BRPI0514641A - cultivação das células tronco embriÈnica humana - Google Patents

cultivação das células tronco embriÈnica humana

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Publication number
BRPI0514641A
BRPI0514641A BRPI0514641-0A BRPI0514641A BRPI0514641A BR PI0514641 A BRPI0514641 A BR PI0514641A BR PI0514641 A BRPI0514641 A BR PI0514641A BR PI0514641 A BRPI0514641 A BR PI0514641A
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BR
Brazil
Prior art keywords
cells
stem cells
human embryonic
embryonic stem
medium
Prior art date
Application number
BRPI0514641-0A
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English (en)
Inventor
James A Thomson
Tenneille Ludwig
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of BRPI0514641A publication Critical patent/BRPI0514641A/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
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  • Health & Medical Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

CULTIVAçãO DAS CéLULAS TRONCO EMBRIÈNICA HUMANA Os métodos prévios para cultivação das células tronco embriónica humana tem requerido ou células alimentadoras do fibroblasto ou um meio, que tem sido exposto às células alimentadoras do fibroblasto, a fim de manter as células tronco em um estado não diferenciado. Agora tem agora sido constatado que se os níveis elevados do fator de crescimento do fibroblasto são empregados em um meio com ácido gama amino butírico, ácido pipecólico, lítio e lipídios, as células tronco permanecerão não diferenciadas indefinidamente através de múltiplas passagens, ainda sem células alimentadoras ou meio condicionado. Uma matriz humanizada de proteínas humanas pode ser empregada como uma matriz basal para cultivar as células. As novas linhas de células tronco embriónica humana produzem empregando estas condições de cultivo, o meio e a matriz, nunca terá sido exposta a células animais, produtos animais, células alimentadoras ou meio condicionado.
BRPI0514641-0A 2004-09-08 2005-09-08 cultivação das células tronco embriÈnica humana BRPI0514641A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US60804004P 2004-09-08 2004-09-08
US69510005P 2005-06-29 2005-06-29
PCT/US2005/031839 WO2006029198A2 (en) 2004-09-08 2005-09-08 Culturing human embryonic stem cells

Publications (1)

Publication Number Publication Date
BRPI0514641A true BRPI0514641A (pt) 2008-06-17

Family

ID=35809711

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0514641-0A BRPI0514641A (pt) 2004-09-08 2005-09-08 cultivação das células tronco embriÈnica humana

Country Status (16)

Country Link
US (2) US7442548B2 (pt)
EP (2) EP3196296B1 (pt)
JP (1) JP2008518585A (pt)
KR (1) KR20070058584A (pt)
AU (1) AU2005282414C1 (pt)
BR (1) BRPI0514641A (pt)
CA (1) CA2579652A1 (pt)
DK (1) DK3196296T3 (pt)
ES (2) ES2708384T3 (pt)
GB (1) GB2432835B (pt)
IL (1) IL181438A (pt)
IS (1) IS8619A (pt)
MX (1) MX2007002389A (pt)
NO (1) NO344696B1 (pt)
NZ (1) NZ553235A (pt)
WO (1) WO2006029198A2 (pt)

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