ECSP22052002A - Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos - Google Patents

Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos

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Publication number
ECSP22052002A
ECSP22052002A ECSENADI202252002A ECDI202252002A ECSP22052002A EC SP22052002 A ECSP22052002 A EC SP22052002A EC SENADI202252002 A ECSENADI202252002 A EC SENADI202252002A EC DI202252002 A ECDI202252002 A EC DI202252002A EC SP22052002 A ECSP22052002 A EC SP22052002A
Authority
EC
Ecuador
Prior art keywords
cell
culture
protease
applications
hydrolysate
Prior art date
Application number
ECSENADI202252002A
Other languages
English (en)
Inventor
Kai Yi Carrie Chan
Chun Hei Poon
Po San Mario Chin
Original Assignee
Avant Meats Company Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avant Meats Company Ltd filed Critical Avant Meats Company Ltd
Publication of ECSP22052002A publication Critical patent/ECSP22052002A/es

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    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/14Bags
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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Abstract

Un método para generar hidrolizado de células mediante cultivo celular in vitro incluye los pasos de lisar una célula usando sonicación para liberar proteínas en la célula; digerir las proteínas usando proteasa para producir péptidos cortos que tienen un tamaño molecular inferior a 500 daltons (Da); terminar el paso de digestión calentando la proteasa a una temperatura predeterminada durante un período de tiempo predeterminado o diluyendo la proteasa a una concentración predeterminada, y filtrando de manera estéril la mezcla del paso de terminación de la digestión a través de un filtro de membrana. El hidrolizado de células generado se puede usar en varios productos que incluyen, entre otros, cuidado de la piel, cuidado de heridas, cosméticos, productos alimenticios, suplementos, medicamentos y otras aplicaciones medicinales.
ECSENADI202252002A 2019-12-02 2022-07-01 Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos ECSP22052002A (es)

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US201962942568P 2019-12-02 2019-12-02

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ECSP22052002A true ECSP22052002A (es) 2022-09-30

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ECSENADI202251779A ECSP22051779A (es) 2019-12-02 2022-07-01 Métodos para mejorar el cultivo celular con proteínas específicas de cada especie o específicas de cada género y las aplicaciones de los mismos
ECSENADI202252002A ECSP22052002A (es) 2019-12-02 2022-07-01 Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos
ECSENADI202251806A ECSP22051806A (es) 2019-12-02 2022-07-01 Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células
ECSENADI202251770A ECSP22051770A (es) 2019-12-02 2022-07-01 Métodos de producción de carne por cultivo celular in vitro

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ECSENADI202251779A ECSP22051779A (es) 2019-12-02 2022-07-01 Métodos para mejorar el cultivo celular con proteínas específicas de cada especie o específicas de cada género y las aplicaciones de los mismos

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ECSENADI202251806A ECSP22051806A (es) 2019-12-02 2022-07-01 Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células
ECSENADI202251770A ECSP22051770A (es) 2019-12-02 2022-07-01 Métodos de producción de carne por cultivo celular in vitro

Country Status (16)

Country Link
US (3) US20240093153A1 (es)
EP (5) EP4069824A1 (es)
JP (5) JP2023507242A (es)
KR (5) KR20220110798A (es)
CN (5) CN115443330A (es)
AU (5) AU2020396360A1 (es)
BR (4) BR112022010814A2 (es)
CA (5) CA3163651A1 (es)
CO (4) CO2022009121A2 (es)
EC (4) ECSP22051779A (es)
IL (4) IL293492A (es)
MX (4) MX2022006590A (es)
PE (4) PE20221184A1 (es)
PH (4) PH12022551329A1 (es)
TW (1) TW202322704A (es)
WO (5) WO2021111196A1 (es)

