ECSP22051770A - Métodos de producción de carne por cultivo celular in vitro - Google Patents

Métodos de producción de carne por cultivo celular in vitro

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Publication number
ECSP22051770A
ECSP22051770A ECSENADI202251770A ECDI202251770A ECSP22051770A EC SP22051770 A ECSP22051770 A EC SP22051770A EC SENADI202251770 A ECSENADI202251770 A EC SENADI202251770A EC DI202251770 A ECDI202251770 A EC DI202251770A EC SP22051770 A ECSP22051770 A EC SP22051770A
Authority
EC
Ecuador
Prior art keywords
cells
growing
vitro cell
cell culture
animal
Prior art date
Application number
ECSENADI202251770A
Other languages
English (en)
Inventor
Po San Mario Chin
Kai Yi Carrie Chan
Chun Hei Poon
Original Assignee
Avant Meats Company Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avant Meats Company Ltd filed Critical Avant Meats Company Ltd
Publication of ECSP22051770A publication Critical patent/ECSP22051770A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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Abstract

Un método para la producción de carne mediante el cultivo de células in vitro incluye el aislamiento de tejido de una fuente animal o vegetal y la fabricación de una suspensión celular de células, y el crecimiento de las células en una estructura sólida o semisólida que imita un órgano animal mediante el crecimiento de las células en un andamiaje de calidad alimentaria en un medio de cultivo. La expresión de una o más proteínas en las células en crecimiento se puede aumentar mediante la alteración de un nivel de uno o más microARN que regulan la expresión de la proteína. Además, las células en crecimiento se pueden co-cultivar con células de bioingeniería que secretan factores de crecimiento y citocinas que apoyan el crecimiento celular in situ. La técnica de co-cultivo reduce o elimina la necesidad de suero fetal bovino de origen animal en el medio de cultivo.
ECSENADI202251770A 2019-12-02 2022-07-01 Métodos de producción de carne por cultivo celular in vitro ECSP22051770A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201962942568P 2019-12-02 2019-12-02

Publications (1)

Publication Number Publication Date
ECSP22051770A true ECSP22051770A (es) 2022-09-30

Family

ID=76221512

Family Applications (4)

Application Number Title Priority Date Filing Date
ECSENADI202251779A ECSP22051779A (es) 2019-12-02 2022-07-01 Métodos para mejorar el cultivo celular con proteínas específicas de cada especie o específicas de cada género y las aplicaciones de los mismos
ECSENADI202252002A ECSP22052002A (es) 2019-12-02 2022-07-01 Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos
ECSENADI202251806A ECSP22051806A (es) 2019-12-02 2022-07-01 Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células
ECSENADI202251770A ECSP22051770A (es) 2019-12-02 2022-07-01 Métodos de producción de carne por cultivo celular in vitro

Family Applications Before (3)

Application Number Title Priority Date Filing Date
ECSENADI202251779A ECSP22051779A (es) 2019-12-02 2022-07-01 Métodos para mejorar el cultivo celular con proteínas específicas de cada especie o específicas de cada género y las aplicaciones de los mismos
ECSENADI202252002A ECSP22052002A (es) 2019-12-02 2022-07-01 Métodos de cultivo de células, preparación de hidrolizado a partir de células y aplicaciones de los mismos
ECSENADI202251806A ECSP22051806A (es) 2019-12-02 2022-07-01 Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de células

Country Status (15)

Country Link
US (3) US20240093153A1 (es)
EP (5) EP4069824A1 (es)
JP (4) JP2023507242A (es)
KR (5) KR20220110798A (es)
CN (5) CN115443330A (es)
AU (5) AU2020396360A1 (es)
BR (4) BR112022010814A2 (es)
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Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3163651A1 (en) * 2019-12-02 2021-06-10 Avant Meats Company Limited Methods of meat production by in vitro cell cultivation
CN114181888B (zh) * 2021-11-24 2023-12-08 汕头大学 一种浅色黄姑鱼鱼鳔细胞系的建立方法及应用
WO2023233340A1 (en) * 2022-06-02 2023-12-07 Avant Meats Company Limited Genetically engineered yeast cells, recombinant proteins produced thereof and applications thereof
WO2024028439A1 (en) 2022-08-03 2024-02-08 Novelty For Them Gmbh Complexed asthaxanthin undergoing a hypsochromic shift upon heating and application in food products

