WO2012052854A3 - Plant cell lines and methods of isolating the same - Google Patents
Plant cell lines and methods of isolating the same Download PDFInfo
- Publication number
- WO2012052854A3 WO2012052854A3 PCT/IB2011/003287 IB2011003287W WO2012052854A3 WO 2012052854 A3 WO2012052854 A3 WO 2012052854A3 IB 2011003287 W IB2011003287 W IB 2011003287W WO 2012052854 A3 WO2012052854 A3 WO 2012052854A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plant
- cell
- biologically active
- isolating
- cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56961—Plant cells or fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Environmental Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
An isolated plant cell having increased expression of one or more of a PXY and WOL nucleic acid molecule or polypeptide, a method of isolating a plant stem cell, a method of producing a plant-derived biologically active substance using the isolated plant stem cell are disclosed. The cells are undifferentiated, undergo self-renewal, proliferate, and have the ability to differentiate. Cultures of the plant cells minimize variation of cell growth and stably produce biologically active substances, even in long-term culture. Production of biologically active substances using the plant cells solves problems of dedifferentiated plant cell cultures, including decreased cell growth and decreased productivity during long term culture.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US40612910P | 2010-10-23 | 2010-10-23 | |
US61/406,129 | 2010-10-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2012052854A2 WO2012052854A2 (en) | 2012-04-26 |
WO2012052854A3 true WO2012052854A3 (en) | 2012-08-02 |
Family
ID=45975670
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2011/003287 WO2012052854A2 (en) | 2010-10-23 | 2011-10-24 | Plant cell lines and methods of isolating the same |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2012052854A2 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2902808C (en) * | 2013-02-28 | 2018-10-09 | Unhwa Corporation | Recombinant plant cells, production method thereof and method of producing target protein using the same |
US9681615B2 (en) | 2013-06-20 | 2017-06-20 | Elwha Llc | Rapid breeding of plants |
US20140380519A1 (en) * | 2013-06-20 | 2014-12-25 | Elwha Llc | Rapid breeding of plants |
CN103805594B (en) * | 2014-02-17 | 2016-06-29 | 湖南农业大学 | A kind of cell hybridization produces the method for arteannuin |
CN104195098A (en) * | 2014-09-22 | 2014-12-10 | 古焕庆 | Dendrobium officinale stem cell and isolated culture method thereof |
CN108967200B (en) * | 2018-09-30 | 2020-11-13 | 广州赛莱拉干细胞科技股份有限公司 | Induction medium for plant callus and preparation method for plant stem cells |
CN109321611A (en) * | 2018-10-22 | 2019-02-12 | 覃家日 | The production method of taxol |
CN109576209B (en) * | 2019-01-24 | 2021-08-03 | 深圳先声科技发展有限公司 | Ginseng cambium stem cell separation culture method |
CN112616672A (en) * | 2020-12-31 | 2021-04-09 | 云南农业大学 | Method for directly inducing seedling emergence by utilizing stem segments of panax notoginseng |
GB2620405A (en) * | 2022-07-06 | 2024-01-10 | Green Bioactives Ltd | Method for isolating plant stem cells from plant leaves and the associated cell lines obtained utilising the method |
CN115948494B (en) * | 2022-09-12 | 2024-07-16 | 昆明理工大学 | Method for synthesizing oleanane-type saponin by using pseudo-ginseng cells |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007052876A1 (en) * | 2005-10-31 | 2007-05-10 | Unhwa | Stability of secondary metabolite mass production through syncronized plant cell cultures |
WO2009048306A1 (en) * | 2007-10-10 | 2009-04-16 | Unhwa Biotech. Corp. | Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium |
WO2010053314A2 (en) * | 2008-11-06 | 2010-05-14 | 주식회사 운화바이오텍 | Composition for cancer prevention or treatment containing as active ingredient plant stem cell line derived from cambium of panax ginseng including wild ginseng or ginseng |
-
2011
- 2011-10-24 WO PCT/IB2011/003287 patent/WO2012052854A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007052876A1 (en) * | 2005-10-31 | 2007-05-10 | Unhwa | Stability of secondary metabolite mass production through syncronized plant cell cultures |
WO2009048306A1 (en) * | 2007-10-10 | 2009-04-16 | Unhwa Biotech. Corp. | Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium |
WO2010053314A2 (en) * | 2008-11-06 | 2010-05-14 | 주식회사 운화바이오텍 | Composition for cancer prevention or treatment containing as active ingredient plant stem cell line derived from cambium of panax ginseng including wild ginseng or ginseng |
Non-Patent Citations (1)
Title |
---|
LEE ET AL.: "Cultured cambial meristematic cells as a source of plant natural products", NATURE BIOTECHNOLOGY, vol. 28, no. 11, 24 October 2010 (2010-10-24), pages 1213 - 1217 * |
Also Published As
Publication number | Publication date |
---|---|
WO2012052854A2 (en) | 2012-04-26 |
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