BR112022010814A2 - Métodos de produção de carne por cultivo de células in vitro - Google Patents

Métodos de produção de carne por cultivo de células in vitro

Info

Publication number
BR112022010814A2
BR112022010814A2 BR112022010814A BR112022010814A BR112022010814A2 BR 112022010814 A2 BR112022010814 A2 BR 112022010814A2 BR 112022010814 A BR112022010814 A BR 112022010814A BR 112022010814 A BR112022010814 A BR 112022010814A BR 112022010814 A2 BR112022010814 A2 BR 112022010814A2
Authority
BR
Brazil
Prior art keywords
cells
vitro cell
cell culture
animal
culture
Prior art date
Application number
BR112022010814A
Other languages
English (en)
Inventor
San Mario Chin Po
Yi Carrie Chan Kai
Hei Poon Chun
Original Assignee
Avant Meats Company Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avant Meats Company Ltd filed Critical Avant Meats Company Ltd
Publication of BR112022010814A2 publication Critical patent/BR112022010814A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2311—Interleukin-11 (IL-11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Rheumatology (AREA)
  • Transplantation (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MÉTODOS DE PRODUÇÃO DE CARNE POR CULTIVO DE CÉLULAS IN VITRO. Trata-se de um método para produção de carne por cultura de células in vitro que inclui isolar tecido de uma fonte animal ou vegetal e produzir uma suspensão celular de células e cultivar as células em uma estrutura sólida ou semissólida que imita um órgão animal, cultivando-se as células em uma matriz (scaffold) de grau alimentício em um meio de cultura. A expressão de uma ou mais proteínas nas células em crescimento pode ser aumentada alterando-se um nível de um ou mais microRNAs que regulam a expressão da proteína. Além disso, as células em crescimento podem ser cocultivadas com células biologicamente modificadas que secretam fatores de crescimento e citocinas que suportam o crescimento das células in situ. A técnica de cocultivo reduz ou elimina a necessidade de soro fetal bovino derivado de animais no meio de cultura.
BR112022010814A 2019-12-02 2020-07-28 Métodos de produção de carne por cultivo de células in vitro BR112022010814A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962942568P 2019-12-02 2019-12-02
PCT/IB2020/057119 WO2021111196A1 (en) 2019-12-02 2020-07-28 Methods of meat production by in vitro cell cultivation

Publications (1)

Publication Number Publication Date
BR112022010814A2 true BR112022010814A2 (pt) 2022-08-23

Family

ID=76221512

Family Applications (4)

Application Number Title Priority Date Filing Date
BR112022010814A BR112022010814A2 (pt) 2019-12-02 2020-07-28 Métodos de produção de carne por cultivo de células in vitro
BR112022010822A BR112022010822A2 (pt) 2019-12-02 2020-11-14 Métodos para cultivar células, preparação de hidrolisado a partir de células e aplicações dos mesmos
BR112022010816A BR112022010816A2 (pt) 2019-12-02 2020-11-27 Métodos para melhoramento de crescimento celular com proteínas específicas de espécie ou específicas de gênero e aplicações dos mesmos
BR112022010817A BR112022010817A2 (pt) 2019-12-02 2020-11-30 Sistema de produção de carnes cultivadas, tecidos e produtos associados a partir de células

Family Applications After (3)

Application Number Title Priority Date Filing Date
BR112022010822A BR112022010822A2 (pt) 2019-12-02 2020-11-14 Métodos para cultivar células, preparação de hidrolisado a partir de células e aplicações dos mesmos
BR112022010816A BR112022010816A2 (pt) 2019-12-02 2020-11-27 Métodos para melhoramento de crescimento celular com proteínas específicas de espécie ou específicas de gênero e aplicações dos mesmos
BR112022010817A BR112022010817A2 (pt) 2019-12-02 2020-11-30 Sistema de produção de carnes cultivadas, tecidos e produtos associados a partir de células

