AR113772A1 - Biorreactor de perfusión y métodos de uso relacionados - Google Patents

Biorreactor de perfusión y métodos de uso relacionados

Info

Publication number
AR113772A1
AR113772A1 ARP180103002A ARP180103002A AR113772A1 AR 113772 A1 AR113772 A1 AR 113772A1 AR P180103002 A ARP180103002 A AR P180103002A AR P180103002 A ARP180103002 A AR P180103002A AR 113772 A1 AR113772 A1 AR 113772A1
Authority
AR
Argentina
Prior art keywords
bioreactor
cell culture
culture
cell
predetermined rate
Prior art date
Application number
ARP180103002A
Other languages
English (en)
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=64110133&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR113772(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of AR113772A1 publication Critical patent/AR113772A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un método para controlar un sistema de biorreactor incluye proporcionar un cultivo celular en un biorreactor, en donde las condiciones en el biorreactor permiten que el cultivo celular produzca una proteína de interés (POI), medir parámetros del proceso (PP) del cultivo dentro del biorreactor mediante RAMAN, en donde los parámetros del proceso son seleccionados del grupo que consiste en concentración de nutrientes, concentración de células viables y atributos de la proteína, medir un peso predeterminado del biorreactor con el cultivo celular, extraer el medio agotado libre de células del cultivo celular empleando un primer conducto de salida a una primera tasa predeterminada, extraer las células del cultivo celular empleando un segundo conducto de salida a una segunda tasa predeterminada e introducir uno de medio fresco o nutrientes, o ambos, en el cultivo celular empleando un conducto de entrada a una tercera tasa predeterminada.
ARP180103002A 2017-10-16 2018-10-16 Biorreactor de perfusión y métodos de uso relacionados AR113772A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762572918P 2017-10-16 2017-10-16

Publications (1)

Publication Number Publication Date
AR113772A1 true AR113772A1 (es) 2020-06-10

Family

ID=64110133

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180103002A AR113772A1 (es) 2017-10-16 2018-10-16 Biorreactor de perfusión y métodos de uso relacionados

Country Status (18)

Country Link
US (2) US11193103B2 (es)
EP (2) EP3697887B1 (es)
JP (2) JP7337784B2 (es)
KR (1) KR20200062236A (es)
CN (1) CN111212899A (es)
AR (1) AR113772A1 (es)
AU (1) AU2018350898A1 (es)
CA (1) CA3075679A1 (es)
DK (1) DK3697887T3 (es)
EA (1) EA202090740A1 (es)
ES (1) ES2919850T3 (es)
HU (1) HUE058924T2 (es)
IL (1) IL273150A (es)
MX (1) MX2020002851A (es)
PL (1) PL3697887T3 (es)
SG (1) SG11202002339XA (es)
TW (1) TWI827555B (es)
WO (1) WO2019079188A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11609120B2 (en) 2017-10-06 2023-03-21 Lonza Ltd Automated control of cell culture using Raman spectroscopy
CA3075679A1 (en) * 2017-10-16 2019-04-25 Regeneron Pharmaceuticals, Inc. Perfusion bioreactor and related methods of use
KR20210052389A (ko) 2018-08-27 2021-05-10 리제너론 파마슈티칼스 인코포레이티드 다운스트림 정제에서의 라만 분광법의 사용
US11774287B2 (en) 2019-05-28 2023-10-03 WuXi Biologics Ireland Limited Raman spectroscopy integrated perfusion cell culture system for monitoring and auto-controlling perfusion cell culture
US20220259547A1 (en) * 2019-06-13 2022-08-18 Amgeng Inc. Automated biomass-based perfusion control in the manufacturing of biologics
US20220380712A1 (en) * 2019-11-07 2022-12-01 Merck Patent Gmbh Methods and systems for performing perfusion cell culture
AU2020419442A1 (en) 2020-01-02 2022-06-09 F. Hoffmann-La Roche Ag Single-use cell culture container with one or more in-situ online sensors
WO2021140002A1 (en) * 2020-01-07 2021-07-15 Cytiva Sweden Ab Systems and method for controlling fluid flow in bioreactors
KR20220129006A (ko) * 2020-01-15 2022-09-22 우시 바이올로직스 아일랜드 리미티드 배양 세포로부터 생산된 생체 물질을 연속적으로 수확하기 위한 장치 및 방법
KR102656244B1 (ko) 2020-10-26 2024-04-09 성균관대학교산학협력단 바이오리액터
CA3203589A1 (en) * 2020-12-01 2022-06-09 The Brigham And Women's Hospital, Inc. Spectrometry systems, methods, and applications
KR102590857B1 (ko) * 2021-08-25 2023-10-19 재단법인 아산사회복지재단 세포 배양 장치
US20230323264A1 (en) * 2022-04-07 2023-10-12 Mirror Biologics, Inc. Artificial lymph node bioreactor
JP2024030410A (ja) 2022-08-24 2024-03-07 横河電機株式会社 細胞培養システム、及び細胞培養方法
NL2033094B1 (en) * 2022-09-21 2024-03-26 Simabs Nv Method for controlling a perfusion process in a bioreactor

