EP2205754A2 - Accroissement du rendement en méthionine - Google Patents
Accroissement du rendement en méthionineInfo
- Publication number
- EP2205754A2 EP2205754A2 EP08804748A EP08804748A EP2205754A2 EP 2205754 A2 EP2205754 A2 EP 2205754A2 EP 08804748 A EP08804748 A EP 08804748A EP 08804748 A EP08804748 A EP 08804748A EP 2205754 A2 EP2205754 A2 EP 2205754A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- methionine
- microorganism
- gene
- expression
- reduced
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 title claims abstract description 145
- 229930182817 methionine Natural products 0.000 title claims abstract description 133
- 230000001965 increasing effect Effects 0.000 title claims abstract description 40
- 244000005700 microbiome Species 0.000 claims abstract description 59
- 238000004519 manufacturing process Methods 0.000 claims abstract description 49
- 238000000034 method Methods 0.000 claims abstract description 40
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 34
- 239000001963 growth medium Substances 0.000 claims abstract description 25
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 229910052717 sulfur Inorganic materials 0.000 claims abstract description 13
- 239000011593 sulfur Substances 0.000 claims abstract description 13
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- 238000002955 isolation Methods 0.000 claims abstract description 5
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 101
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 55
- 230000014509 gene expression Effects 0.000 claims description 45
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 44
- 229910019142 PO4 Inorganic materials 0.000 claims description 37
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 37
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- 239000002028 Biomass Substances 0.000 claims description 12
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 12
- 238000003776 cleavage reaction Methods 0.000 claims description 12
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- MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 claims description 9
- 229960001570 ademetionine Drugs 0.000 claims description 9
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 claims description 9
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- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 claims description 7
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- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 6
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 5
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- 229910000037 hydrogen sulfide Inorganic materials 0.000 claims description 5
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- 150000002741 methionine derivatives Chemical class 0.000 claims description 5
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- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
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- 101100175237 Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) gcvPB gene Proteins 0.000 claims description 2
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- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims description 2
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- 229940075933 dithionate Drugs 0.000 claims description 2
- GRWZHXKQBITJKP-UHFFFAOYSA-L dithionite(2-) Chemical compound [O-]S(=O)S([O-])=O GRWZHXKQBITJKP-UHFFFAOYSA-L 0.000 claims description 2
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 53
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/12—Methionine; Cysteine; Cystine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
- C12Y305/0101—Formyltetrahydrofolate deformylase (3.5.1.10)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Catalysts (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK12198802.6T DK2573189T3 (en) | 2007-10-02 | 2008-09-25 | Increase methioninudbytte |
