ECSP22052002A - Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos - Google Patents

Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos

Info

Publication number
ECSP22052002A
ECSP22052002A ECSENADI202252002A ECDI202252002A ECSP22052002A EC SP22052002 A ECSP22052002 A EC SP22052002A EC SENADI202252002 A ECSENADI202252002 A EC SENADI202252002A EC DI202252002 A ECDI202252002 A EC DI202252002A EC SP22052002 A ECSP22052002 A EC SP22052002A
Authority
EC
Ecuador
Prior art keywords
cell
culture
protease
applications
hydrolysate
Prior art date
Application number
ECSENADI202252002A
Other languages
English (en)
Inventor
Kai Yi Carrie Chan
Chun Hei Poon
Po San Mario Chin
Original Assignee
Avant Meats Company Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avant Meats Company Ltd filed Critical Avant Meats Company Ltd
Publication of ECSP22052002A publication Critical patent/ECSP22052002A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2311—Interleukin-11 (IL-11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Rheumatology (AREA)
  • Transplantation (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Dermatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método para generar hidrolizado de células mediante cultivo celular in vitro incluye los pasos de lisar una célula usando sonicación para liberar proteínas en la célula; digerir las proteínas usando proteasa para producir péptidos cortos que tienen un tamaño molecular inferior a 500 daltons (Da); terminar el paso de digestión calentando la proteasa a una temperatura predeterminada durante un período de tiempo predeterminado o diluyendo la proteasa a una concentración predeterminada, y filtrando de manera estéril la mezcla del paso de terminación de la digestión a través de un filtro de membrana. El hidrolizado de células generado se puede usar en varios productos que incluyen, entre otros, cuidado de la piel, cuidado de heridas, cosméticos, productos alimenticios, suplementos, medicamentos y otras aplicaciones medicinales.
ECSENADI202252002A 2019-12-02 2022-07-01 Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos ECSP22052002A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201962942568P 2019-12-02 2019-12-02

Publications (1)

Publication Number Publication Date
ECSP22052002A true ECSP22052002A (es) 2022-09-30

Family

ID=76221512

Family Applications (4)

Application Number Title Priority Date Filing Date
ECSENADI202252002A ECSP22052002A (es) 2019-12-02 2022-07-01 Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos
ECSENADI202251770A ECSP22051770A (es) 2019-12-02 2022-07-01 Métodos de producción de carne por cultivo celular in vitro
ECSENADI202251779A ECSP22051779A (es) 2019-12-02 2022-07-01 Métodos para mejorar el cultivo celular con proteínas específicas de cada especie o específicas de cada género y las aplicaciones de los mismos
ECSENADI202251806A ECSP22051806A (es) 2019-12-02 2022-07-01 Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células

Family Applications After (3)

Application Number Title Priority Date Filing Date
ECSENADI202251770A ECSP22051770A (es) 2019-12-02 2022-07-01 Métodos de producción de carne por cultivo celular in vitro
ECSENADI202251779A ECSP22051779A (es) 2019-12-02 2022-07-01 Métodos para mejorar el cultivo celular con proteínas específicas de cada especie o específicas de cada género y las aplicaciones de los mismos
ECSENADI202251806A ECSP22051806A (es) 2019-12-02 2022-07-01 Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células

Country Status (15)

