PE20221184A1 - Metodos de produccion de carne por cultivo celular in vitro - Google Patents
Metodos de produccion de carne por cultivo celular in vitroInfo
- Publication number
- PE20221184A1 PE20221184A1 PE2022001001A PE2022001001A PE20221184A1 PE 20221184 A1 PE20221184 A1 PE 20221184A1 PE 2022001001 A PE2022001001 A PE 2022001001A PE 2022001001 A PE2022001001 A PE 2022001001A PE 20221184 A1 PE20221184 A1 PE 20221184A1
- Authority
- PE
- Peru
- Prior art keywords
- cells
- growing
- animal
- vitro cell
- cell culture
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000012606 in vitro cell culture Methods 0.000 title abstract 2
- 235000013372 meat Nutrition 0.000 title abstract 2
- 241001465754 Metazoa Species 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 1
- 102000004127 Cytokines Human genes 0.000 abstract 1
- 108090000695 Cytokines Proteins 0.000 abstract 1
- 108700011259 MicroRNAs Proteins 0.000 abstract 1
- 210000001557 animal structure Anatomy 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 239000006285 cell suspension Substances 0.000 abstract 1
- 238000003501 co-culture Methods 0.000 abstract 1
- 239000012091 fetal bovine serum Substances 0.000 abstract 1
- 239000003102 growth factor Substances 0.000 abstract 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0679—Cells of the gastro-intestinal tract
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
- C12N2310/141—MicroRNAs, miRNAs
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2306—Interleukin-6 (IL-6)
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Abstract
Un metodo para la produccion de carne mediante el cultivo de celulas in vitro incluye el aislamiento de tejido de una fuente animal o vegetal y la fabricacion de una suspension celular de celulas, y el crecimiento de las celulas en una estructura solida o semisolida que imita un organo animal mediante el crecimiento de las celulas en un andamiaje de calidad alimentaria en un medio de cultivo. La expresion de una o mas proteinas en las celulas en crecimiento se puede aumentar mediante la alteracion de un nivel de uno o mas microARN que regulan la expresion de la proteina. Ademas, las celulas en crecimiento se pueden co-cultivar con celulas de bioingenieria que secretan factores de crecimiento y citocinas que apoyan el crecimiento celular in situ. La tecnica de co-cultivo reduce o elimina la necesidad de suero fetal bovino de origen animal en el medio de cultivo
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962942568P | 2019-12-02 | 2019-12-02 | |
PCT/IB2020/057119 WO2021111196A1 (en) | 2019-12-02 | 2020-07-28 | Methods of meat production by in vitro cell cultivation |
Publications (1)
Publication Number | Publication Date |
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PE20221184A1 true PE20221184A1 (es) | 2022-08-05 |
Family
ID=76221512
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PE2022001001A PE20221184A1 (es) | 2019-12-02 | 2020-07-28 | Metodos de produccion de carne por cultivo celular in vitro |
PE2022001000A PE20221183A1 (es) | 2019-12-02 | 2020-11-14 | Metodos de cultivo de celulas, preparacion de hidrolizado a partir de celulas y aplicaciones de los mismos |
PE2022001005A PE20221185A1 (es) | 2019-12-02 | 2020-11-27 | Metodos para mejorar el crecimiento celular con proteinas especificas de especie o genero y sus aplicaciones |
PE2022001006A PE20221274A1 (es) | 2019-12-02 | 2020-11-30 | Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de celulas |
Family Applications After (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PE2022001000A PE20221183A1 (es) | 2019-12-02 | 2020-11-14 | Metodos de cultivo de celulas, preparacion de hidrolizado a partir de celulas y aplicaciones de los mismos |
PE2022001005A PE20221185A1 (es) | 2019-12-02 | 2020-11-27 | Metodos para mejorar el crecimiento celular con proteinas especificas de especie o genero y sus aplicaciones |
PE2022001006A PE20221274A1 (es) | 2019-12-02 | 2020-11-30 | Sistema para producir carnes cultivadas, tejidos y productos asociados a partir de celulas |
Country Status (15)
Country | Link |
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US (3) | US20240093153A1 (es) |
EP (5) | EP4069824A1 (es) |
JP (5) | JP2023507242A (es) |
KR (5) | KR20220110798A (es) |
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WO2024134658A1 (en) * | 2022-12-22 | 2024-06-27 | Sea2Cell Ltd. | Compositions and methods for culturing cells of aquatic species |
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