NO20071486L - Dyrking av humane embryonale stamceller - Google Patents
Dyrking av humane embryonale stamcellerInfo
- Publication number
- NO20071486L NO20071486L NO20071486A NO20071486A NO20071486L NO 20071486 L NO20071486 L NO 20071486L NO 20071486 A NO20071486 A NO 20071486A NO 20071486 A NO20071486 A NO 20071486A NO 20071486 L NO20071486 L NO 20071486L
- Authority
- NO
- Norway
- Prior art keywords
- cells
- stem cells
- medium
- human embryonic
- embryonic stem
- Prior art date
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Abstract
Tidligere fremgangsmåter for dyrkning av humane embryonale stamceller har krevd enten fibroblast næringsceller eller et medium som er blitt eksponert for fibroblast næringsceller for å opprettholde stamcellene i en udifferensiert tilstand. Det er nå fiurmet at dersom høye nivåer av fibroblastvekstfaktor anvendes i et medium med gammaaminosmørsyre, pipecholinsyre, litium og lipider, vil stamcellene opprettholdes uendelig i udifferensiert tilstand gjermom mange passasjer, selv uten næringsceller eller kondisjonert medium. En humanisert matriks av humane proteiner kan anvendes som en grunnmatriks for å dyrke cellene. Nye linjer av humane embryonale stamceller er laget ved hjelp av disse kulturbetingelsene, der mediet og matriksen aldri er blitt eksponert for dyreceller, dyreproteiner, næringsceller eller kondisjonert medium. Effekt RV veldtlUdDr på ccUeprolffcreiiiig og dtfrcramlering
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US60804004P | 2004-09-08 | 2004-09-08 | |
US69510005P | 2005-06-29 | 2005-06-29 | |
PCT/US2005/031839 WO2006029198A2 (en) | 2004-09-08 | 2005-09-08 | Culturing human embryonic stem cells |
Publications (2)
Publication Number | Publication Date |
---|---|
NO20071486L true NO20071486L (no) | 2007-03-21 |
NO344696B1 NO344696B1 (no) | 2020-03-09 |
Family
ID=35809711
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO20071486A NO344696B1 (no) | 2004-09-08 | 2007-03-21 | Cellekulturmedium egnet for å opprettholde stamceller i en udifferensiert tilstand gjennom mange dyrkningspassasjer. |
Country Status (16)
Country | Link |
---|---|
US (2) | US7442548B2 (no) |
EP (2) | EP1797172B1 (no) |
JP (1) | JP2008518585A (no) |
KR (1) | KR20070058584A (no) |
AU (1) | AU2005282414C1 (no) |
BR (1) | BRPI0514641A (no) |
CA (1) | CA2579652A1 (no) |
DK (1) | DK3196296T3 (no) |
ES (2) | ES2708384T3 (no) |
GB (1) | GB2432835B (no) |
IL (1) | IL181438A (no) |
IS (1) | IS8619A (no) |
MX (1) | MX2007002389A (no) |
NO (1) | NO344696B1 (no) |
NZ (1) | NZ553235A (no) |
WO (1) | WO2006029198A2 (no) |
Families Citing this family (113)
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ES2708384T3 (es) * | 2004-09-08 | 2019-04-09 | Wisconsin Alumni Res Found | Cultivo de células madre embrionarias humanas |
CN101052712A (zh) * | 2004-09-08 | 2007-10-10 | 威斯康星校友研究基金会 | 培养人胚胎干细胞 |
US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
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US7410798B2 (en) * | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
US20030232430A1 (en) * | 2001-11-26 | 2003-12-18 | Advanced Cell Technology | Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells |
HUE028026T2 (en) * | 2002-12-16 | 2016-11-28 | Technion Res & Dev Foundation | Breeding system free of native cells, free of foreign matter for human embryonic stem cells |
KR100632851B1 (ko) * | 2004-01-20 | 2006-10-13 | 한국원자력연구소 | 전분화능 줄기세포의 분화를 억제하는 방법 |
ES2708384T3 (es) * | 2004-09-08 | 2019-04-09 | Wisconsin Alumni Res Found | Cultivo de células madre embrionarias humanas |
CN101052712A (zh) * | 2004-09-08 | 2007-10-10 | 威斯康星校友研究基金会 | 培养人胚胎干细胞 |
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GB2432835A (en) | 2007-06-06 |
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GB0706625D0 (en) | 2007-05-16 |
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MX2007002389A (es) | 2009-02-12 |
US7442548B2 (en) | 2008-10-28 |
EP3196296A1 (en) | 2017-07-26 |
ES2708384T3 (es) | 2019-04-09 |
DK3196296T3 (en) | 2019-02-04 |
WO2006029198A3 (en) | 2006-05-18 |
EP1797172B1 (en) | 2017-04-19 |
GB2432835B (en) | 2009-04-08 |
AU2005282414B2 (en) | 2010-12-02 |
NO344696B1 (no) | 2020-03-09 |
AU2005282414C1 (en) | 2011-04-07 |
CA2579652A1 (en) | 2006-03-16 |
WO2006029198A2 (en) | 2006-03-16 |
NZ553235A (en) | 2009-11-27 |
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