DK496385A - Reguleringsregion for heterolog genekspression i gaer - Google Patents

Reguleringsregion for heterolog genekspression i gaer Download PDF

Info

Publication number
DK496385A
DK496385A DK496385A DK496385A DK496385A DK 496385 A DK496385 A DK 496385A DK 496385 A DK496385 A DK 496385A DK 496385 A DK496385 A DK 496385A DK 496385 A DK496385 A DK 496385A
Authority
DK
Denmark
Prior art keywords
yeast
gene expression
heterologous gene
regulation region
regulation
Prior art date
Application number
DK496385A
Other languages
Danish (da)
English (en)
Other versions
DK496385D0 (da
Inventor
David Womack Stroman
Paul Frederick Brust
Steven Bradley Ellis
Thomas Raymond Gingeras
Michael Miller Harpold
Juerg Friedrich Tschopp
Original Assignee
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/666,391 external-priority patent/US4808537A/en
Application filed by Phillips Petroleum Co filed Critical Phillips Petroleum Co
Publication of DK496385D0 publication Critical patent/DK496385D0/da
Publication of DK496385A publication Critical patent/DK496385A/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
DK496385A 1984-10-30 1985-10-29 Reguleringsregion for heterolog genekspression i gaer DK496385A (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use
US06/780,102 US4855231A (en) 1984-10-30 1985-09-25 Regulatory region for heterologous gene expression in yeast

Publications (2)

Publication Number Publication Date
DK496385D0 DK496385D0 (da) 1985-10-29
DK496385A true DK496385A (da) 1986-05-01

Family

ID=27099454

Family Applications (1)

Application Number Title Priority Date Filing Date
DK496385A DK496385A (da) 1984-10-30 1985-10-29 Reguleringsregion for heterolog genekspression i gaer

Country Status (23)

Country Link
US (1) US4855231A (cs)
EP (2) EP0183071B1 (cs)
JP (5) JP2502508B2 (cs)
KR (1) KR930001117B1 (cs)
CN (1) CN1011243B (cs)
AR (1) AR242988A1 (cs)
AT (1) ATE83263T1 (cs)
AU (1) AU572002B2 (cs)
CA (1) CA1340733C (cs)
DD (1) DD254211A5 (cs)
DE (1) DE3586889T2 (cs)
DK (1) DK496385A (cs)
ES (1) ES8609458A1 (cs)
FI (1) FI94427C (cs)
GR (1) GR852611B (cs)
HU (1) HU205627B (cs)
IE (1) IE930804L (cs)
IL (1) IL76765A (cs)
NO (1) NO178076C (cs)
NZ (1) NZ213842A (cs)
PL (1) PL158965B1 (cs)
PT (1) PT81399B (cs)
YU (1) YU46695B (cs)

