AR113772A1 - Biorreactor de perfusión y métodos de uso relacionados - Google Patents

Biorreactor de perfusión y métodos de uso relacionados

Info

Publication number
AR113772A1
AR113772A1 ARP180103002A ARP180103002A AR113772A1 AR 113772 A1 AR113772 A1 AR 113772A1 AR P180103002 A ARP180103002 A AR P180103002A AR P180103002 A ARP180103002 A AR P180103002A AR 113772 A1 AR113772 A1 AR 113772A1
Authority
AR
Argentina
Prior art keywords
bioreactor
cell culture
culture
cell
predetermined rate
Prior art date
Application number
ARP180103002A
Other languages
English (en)
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=64110133&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR113772(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of AR113772A1 publication Critical patent/AR113772A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Un método para controlar un sistema de biorreactor incluye proporcionar un cultivo celular en un biorreactor, en donde las condiciones en el biorreactor permiten que el cultivo celular produzca una proteína de interés (POI), medir parámetros del proceso (PP) del cultivo dentro del biorreactor mediante RAMAN, en donde los parámetros del proceso son seleccionados del grupo que consiste en concentración de nutrientes, concentración de células viables y atributos de la proteína, medir un peso predeterminado del biorreactor con el cultivo celular, extraer el medio agotado libre de células del cultivo celular empleando un primer conducto de salida a una primera tasa predeterminada, extraer las células del cultivo celular empleando un segundo conducto de salida a una segunda tasa predeterminada e introducir uno de medio fresco o nutrientes, o ambos, en el cultivo celular empleando un conducto de entrada a una tercera tasa predeterminada.
ARP180103002A 2017-10-16 2018-10-16 Biorreactor de perfusión y métodos de uso relacionados AR113772A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762572918P 2017-10-16 2017-10-16

Publications (1)

Publication Number Publication Date
AR113772A1 true AR113772A1 (es) 2020-06-10

Family

ID=64110133

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180103002A AR113772A1 (es) 2017-10-16 2018-10-16 Biorreactor de perfusión y métodos de uso relacionados

Country Status (18)

Country Link
US (3) US11193103B2 (es)
EP (2) EP4039786A1 (es)
JP (2) JP7337784B2 (es)
KR (2) KR20240125704A (es)
CN (6) CN111212899B (es)
AR (1) AR113772A1 (es)
AU (1) AU2018350898B9 (es)
CA (1) CA3075679A1 (es)
DK (1) DK3697887T3 (es)
EA (1) EA202090740A1 (es)
ES (1) ES2919850T3 (es)
HU (1) HUE058924T2 (es)
IL (1) IL273150B1 (es)
MX (1) MX2020002851A (es)
PL (1) PL3697887T3 (es)
SG (1) SG11202002339XA (es)
TW (2) TW202426623A (es)
WO (1) WO2019079188A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3692358A1 (en) 2017-10-06 2020-08-12 Lonza Ltd Automated control of cell culture using raman spectroscopy
CN111212899B (zh) * 2017-10-16 2024-07-02 里珍纳龙药品有限公司 灌注生物反应器及相关使用方法
SG11202011970TA (en) 2018-08-27 2020-12-30 Regeneron Pharma Use of raman spectroscopy in downstream purification
CN113924355B (zh) * 2019-05-28 2024-04-02 上海药明生物技术有限公司 用于监测和自动控制灌流细胞培养的拉曼光谱集成灌流细胞培养系统
US20220259547A1 (en) * 2019-06-13 2022-08-18 Amgeng Inc. Automated biomass-based perfusion control in the manufacturing of biologics
JP2023500719A (ja) * 2019-11-07 2023-01-10 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング 灌流細胞培養を実施するための方法およびシステム
JP2023509434A (ja) 2020-01-02 2023-03-08 エフ． ホフマン－ラ ロシュ アーゲー １つ又は複数のｉｎ－ｓｉｔｕオンラインセンサーを備えた単回使用細胞培養容器
CN114867837A (zh) * 2020-01-07 2022-08-05 思拓凡瑞典有限公司 用于控制生物反应器中流体流的系统和方法
CN115003787A (zh) * 2020-01-15 2022-09-02 上海药明生物技术有限公司 连续收获由培养的细胞生产的生物物质的设备和方法
KR102656244B1 (ko) 2020-10-26 2024-04-09 성균관대학교산학협력단 바이오리액터
US20240090769A1 (en) * 2020-12-01 2024-03-21 The Brigham And Women's Hospital, Inc. Spectrometry systems, methods, and applications
KR102590857B1 (ko) * 2021-08-25 2023-10-19 재단법인 아산사회복지재단 세포 배양 장치
US20230323264A1 (en) * 2022-04-07 2023-10-12 Mirror Biologics, Inc. Artificial lymph node bioreactor
JP2024030410A (ja) 2022-08-24 2024-03-07 横河電機株式会社 細胞培養システム、及び細胞培養方法
NL2033094B1 (en) * 2022-09-21 2024-03-26 Simabs Nv Method for controlling a perfusion process in a bioreactor

