CO2022009121A2 - Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células - Google Patents

Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células

Info

Publication number
CO2022009121A2
CO2022009121A2 CONC2022/0009121A CO2022009121A CO2022009121A2 CO 2022009121 A2 CO2022009121 A2 CO 2022009121A2 CO 2022009121 A CO2022009121 A CO 2022009121A CO 2022009121 A2 CO2022009121 A2 CO 2022009121A2
Authority
CO
Colombia
Prior art keywords
fluid
unit configured
cell culture
cell
supply
Prior art date
Application number
CONC2022/0009121A
Other languages
English (en)
Inventor
Po San Mario Chin
Kai Yi Carrie Chan
Chuen Wai Li
Original Assignee
Avant Meats Company Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avant Meats Company Ltd filed Critical Avant Meats Company Ltd
Publication of CO2022009121A2 publication Critical patent/CO2022009121A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2311—Interleukin-11 (IL-11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Rheumatology (AREA)
  • Transplantation (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un sistema de cultivo celular que mejora la producción celular y reduce el costo del cultivo celular. El sistema mantiene una condición de cultivo óptima estabilizando el nivel de nutrientes y/o manteniendo un nivel mínimo de inhibidores de crecimiento en un medio de cultivo. El sistema comprende una unidad de cultivo celular configurada para retener al menos un tipo de célula; una unidad de medio fresco configurada para suministrar y recibir el primer fluido a la unidad de cultivo celular; una unidad de eliminación de desechos configurada para suministrar y recibir un segundo fluido de la unidad de medio fresco; y una unidad de diálisis configurada para extraer los desechos del primer fluido al segundo fluido y suministrar nutrientes desde el segundo fluido al primer fluido.
CONC2022/0009121A 2019-12-02 2022-06-30 Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células CO2022009121A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962942568P 2019-12-02 2019-12-02
PCT/IB2020/061257 WO2021111270A1 (en) 2019-12-02 2020-11-30 System for producing cultivated meats, tissues and associated products from cells

Publications (1)

Publication Number Publication Date
CO2022009121A2 true CO2022009121A2 (es) 2022-09-09

Family

ID=76221512

Family Applications (4)

Application Number Title Priority Date Filing Date
CONC2022/0009114A CO2022009114A2 (es) 2019-12-02 2022-06-30 Métodos de producción de carne por cultivo celular in vitro
CONC2022/0009119A CO2022009119A2 (es) 2019-12-02 2022-06-30 Métodos para mejorar el cultivo celular con proteínas específicas de cada especie o específicas de cada género y las aplicaciones de los mismos
CONC2022/0009121A CO2022009121A2 (es) 2019-12-02 2022-06-30 Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células
CONC2022/0009288A CO2022009288A2 (es) 2019-12-02 2022-07-01 Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CONC2022/0009114A CO2022009114A2 (es) 2019-12-02 2022-06-30 Métodos de producción de carne por cultivo celular in vitro
CONC2022/0009119A CO2022009119A2 (es) 2019-12-02 2022-06-30 Métodos para mejorar el cultivo celular con proteínas específicas de cada especie o específicas de cada género y las aplicaciones de los mismos

Family Applications After (1)

Application Number Title Priority Date Filing Date
CONC2022/0009288A CO2022009288A2 (es) 2019-12-02 2022-07-01 Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos

Country Status (15)

Country Link
US (3) US20240093153A1 (es)
EP (5) EP4069824A1 (es)
JP (4) JP2023507242A (es)
KR (5) KR20220110798A (es)
CN (5) CN115443330A (es)
AU (5) AU2020396360A1 (es)
BR (4) BR112022010814A2 (es)
CA (5) CA3163651A1 (es)
CO (4) CO2022009114A2 (es)
EC (4) ECSP22051770A (es)
IL (4) IL293492A (es)
MX (4) MX2022006590A (es)
PE (4) PE20221184A1 (es)
TW (1) TW202322704A (es)
WO (5) WO2021111196A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4069824A1 (en) * 2019-12-02 2022-10-12 Avant Meats Company Limited Methods of meat production by in vitro cell cultivation
CN114181888B (zh) * 2021-11-24 2023-12-08 汕头大学 一种浅色黄姑鱼鱼鳔细胞系的建立方法及应用
WO2023233340A1 (en) * 2022-06-02 2023-12-07 Avant Meats Company Limited Genetically engineered yeast cells, recombinant proteins produced thereof and applications thereof
WO2024028439A1 (en) 2022-08-03 2024-02-08 Novelty For Them Gmbh Complexed asthaxanthin undergoing a hypsochromic shift upon heating and application in food products