Country Link
US (3) US20240093153A1 (es)
EP (5) EP4069824A1 (es)
JP (4) JP2023507242A (es)
KR (5) KR20220110798A (es)
CN (5) CN115443330A (es)
AU (5) AU2020396360A1 (es)
BR (4) BR112022010814A2 (es)
CA (5) CA3163651A1 (es)
CO (4) CO2022009121A2 (es)
EC (4) ECSP22052002A (es)
IL (4) IL293492A (es)
MX (4) MX2022006590A (es)
PE (4) PE20221184A1 (es)
TW (1) TW202322704A (es)
WO (5) WO2021111196A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023507242A (ja) * 2019-12-02 2023-02-21 アヴァント ミーツ カンパニー リミテッド 生体外細胞培養による食肉生産方法
CN114181888B (zh) * 2021-11-24 2023-12-08 汕头大学 一种浅色黄姑鱼鱼鳔细胞系的建立方法及应用
WO2023233340A1 (en) * 2022-06-02 2023-12-07 Avant Meats Company Limited Genetically engineered yeast cells, recombinant proteins produced thereof and applications thereof
WO2024028439A1 (en) 2022-08-03 2024-02-08 Novelty For Them Gmbh Complexed asthaxanthin undergoing a hypsochromic shift upon heating and application in food products
WO2024134658A1 (en) * 2022-12-22 2024-06-27 Sea2Cell Ltd. Compositions and methods for culturing cells of aquatic species

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3418208A (en) * 1966-02-28 1968-12-24 Mobil Oil Corp Growing increased yields of micro-organisms
FR2594847B1 (fr) * 1986-02-25 1989-06-02 Moet Hennessy Rech Procede de preparation de polypeptides biologiquement actifs, polypeptides obtenus et compositions les contenant
WO1993010217A1 (en) * 1991-11-20 1993-05-27 N.V. Innogenetics S.A. New cultures of keratinocytes, process for their preparation and their use as wound healing substances
WO1999031222A1 (en) * 1997-12-18 1999-06-24 Willem Frederik Van Eelen Industrial scale production of meat from in vitro cell cultures
US6887693B2 (en) * 1998-12-24 2005-05-03 Cepheid Device and method for lysing cells, spores, or microorganisms
US20070122904A1 (en) * 2000-09-29 2007-05-31 Unisearch Limited Method and apparatus for culturing cells
AU2002361549A1 (en) * 2001-07-13 2003-04-14 Paul L. Deangelis Glycosaminoglycan polymers and methods of making and using same
EP1603594A1 (en) * 2002-12-03 2005-12-14 Innogenetics N.V. Pharmaceutical compositions of cell lysate and processes for the production and use thereof
WO2005121316A1 (en) * 2004-06-11 2005-12-22 Bernard O'brien Institute Of Microsurgery Tissue material and muscle derived matrix
ATE536750T1 (de) * 2004-09-17 2011-12-15 Jon Vein Gewebegezüchtetes fleisch zum verzehr und herstellungsverfahren für gewebegezüchtetes fleisch zum verzehr
EP2404991A3 (en) * 2006-05-22 2013-05-22 Biovest International, Inc. Method and system for the production of cells and cell products and applications thereof
US20080254167A1 (en) * 2007-04-10 2008-10-16 Solae, Llc Seafood Compositions Comprising Structured Protein Products
AU2008363829B2 (en) * 2008-11-04 2014-11-20 Viacyte, Inc. Stem cell aggregate suspension compositions and methods for differentiation thereof
WO2011002926A2 (en) * 2009-07-01 2011-01-06 The General Hospital Corporation Isolated adult cells, artificial organs,rehabilitated organs, research rools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same
WO2011103359A2 (en) * 2010-02-17 2011-08-25 Inq Biosciences Corporation Culture systems, apparatus, and related methods and articles
US20140206056A1 (en) * 2011-01-31 2014-07-24 Red Patent B.V. Installation and method for biomass conversion into methane
WO2014028642A1 (en) * 2012-08-17 2014-02-20 Easel Biotechnologies, Llc Two stage production of higher alcohols
CA2928726A1 (en) * 2013-10-30 2015-05-07 The Curators Of The University Of Missouri Method for scalable skeletal muscle lineage specification and cultivation
CN104109705B (zh) * 2014-06-19 2017-10-31 广东省农业科学院蚕业与农产品加工研究所 一种酶法制备具有促进伤口愈合活性的海洋胶原蛋白肽的方法
AU2016218037B2 (en) * 2015-02-09 2020-12-17 Univercells Technologies S.A. System, apparatus and method for the production of cells and/or cell products
IL302693A (en) * 2016-07-11 2023-07-01 Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd Methods and systems for growing cells in culture
RU2019105567A (ru) * 2016-07-28 2020-08-28 Фонтерра Ко-Оперэйтив Груп Лимитед Молочный продукт и способ
CN106749634B (zh) * 2017-01-17 2020-09-15 北京鸿顺养源生物科技有限公司 一种具有抗氧化活性的猪血红蛋白糖肽及其制备方法与应用
CN106805252A (zh) * 2017-01-22 2017-06-09 福州大学 一种鱼短肽及其在营养强化剂中的应用
JP2020523015A (ja) * 2017-06-07 2020-08-06 ワイルド タイプ，インク． エクスビボでの食肉の生産
EP3728560A4 (en) * 2017-07-15 2021-04-14 Aleph Farms Ltd. MEAT COMPOSITIONS IN CULTURE
CN107400633B (zh) * 2017-08-17 2023-10-10 上海白泽医疗器械有限公司 中空纤维交换器及中空纤维交换式培养系统
CN110117527A (zh) * 2019-05-15 2019-08-13 刘宝全 一种干细胞代谢废物的强化排出方法
CN110257227B (zh) * 2019-06-20 2020-08-04 江南大学 一种用于动物细胞培养的生物反应器
CN110201000A (zh) * 2019-07-11 2019-09-06 广东海洋大学 一种具有促进创伤愈合作用的方格星虫酶解蛋白肽粉的制备方法及其应用
CN110317848B (zh) * 2019-08-08 2020-02-18 北京工商大学 一种胶原蛋白肽的制备方法
JP2023507242A (ja) * 2019-12-02 2023-02-21 アヴァント ミーツ カンパニー リミテッド 生体外細胞培養による食肉生産方法