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0211241A3 (de) 1985-07-06 1989-06-28 Forschungszentrum Jülich Gmbh Verfahren zur Exoenzymgewinnung durch Bakterienkultur
EP0567738A3 (en) * 1992-05-01 1995-09-06 American Cyanamid Co Controlling perfusion rates in continuous bioreactor culture of animal cells
CA2238434A1 (en) 1995-12-15 1997-06-19 Systemix, Inc. Method for obtaining retroviral vector supernatant having high transduction efficiency
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
ITPD20030142A1 (it) 2003-06-27 2004-12-28 Livia Barenghi Bioreattore, in particolare per spettroscopia nmr
CA2532754A1 (en) 2003-07-17 2005-02-03 Global Cell Solutions, Llc Automated cell culture system and process
EP2308958A3 (en) 2004-03-05 2011-08-10 DSM IP Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
WO2005084696A1 (en) 2004-03-08 2005-09-15 Universita'degli Studi Di Milano Methods and agents for controlling intestinal inflammation and mucosal immunity using agents interacting with tir8/sigirr
WO2006071716A2 (en) * 2004-12-29 2006-07-06 Biogen Idec Ma Inc. Bioreactor process control system and method
US7691611B2 (en) 2005-06-03 2010-04-06 Ares Trading S.A. Production of recombinant IL-18 binding protein
US8003408B2 (en) * 2005-12-29 2011-08-23 Intel Corporation Modification of metal nanoparticles for improved analyte detection by surface enhanced Raman spectroscopy (SERS)
WO2007089551A2 (en) * 2006-01-27 2007-08-09 Prescient Medical, Inc. Raman spectroscopic test strip systems
MX340242B (es) 2006-07-14 2016-07-01 Dpx Holdings Bv Proceso mejorado para el cultivo de celulas.
US9109193B2 (en) 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
EP2188371B1 (en) 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
EP2331700A2 (en) 2008-08-08 2011-06-15 Biogen Idec MA Inc. Nutrient monitoring and feedback control for increased bioproduct production
US20120329151A1 (en) 2009-07-06 2012-12-27 F. Hoffmann-La Roche Ag Method of culturing eukaryotic cells
HUE035508T2 (en) 2009-11-17 2018-05-02 Squibb & Sons Llc Procedures for Increased Protein Production
HUE057644T2 (hu) 2011-01-13 2022-05-28 Patheon Austria Gmbh & Co Kg Oszcilláló áramlású mini reaktor szilárd szuszpenziók feldolgozására
KR102558247B1 (ko) 2011-07-01 2023-07-24 암젠 인크 포유동물 세포 배양
RU2627927C2 (ru) * 2011-09-25 2017-08-14 Теранос, Инк. Системы и способы многостороннего анализа
WO2013103901A1 (en) * 2012-01-06 2013-07-11 Bend Research, Inc. Dielectric spectroscopy methods and apparatus
EP2906677A1 (en) 2012-10-10 2015-08-19 Bayer HealthCare LLC Methods and systems for optimizing perfusion cell culture system
WO2014137903A2 (en) * 2013-03-08 2014-09-12 Genzyme Corporation Integrated continuous manufacturing of therapeutic protein drug substances
US11249026B2 (en) * 2013-03-15 2022-02-15 Biogen Ma Inc. Use of raman spectroscopy to monitor culture medium
WO2015003012A2 (en) * 2013-07-01 2015-01-08 Olivier Berteau Distributed perfusion bioreactor system for continuous culture of biological cells
SG11201601899TA (en) * 2013-09-16 2016-04-28 Genzyme Corp Methods and systems for processing a cell culture
EA201991976A1 (ru) 2013-10-11 2020-04-02 Регенерон Фармасьютикалз, Инк. Метаболически оптимизированная клеточная культура
US20160289633A1 (en) 2013-12-20 2016-10-06 Biogen Ma Inc. Use of Perfusion Seed Cultures to Improve Biopharmaceutical Fed-Batch Production Capacity and Product Quality
WO2016004322A2 (en) * 2014-07-02 2016-01-07 Biogen Ma Inc. Cross-scale modeling of bioreactor cultures using raman spectroscopy
US20180291329A1 (en) 2015-05-29 2018-10-11 Biogen Ma Inc. Cell culture methods and systems
JP7340324B2 (ja) * 2015-05-29 2023-09-07 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 連続培養における細胞コントロール灌流
CN106834117B (zh) * 2015-10-27 2018-11-23 苏州迪欧益生物科技有限公司 用于解除动物细胞贴附的酶反应器及智能酶反应工作站
AU2016348588A1 (en) 2015-11-02 2018-04-26 Ares Trading S.A. Methods for modulating production profiles of recombinant proteins in perfusion mode
US11104875B2 (en) * 2016-01-26 2021-08-31 Boehringer Ingelheim International Gmbh Linked perfusion to continuous-flow stirred-tank reactor cell culture system
CN205528832U (zh) * 2016-02-26 2016-08-31 南京农业大学 一种体外灌流系统
EP3692358A1 (en) 2017-10-06 2020-08-12 Lonza Ltd Automated control of cell culture using raman spectroscopy
CN111212899B (zh) * 2017-10-16 2024-07-02 里珍纳龙药品有限公司 灌注生物反应器及相关使用方法