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3418208A (en) * 1966-02-28 1968-12-24 Mobil Oil Corp Growing increased yields of micro-organisms
FR2594847B1 (fr) * 1986-02-25 1989-06-02 Moet Hennessy Rech Procede de preparation de polypeptides biologiquement actifs, polypeptides obtenus et compositions les contenant
DK0615545T3 (da) * 1991-11-20 2000-10-30 Innogenetics Nv Lysater hidrørende fra keratinocytter til brug som sårhelingsmidler
WO1999031222A1 (en) * 1997-12-18 1999-06-24 Willem Frederik Van Eelen Industrial scale production of meat from in vitro cell cultures
US6664104B2 (en) * 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
US20070122904A1 (en) * 2000-09-29 2007-05-31 Unisearch Limited Method and apparatus for culturing cells
AU2002361549A1 (en) * 2001-07-13 2003-04-14 Paul L. Deangelis Glycosaminoglycan polymers and methods of making and using same
AU2003298319A1 (en) * 2002-12-03 2004-06-23 Celltran Limited Pharmaceutical compositions of cell lysate and processes for the production and use thereof
US20060153797A1 (en) * 2004-06-11 2006-07-13 Bortolotto Susan K Tissue material and matrix
AU2004324099B2 (en) * 2004-09-17 2011-02-03 Jon Vein Tissue engineered meat for consumption and a method for producing tissue engineered meat for consumption
WO2007139748A2 (en) * 2006-05-22 2007-12-06 Biovest International Inc. Extra-capillary fluid cycling system and method for a cell culture device
US20080254167A1 (en) * 2007-04-10 2008-10-16 Solae, Llc Seafood Compositions Comprising Structured Protein Products
CA2742583C (en) * 2008-11-04 2022-09-27 Viacyte, Inc. Stem cell aggregate suspension compositions and methods for differentiation thereof
US20130323708A1 (en) * 2009-07-01 2013-12-05 Massachusetts Institute Of Technology Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same
WO2011103359A2 (en) * 2010-02-17 2011-08-25 Inq Biosciences Corporation Culture systems, apparatus, and related methods and articles
WO2012103922A1 (en) * 2011-01-31 2012-08-09 Red Patent B.V. Installation and method for biomass conversion into methane
EP2885415B1 (en) * 2012-08-17 2022-08-03 Easel Biotechnologies LLC Two stage production of higher alcohols
CN105899079A (zh) * 2013-10-30 2016-08-24 密苏里大学的策展人 可扩展性骨骼肌谱系特化和培育的方法
CN104109705B (zh) * 2014-06-19 2017-10-31 广东省农业科学院蚕业与农产品加工研究所 一种酶法制备具有促进伤口愈合活性的海洋胶原蛋白肽的方法
US20180030398A1 (en) * 2015-02-09 2018-02-01 Univercells Nv System, apparatus and method for the production of cells and/or cell products
CN109714962A (zh) * 2016-07-11 2019-05-03 耶路撒冷希伯来大学的益生研究开发有限公司 用于在体外培养细胞的系统和方法
MX2019000881A (es) * 2016-07-28 2019-07-01 Fonterra Cooperative Group Ltd Producto lacteo y proceso.
CN106749634B (zh) * 2017-01-17 2020-09-15 北京鸿顺养源生物科技有限公司 一种具有抗氧化活性的猪血红蛋白糖肽及其制备方法与应用
CN106805252A (zh) * 2017-01-22 2017-06-09 福州大学 一种鱼短肽及其在营养强化剂中的应用
IL308692A (en) * 2017-06-07 2024-01-01 Wild Type Inc Meat production outside the organism
CA3070048A1 (en) * 2017-07-15 2019-01-24 Aleph Farms Ltd. Cultured meat compositions
CN107400633B (zh) * 2017-08-17 2023-10-10 上海白泽医疗器械有限公司 中空纤维交换器及中空纤维交换式培养系统
CN110117527A (zh) * 2019-05-15 2019-08-13 刘宝全 一种干细胞代谢废物的强化排出方法
CN110257227B (zh) * 2019-06-20 2020-08-04 江南大学 一种用于动物细胞培养的生物反应器
CN110201000A (zh) * 2019-07-11 2019-09-06 广东海洋大学 一种具有促进创伤愈合作用的方格星虫酶解蛋白肽粉的制备方法及其应用
CN110317848B (zh) * 2019-08-08 2020-02-18 北京工商大学 一种胶原蛋白肽的制备方法
EP4069824A1 (en) * 2019-12-02 2022-10-12 Avant Meats Company Limited Methods of meat production by in vitro cell cultivation