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3418208A (en) * 1966-02-28 1968-12-24 Mobil Oil Corp Growing increased yields of micro-organisms
FR2594847B1 (fr) * 1986-02-25 1989-06-02 Moet Hennessy Rech Procede de preparation de polypeptides biologiquement actifs, polypeptides obtenus et compositions les contenant
JP3311351B2 (ja) * 1991-11-20 2002-08-05 エヌ・ブイ・インノジェネティクス・ソシエテ・アノニム 新規なケラチノサイト培養物，その調製方法及び創傷治療物質としてのその用途
WO1999031222A1 (en) * 1997-12-18 1999-06-24 Willem Frederik Van Eelen Industrial scale production of meat from in vitro cell cultures
US6664104B2 (en) * 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
US20070122904A1 (en) * 2000-09-29 2007-05-31 Unisearch Limited Method and apparatus for culturing cells
AU2002361549A1 (en) * 2001-07-13 2003-04-14 Paul L. Deangelis Glycosaminoglycan polymers and methods of making and using same
CN100369630C (zh) * 2002-12-03 2008-02-20 细胞移种有限公司 细胞裂解物的药用组合物及其生产和使用方法
US20060153797A1 (en) * 2004-06-11 2006-07-13 Bortolotto Susan K Tissue material and matrix
ES2378822T3 (es) * 2004-09-17 2012-04-18 Jon Vein Carne de ingeniería tisular para el consumo y método para producir carne de ingeniería tisular para el consumo
WO2007139742A1 (en) * 2006-05-22 2007-12-06 Biovest International Inc. Method and system for the production of cells and cell products and applications thereof
US20080254167A1 (en) * 2007-04-10 2008-10-16 Solae, Llc Seafood Compositions Comprising Structured Protein Products
JP5390624B2 (ja) * 2008-11-04 2014-01-15 バイアサイト インク 幹細胞集合体懸濁液組成物、その分化方法
US20130323708A1 (en) * 2009-07-01 2013-12-05 Massachusetts Institute Of Technology Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same
WO2011103359A2 (en) * 2010-02-17 2011-08-25 Inq Biosciences Corporation Culture systems, apparatus, and related methods and articles
WO2012103922A1 (en) * 2011-01-31 2012-08-09 Red Patent B.V. Installation and method for biomass conversion into methane
US10233467B2 (en) * 2012-08-17 2019-03-19 Easel Biotechnologies, Llc Two-stage production of higher alcohols
JP6728049B2 (ja) * 2013-10-30 2020-07-22 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ 拡張性のある骨格筋系譜形成及び培養の方法
CN104109705B (zh) * 2014-06-19 2017-10-31 广东省农业科学院蚕业与农产品加工研究所 一种酶法制备具有促进伤口愈合活性的海洋胶原蛋白肽的方法
AU2016218037B2 (en) * 2015-02-09 2020-12-17 Univercells Technologies S.A. System, apparatus and method for the production of cells and/or cell products
EP3481191A2 (en) * 2016-07-11 2019-05-15 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Systems and methods for growing cells in vitro
MX2019000881A (es) * 2016-07-28 2019-07-01 Fonterra Cooperative Group Ltd Producto lacteo y proceso.
CN106749634B (zh) * 2017-01-17 2020-09-15 北京鸿顺养源生物科技有限公司 一种具有抗氧化活性的猪血红蛋白糖肽及其制备方法与应用
CN106805252A (zh) * 2017-01-22 2017-06-09 福州大学 一种鱼短肽及其在营养强化剂中的应用
WO2018227016A1 (en) * 2017-06-07 2018-12-13 Wild Type, Inc. Ex vivo meat production
AU2018304618A1 (en) * 2017-07-15 2020-03-05 Aleph Farms Ltd Cultured meat compositions
CN107400633B (zh) * 2017-08-17 2023-10-10 上海白泽医疗器械有限公司 中空纤维交换器及中空纤维交换式培养系统
CN110117527A (zh) * 2019-05-15 2019-08-13 刘宝全 一种干细胞代谢废物的强化排出方法
CN110257227B (zh) * 2019-06-20 2020-08-04 江南大学 一种用于动物细胞培养的生物反应器
CN110201000A (zh) * 2019-07-11 2019-09-06 广东海洋大学 一种具有促进创伤愈合作用的方格星虫酶解蛋白肽粉的制备方法及其应用
CN110317848B (zh) * 2019-08-08 2020-02-18 北京工商大学 一种胶原蛋白肽的制备方法
CA3163651A1 (en) * 2019-12-02 2021-06-10 Avant Meats Company Limited Methods of meat production by in vitro cell cultivation

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