Also Published As

Publication number Publication date
EP3697887A1 (en) 2020-08-26
CN118580925A (zh) 2024-09-03
CN111212899A (zh) 2020-05-29
US11965153B2 (en) 2024-04-23
TW201934744A (zh) 2019-09-01
MX2020002851A (es) 2020-07-24
AU2018350898B2 (en) 2024-07-04
PL3697887T3 (pl) 2022-08-01
CN118580926A (zh) 2024-09-03
KR102694992B1 (ko) 2024-08-14
JP2020536521A (ja) 2020-12-17
EA202090740A1 (ru) 2020-07-07
US11193103B2 (en) 2021-12-07
TW202426623A (zh) 2024-07-01
HUE058924T2 (hu) 2022-09-28
BR112020005209A2 (pt) 2020-09-15
DK3697887T3 (da) 2022-05-02
KR20200062236A (ko) 2020-06-03
WO2019079188A1 (en) 2019-04-25
ES2919850T3 (es) 2022-07-28
KR20240125704A (ko) 2024-08-19
IL273150A (en) 2020-04-30
US20220056398A1 (en) 2022-02-24
IL273150B1 (en) 2024-10-01
JP7337784B2 (ja) 2023-09-04
SG11202002339XA (en) 2020-04-29
CN118580924A (zh) 2024-09-03
US20190153381A1 (en) 2019-05-23
EP4039786A1 (en) 2022-08-10
CN118580927A (zh) 2024-09-03
EP3697887B1 (en) 2022-04-06
AU2018350898B9 (en) 2024-07-25
AU2018350898A1 (en) 2020-04-16
US20240218313A1 (en) 2024-07-04
CN111212899B (zh) 2024-07-02
JP2023156504A (ja) 2023-10-24
CA3075679A1 (en) 2019-04-25
CN118580928A (zh) 2024-09-03
TWI827555B (zh) 2024-01-01

Similar Documents

Publication Publication Date Title
AR113772A1 (es) Biorreactor de perfusión y métodos de uso relacionados
CL2020002805A1 (es) Método para identificar un modulador de un sistema biológico; método para identificar un modulador de un proceso de enfermedad (divisional 201302526)
WO2016172454A8 (en) Microfluidic cell culture
MX2018014681A (es) Biorreactor de un solo uso.
CL2008003629A1 (es) Metodo de analisis microbiologico de muestras biologicas que comprende un primer intervalo de tiempo de incubacion, tras el cual las muestras que dividen en 3 categorias (positivas, negativas o inciertas) dependiendo de la cantidad de dioxido de carbono generado, y donde las muestra que presentan un contenido de dioxido de carbono comprendido entre un valor maximo y minimo (muestras inciertas), se someten a un segundo intervalo de tiempo de incubacion y asi categorizarlas como positivas o negativas.
BR112016028512A8 (pt) método de cultivo de uma célula de mamífero e método de produção de uma proteína recombinante
WO2016115537A3 (en) Systems, methods, and apparatus for in vitro single-cell identification and recovery
EA201992795A1 (ru) Способ получения эукариотических клеток с отредактированной днк и набор, используемый в этом способе
TR201900776T4 (tr) Memeli hücre kültürlerinin hasadına ilişkin yöntem.
CY1124672T1 (el) Μεθοδοι αποθηκευσης τραπεζων κυτταρων εξαιρετικα υψηλης πυκνοτητας
MY171710A (en) Culture medium composition, and method for culturing cell or tissue using said composition
CO2019001319A2 (es) Metodo y sistema para farmacogenómica microbiana
AR086200A1 (es) Hongos filamentosos que tienen un fenotipo de viscosidad alterado
EP4353830A3 (en) Methods of nucleic acid sample preparation for analysis of cell-free dna
MX2021007433A (es) Metodos de cultivo de una celula de mamifero.
SG10201901708SA (en) Perfusion culturing methods and uses thereof
CN103966318A (zh) 利用dna稳定性同位素探针原位揭示判别稻田甲酸利用型产甲烷古菌的方法
EP3190173A3 (en) Long-term storage medium for culturing obligate anaerobic bacteria or microaerobic bacteria under aerobic environment, and method of detecting obligate anaerobic bacteria or microaerobic bacteria using said medium
ES2652267T3 (es) Método para el aislamiento eficiente del ADN microbiano de la sangre
EP3730597A4 (en) CELL CULTURE APPARATUS, CULTURE SOLUTION ASPIRATOR AND CELL CULTURE PROCESS
ES2660763T3 (es) Sonda codificada genéticamente para la cuantificación de la concentración de piruvato y métodos de utilización del mismo
BR112016018797A2 (pt) Aperfeiçoamento de expressão de proteína recombinante com o uso de um sistema de retenção de célula baseado em membrana
BR112012033050A2 (pt) método e sistema para cultivo de microalgas em um reator de fluxo de tampão expandido
PL403996A1 (pl) Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metodą PCR, startery oraz zestaw do detekcji bakterii i grzybów
MY197963A (en) Algae treatment method