Families Citing this family (117)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
IL81817A0 (en) * 1986-03-14 1987-10-20 Phillips Petroleum Co Methanol and glucose responsive yeast regulatory regions
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
DE3789866T2 (de) * 1987-07-17 1994-09-22 Rhein Biotech Gesellschaft Fuer Neue Biotechnologische Prozesse Und Produkte Mbh, 40595 Duesseldorf DNA-Moleküle, die für FMDH-Kontrollabschnitte und Strukturgene für ein Protein mit FMDH-Aktivität kodieren, sowie deren Anwendung.
EP0327797B1 (de) * 1988-01-05 2003-09-03 Roche Diagnostics GmbH Verfahren zur Herstellung von Proteinen oder proteinhaltigen Genprodukten
FI891695A7 (fi) * 1988-04-11 1989-10-12 Phillips Petroleum Co Lohen kasvuhormoonin ilmentäminen sukuun Pichia kuuluvassa metylotroofisessa hiivassa
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
IL89991A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of hepatitis b pres2 protein in methylotrophic yeasts
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
DE3851535T2 (de) * 1988-12-20 1995-02-02 Rhein Biotech Proz & Prod Gmbh Mutanter Stamm von einem methylotrophen Organismus und Verfahren zur Herstellung eines Proteins in methylotrophen Organismus.
AU5196290A (en) * 1989-02-13 1990-09-05 Salk Institute Biotechnology/Industrial Associates, Inc., The Production of superoxide dismutase in pichia pastoris yeast cells
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US5369028A (en) * 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
US6440681B1 (en) 1990-04-03 2002-08-27 Merck & Co., Inc. Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
WO1992017595A1 (en) * 1991-04-01 1992-10-15 The Salk Institute Biotechnology/Industrial Associates, Inc. Genes which influence pichia proteolytic activity, and uses therefor
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5834262A (en) * 1992-01-06 1998-11-10 E. I. Du Pont De Nemours And Company Oxidation of glycolic acid to glyoxylic acid using a microbial cell transformant as catalyst
DE69303943T2 (de) * 1992-02-28 1997-01-30 Suntory Ltd Neuer Vektor, einen von Methanol und/oder Glyzerin induzierbaren Promotor enthaltend
US5708141A (en) * 1992-05-11 1998-01-13 Corvas International, Inc. Neutrophil inhibitors
AU6355494A (en) * 1993-03-03 1994-09-26 E.I. Du Pont De Nemours And Company Production of glycolate oxidase in methylotrophic yeast
CA2155330C (en) * 1993-03-08 2002-01-29 Kathryn J. Elliott Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
AU6415594A (en) * 1993-03-25 1994-10-11 Biosource Genetics Corporation (pichia pastoris) alcohol oxidase (zza1) and (zza2)
US5849895A (en) * 1993-04-20 1998-12-15 Sibia Neurosciences, Inc. Human N-methyl-D-aspartate receptor subunits, nucleic acids encoding same and uses therefor
US6001581A (en) 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US5912122A (en) * 1993-06-04 1999-06-15 Sibia Neurosciences, Inc. Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6
US5521297A (en) 1993-06-04 1996-05-28 Salk Institute Biotechnology/Industrial Associates Nucleic acids encoding human metabotropic glutamate receptors
US6638905B2 (en) * 1993-06-18 2003-10-28 The Salk Institute For Biological Studies Cloning and recombinant production of CFR receptor(s)
US6495343B1 (en) 1993-06-18 2002-12-17 The Salk Institute For Biological Studies Cloning and recombinant production of CRF receptor(s)
AU1091595A (en) * 1993-11-08 1995-05-29 Salk Institute Biotechnology/Industrial Associates, Inc., The Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
US5453161A (en) * 1993-11-12 1995-09-26 Enichem S.P.A. Polyamic acid to polyimide conversion by microwave heating
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
JP3811186B2 (ja) * 1994-10-18 2006-08-16 コーバス インターナショナル， インコーポレイテッド 線虫から抽出されるセリンプロテアーゼ阻害剤および抗凝固性タンパク質
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6485967B1 (en) 1995-06-07 2002-11-26 Merck & Co., Inc. Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid
AU712650B2 (en) * 1995-11-09 1999-11-11 Zymogenetics Inc. Compositions and methods for producing heterologous polypeptides in pichia methanolica
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
DE69834533T2 (de) 1997-10-01 2006-10-12 Dsm Ip Assets B.V. Verfahren zur Protein-Herstellung
CA2307016A1 (en) * 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
US7393632B2 (en) 1999-12-10 2008-07-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
DE69927375T2 (de) * 1998-07-06 2006-06-22 Tosoh Corp., Shinnanyo Fusionsprotein mit direkter verknüpfung des il-6-rezeptors mit il-6