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0211241A3 (de) 1985-07-06 1989-06-28 Forschungszentrum Jülich Gmbh Verfahren zur Exoenzymgewinnung durch Bakterienkultur
EP0567738A3 (en) * 1992-05-01 1995-09-06 American Cyanamid Co Controlling perfusion rates in continuous bioreactor culture of animal cells
JP2000501614A (ja) 1995-12-15 2000-02-15 システミックス，インコーポレイテッド 高形質導入効率を有するレトロウイルスベクター上清を得るための方法
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
ITPD20030142A1 (it) 2003-06-27 2004-12-28 Livia Barenghi Bioreattore, in particolare per spettroscopia nmr
AU2004260106C1 (en) 2003-07-17 2010-12-09 Global Cell Solutions, Llc. Automated cell culture system and process
CN1930281A (zh) 2004-03-05 2007-03-14 帝斯曼知识产权资产管理有限公司 用于通过连续灌注和交互切向流来培养细胞的方法
WO2005084696A1 (en) 2004-03-08 2005-09-15 Universita'degli Studi Di Milano Methods and agents for controlling intestinal inflammation and mucosal immunity using agents interacting with tir8/sigirr
EP1844138A2 (en) * 2004-12-29 2007-10-17 Biogen Idec MA Inc. Bioreactor process control system and method
ATE557038T1 (de) 2005-06-03 2012-05-15 Ares Trading Sa Herstellung von rekombinantem il-18 bindendem protein
US8003408B2 (en) * 2005-12-29 2011-08-23 Intel Corporation Modification of metal nanoparticles for improved analyte detection by surface enhanced Raman spectroscopy (SERS)
WO2007089551A2 (en) * 2006-01-27 2007-08-09 Prescient Medical, Inc. Raman spectroscopic test strip systems
US8119368B2 (en) 2006-07-14 2012-02-21 Dsm Ip Assets B.V. Process for the culturing of cells
US9109193B2 (en) 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
ES2657055T3 (es) 2007-08-09 2018-03-01 Wyeth Llc Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores
WO2010016943A2 (en) 2008-08-08 2010-02-11 Biogen Idec Ma Inc. Nutrient monitoring and feedback control for increased bioproduct production
CN102482633A (zh) 2009-07-06 2012-05-30 弗·哈夫曼-拉罗切有限公司 真核细胞的培养方法
US8765415B2 (en) 2009-11-17 2014-07-01 Medarex, L.L.C. Methods for enhanced protein production
CN103328092B (zh) 2011-01-13 2017-06-13 DPx控股有限公司 振荡流微反应器
LT2837680T (lt) 2011-07-01 2020-07-10 Amgen Inc. Žinduolių ląstelių kultūra
WO2013052318A1 (en) * 2011-09-25 2013-04-11 Theranos, Inc. Systems and methods for multi-analysis
ES2898637T3 (es) * 2012-01-06 2022-03-08 Bend Res Inc Métodos y aparatos de espectroscopia dieléctrica
AU2013329318A1 (en) 2012-10-10 2015-05-14 Bayer Healthcare Llc Methods and systems for optimizing perfusion cell culture system
RU2768003C2 (ru) * 2013-03-08 2022-03-22 Джензим Корпорейшн Интегрированное непрерывное производство терапевтических белковых лекарственных веществ
EP2972238B1 (en) * 2013-03-15 2023-04-26 Biogen MA Inc. Use of raman spectroscopy to monitor culture medium
US10214718B2 (en) * 2013-07-01 2019-02-26 University Of Massachusetts Distributed perfusion bioreactor system for continuous culture of biological cells
EP3047013B1 (en) * 2013-09-16 2021-08-18 Genzyme Corporation Methods and systems for processing a cell culture
SG11201601681QA (en) 2013-10-11 2016-04-28 Regeneron Pharma Metabolically optimized cell culture
WO2015095809A1 (en) 2013-12-20 2015-06-25 Biogen Idec Ma Inc. Use of perfusion seed cultures to improve biopharmaceutical fed-batch production capacity and product quality
EP3164481A4 (en) * 2014-07-02 2018-03-21 Biogen MA Inc. Cross-scale modeling of bioreactor cultures using raman spectroscopy
EP3303561A2 (en) * 2015-05-29 2018-04-11 Biogen MA Inc. Cell culture methods and systems
CN107922919A (zh) * 2015-05-29 2018-04-17 勃林格殷格翰国际公司 连续培养中的细胞控制的灌注
CN106834117B (zh) * 2015-10-27 2018-11-23 苏州迪欧益生物科技有限公司 用于解除动物细胞贴附的酶反应器及智能酶反应工作站
AU2016348588A1 (en) 2015-11-02 2018-04-26 Ares Trading S.A. Methods for modulating production profiles of recombinant proteins in perfusion mode
EP3408371A1 (en) * 2016-01-26 2018-12-05 Boehringer Ingelheim International Gmbh Linked perfusion to continuous-flow stirred-tank reactor cell culture system
CN205528832U (zh) * 2016-02-26 2016-08-31 南京农业大学 一种体外灌流系统
US11609120B2 (en) 2017-10-06 2023-03-21 Lonza Ltd Automated control of cell culture using Raman spectroscopy
CA3075679A1 (en) * 2017-10-16 2019-04-25 Regeneron Pharmaceuticals, Inc. Perfusion bioreactor and related methods of use