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MX2022006590A (es) * 2019-12-02 2022-09-19 Avant Meats Company Ltd Metodos de produccion de carne por cultivo celular in vitro.
US11319520B1 (en) 2021-09-21 2022-05-03 Upside Foods, Inc. Filter cake-based systems and methods for the cultivation of cells and cell biomass
CN114181888B (zh) * 2021-11-24 2023-12-08 汕头大学 一种浅色黄姑鱼鱼鳔细胞系的建立方法及应用
CN119278210A (zh) * 2022-06-02 2025-01-07 奥方特蛋白质科技有限公司 基因工程酵母细胞、其产生的重组蛋白及其应用
WO2024028439A1 (en) 2022-08-03 2024-02-08 Novelty For Them Gmbh Complexed asthaxanthin undergoing a hypsochromic shift upon heating and application in food products
WO2024134658A1 (en) * 2022-12-22 2024-06-27 Sea2Cell Ltd. Compositions and methods for culturing cells of aquatic species
CN116751742B (zh) * 2023-06-29 2025-01-03 中国海洋大学 一种三文鱼肌肉卫星细胞提取方法
WO2025048697A1 (en) * 2023-08-25 2025-03-06 Re:Meat Ab A method for cultivating food and beverages in a bioreactor
CN117770404B (zh) * 2023-12-14 2026-04-21 中国海洋大学 一种基于3d打印的细胞培养鱼肉及其制备方法

