt) 2000-02-08
AU8416198A (en) 1999-03-25
CA2243985A1 (en) 1999-03-11

Similar Documents

Publication Publication Date Title
NO984157D0 (no) Forandrete termostabile DNA-polymeraser for sekvensbestemmelse
BR9704260B1 (pt) dna polimerase termoestável modificada, método para sua preparação, composição compreendendo a mesma, bem como seqüência de ácido nucléico, vetor, célula hospedeira de microorganismo, método e kit para seqüenciamento.
AU2002321094A1 (en) A novel group of alpha-amylases and a method for identification and production of novel alpha-amylases
BR0010725A (pt) Expressão e exportação de proteìnas de interferon-alfa como proteìnas de fusão de fc
DE69834056D1 (de) De novo oder "universaler" sequenzierungs-array
AU5602298A (en) Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
BR9814404A (pt) " clonagem de expressão em fungos filamentosos "
RU98117244A (ru) Модифицированные термостабильные днк-полимеразы для секвенирования
ATE450603T1 (de) Polypeptide mit verzweigungsenzym-aktivität und für diese kodierende nukleinsäuren
BR9811675A (pt) Genes isolados para proteìna da membrana de célula dentrìtica
KR960010863A (ko) 재조합 머사시딘 및 이의 제조방법
Ingram et al. Studies on the structure of purified alanine and valine transfer RNA from yeast
BR9909012A (pt) Enzima para oxidação de fenol purificada, polinucleotìdeo isolado, vetor de expressão, célula hospedeira, processos para produzir uma enzima para oxidação de fenol e para produzir uma célula hospedeira, cultura substancialmente pura, e, composição de enzima
Tan et al. Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing
WO2004029578A3 (en) Energy transfer dyes, terminators and use thereof
AU7579891A (en) Multichromophore fluorescent probes
BR9909043A (pt) Composição detergente
RU2221866C2 (ru) Очищенная днк-полимераза, рекомбинантная днк-полимераза, способы их получения, выделенная последовательность днк и набор для полимеразной цепной реакции
CN122012450A (zh) 一种海绵末端脱氧核苷酸转移酶及其应用
CN113801877A (zh) 一种蛋白表达方法
KR950005990A (ko) 인간 인터류킨-4(human interleukin-4)의 효모에서의 발현
WO1998014608A1 (en) Method for priming and dna sequencing
KR950018044A (ko) 재조합 유비퀴틴 융합 인간 싸이모신 알파 1의 유전자, 이를 포함하는 발현 벡터 및 그 단백질
BR9810643A (pt) Genes e proteìnas de telomerase de vertebrado, eutilização dos mesmos

Legal Events

Date Code Title Description
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 21/09/2010, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 17A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2344 DE 08-12-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.