WO2007115337A8 - Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering - Google Patents

Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering

Info

Publication number
WO2007115337A8
WO2007115337A8 PCT/US2007/066092 US2007066092W WO2007115337A8 WO 2007115337 A8 WO2007115337 A8 WO 2007115337A8 US 2007066092 W US2007066092 W US 2007066092W WO 2007115337 A8 WO2007115337 A8 WO 2007115337A8
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
embryonic stem
human embryonic
tissue engineering
chondrocyte differentiation
Prior art date
Application number
PCT/US2007/066092
Other languages
French (fr)
Other versions
WO2007115337A2 (en
WO2007115337A3 (en
Inventor
Kyriacos A Athanasiou
Gwendolyn Hoben
Eugene J Koay
Jerry Hu
Original Assignee
Univ Rice William M
Kyriacos A Athanasiou
Gwendolyn Hoben
Eugene J Koay
Jerry Hu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rice William M, Kyriacos A Athanasiou, Gwendolyn Hoben, Eugene J Koay, Jerry Hu filed Critical Univ Rice William M
Priority to AU2007234366A priority Critical patent/AU2007234366A1/en
Priority to CA002648327A priority patent/CA2648327A1/en
Priority to EP07760211A priority patent/EP2007881A4/en
Publication of WO2007115337A2 publication Critical patent/WO2007115337A2/en
Publication of WO2007115337A8 publication Critical patent/WO2007115337A8/en
Publication of WO2007115337A3 publication Critical patent/WO2007115337A3/en
Priority to US12/246,367 priority patent/US20090142307A1/en
Priority to US12/246,320 priority patent/US8637065B2/en
Priority to US12/246,306 priority patent/US20090136559A1/en
Priority to US13/029,325 priority patent/US20110212894A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/76—Agarose, agar-agar

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Rheumatology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Vascular Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Urology & Nephrology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Prostheses (AREA)
  • Materials For Medical Uses (AREA)

Abstract

Provided are improved methods for tissue engineering. More particularly, methods for inducing differentiation of human embryonic stem cells into chondrocytes for use in tissue engineering applications are provided. One example of a method is a method for inducing differentiation of human embryonic stem cells into chondrocytes comprising aggregating undifferentiated human embryonic stem cells to form embryoid bodies; and culturing the embryoid bodies in culture medium in the presence of growth factors that induce chondrogenic differentiation of the embryoid bodies.
PCT/US2007/066092 2004-07-09 2007-04-05 Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering WO2007115337A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2007234366A AU2007234366A1 (en) 2006-04-05 2007-04-05 Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering
CA002648327A CA2648327A1 (en) 2006-04-05 2007-04-05 Tissue engineering with human embryonic stem cells
EP07760211A EP2007881A4 (en) 2006-04-05 2007-04-05 Tissue engineering with human embryonic stem cells
US12/246,367 US20090142307A1 (en) 2004-07-09 2008-10-06 Shape-Based Approach for Scaffoldless Tissue Engineering
US12/246,320 US8637065B2 (en) 2004-07-09 2008-10-06 Dermis-derived cells for tissue engineering applications
US12/246,306 US20090136559A1 (en) 2004-07-09 2008-10-06 Chondrocyte Differentiation from Human Embryonic Stem Cells and Their Use in Tissue Engineering
US13/029,325 US20110212894A1 (en) 2004-07-09 2011-02-17 Decellularization method for scaffoldless tissue engineered articular cartilage or native cartilage tissue

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US78985106P 2006-04-05 2006-04-05
US78985506P 2006-04-05 2006-04-05
US78985306P 2006-04-05 2006-04-05
US60/789,855 2006-04-05
US60/789,853 2006-04-05
US60/789,851 2006-04-05

Related Child Applications (4)

Application Number Title Priority Date Filing Date
PCT/US2007/066085 Continuation-In-Part WO2007136936A2 (en) 2004-07-09 2007-04-05 Dermis-derived cells for tissue engineering applications
US12/246,367 Continuation-In-Part US20090142307A1 (en) 2004-07-09 2008-10-06 Shape-Based Approach for Scaffoldless Tissue Engineering
US12/246,306 Continuation-In-Part US20090136559A1 (en) 2004-07-09 2008-10-06 Chondrocyte Differentiation from Human Embryonic Stem Cells and Their Use in Tissue Engineering
US12/246,320 Continuation-In-Part US8637065B2 (en) 2004-07-09 2008-10-06 Dermis-derived cells for tissue engineering applications

Publications (3)

Publication Number Publication Date
WO2007115337A2 WO2007115337A2 (en) 2007-10-11
WO2007115337A8 true WO2007115337A8 (en) 2007-12-13
WO2007115337A3 WO2007115337A3 (en) 2008-07-03

Family

ID=38564326

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/US2007/066085 WO2007136936A2 (en) 2004-07-09 2007-04-05 Dermis-derived cells for tissue engineering applications
PCT/US2007/066089 WO2007115336A2 (en) 2004-07-09 2007-04-05 A shape-based approach for scaffoldless tissue engineering
PCT/US2007/066092 WO2007115337A2 (en) 2004-07-09 2007-04-05 Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering

Family Applications Before (2)

Application Number Title Priority Date Filing Date
PCT/US2007/066085 WO2007136936A2 (en) 2004-07-09 2007-04-05 Dermis-derived cells for tissue engineering applications
PCT/US2007/066089 WO2007115336A2 (en) 2004-07-09 2007-04-05 A shape-based approach for scaffoldless tissue engineering