JP4524816B2 (ja) * 1998-07-06 2010-08-18 東ソー株式会社 Ｉｌ−６レセプター・ｉｌ−６直結融合蛋白質
AU5584999A (en) * 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6780615B1 (en) 1998-12-31 2004-08-24 Genway Biotech Inc. Production of recombinant monellin using methylotrophic yeast expression system
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
EP2336148A1 (en) 1999-03-02 2011-06-22 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
US7504253B2 (en) * 1999-06-11 2009-03-17 The Burnham Institute For Medical Research Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
HK1046016B (en) 1999-11-12 2009-02-27 Fibrogen, Inc. Recombinant gelatin in vaccines
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
WO2001077351A1 (en) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vectors and methods for dual protein expression in pichia pastoris and escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
WO2002000879A2 (en) * 2000-06-28 2002-01-03 Glycofi, Inc. Methods for producing modified glycoproteins
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
MXPA02012688A (es) 2000-06-30 2004-09-10 Flanders Interuniversity Inst Modificacion de glicosilacion de proteina en pichia pastoris.
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6699691B2 (en) 2001-04-05 2004-03-02 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
WO2002095055A2 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
US20060088834A1 (en) * 2002-04-15 2006-04-27 Gretchen Bain Matrix analysis of gene expression in cells (magec)
IL165717A0 (en) 2002-06-26 2006-01-15 Flanders Interuniversity Inst A strain of methylotrophic yeast for producing proteins
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
US7118901B2 (en) * 2002-12-18 2006-10-10 Roche Diagnostics Operations, Inc. Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
ATE387494T1 (de) * 2002-12-20 2008-03-15 Hoffmann La Roche Hitzelabile desoxyribonuklease i-varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
ES2366830T3 (es) 2003-08-25 2011-10-25 Funzyme Biotechnologies Sa Nuevas proteínas fúngicas y ácidos nucelicos que codifican las mismas.
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
JP2007512838A (ja) 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法
JP4411192B2 (ja) * 2003-12-05 2010-02-10 エフ．ホフマン−ラ ロシュ アーゲー 組換えで発現されるカルボキシペプチダーゼｂおよびその精製
PT1926749E (pt) * 2005-09-14 2011-09-29 Sanofi Aventis Deutschland Clivagem de precursores de insulinas por uma variante de tripsina
AT504588B1 (de) * 2006-11-16 2008-08-15 Univ Graz Tech Nukleinsäure-promotor
KR20100120708A (ko) 2008-03-03 2010-11-16 아보트 러보러터리즈 효모를 형질전환시키는 방법
KR20110101198A (ko) 2008-12-12 2011-09-15 이 아이 듀폰 디 네모아 앤드 캄파니 재생 가능 자원으로부터의 선형 다이카르복실산의 제조 방법
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
CA2784944A1 (en) 2009-12-21 2011-06-30 Centre Hospitalier Universitaire Vaudois Synergic action of a prolyl protease and tripeptidyl proteases
DK2710114T3 (da) 2011-05-20 2021-12-06 H Lundbeck As Fremstilling med høj renhed af proteiner med multi-underenheder såsom antistoffer i transformerede mikrober såsom pichia pastoris
MX355890B (es) 2011-08-19 2018-05-03 Alderbio Holdings Llc Estrategia de múltiples copias para producción de alto título y alta pureza de proteínas de multi-subunidades tales como anticuerpos en microbios transformados tal como pichia pastoris.
JP6113736B2 (ja) 2011-10-07 2017-04-12 ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ 調節可能なプロモーター
JP6698352B2 (ja) 2013-03-15 2020-05-27 ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ 構成的プロモーター
JP6472786B2 (ja) 2013-03-15 2019-02-20 アルダー・バイオファーマシューティカルズ・インコーポレーテッド 抗体の精製及び純度のモニタリング
MX384491B (es) 2013-03-15 2025-03-14 H Lundbeck As Cambio de temperatura para una expresion con mayor rendimiento de polipeptidos en levaduras y otras celulas transformadas.
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
JP6594293B2 (ja) 2013-03-21 2019-10-23 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション 三重らせんタンパク質の精製
EP2999712B1 (en) 2013-04-30 2018-11-21 Donald Danforth Plant Science Center Antifungal plant proteins, peptides, and methods of use
KR102384622B1 (ko) 2013-09-09 2022-04-11 에보닉 오퍼레이션스 게엠베하 변형된 세균 콜라겐-유사 단백질
WO2016120764A1 (en) 2015-01-26 2016-08-04 Centre Hospitalier Universitaire Vaudois (Chuv) Aspergillus oryzae prolyl endopeptidases and use thereof in degradation of polypeptides
US20220220491A1 (en) * 2019-12-19 2022-07-14 Atatürk Üniversitesi Bilimsel Arastirma Projeleri Birimi A method for recombinant protein production