| EP08804748.5A EP2205754B1 (fr) | 2007-10-02 | 2008-09-25 | Accroissement du rendement en méthionine |
| EP12198802.6A EP2573189B1 (fr) | 2007-10-02 | 2008-09-25 | Augmentation du rendement de la méthionine |
| PL08804748T PL2205754T3 (pl) | 2007-10-02 | 2008-09-25 | Zwiększanie wydajności otrzymywania metioniny |
| HRP20160075TT HRP20160075T1 (hr) | 2007-10-02 | 2016-01-25 | Poveä†anje prinosa metionina |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2007/060433 WO2009043372A1 (fr) | 2007-10-02 | 2007-10-02 | Accroissement du rendement en méthionine |
| PCT/EP2008/062859 WO2009043803A2 (fr) | 2007-10-02 | 2008-09-25 | Accroissement du rendement en méthionine |
| EP08804748.5A EP2205754B1 (fr) | 2007-10-02 | 2008-09-25 | Accroissement du rendement en méthionine |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP12198802.6A Division EP2573189B1 (fr) | 2007-10-02 | 2008-09-25 | Augmentation du rendement de la méthionine |
| EP12198802.6A Division-Into EP2573189B1 (fr) | 2007-10-02 | 2008-09-25 | Augmentation du rendement de la méthionine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP2205754A2 true EP2205754A2 (fr) | 2010-07-14 |
| EP2205754B1 EP2205754B1 (fr) | 2015-11-04 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP08804748.5A Not-in-force EP2205754B1 (fr) | 2007-10-02 | 2008-09-25 | Accroissement du rendement en méthionine |
| EP12198802.6A Not-in-force EP2573189B1 (fr) | 2007-10-02 | 2008-09-25 | Augmentation du rendement de la méthionine |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP12198802.6A Not-in-force EP2573189B1 (fr) | 2007-10-02 | 2008-09-25 | Augmentation du rendement de la méthionine |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US20100248311A1 (fr) |
| EP (2) | EP2205754B1 (fr) |
| JP (1) | JP5701604B2 (fr) |
| KR (2) | KR101636662B1 (fr) |
| CN (2) | CN103087971B (fr) |
| AR (1) | AR068722A1 (fr) |
| BR (1) | BRPI0818528B1 (fr) |
| DK (2) | DK2573189T3 (fr) |
| ES (2) | ES2600036T3 (fr) |
| HR (1) | HRP20160075T1 (fr) |
| HU (2) | HUE030032T2 (fr) |
| MX (1) | MX2010003609A (fr) |
| MY (2) | MY170749A (fr) |
| PL (2) | PL2573189T3 (fr) |
| RU (1) | RU2010115225A (fr) |
| WO (2) | WO2009043372A1 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2700715A1 (fr) | 2012-08-20 | 2014-02-26 | Evonik Degussa GmbH | Procédé de fabrication fermentative dýacides aminés L en utilisant des souches améliorées de la famille des enterobacteriaceae |
| EP2762571A1 (fr) | 2013-01-30 | 2014-08-06 | Evonik Industries AG | Microorganisme et procédé de fabrication par fermentation d'acides aminés |
Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4110641B2 (ja) | 1998-11-17 | 2008-07-02 | 味の素株式会社 | 発酵法によるｌ−メチオニンの製造法 |
| BRPI0807760A2 (pt) * | 2007-02-19 | 2014-06-17 | Evonik Degussa Gmbh | Bactérias corineformes com atividade de formato-thf-sintetase e/ou atividade de clivagem de glicina |
| WO2010020289A1 (fr) | 2008-08-22 | 2010-02-25 | Metabolic Explorer | Fabrication d'acides aminés contenant du soufre n-acétylés avec des microorganismes dotés d’une activité enzymatique de n-acétyltransférase accrue |
| WO2010020290A1 (fr) * | 2008-08-22 | 2010-02-25 | Metabolic Explorer | Fabrication de méthionine sans n-acétylméthionine |
| DE102009030342A1 (de) | 2009-06-25 | 2010-12-30 | Evonik Degussa Gmbh | Verfahren zur fermentativen Herstellung von organisch chemischen Verbindungen |
| FR2951195B1 (fr) * | 2009-10-14 | 2014-01-31 | Roquette Freres | Composition riche en methionine destinee a l'alimentation animale |
| KR20160114184A (ko) | 2009-11-18 | 2016-10-04 | 미리안트 코포레이션 | 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 |
| WO2011073738A1 (fr) * | 2009-12-14 | 2011-06-23 | Metabolic Explorer | Utilisation de promoteurs inductibles dans la production de méthionine |
| JP5819316B2 (ja) * | 2009-12-30 | 2015-11-24 | メタボリック エクスプローラー | コハク酸デヒドロゲナーゼを過剰発現させることによるメチオニンの増産 |