Also Published As

Publication number Publication date
KR20220110265A (ko) 2022-08-05
CA3163651A1 (en) 2021-06-10
EP4069821A1 (en) 2022-10-12
JP2023504216A (ja) 2023-02-01
PE20221274A1 (es) 2022-09-01
CN117957304A (zh) 2024-04-30
BR112022010816A2 (pt) 2022-08-23
IL293493A (en) 2022-08-01
US20240093153A1 (en) 2024-03-21
MX2022006592A (es) 2022-08-25
JP2023504217A (ja) 2023-02-01
CA3163662A1 (en) 2021-06-10
WO2021111196A1 (en) 2021-06-10
EP4069824A1 (en) 2022-10-12
KR20220110798A (ko) 2022-08-09
ECSP22051770A (es) 2022-09-30
WO2021111263A1 (en) 2021-06-10
US20220025310A1 (en) 2022-01-27
PE20221185A1 (es) 2022-08-05
ECSP22051806A (es) 2022-09-30
WO2023012523A1 (en) 2023-02-09
AU2022324741A1 (en) 2024-02-15
WO2021111270A1 (en) 2021-06-10
CA3173727A1 (en) 2021-06-10
CN115443072A (zh) 2022-12-06
CO2022009114A2 (es) 2022-09-09
TW202322704A (zh) 2023-06-16
EP4068987A4 (en) 2024-03-06
AU2020396377A1 (en) 2022-06-16
IL293495A (en) 2022-08-01
KR20240041372A (ko) 2024-03-29
CO2022009121A2 (es) 2022-09-09
IL293494A (en) 2022-08-01
PE20221183A1 (es) 2022-08-05
JP2023507242A (ja) 2023-02-21
CA3163663A1 (en) 2021-06-10
MX2022006593A (es) 2022-08-25
MX2022006590A (es) 2022-09-19
AU2020397620A1 (en) 2022-06-16
JP2023504218A (ja) 2023-02-01
BR112022010817A2 (pt) 2022-08-23
EP4069861A4 (en) 2024-06-26
CN115443330A (zh) 2022-12-06
EP4068987A1 (en) 2022-10-12
CO2022009119A2 (es) 2022-09-09
EP4381044A1 (en) 2024-06-12
AU2020396360A1 (en) 2022-06-16
KR20220110541A (ko) 2022-08-08
CN115298319A (zh) 2022-11-04
BR112022010822A2 (pt) 2022-08-23
PE20221184A1 (es) 2022-08-05
ECSP22051779A (es) 2022-09-30
EP4069861A1 (en) 2022-10-12
CN115397969A (zh) 2022-11-25
AU2020398072A1 (en) 2022-06-16
KR20220111310A (ko) 2022-08-09
BR112022010814A2 (pt) 2022-08-23
WO2021111219A1 (en) 2021-06-10
CO2022009288A2 (es) 2022-09-09
CA3228282A1 (en) 2023-02-09
IL293492A (en) 2022-08-01
US20220298480A1 (en) 2022-09-22
EP4069821A4 (en) 2024-04-03
MX2022006591A (es) 2022-08-25