Also Published As

Publication number Publication date
EP4039786A1 (en) 2022-08-10
US11193103B2 (en) 2021-12-07
CA3075679A1 (en) 2019-04-25
TWI827555B (zh) 2024-01-01
SG11202002339XA (en) 2020-04-29
HUE058924T2 (hu) 2022-09-28
TW201934744A (zh) 2019-09-01
JP7337784B2 (ja) 2023-09-04
JP2020536521A (ja) 2020-12-17
WO2019079188A1 (en) 2019-04-25
US20220056398A1 (en) 2022-02-24
US20190153381A1 (en) 2019-05-23
EP3697887B1 (en) 2022-04-06
EA202090740A1 (ru) 2020-07-07
IL273150A (en) 2020-04-30
KR20200062236A (ko) 2020-06-03
US11965153B2 (en) 2024-04-23
PL3697887T3 (pl) 2022-08-01
CN111212899A (zh) 2020-05-29
BR112020005209A2 (pt) 2020-09-15
ES2919850T3 (es) 2022-07-28
EP3697887A1 (en) 2020-08-26
DK3697887T3 (da) 2022-05-02
AU2018350898A1 (en) 2020-04-16
JP2023156504A (ja) 2023-10-24
MX2020002851A (es) 2020-07-24

Similar Documents

Publication Publication Date Title
AR113772A1 (es) Biorreactor de perfusión y métodos de uso relacionados
GB2555728A (en) Cancer cell enrichment system
CL2020002805A1 (es) Método para identificar un modulador de un sistema biológico; método para identificar un modulador de un proceso de enfermedad (divisional 201302526)
WO2016172454A8 (en) Microfluidic cell culture
MX2018014681A (es) Biorreactor de un solo uso.
CL2008003629A1 (es) Metodo de analisis microbiologico de muestras biologicas que comprende un primer intervalo de tiempo de incubacion, tras el cual las muestras que dividen en 3 categorias (positivas, negativas o inciertas) dependiendo de la cantidad de dioxido de carbono generado, y donde las muestra que presentan un contenido de dioxido de carbono comprendido entre un valor maximo y minimo (muestras inciertas), se someten a un segundo intervalo de tiempo de incubacion y asi categorizarlas como positivas o negativas.
WO2016115537A3 (en) Systems, methods, and apparatus for in vitro single-cell identification and recovery
TR201900776T4 (tr) Memeli hücre kültürlerinin hasadına ilişkin yöntem.
EA201992795A1 (ru) Способ получения эукариотических клеток с отредактированной днк и набор, используемый в этом способе
MY171710A (en) Culture medium composition, and method for culturing cell or tissue using said composition
EA201590488A1 (ru) Способы модификации клеток-хозяев
CY1124672T1 (el) Μεθοδοι αποθηκευσης τραπεζων κυτταρων εξαιρετικα υψηλης πυκνοτητας
MX2020009648A (es) Muestreador de lecho fijo y metodos relacionados.
AR086200A1 (es) Hongos filamentosos que tienen un fenotipo de viscosidad alterado
WO2018053365A8 (en) METHODS OF PREPARING NUCLEIC ACID SAMPLE FOR ANALYSIS OF ACELLULAR DNA
BR112016028512A8 (pt) método de cultivo de uma célula de mamífero e método de produção de uma proteína recombinante
MX2021007433A (es) Metodos de cultivo de una celula de mamifero.
CO2019001319A2 (es) Metodo y sistema para farmacogenómica microbiana
SG10201901708SA (en) Perfusion culturing methods and uses thereof
CN103966318A (zh) 利用dna稳定性同位素探针原位揭示判别稻田甲酸利用型产甲烷古菌的方法
ES2652267T3 (es) Método para el aislamiento eficiente del ADN microbiano de la sangre
EP3190173A3 (en) Long-term storage medium for culturing obligate anaerobic bacteria or microaerobic bacteria under aerobic environment, and method of detecting obligate anaerobic bacteria or microaerobic bacteria using said medium
CO2018003988A2 (es) Un método de cultivo de células
PH12016501509A1 (en) Air accordion bioreactor
BR112016018797A2 (pt) Aperfeiçoamento de expressão de proteína recombinante com o uso de um sistema de retenção de célula baseado em membrana