Country Status (15)

Country Link
US (3) US20240093153A1 (pt)
EP (5) EP4069824A1 (pt)
JP (4) JP2023507242A (pt)
KR (5) KR20220110798A (pt)
CN (5) CN115443330A (pt)
AU (5) AU2020396360A1 (pt)
BR (4) BR112022010814A2 (pt)
CA (5) CA3163651A1 (pt)
CO (4) CO2022009114A2 (pt)
EC (4) ECSP22051770A (pt)
IL (4) IL293492A (pt)
MX (4) MX2022006590A (pt)
PE (4) PE20221184A1 (pt)
TW (1) TW202322704A (pt)
WO (5) WO2021111196A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4069824A1 (en) * 2019-12-02 2022-10-12 Avant Meats Company Limited Methods of meat production by in vitro cell cultivation
CN114181888B (zh) * 2021-11-24 2023-12-08 汕头大学 一种浅色黄姑鱼鱼鳔细胞系的建立方法及应用
WO2023233340A1 (en) * 2022-06-02 2023-12-07 Avant Meats Company Limited Genetically engineered yeast cells, recombinant proteins produced thereof and applications thereof
WO2024028439A1 (en) 2022-08-03 2024-02-08 Novelty For Them Gmbh Complexed asthaxanthin undergoing a hypsochromic shift upon heating and application in food products

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3418208A (en) * 1966-02-28 1968-12-24 Mobil Oil Corp Growing increased yields of micro-organisms
FR2594847B1 (fr) * 1986-02-25 1989-06-02 Moet Hennessy Rech Procede de preparation de polypeptides biologiquement actifs, polypeptides obtenus et compositions les contenant
DK0615545T3 (da) * 1991-11-20 2000-10-30 Innogenetics Nv Lysater hidrørende fra keratinocytter til brug som sårhelingsmidler
WO1999031222A1 (en) * 1997-12-18 1999-06-24 Willem Frederik Van Eelen Industrial scale production of meat from in vitro cell cultures
US6664104B2 (en) * 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
US20070122904A1 (en) * 2000-09-29 2007-05-31 Unisearch Limited Method and apparatus for culturing cells
AU2002361549A1 (en) * 2001-07-13 2003-04-14 Paul L. Deangelis Glycosaminoglycan polymers and methods of making and using same
AU2003298319A1 (en) * 2002-12-03 2004-06-23 Celltran Limited Pharmaceutical compositions of cell lysate and processes for the production and use thereof
US20060153797A1 (en) * 2004-06-11 2006-07-13 Bortolotto Susan K Tissue material and matrix
AU2004324099B2 (en) * 2004-09-17 2011-02-03 Jon Vein Tissue engineered meat for consumption and a method for producing tissue engineered meat for consumption
WO2007139748A2 (en) * 2006-05-22 2007-12-06 Biovest International Inc. Extra-capillary fluid cycling system and method for a cell culture device
US20080254167A1 (en) * 2007-04-10 2008-10-16 Solae, Llc Seafood Compositions Comprising Structured Protein Products
CA2742583C (en) * 2008-11-04 2022-09-27 Viacyte, Inc. Stem cell aggregate suspension compositions and methods for differentiation thereof
US20130323708A1 (en) * 2009-07-01 2013-12-05 Massachusetts Institute Of Technology Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same
WO2011103359A2 (en) * 2010-02-17 2011-08-25 Inq Biosciences Corporation Culture systems, apparatus, and related methods and articles
WO2012103922A1 (en) * 2011-01-31 2012-08-09 Red Patent B.V. Installation and method for biomass conversion into methane
EP2885415B1 (en) * 2012-08-17 2022-08-03 Easel Biotechnologies LLC Two stage production of higher alcohols
CN105899079A (zh) * 2013-10-30 2016-08-24 密苏里大学的策展人 可扩展性骨骼肌谱系特化和培育的方法
CN104109705B (zh) * 2014-06-19 2017-10-31 广东省农业科学院蚕业与农产品加工研究所 一种酶法制备具有促进伤口愈合活性的海洋胶原蛋白肽的方法
US20180030398A1 (en) * 2015-02-09 2018-02-01 Univercells Nv System, apparatus and method for the production of cells and/or cell products
CN109714962A (zh) * 2016-07-11 2019-05-03 耶路撒冷希伯来大学的益生研究开发有限公司 用于在体外培养细胞的系统和方法
MX2019000881A (es) * 2016-07-28 2019-07-01 Fonterra Cooperative Group Ltd Producto lacteo y proceso.
CN106749634B (zh) * 2017-01-17 2020-09-15 北京鸿顺养源生物科技有限公司 一种具有抗氧化活性的猪血红蛋白糖肽及其制备方法与应用
CN106805252A (zh) * 2017-01-22 2017-06-09 福州大学 一种鱼短肽及其在营养强化剂中的应用
IL308692A (en) * 2017-06-07 2024-01-01 Wild Type Inc Meat production outside the organism
CA3070048A1 (en) * 2017-07-15 2019-01-24 Aleph Farms Ltd. Cultured meat compositions
CN107400633B (zh) * 2017-08-17 2023-10-10 上海白泽医疗器械有限公司 中空纤维交换器及中空纤维交换式培养系统
CN110117527A (zh) * 2019-05-15 2019-08-13 刘宝全 一种干细胞代谢废物的强化排出方法
CN110257227B (zh) * 2019-06-20 2020-08-04 江南大学 一种用于动物细胞培养的生物反应器
CN110201000A (zh) * 2019-07-11 2019-09-06 广东海洋大学 一种具有促进创伤愈合作用的方格星虫酶解蛋白肽粉的制备方法及其应用
CN110317848B (zh) * 2019-08-08 2020-02-18 北京工商大学 一种胶原蛋白肽的制备方法
EP4069824A1 (en) * 2019-12-02 2022-10-12 Avant Meats Company Limited Methods of meat production by in vitro cell cultivation

