SG10201901708SA - Perfusion culturing methods and uses thereof - Google Patents

Perfusion culturing methods and uses thereof

Info

Publication number
SG10201901708SA
SG10201901708SA SG10201901708SA SG10201901708SA SG10201901708SA SG 10201901708S A SG10201901708S A SG 10201901708SA SG 10201901708S A SG10201901708S A SG 10201901708SA SG 10201901708S A SG10201901708S A SG 10201901708SA SG 10201901708S A SG10201901708S A SG 10201901708SA
Authority
SG
Singapore
Prior art keywords
degrees
culture medium
culturing
rpm
methods
Prior art date
Application number
SG10201901708SA
Inventor
Jianguo Yang
Agata Villiger-Oberbek
Yang Yang
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of SG10201901708SA publication Critical patent/SG10201901708SA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Abstract

PERFUSION CULTURING METHODS AND USES THEREOF A BSTRACT Provided herein are improved methods of culturing a mammalian cell that include providing conical containing mammalian suspended about % to 5 about 8 0% of the volume of the container of a first liquid culture medium; incuba ting the container for a period of time at about 31  C to about 40  C at a reactor angle of about 5 degrees to about 85 degrees (e.g., about 40 degrees to about 55 degrees) from horizontal and with an agita tion of about 2 0 revoluti ons per minute (RPM) to about 1000 RPM; and continuously or periodically , during the period of time, removing a first volume of the 10 first liquid culture medium that is substantially free of mammalian cells and adding to the first l iquid culture medium a second volume of a second liquid culture medium, wherein the first and second volumes are about equal, and methods of culturing a mammalian cell that include culturing in a gradient perfusion process a mammalian cell suspended in a l iquid culture medium under conditions that generate in the medium a fluid sheer force 15 and dissolved oxygen (O 2 ) concentration that is the same as (or essentially the same as ) that achieved in a gas -permeable coni cal container containing 4% to 4 0% volume of the container of liquid culture medium when p ositioned at a reactor angle of about 5 degrees to 85 degrees (e.g., 40 to 55 from , incubate d at a temperature of about 31  C to about 40  C, and agi tated at a freq uency of 20 about 2 0 revolutions per mi nute (RPM) to about 1000 RPM . Also provided are various methods that utilize these culturing methods. [Figure 1] 25
SG10201901708SA 2012-10-23 2013-10-23 Perfusion culturing methods and uses thereof SG10201901708SA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261717486P 2012-10-23 2012-10-23

Publications (1)

Publication Number Publication Date
SG10201901708SA true SG10201901708SA (en) 2019-03-28

Family

ID=49546634

Family Applications (2)

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SG11201503085VA SG11201503085VA (en) 2012-10-23 2013-10-23 Perfusion culturing methods and uses thereof
SG10201901708SA SG10201901708SA (en) 2012-10-23 2013-10-23 Perfusion culturing methods and uses thereof

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201503085VA SG11201503085VA (en) 2012-10-23 2013-10-23 Perfusion culturing methods and uses thereof

Country Status (9)

Country Link
US (1) US20140154726A1 (en)
EP (2) EP2912160B1 (en)
CN (2) CN108192822A (en)
AU (2) AU2013334602B2 (en)
CA (1) CA2889022A1 (en)
HK (1) HK1214292A1 (en)
MX (2) MX363702B (en)
SG (2) SG11201503085VA (en)
WO (1) WO2014066519A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201806083RA (en) * 2013-02-22 2018-08-30 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof
US10421949B2 (en) 2013-02-22 2019-09-24 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
AR095196A1 (en) * 2013-03-15 2015-09-30 Regeneron Pharma SERUM FREE CELL CULTIVATION MEDIA
TW202204596A (en) * 2014-06-06 2022-02-01 美商健臻公司 Perfusion culturing methods and uses thereof
TWI707949B (en) * 2014-06-09 2020-10-21 美商健臻公司 Seed train processes and uses thereof
RU2713126C2 (en) * 2014-10-24 2020-02-03 Джензим Корпорейшн Method for preventing contamination of system
IL308064A (en) 2014-12-22 2023-12-01 Genzyme Corp Methods of culturing a mammalian cell
CN105385731B (en) * 2015-12-25 2018-10-30 上海莱士血液制品股份有限公司 A kind of perfusion cultural method of eight factors of expression recombination
KR20180047404A (en) * 2016-10-31 2018-05-10 삼성바이오에피스 주식회사 Process for reducing undesirable by-product in cell culture
KR102604992B1 (en) * 2017-03-31 2023-11-24 베링거 인겔하임 인터내셔날 게엠베하 perfusion medium
US20200323958A1 (en) * 2017-11-13 2020-10-15 Amgen Inc. Methods for producing protein products

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2411178B8 (en) * 2004-02-20 2008-07-11 Cesco Bioengineering Co Ltd Apparatus and method for preparing and culturing cells
DK1891205T3 (en) * 2005-06-15 2018-03-19 Zhejiang Jinyishengshi Bioengineering Co Ltd SUSPENSION CULTURE CONTAINERS
WO2006138143A1 (en) * 2005-06-15 2006-12-28 Amprotein Corporation Suspension culture vessels
WO2008024769A2 (en) * 2006-08-21 2008-02-28 Raven Biotechnologies, Inc. Intensified perfusion production method
WO2009034186A2 (en) * 2007-09-13 2009-03-19 Helmholtz-Zentrum für Infektionsforschung GmbH Process for cell cultivation
US20090233334A1 (en) * 2008-03-11 2009-09-17 Excellgene Sa Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale
US20100076380A1 (en) * 2008-06-16 2010-03-25 Amprotein Corporation Bioreactors
WO2010120514A2 (en) 2009-03-31 2010-10-21 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds

Also Published As

Publication number Publication date
HK1214292A1 (en) 2016-07-22
AU2013334602B2 (en) 2018-05-31
MX363702B (en) 2019-03-29
MX2019003638A (en) 2019-09-02
US20140154726A1 (en) 2014-06-05
WO2014066519A1 (en) 2014-05-01
AU2013334602A1 (en) 2015-05-14
MX2015005206A (en) 2016-08-03
AU2018222985A1 (en) 2018-09-20
EP2912160A1 (en) 2015-09-02
CA2889022A1 (en) 2014-05-01
CN104903436A (en) 2015-09-09
SG11201503085VA (en) 2015-05-28
EP2912160B1 (en) 2020-07-29
EP3747984A1 (en) 2020-12-09
CN108192822A (en) 2018-06-22
CN104903436B (en) 2018-03-30

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