Country Status (4)

Country Link
EP (3) EP2007881A4 (en)
AU (3) AU2007254048A1 (en)
CA (3) CA2648648A1 (en)
WO (3) WO2007136936A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058207A1 (en) 2003-12-11 2005-06-30 Isto Technologies, Inc. Particulate cartilage system
WO2005081970A2 (en) 2004-02-24 2005-09-09 The Curators Of The University Of Missouri Self-assembling cell aggregates and methods of making engineered tissue using the same
US20110212894A1 (en) * 2004-07-09 2011-09-01 Athanasiou Kyriacos A Decellularization method for scaffoldless tissue engineered articular cartilage or native cartilage tissue
WO2010022074A1 (en) * 2008-08-18 2010-02-25 William Marsh Rice University A decellularization method for scaffoldless tissue engineered articular cartilage or native cartilage tissue
WO2009111390A1 (en) * 2008-03-03 2009-09-11 William Marsh Rice University Methods of fabricating enhanced tissue-engineered cartilage
WO2007025290A2 (en) 2005-08-26 2007-03-01 Isto Technologies, Inc. Implants and methods for repair, replacement and treatment of joint disease
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
US20090012629A1 (en) 2007-04-12 2009-01-08 Isto Technologies, Inc. Compositions and methods for tissue repair
US9303245B2 (en) * 2008-06-20 2016-04-05 Universiteit Maastricht Self-assembling tissue modules
KR20170020942A (en) 2008-06-24 2017-02-24 더 큐레이터스 오브 더 유니버시티 오브 미주리 Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same
CA2768376C (en) * 2009-07-16 2023-01-03 Biotime, Inc. Methods and compositions for in vitro and in vivo chondrogenesis
DK2629975T3 (en) 2010-10-21 2022-05-09 Organovo Inc TISSUE MANUFACTURING DEVICES
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
US9499779B2 (en) 2012-04-20 2016-11-22 Organovo, Inc. Devices, systems, and methods for the fabrication of tissue utilizing UV cross-linking
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
US9442105B2 (en) 2013-03-15 2016-09-13 Organovo, Inc. Engineered liver tissues, arrays thereof, and methods of making the same
CA2919734C (en) 2013-07-31 2023-04-25 Organovo, Inc. Automated devices, systems, and methods for the fabrication of tissue
CA3177480A1 (en) 2014-04-04 2015-10-08 Organovo, Inc. Engineered three-dimensional breast tissue, adipose tissue, and tumor disease model
US9670443B2 (en) * 2014-06-25 2017-06-06 University Of Leeds Tissue engineered constructs
KR20170064547A (en) 2014-10-06 2017-06-09 오가노보, 인크. Engineered renal tissues, arrays thereof, and methods of making the same
WO2016073782A1 (en) 2014-11-05 2016-05-12 Organovo, Inc. Engineered three-dimensional skin tissues, arrays thereof, and methods of making the same

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE258810T1 (en) * 1995-11-09 2004-02-15 Univ Massachusetts RESTORATION OF TISSUE SURFACE USING HYDROGEL CELL COMPOSITIONS
EP1159404A2 (en) * 1999-03-02 2001-12-05 University of Massachusetts, a Public Institution of Higher Education of The Commonwealth of Massachusetts, Embryonic or stem-like cell lines produced by cross species nuclear transplantation
IL154159A0 (en) * 2000-08-01 2003-07-31 Yissum Res Dev Co Directed differentiation of ebryonic cells
AU2002258477A1 (en) * 2001-03-08 2002-09-24 Advanced Cell Technology, Inc. Use of rna interference for the creation of lineage specific es and other undifferentiated cells and production of differentiated cells in vitro by co-culture
KR100973453B1 (en) * 2001-12-07 2010-08-02 제론 코포레이션 Chondrocyte precursors derived from human embryonic stem cells
US20020106625A1 (en) * 2002-02-07 2002-08-08 Hung Clark T. Bioreactor for generating functional cartilaginous tissue
EP1490477A4 (en) * 2002-04-02 2006-08-30 Univ Rice William M Redifferentiated cells for repairing cartilage defects
US20040082063A1 (en) * 2002-10-18 2004-04-29 Reliance Life Sciences Pvt. Ltd. Tissue-like organization of cells and macroscopic tissue-like constructs, generated by macromass culture of cells, and the method of macromass culture
WO2006017176A2 (en) * 2004-07-09 2006-02-16 William Marsh Rice University Scaffoldless constructs for tissue engineering of articular cartilage

Also Published As

Publication number Publication date
EP2007312A2 (en) 2008-12-31
AU2007254048A1 (en) 2007-11-29
WO2007136936A2 (en) 2007-11-29
CA2648648A1 (en) 2007-11-29
AU2007234365A1 (en) 2007-10-11
EP2007881A2 (en) 2008-12-31
WO2007115337A2 (en) 2007-10-11
WO2007115336A3 (en) 2008-05-22
EP2007875A2 (en) 2008-12-31
EP2007875A4 (en) 2009-12-16
CA2648332A1 (en) 2007-10-11
EP2007312A4 (en) 2012-08-22
WO2007136936A3 (en) 2008-10-23
AU2007234366A1 (en) 2007-10-11
WO2007115336A2 (en) 2007-10-11
EP2007881A4 (en) 2009-07-08
CA2648327A1 (en) 2007-10-11
WO2007115337A3 (en) 2008-07-03

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