Also Published As

Publication number Publication date
ECSP22052002A (es) 2022-09-30
US20220025310A1 (en) 2022-01-27
PE20221183A1 (es) 2022-08-05
AU2022324741A1 (en) 2024-02-15
PE20221185A1 (es) 2022-08-05
BR112022010816A2 (pt) 2022-08-23
CO2022009121A2 (es) 2022-09-09
AU2020398072A1 (en) 2022-06-16
CA3173727A1 (en) 2021-06-10
KR20220110541A (ko) 2022-08-08
JP2023504217A (ja) 2023-02-01
PE20221274A1 (es) 2022-09-01
CA3163651A1 (en) 2021-06-10
EP4069861A4 (en) 2024-06-26
KR20240041372A (ko) 2024-03-29
AU2020396377A1 (en) 2022-06-16
IL293492A (en) 2022-08-01
EP4068987A1 (en) 2022-10-12
WO2021111270A1 (en) 2021-06-10
IL293495A (en) 2022-08-01
CA3163662A1 (en) 2021-06-10
PE20221184A1 (es) 2022-08-05
WO2021111263A1 (en) 2021-06-10
EP4069824A1 (en) 2022-10-12
ECSP22051770A (es) 2022-09-30
MX2022006593A (es) 2022-08-25
CA3163663A1 (en) 2021-06-10
BR112022010817A2 (pt) 2022-08-23
IL293494A (en) 2022-08-01
US20240093153A1 (en) 2024-03-21
EP4068987A4 (en) 2024-03-06
MX2022006591A (es) 2022-08-25
KR20220110798A (ko) 2022-08-09
CA3228282A1 (en) 2023-02-09
WO2021111219A1 (en) 2021-06-10
KR20220111310A (ko) 2022-08-09
CO2022009288A2 (es) 2022-09-09
CO2022009114A2 (es) 2022-09-09
BR112022010822A2 (pt) 2022-08-23
CN115443330A (zh) 2022-12-06
CN115298319A (zh) 2022-11-04
ECSP22051779A (es) 2022-09-30
KR20220110265A (ko) 2022-08-05
CN115443072A (zh) 2022-12-06
JP2023507242A (ja) 2023-02-21
CN117957304A (zh) 2024-04-30
EP4069861A1 (en) 2022-10-12
ECSP22051806A (es) 2022-09-30
IL293493A (en) 2022-08-01
EP4069821A4 (en) 2024-04-03
EP4381044A1 (en) 2024-06-12
CN115397969A (zh) 2022-11-25
JP2023504216A (ja) 2023-02-01
US20220298480A1 (en) 2022-09-22
MX2022006590A (es) 2022-09-19
AU2020396360A1 (en) 2022-06-16
TW202322704A (zh) 2023-06-16
WO2023012523A1 (en) 2023-02-09
JP2023504218A (ja) 2023-02-01
EP4069821A1 (en) 2022-10-12
MX2022006592A (es) 2022-08-25
AU2020397620A1 (en) 2022-06-16
CO2022009119A2 (es) 2022-09-09
WO2021111196A1 (en) 2021-06-10