| KR101915819B1 (ko) | 2009-12-30 | 2018-11-06 | 에보니크 데구사 게엠베하 | 메티오닌 생산을 위한 균주 및 방법 |
| AR083468A1 (es) * | 2010-10-25 | 2013-02-27 | Metabolic Explorer Sa | Aumento de la disponibilidad de nadph para la produccion de metionina |
| CN103429748B (zh) * | 2010-12-30 | 2016-01-06 | 代谢探索者公司 | 甲硫氨酸羟基类似物(mha)的发酵产生 |
| WO2012090021A1 (fr) | 2010-12-30 | 2012-07-05 | Metabolic Explorer | Micro-organisme recombinant pour la production par fermentation de méthionine |
| EP2479279A1 (fr) * | 2011-01-20 | 2012-07-25 | Evonik Degussa GmbH | Procédé de fabrication fermentative d'acides aminés contenant du soufre |
| US9234223B2 (en) | 2011-04-01 | 2016-01-12 | Ajinomoto Co., Inc. | Method for producing L-cysteine |
| BR112013023465B1 (pt) | 2011-04-01 | 2020-10-27 | Ajinomoto Co., Inc | método para produzir l-cisteína |
| DE102011006716A1 (de) | 2011-04-04 | 2012-10-04 | Evonik Degussa Gmbh | Mikroorganismus und Verfahren zur fermentativen Herstellung einer organisch-chemischen Verbindung |
| DE102011118019A1 (de) | 2011-06-28 | 2013-01-03 | Evonik Degussa Gmbh | Varianten des Promotors des für die Glyzerinaldehyd-3-phosphat-Dehydrogenase kodierenden gap-Gens |
| AR086790A1 (es) * | 2011-06-29 | 2014-01-22 | Metabolic Explorer Sa | Un microorganismo para la produccion de metionina con importacion de glucosa mejorada |
| FR2983870B1 (fr) | 2011-12-08 | 2015-07-17 | Roquette Freres | Composition en methionine destinee a l'alimentation animale |
| EP2628792A1 (fr) * | 2012-02-17 | 2013-08-21 | Evonik Industries AG | Cellule dotée d'une activité ppGppase moindre |
| WO2013160124A1 (fr) | 2012-04-27 | 2013-10-31 | Evonik Industries Ag | Aloha-isopropylmalate synthases résistant à la rétroaction |
| CN104411821B (zh) | 2012-06-18 | 2017-08-08 | 代谢探索者公司 | 用于发酵生产甲硫氨酸的重组微生物 |
| WO2014049382A2 (fr) | 2012-09-26 | 2014-04-03 | Metabolic Explorer | Production de fermentation d'éthylènediamine par un micro-organisme recombinant |
| DE102012024435A1 (de) | 2012-12-14 | 2014-07-10 | Forschungszentrum Jülich GmbH | Verfahren zur Identifizierung einer Zelle mit gegenüber ihrem Wildtyp erhöhten intrazellulären Konzentration eines bestimmten Metaboliten, wobei die Veränderung der Zelle durch Rekombi-neering erreicht wird, sowie ein Verfahren zur Herstellung einer gegenüber ihrem Wildtyp genetisch veränderten Produktionszelle mit optimierter Produktion eines bestimmten Metaboliten, ein Verfahren zur Herstellung dieses Metaboliten, sowie dafür geeignete Nukleinsäuren |
| DK2811028T3 (en) | 2013-06-03 | 2017-05-01 | Evonik Degussa Gmbh | Process for Preparation of L-Valine Using Recombinant Coryn Bacteria Containing the Propionate Inducible IlvBN Operon |
| BR112016004367B1 (pt) | 2013-08-30 | 2022-06-14 | Evonik Operations Gmbh | Microrganismo recombinante e método para a produção fermentativa de metionina e/ou seus derivados |
| EP2940039A1 (fr) * | 2014-04-30 | 2015-11-04 | Evonik Degussa GmbH | Procédé de production d'acides aminés L dans des corynebactéries en utilisant un système de fission de la glycine |
| CN103981206B (zh) * | 2014-06-06 | 2016-03-02 | 中国科学院天津工业生物技术研究所 | 一种筛选高产苏氨酸菌株的方法 |
| MY194223A (en) * | 2014-09-01 | 2022-11-22 | Evonik Operations Gmbh | Method and microorganism for methionine production by fermentation with improved methionine efflux |
| BR112017019631A2 (pt) * | 2015-03-18 | 2018-05-15 | Basf Se | ?microrganismo recombinante, composição, métodos para produzir um microrganismo recombinante, para produzir piruvato, succinato, aspartato, malato, lactato, valina, leucina e/ou alanina, e para cultivar ou desenvolver um microrganismo geneticamente modificado, uso de um microrganismo recombinante, processo para produção fermentativa de piruvato, succinato, aspartato, malato, lactato, valina, leucina e/ou alanina, construção de expressão recombinante, e, vetor recombinante? |
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