MX2020002851A - Biorreactor de perfusion y metodos de uso relacionados. - Google Patents

Biorreactor de perfusion y metodos de uso relacionados.

Info

Publication number
MX2020002851A
MX2020002851A MX2020002851A MX2020002851A MX2020002851A MX 2020002851 A MX2020002851 A MX 2020002851A MX 2020002851 A MX2020002851 A MX 2020002851A MX 2020002851 A MX2020002851 A MX 2020002851A MX 2020002851 A MX2020002851 A MX 2020002851A
Authority
MX
Mexico
Prior art keywords
cell culture
bioreactor
cell
specified rate
culture
Prior art date
Application number
MX2020002851A
Other languages
English (en)
Inventor
Matthew Angelini
Ashley Witmer
Anthony Debiase
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=64110133&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2020002851(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2020002851A publication Critical patent/MX2020002851A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un método para controlar un sistema de biorreactor incluye proporcionar un cultivo celular en un biorreactor, en donde las condiciones en el biorreactor permiten que el cultivo celular produzca una proteína de interés (POI), medir parámetros del proceso (PP) del cultivo dentro del biorreactor mediante RAMAN, en donde los parámetros del proceso son seleccionados del grupo que consiste en concentración de nutrientes, concentración de células viables y atributos de la proteína, medir un peso predeterminado del biorreactor con el cultivo celular, extraer el medio agotado libre de células del cultivo celular empleando un primer conducto de salida a una primera tasa predeterminada, extraer las células del cultivo celular empleando un segundo conducto de salida a una segunda tasa predeterminada e introducir uno de medio fresco o nutrientes, o ambos, en el cultivo celular empleando un conducto de entrada a una tercera tasa predeterminada.
MX2020002851A 2017-10-16 2018-10-15 Biorreactor de perfusion y metodos de uso relacionados. MX2020002851A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762572918P 2017-10-16 2017-10-16
PCT/US2018/055891 WO2019079188A1 (en) 2017-10-16 2018-10-15 PERFUSION BIOREACTOR AND METHODS OF USE THEREOF

Publications (1)

Publication Number Publication Date
MX2020002851A true MX2020002851A (es) 2020-07-24

Family

ID=64110133

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020002851A MX2020002851A (es) 2017-10-16 2018-10-15 Biorreactor de perfusion y metodos de uso relacionados.

Country Status (18)

Country Link
US (2) US11193103B2 (es)
EP (2) EP3697887B1 (es)
JP (2) JP7337784B2 (es)
KR (1) KR20200062236A (es)
CN (1) CN111212899A (es)
AR (1) AR113772A1 (es)
AU (1) AU2018350898A1 (es)
CA (1) CA3075679A1 (es)
DK (1) DK3697887T3 (es)
EA (1) EA202090740A1 (es)
ES (1) ES2919850T3 (es)
HU (1) HUE058924T2 (es)
IL (1) IL273150A (es)
MX (1) MX2020002851A (es)
PL (1) PL3697887T3 (es)
SG (1) SG11202002339XA (es)
TW (1) TWI827555B (es)
WO (1) WO2019079188A1 (es)

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US11774287B2 (en) 2019-05-28 2023-10-03 WuXi Biologics Ireland Limited Raman spectroscopy integrated perfusion cell culture system for monitoring and auto-controlling perfusion cell culture
TW202045711A (zh) * 2019-06-13 2020-12-16 美商安進公司 生物製品製造中基於生物量之自動灌注控制
US20220380712A1 (en) * 2019-11-07 2022-12-01 Merck Patent Gmbh Methods and systems for performing perfusion cell culture
WO2021136798A1 (en) 2020-01-02 2021-07-08 F. Hoffmann-La Roche Ag Single-use cell culture container with one or more in-situ online sensors
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US20230323264A1 (en) * 2022-04-07 2023-10-12 Mirror Biologics, Inc. Artificial lymph node bioreactor
JP2024030410A (ja) 2022-08-24 2024-03-07 横河電機株式会社 細胞培養システム、及び細胞培養方法
NL2033094B1 (en) * 2022-09-21 2024-03-26 Simabs Nv Method for controlling a perfusion process in a bioreactor

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Also Published As

Publication number Publication date
DK3697887T3 (da) 2022-05-02
BR112020005209A2 (pt) 2020-09-15
US11965153B2 (en) 2024-04-23
KR20200062236A (ko) 2020-06-03
CA3075679A1 (en) 2019-04-25
PL3697887T3 (pl) 2022-08-01
US20190153381A1 (en) 2019-05-23
TW201934744A (zh) 2019-09-01
EP3697887A1 (en) 2020-08-26
TWI827555B (zh) 2024-01-01
AR113772A1 (es) 2020-06-10
JP2023156504A (ja) 2023-10-24
US11193103B2 (en) 2021-12-07
EA202090740A1 (ru) 2020-07-07
EP4039786A1 (en) 2022-08-10
ES2919850T3 (es) 2022-07-28
IL273150A (en) 2020-04-30
HUE058924T2 (hu) 2022-09-28
SG11202002339XA (en) 2020-04-29
EP3697887B1 (en) 2022-04-06
AU2018350898A1 (en) 2020-04-16
US20220056398A1 (en) 2022-02-24
CN111212899A (zh) 2020-05-29
JP7337784B2 (ja) 2023-09-04
WO2019079188A1 (en) 2019-04-25
JP2020536521A (ja) 2020-12-17

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