Similar Documents

Publication Publication Date Title
ECSP22052002A (es) Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos
CN104042548B (zh) 一种蜗牛黏液提取物及其制备方法和在化妆品中的应用
Munoz-Perez et al. Mesenchymal stromal cell secretome for the treatment of immune-mediated inflammatory diseases: latest trends in isolation, content optimization and delivery avenues
Hong et al. Anti-oxidative and anti-aging activities of porcine by-product collagen hydrolysates produced by commercial proteases: Effect of hydrolysis and ultrafiltration
Jeevithan et al. Type II collagen and gelatin from silvertip shark (Carcharhinus albimarginatus) cartilage: Isolation, purification, physicochemical and antioxidant properties
Zhang et al. Fish scale valorization by hydrothermal pretreatment followed by enzymatic hydrolysis for gelatin hydrolysate production
Gomes et al. Biotechnology applied to cosmetics and aesthetic medicines
CN103243145B (zh) 一种高纯度丝素肽的制备方法
BRPI0517514A (pt) vesìculas bacterianas imunogênicas com proteìnas da membrana externa
Behan et al. Methacrylated cartilage ECM-based hydrogels as injectables and bioinks for cartilage tissue engineering
CN105112363B (zh) 一种人脂肪间充质干细胞的无血清培养基及其制备方法
Xu et al. Effect of marine collagen peptides on physiological and neurobehavioral development of male rats with perinatal asphyxia
CY1123467T1 (el) Θεραπευτικο εμβολιο για τη θεραπεια του παιδικου διαβητη τυπου 1, εφαρμογη του κυτταροδιαχωριστη κυτταρομετριας ροης και της μεθοδου πολλαπλασιασμου των κυτταρων treg για την παραγωγη θεραπευτικου εμβολιου για τη θεραπεια του διαβητη τυπου 1
CN107362356A (zh) 一种具有止血功效的牦牛皮胶原蛋白产品及其制备方法
Zhang et al. Pepsin-soluble collagen from the skin of lophius litulo: a preliminary study evaluating physicochemical, antioxidant, and wound healing properties
Pertea et al. Efficiency of bromelain-enriched enzyme mixture (NexoBrid™) in the treatment of burn wounds
Mao et al. Effect of yak milk casein hydrolysate on Th1/Th2 cytokines production by murine spleen lymphocytes in vitro
TW200504216A (en) Immunization of fish with plant-expressed recombinant proteins
Katsinas et al. Olive pomace phenolic compounds: From an agro-industrial by-product to a promising ocular surface protection for dry eye disease
Zhang et al. The Application of Mesenchymal Stem Cells in Future Vaccine Synthesis
MX2023002080A (es) Vector de poxviridae recombinante que expresa moleculas coestimuladoras.
Doan et al. Protein Extraction from Spirulina Platensis with The Cellulase Enzyme Assistance
Zhu et al. The Combined Anti-Aging Effect of Hydrolyzed Collagen Oligopeptides and Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells on Human Skin Fibroblasts
AR123474A1 (es) Vesículas de la membrana externa
CN104873448A (zh) 美白淡斑中药面膜及其制备方法