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE54046B1 (en) * 1981-08-25 1989-05-24 Celltech Ltd Expression vectors
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
DE3381090D1 (de) * 1982-05-19 1990-02-15 Gist Brocades Nv Klonierungssystem fuer kluyveromyce spezies.
WO1984001153A1 (en) * 1982-09-15 1984-03-29 Collaborative Res Inc Production of interferon in yeast by use of invertase promoter
EP0423890A3 (en) * 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia

Also Published As

Publication number Publication date
DD254211A5 (de) 1988-02-17
AU572002B2 (en) 1988-04-28
KR930001117B1 (ko) 1993-02-18
CN1011243B (zh) 1991-01-16
NZ213842A (en) 1991-11-26
GR852611B (cs) 1986-03-04
FI854142A0 (fi) 1985-10-23
YU169585A (en) 1991-04-30
AU4875285A (en) 1986-06-12
ES8609458A1 (es) 1986-09-01
CN85109718A (zh) 1986-08-20
EP0183071A2 (en) 1986-06-04
FI94427B (fi) 1995-05-31
ES548307A0 (es) 1986-09-01
HU205627B (en) 1992-05-28
FI94427C (fi) 1995-09-11
US4855231A (en) 1989-08-08
CA1340733C (en) 1999-09-14
DE3586889D1 (de) 1993-01-21
EP0183071A3 (en) 1987-09-23
JP2552807B2 (ja) 1996-11-13
HUT38678A (en) 1986-06-30
JPH08173161A (ja) 1996-07-09
JPH0698789A (ja) 1994-04-12
JP2502508B2 (ja) 1996-05-29
IE930804L (en) 1986-04-30
ATE83263T1 (de) 1992-12-15
KR870003197A (ko) 1987-04-15
JPH06233686A (ja) 1994-08-23
JPH08196275A (ja) 1996-08-06
NO178076B (no) 1995-10-09
PL256012A1 (en) 1986-09-23
FI854142L (fi) 1986-05-01
YU46695B (sh) 1994-04-05
NO178076C (no) 1996-01-17
EP0183071B1 (en) 1992-12-09
PT81399A (en) 1985-11-01
JP2552808B2 (ja) 1996-11-13
DK496385D0 (da) 1985-10-29
NO854312L (no) 1986-05-02
EP0483115A2 (en) 1992-04-29
PT81399B (pt) 1987-11-11
JPS61173781A (ja) 1986-08-05
IL76765A (en) 1991-06-30
PL158965B1 (pl) 1992-10-30
AR242988A1 (es) 1993-06-30
DE3586889T2 (de) 1993-04-15
EP0483115A3 (en) 1992-05-06

Similar Documents

Publication Publication Date Title
DK496385A (da) Reguleringsregion for heterolog genekspression i gaer
ZA858181B (en) Regulatory region for heterologous gene expression in yeast
AU3824285A (en) Exogenous gene expression insect cells
DK479685D0 (da) Stol
GB2165087B (en) Improved operation of microbial fuel cells
EP0211151A3 (en) Procedure for controlling the order of editing cells in a spreadsheet
IT1185721B (it) Regolatore di depressione
FI872470A7 (fi) Galaktoosin säätelemän geeniekspression indusointi hiivassa.
DE3480006D1 (en) Improvements in the expression of newly introduced genes in yeast cells
DK179785D0 (da) Fermenteringsproces
IT1185032B (it) Riscaldatore di combustibile
DK535485A (da) Indstillelig stol
EP0252737A3 (en) Improved strains of yeast for the expression of heterologous genes
GB8503248D0 (en) Culturing cells
EP0163472A3 (en) Medium for electrophoresis
GR80044B (en) Yeast coppermetallothionein gene
IL83435A0 (en) Gene expression in yeast
DK153145C (da) 1alfa-hydroxyvitamin d2 analoge
FI893942A7 (fi) Faktoria säätävä geeniekspressio
IE842022L (en) Yeast gene
ZA846096B (en) Yeast copper-metallothionein gene
KR860002144U (ko) 배양액 순환 재배조
AT385573B (de) Gesteuerter durchflussregler
GB8511993D0 (en) Culture of normal epithelial cells
GB8418511D0 (en) Regulation of gene expression

Legal Events

Date Code Title Description
AHB Application shelved due to non-payment