MX2022001892A - Metodos de cultivo de celula. - Google Patents

Metodos de cultivo de celula.

Info

Publication number
MX2022001892A
MX2022001892A MX2022001892A MX2022001892A MX2022001892A MX 2022001892 A MX2022001892 A MX 2022001892A MX 2022001892 A MX2022001892 A MX 2022001892A MX 2022001892 A MX2022001892 A MX 2022001892A MX 2022001892 A MX2022001892 A MX 2022001892A
Authority
MX
Mexico
Prior art keywords
cell culture
culture methods
acid
culture medium
metabolite
Prior art date
Application number
MX2022001892A
Other languages
English (en)
Inventor
Seongkyu Yoon
Bingyu Kuang
Shaun Galbraith
Original Assignee
Univ Massachusetts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Massachusetts filed Critical Univ Massachusetts
Publication of MX2022001892A publication Critical patent/MX2022001892A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7206—Mass spectrometers interfaced to gas chromatograph
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

Abstract

Un método de cultivo de célula incluye (i) cultivar células en un medio de cultivo de célula, y (ii) mantener al menos un metabolito seleccionado a partir de ácido aconítico (AA), ácido leucínico (HICA), monofosfato de citidina (CMP), ácido metilsuccínico (MSA), trigonelino (TRI) y N-acetilputrescinio (NAP), por debajo de una concentración inhibidora en el medio de cultivo de célula para el al menos un metabolito.
MX2022001892A 2019-08-14 2020-08-14 Metodos de cultivo de celula. MX2022001892A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962886683P 2019-08-14 2019-08-14
PCT/US2020/046330 WO2021030672A1 (en) 2019-08-14 2020-08-14 Cell culture methods

Publications (1)

Publication Number Publication Date
MX2022001892A true MX2022001892A (es) 2022-03-17

Family

ID=74570733

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022001892A MX2022001892A (es) 2019-08-14 2020-08-14 Metodos de cultivo de celula.

Country Status (11)

Country Link
US (2) US20220325235A1 (es)
EP (1) EP4013854A4 (es)
JP (1) JP2022546230A (es)
KR (1) KR20220083669A (es)
CN (1) CN114616321A (es)
AU (1) AU2020328052A1 (es)
BR (1) BR112022002765A2 (es)
CA (1) CA3150661A1 (es)
IL (1) IL290590A (es)
MX (1) MX2022001892A (es)
WO (2) WO2021030672A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2021002279A (es) 2018-08-27 2021-05-27 Regeneron Pharma Uso de espectroscopia raman en la purificacion corriente abajo.
BR112022002765A2 (pt) * 2019-08-14 2022-05-10 Univ Massachusetts Métodos para cultura de células
CN113466470B (zh) * 2021-07-26 2023-07-25 四川农业大学 响应面法优化萌芽种子蛋白最佳缓冲液萃取试验方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015140708A1 (en) * 2014-03-19 2015-09-24 Pfizer Inc. Method of cell culture
US20170145449A1 (en) * 2014-06-11 2017-05-25 Institut National De La Recherche Agronomique Improved lipid accumulation in Yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof
BR112022002765A2 (pt) * 2019-08-14 2022-05-10 Univ Massachusetts Métodos para cultura de células

Also Published As

Publication number Publication date
AU2020328052A1 (en) 2022-03-03
CN114616321A (zh) 2022-06-10
JP2022546230A (ja) 2022-11-04
CA3150661A1 (en) 2021-02-18
IL290590A (en) 2022-04-01
WO2023154910A3 (en) 2023-10-05
EP4013854A4 (en) 2023-10-11
WO2021030672A1 (en) 2021-02-18
KR20220083669A (ko) 2022-06-20
US20220325235A1 (en) 2022-10-13
EP4013854A1 (en) 2022-06-22
WO2023154910A2 (en) 2023-08-17
US20220204919A1 (en) 2022-06-30
WO2023154910A9 (en) 2023-12-07
BR112022002765A2 (pt) 2022-05-10

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