Similar Documents

Publication Publication Date Title
BR112022010814A2 (pt) Métodos de produção de carne por cultivo de células in vitro
Seah et al. Scaffolds for the manufacture of cultured meat
Akhtar et al. Accelerated photoreceptor differentiation of hiPSC-derived retinal organoids by contact co-culture with retinal pigment epithelium
Shachar et al. Effects of mechanical stimulation induced by compression and medium perfusion on cardiac tissue engineering
BRPI0514641A (pt) cultivação das células tronco embriÈnica humana
WO2008120218A3 (en) Undifferentiated stem cell culture systems
Telfer Fertility preservation: progress and prospects for developing human immature oocytes in vitro
Song et al. Establishment of a corneal epithelial cell line spontaneously derived from human limbal cells
MX2022010521A (es) Metodo para producir linfocitos citoliticos naturales a partir de celulas madre pluripotentes.
PT2398898E (pt) Método para a criopreservação de células, construções celulares artificiais ou conjuntos de tecidos complexos tridimensionais
ATE525460T1 (de) Verfahren zur kultivierung und vermehrung hämatopoetischer stammzellen und vorläuferzellen unter verwendung menschlicher endometriumzellen
Reuter et al. Fact or fiction: In vitro spermatogenesis
ATE517176T1 (de) Verfahren zur kultivierung von geschmackszellen aus säugern
BR112022014305A2 (pt) Uso de homólogos de proteínas de plantas em meios de cultura
RU2019134339A (ru) Выделение клеток из вылупившихся яиц рептилий для применения для получения биоискусственной дермы и кожи для кожевенного производства
Zhang et al. Urothelial cell culture: stratified urothelial sheet and three-dimensional growth of urothelial structure
Egger et al. Physiologic isolation and expansion of human mesenchymal stem/stromal cells for manufacturing of cell‐based therapy products
Noor et al. Newer trends and techniques adopted for manufacturing of In vitro meat through “tissue-engineering” technology: a review
Sakai et al. Wrapping tissues with a pre-established cage-like layer composed of living cells
Zhang et al. Urothelial cell culture
CN106508891B (zh) 一种大理裂腹鱼肾脏细胞系的构建方法
CN103589637A (zh) 细胞体外立体培养系统
RU2012109240A (ru) Способ культивирования мышечных клеток in vitro для получения биомассы, используемой для производства мясных пищевых продуктов
KR102578904B1 (ko) 위 오가노이드 배양 및 이식을 위한 탈세포 위 조직 유래 지지체 및 이의 제조방법
BR112022024982A2 (pt) Processo e dispositivo para produção de carne de cultura