Also Published As

Publication number Publication date
ECSP22052002A (es) 2022-09-30
US20220025310A1 (en) 2022-01-27
PE20221183A1 (es) 2022-08-05
AU2022324741A1 (en) 2024-02-15
PE20221185A1 (es) 2022-08-05
BR112022010816A2 (pt) 2022-08-23
AU2020398072A1 (en) 2022-06-16
CA3173727A1 (en) 2021-06-10
KR20220110541A (ko) 2022-08-08
JP2023504217A (ja) 2023-02-01
PE20221274A1 (es) 2022-09-01
CA3163651A1 (en) 2021-06-10
EP4069861A4 (en) 2024-06-26
BR112022010814A2 (pt) 2022-08-23
KR20240041372A (ko) 2024-03-29
AU2020396377A1 (en) 2022-06-16
IL293492A (en) 2022-08-01
EP4068987A1 (en) 2022-10-12
WO2021111270A1 (en) 2021-06-10
IL293495A (en) 2022-08-01
CA3163662A1 (en) 2021-06-10
PE20221184A1 (es) 2022-08-05
WO2021111263A1 (en) 2021-06-10
EP4069824A1 (en) 2022-10-12
ECSP22051770A (es) 2022-09-30
MX2022006593A (es) 2022-08-25
CA3163663A1 (en) 2021-06-10
BR112022010817A2 (pt) 2022-08-23
IL293494A (en) 2022-08-01
US20240093153A1 (en) 2024-03-21
EP4068987A4 (en) 2024-03-06
MX2022006591A (es) 2022-08-25
KR20220110798A (ko) 2022-08-09
CA3228282A1 (en) 2023-02-09
WO2021111219A1 (en) 2021-06-10
KR20220111310A (ko) 2022-08-09
CO2022009288A2 (es) 2022-09-09
CO2022009114A2 (es) 2022-09-09
BR112022010822A2 (pt) 2022-08-23
CN115443330A (zh) 2022-12-06
CN115298319A (zh) 2022-11-04
ECSP22051779A (es) 2022-09-30
KR20220110265A (ko) 2022-08-05
CN115443072A (zh) 2022-12-06
JP2023507242A (ja) 2023-02-21
CN117957304A (zh) 2024-04-30
EP4069861A1 (en) 2022-10-12
ECSP22051806A (es) 2022-09-30
IL293493A (en) 2022-08-01
EP4069821A4 (en) 2024-04-03
EP4381044A1 (en) 2024-06-12
CN115397969A (zh) 2022-11-25
JP2023504216A (ja) 2023-02-01
US20220298480A1 (en) 2022-09-22
MX2022006590A (es) 2022-09-19
AU2020396360A1 (en) 2022-06-16
TW202322704A (zh) 2023-06-16
WO2023012523A1 (en) 2023-02-09
JP2023504218A (ja) 2023-02-01
EP4069821A1 (en) 2022-10-12
MX2022006592A (es) 2022-08-25
AU2020397620A1 (en) 2022-06-16
CO2022009119A2 (es) 2022-09-09
WO2021111196A1 (en) 2021-06-10

Similar Documents

Publication Publication Date Title
ECSP22051806A (es) Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células
BR112017007770A2 (pt) cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.
MX2021010518A (es) Medios y metodos de fermentacion para producir polisacarido en cultivo celular bacteriano.
EP4148121A3 (en) Cell-controlled perfusion in continuous culture
WO2015157555A3 (en) Import of unnatural or modified nucleoside triphosphates into cells via nucleic acid triphosphate transporters
CL2011001926A1 (es) Biorreactor para el cultivo de celulas de mamiferos donde dicho biorreactor posee un volumen de al menos 4000 l y al menos un impulsor superior y al menos un impulsor de fondo, donde el impulsor superior es un impulsor de hidroplano; metodo de cultivo; sistema de bioreactor.
AR093460A1 (es) Medios de cultivo celular
EA201000020A1 (ru) Способы обработки сред для культур клеток, применяемых в биореакторах
BR112013020217B1 (pt) método para cultivar de organismos em um fotobiorreator tubular
AR095354A1 (es) Métodos para el armado de bancos celulares de alta densidad
BR112012016437A2 (pt) fotobiorreator em meio fechado para a cultura de micro-organismos fotossintéticos.
EA201491785A1 (ru) Способ ферментации
BR112013028544A2 (pt) método para produzir um composto derivado de acetil-coa heterólogo
AR114504A1 (es) Regeneración de plantas genéticamente modificadas
MX2017002234A (es) Metodos de cultivo semicontinuo.
AR092630A1 (es) Metodos y composiciones para prevenir la incorporacion erronea de norleucina en proteinas
BR112017004017A2 (pt) sistema de fermentação para processos de moagem seca
BR112018074315A2 (pt) método para produzir óleo com ácidos graxos saturados reduzidos.
BR112014002980A2 (pt) método de produção de álcool isopropílico por cultivo contínuo
CY1111008T1 (el) Προϊον με ζωντανους προβιοτικους μικροοργανισμους
BR112017000142A2 (pt) ajuste de ph para melhorar recuperação de criopreservação de bancos de células
BR112018017340A2 (pt) método para produzir um aminoácido básico ou um produto de fermentação contendo o aminoácido básico
WO2015177641A3 (en) Methods of oil production in microorganisms
AR122838A1 (es) Electrodiálisis de sales de aminoácidos en celdas de tres compartimientos con membrana bipolar
AR106016A1 (es) Composición fertilizante que incluye un inhibidor de la actividad ureasa