WO2012052854A3 - Plant cell lines and methods of isolating the same - Google Patents

Plant cell lines and methods of isolating the same Download PDF

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Publication number
WO2012052854A3
WO2012052854A3 PCT/IB2011/003287 IB2011003287W WO2012052854A3 WO 2012052854 A3 WO2012052854 A3 WO 2012052854A3 IB 2011003287 W IB2011003287 W IB 2011003287W WO 2012052854 A3 WO2012052854 A3 WO 2012052854A3
Authority
WO
WIPO (PCT)
Prior art keywords
plant
cell
biologically active
isolating
cells
Prior art date
Application number
PCT/IB2011/003287
Other languages
French (fr)
Other versions
WO2012052854A2 (en
Inventor
Eun Kyong Lee
Young Woo Jin
Joong Hyun Park
Il Seok Oh
Min Jung Lim
Gary John Loake
Byung-Wook Yun
Thomas Waibel
Original Assignee
Unhwa Corporation
University Of Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unhwa Corporation, University Of Edinburgh filed Critical Unhwa Corporation
Publication of WO2012052854A2 publication Critical patent/WO2012052854A2/en
Publication of WO2012052854A3 publication Critical patent/WO2012052854A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

An isolated plant cell having increased expression of one or more of a PXY and WOL nucleic acid molecule or polypeptide, a method of isolating a plant stem cell, a method of producing a plant-derived biologically active substance using the isolated plant stem cell are disclosed. The cells are undifferentiated, undergo self-renewal, proliferate, and have the ability to differentiate. Cultures of the plant cells minimize variation of cell growth and stably produce biologically active substances, even in long-term culture. Production of biologically active substances using the plant cells solves problems of dedifferentiated plant cell cultures, including decreased cell growth and decreased productivity during long term culture.
PCT/IB2011/003287 2010-10-23 2011-10-24 Plant cell lines and methods of isolating the same WO2012052854A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40612910P 2010-10-23 2010-10-23
US61/406,129 2010-10-23

Publications (2)

Publication Number Publication Date
WO2012052854A2 WO2012052854A2 (en) 2012-04-26
WO2012052854A3 true WO2012052854A3 (en) 2012-08-02

Family

ID=45975670

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2011/003287 WO2012052854A2 (en) 2010-10-23 2011-10-24 Plant cell lines and methods of isolating the same

Country Status (1)

Country Link
WO (1) WO2012052854A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014133365A1 (en) * 2013-02-28 2014-09-04 주식회사 운화 Recombinant plant cell, preparation method therefor, and method for producing target protein using same
US20140380519A1 (en) * 2013-06-20 2014-12-25 Elwha Llc Rapid breeding of plants
US9681615B2 (en) 2013-06-20 2017-06-20 Elwha Llc Rapid breeding of plants
CN103805594B (en) * 2014-02-17 2016-06-29 湖南农业大学 A kind of cell hybridization produces the method for arteannuin
CN104195098A (en) * 2014-09-22 2014-12-10 古焕庆 Dendrobium officinale stem cell and isolated culture method thereof
CN108967200B (en) * 2018-09-30 2020-11-13 广州赛莱拉干细胞科技股份有限公司 Induction medium for plant callus and preparation method for plant stem cells
CN109321611A (en) * 2018-10-22 2019-02-12 覃家日 The production method of taxol
CN109576209B (en) * 2019-01-24 2021-08-03 深圳先声科技发展有限公司 Ginseng cambium stem cell separation culture method
CN112616672A (en) * 2020-12-31 2021-04-09 云南农业大学 Method for directly inducing seedling emergence by utilizing stem segments of panax notoginseng
GB2620405A (en) * 2022-07-06 2024-01-10 Green Bioactives Ltd Method for isolating plant stem cells from plant leaves and the associated cell lines obtained utilising the method
CN115948494A (en) * 2022-09-12 2023-04-11 昆明理工大学 Method for synthesizing oleanane type saponin by using panax notoginseng cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007052876A1 (en) * 2005-10-31 2007-05-10 Unhwa Stability of secondary metabolite mass production through syncronized plant cell cultures
WO2009048306A1 (en) * 2007-10-10 2009-04-16 Unhwa Biotech. Corp. Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium
WO2010053314A2 (en) * 2008-11-06 2010-05-14 주식회사 운화바이오텍 Composition for cancer prevention or treatment containing as active ingredient plant stem cell line derived from cambium of panax ginseng including wild ginseng or ginseng

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007052876A1 (en) * 2005-10-31 2007-05-10 Unhwa Stability of secondary metabolite mass production through syncronized plant cell cultures
WO2009048306A1 (en) * 2007-10-10 2009-04-16 Unhwa Biotech. Corp. Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium
WO2010053314A2 (en) * 2008-11-06 2010-05-14 주식회사 운화바이오텍 Composition for cancer prevention or treatment containing as active ingredient plant stem cell line derived from cambium of panax ginseng including wild ginseng or ginseng

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEE ET AL.: "Cultured cambial meristematic cells as a source of plant natural products", NATURE BIOTECHNOLOGY, vol. 28, no. 11, 24 October 2010 (2010-10-24), pages 1213 - 1217 *

Also Published As

Publication number Publication date
WO2012052854A2 (en) 2012-04-26

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