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3418208A (en) * 1966-02-28 1968-12-24 Mobil Oil Corp Growing increased yields of micro-organisms
FR2594847B1 (fr) * 1986-02-25 1989-06-02 Moet Hennessy Rech Procede de preparation de polypeptides biologiquement actifs, polypeptides obtenus et compositions les contenant
DE3641824A1 (de) * 1986-12-06 1988-06-16 Bayer Ag Verfahren und vorrichtung zur kultivierung immobilisierter oder adhaerierter saeugerzellen sowie dessen verwendung bei der herstellung von proteinen
KR0131822B1 (ko) * 1988-05-23 1998-04-11 죤 에프. 튜은트 바이오 반응기 장치
ATE307599T1 (de) * 1991-11-20 2005-11-15 Innogenetics Nv Keratinozyt-abstammende granulate zur verwendung als wundheilendes mittel
WO1999031222A1 (en) * 1997-12-18 1999-06-24 Willem Frederik Van Eelen Industrial scale production of meat from in vitro cell cultures
US8026096B1 (en) * 1998-10-08 2011-09-27 Protein Sciences Corporation In vivo active erythropoietin produced in insect cells
US6664104B2 (en) * 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
US20070122904A1 (en) 2000-09-29 2007-05-31 Unisearch Limited Method and apparatus for culturing cells
EP1470235A4 (en) * 2001-07-13 2008-05-21 Univ Oklahoma GLYCOSAMINOGLYCANPOLYMERS AND METHOD FOR THE PRODUCTION AND USE THEREOF
JP2003047461A (ja) * 2001-07-31 2003-02-18 Astec:Kk アパタイトシ−トを用いた細胞の高密度細胞培養法及び培養装置、細胞培養モジュール
CN100369630C (zh) * 2002-12-03 2008-02-20 细胞移种有限公司 细胞裂解物的药用组合物及其生产和使用方法
WO2004072286A1 (ja) * 2003-01-23 2004-08-26 Ono Pharmaceutical Co., Ltd. ヒトpd−1に対し特異性を有する物質
WO2005121316A1 (en) * 2004-06-11 2005-12-22 Bernard O'brien Institute Of Microsurgery Tissue material and muscle derived matrix
EP1789063B1 (en) * 2004-09-17 2011-12-14 Jon Vein Tissue engineered meat for consumption and a method for producing tissue engineered meat for consumption
ATE496987T1 (de) * 2006-05-22 2011-02-15 Biovest Int Inc Ausserkapilläres fluidzirkulationssystem und - verfahren
WO2008109880A1 (en) * 2007-03-08 2008-09-12 The Johns Hopkins University Bioreactor system for pharmacokinetic-pharmacodynamic analysis
US20080254167A1 (en) * 2007-04-10 2008-10-16 Solae, Llc Seafood Compositions Comprising Structured Protein Products
WO2009016078A2 (en) * 2007-07-31 2009-02-05 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for culturing mammalian stem cells
CN101918532B (zh) * 2007-08-22 2016-03-30 普罗柏欧贞股份公司 用于体外测试免疫原性和免疫功能的培养系统和方法
EP2356213B1 (en) * 2008-11-04 2019-05-29 Viacyte, Inc. Stem cell aggregate suspension compositions and methods for differentiation thereof
WO2011002926A2 (en) * 2009-07-01 2011-01-06 The General Hospital Corporation Isolated adult cells, artificial organs,rehabilitated organs, research rools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same
US20110091604A1 (en) * 2009-10-21 2011-04-21 Seth Adrian Miller Synthetic meat
US8592151B2 (en) * 2009-11-17 2013-11-26 Musc Foundation For Research Development Assessing left ventricular remodeling via temporal detection and measurement of microRNA in body fluids
WO2011103359A2 (en) * 2010-02-17 2011-08-25 Inq Biosciences Corporation Culture systems, apparatus, and related methods and articles
GB201019932D0 (en) * 2010-11-24 2011-01-05 Imp Innovations Ltd Dimensional hollow fibre bioreactor systems for the maintenance, expansion, differentiation and harvesting of human stem cells and their progeny
WO2012103922A1 (en) * 2011-01-31 2012-08-09 Red Patent B.V. Installation and method for biomass conversion into methane
AU2012271329A1 (en) * 2011-06-17 2013-12-19 Amgen Inc. Method of treating or ameliorating metabolic disorders using Clec-2
WO2014028642A1 (en) * 2012-08-17 2014-02-20 Easel Biotechnologies, Llc Two stage production of higher alcohols
BR112016009803B1 (pt) * 2013-10-30 2022-02-08 The Curators Of The University Of Missouri Método para especificação e cultivo de linhagem muscular esquelética escalonável
CN103992385B (zh) 2014-05-22 2017-05-10 浙江海洋学院 一种大黄鱼鱼鳔抗氧化胶原肽及其制备方法和用途
CN104109705B (zh) * 2014-06-19 2017-10-31 广东省农业科学院蚕业与农产品加工研究所 一种酶法制备具有促进伤口愈合活性的海洋胶原蛋白肽的方法
CN104313098A (zh) 2014-10-28 2015-01-28 威海市桢昊生物技术有限公司 一种鱼鳔胶原蛋白粉的制取方法
BR112017016986A2 (pt) * 2015-02-09 2018-04-03 Univercells Nv sistema, aparelho e método para a produção de células e/ou produtos de célula
CN105219827B (zh) 2015-11-10 2020-09-01 浙江海洋学院 鱼鳔胶原蛋白多功能肽的制备方法
IL302693B2 (en) * 2016-07-11 2025-10-01 Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd Methods and systems for growing cells in culture
NZ750365A (en) * 2016-07-28 2022-07-01 Fonterra Cooperative Group Ltd Dairy product and process
CN106749634B (zh) * 2017-01-17 2020-09-15 北京鸿顺养源生物科技有限公司 一种具有抗氧化活性的猪血红蛋白糖肽及其制备方法与应用
CN106805252A (zh) * 2017-01-22 2017-06-09 福州大学 一种鱼短肽及其在营养强化剂中的应用
CA3066060A1 (en) * 2017-06-07 2018-12-13 Wild Type, Inc. Ex vivo meat production
CA3070048A1 (en) * 2017-07-15 2019-01-24 Aleph Farms Ltd. Cultured meat compositions
CN107400633B (zh) * 2017-08-17 2023-10-10 上海白泽医疗器械有限公司 中空纤维交换器及中空纤维交换式培养系统
JP6279801B1 (ja) * 2017-09-19 2018-02-14 株式会社らいむ 組成物およびその製造方法
US10669524B2 (en) * 2018-06-12 2020-06-02 Fork & Goode, Inc. Large scale cell culture system for making meat and associated products
CN108823072B (zh) * 2018-09-19 2023-08-15 青岛中科蓝智生物科技发展有限公司 一种微藻细胞的培养-采收一体化系统及方法
CN110117527A (zh) 2019-05-15 2019-08-13 刘宝全 一种干细胞代谢废物的强化排出方法
CN110257227B (zh) * 2019-06-20 2020-08-04 江南大学 一种用于动物细胞培养的生物反应器
CN110201000A (zh) * 2019-07-11 2019-09-06 广东海洋大学 一种具有促进创伤愈合作用的方格星虫酶解蛋白肽粉的制备方法及其应用
CN110317848B (zh) * 2019-08-08 2020-02-18 北京工商大学 一种胶原蛋白肽的制备方法
US11306342B2 (en) 2019-12-02 2022-04-19 Avant Meats Company Limited Cell hydrolysate composition from cultivated cells and applications thereof
MX2022006590A (es) * 2019-12-02 2022-09-19 Avant Meats Company Ltd Metodos de produccion de carne por cultivo celular in vitro.

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