DK2262901T3 - ORGANISMS PRODUCING PRIMARY ALCOHOL - Google Patents
ORGANISMS PRODUCING PRIMARY ALCOHOL Download PDFInfo
- Publication number
- DK2262901T3 DK2262901T3 DK09717987.3T DK09717987T DK2262901T3 DK 2262901 T3 DK2262901 T3 DK 2262901T3 DK 09717987 T DK09717987 T DK 09717987T DK 2262901 T3 DK2262901 T3 DK 2262901T3
- Authority
- DK
- Denmark
- Prior art keywords
- coa
- growth
- production
- acyl
- gene
- Prior art date
Links
- 150000003138 primary alcohols Chemical class 0.000 title claims description 105
- 102000004190 Enzymes Human genes 0.000 claims description 156
- 108090000790 Enzymes Proteins 0.000 claims description 156
- 230000037361 pathway Effects 0.000 claims description 152
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 claims description 151
- 238000000034 method Methods 0.000 claims description 135
- 230000000813 microbial effect Effects 0.000 claims description 117
- 230000004136 fatty acid synthesis Effects 0.000 claims description 108
- 230000000694 effects Effects 0.000 claims description 106
- LTYOQGRJFJAKNA-KKIMTKSISA-N Malonyl CoA Natural products S(C(=O)CC(=O)O)CCNC(=O)CCNC(=O)[C@@H](O)C(CO[P@](=O)(O[P@](=O)(OC[C@H]1[C@@H](OP(=O)(O)O)[C@@H](O)[C@@H](n2c3ncnc(N)c3nc2)O1)O)O)(C)C LTYOQGRJFJAKNA-KKIMTKSISA-N 0.000 claims description 102
- LTYOQGRJFJAKNA-DVVLENMVSA-N malonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LTYOQGRJFJAKNA-DVVLENMVSA-N 0.000 claims description 102
- 150000007523 nucleic acids Chemical class 0.000 claims description 70
- 108020004707 nucleic acids Proteins 0.000 claims description 67
- 102000039446 nucleic acids Human genes 0.000 claims description 67
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims description 40
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 40
- 210000000172 cytosol Anatomy 0.000 claims description 36
- 108090001018 hexadecanal dehydrogenase (acylating) Proteins 0.000 claims description 31
- 108010087894 Fatty acid desaturases Proteins 0.000 claims description 28
- 102000009114 Fatty acid desaturases Human genes 0.000 claims description 27
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 claims description 26
- 102000052553 3-Hydroxyacyl CoA Dehydrogenase Human genes 0.000 claims description 22
- 108700020831 3-Hydroxyacyl-CoA Dehydrogenase Proteins 0.000 claims description 22
- 108010023922 Enoyl-CoA hydratase Proteins 0.000 claims description 22
- 102000011426 Enoyl-CoA hydratase Human genes 0.000 claims description 21
- 230000008569 process Effects 0.000 claims description 21
- 108060008225 Thiolase Proteins 0.000 claims description 20
- 102000002932 Thiolase Human genes 0.000 claims description 20
- 230000009467 reduction Effects 0.000 claims description 19
- 238000012258 culturing Methods 0.000 claims description 16
- 108700016155 Acyl transferases Proteins 0.000 claims description 12
- 102000057234 Acyl transferases Human genes 0.000 claims description 12
- 150000002191 fatty alcohols Chemical class 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 9
- MWKFXSUHUHTGQN-UHFFFAOYSA-N decan-1-ol Chemical compound CCCCCCCCCCO MWKFXSUHUHTGQN-UHFFFAOYSA-N 0.000 claims description 8
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 claims description 8
- BBMCTIGTTCKYKF-UHFFFAOYSA-N 1-heptanol Chemical compound CCCCCCCO BBMCTIGTTCKYKF-UHFFFAOYSA-N 0.000 claims description 6
- 125000002252 acyl group Chemical group 0.000 claims description 6
- HLZKNKRTKFSKGZ-UHFFFAOYSA-N tetradecan-1-ol Chemical compound CCCCCCCCCCCCCCO HLZKNKRTKFSKGZ-UHFFFAOYSA-N 0.000 claims description 6
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- ZWRUINPWMLAQRD-UHFFFAOYSA-N nonan-1-ol Chemical compound CCCCCCCCCO ZWRUINPWMLAQRD-UHFFFAOYSA-N 0.000 claims 4
- 238000006722 reduction reaction Methods 0.000 claims 4
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 claims 2
- 238000002425 crystallisation Methods 0.000 claims 1
- 230000008025 crystallization Effects 0.000 claims 1
- 238000004821 distillation Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 description 319
- 108090000623 proteins and genes Proteins 0.000 description 292
- 230000012010 growth Effects 0.000 description 253
- 239000000047 product Substances 0.000 description 142
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 124
- 238000006243 chemical reaction Methods 0.000 description 120
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 112
- 230000002503 metabolic effect Effects 0.000 description 94
- 230000015572 biosynthetic process Effects 0.000 description 89
- 238000013461 design Methods 0.000 description 89
- 241000588724 Escherichia coli Species 0.000 description 66
- 210000004027 cell Anatomy 0.000 description 63
- 244000005700 microbiome Species 0.000 description 54
- 241000894007 species Species 0.000 description 53
- 230000004907 flux Effects 0.000 description 50
- 238000000855 fermentation Methods 0.000 description 42
- 230000004151 fermentation Effects 0.000 description 42
- 230000004048 modification Effects 0.000 description 42
- 238000012986 modification Methods 0.000 description 42
- 230000014509 gene expression Effects 0.000 description 38
- 229910052799 carbon Inorganic materials 0.000 description 36
- 239000000243 solution Substances 0.000 description 36
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 35
- 210000003470 mitochondria Anatomy 0.000 description 35
- 230000002438 mitochondrial effect Effects 0.000 description 35
- 230000003044 adaptive effect Effects 0.000 description 34
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 32
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 32
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 32
- 239000008103 glucose Substances 0.000 description 31
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 30
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 30
- 101710088194 Dehydrogenase Proteins 0.000 description 29
- 239000002028 Biomass Substances 0.000 description 27
- 108010049926 Acetate-CoA ligase Proteins 0.000 description 26
- 102100035709 Acetyl-coenzyme A synthetase, cytoplasmic Human genes 0.000 description 26
- 239000000758 substrate Substances 0.000 description 26
- 150000001298 alcohols Chemical class 0.000 description 25
- 238000010168 coupling process Methods 0.000 description 25
- 238000005859 coupling reaction Methods 0.000 description 25
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 24
- 230000001086 cytosolic effect Effects 0.000 description 24
- 238000000126 in silico method Methods 0.000 description 24
- 239000001301 oxygen Substances 0.000 description 24
- 229910052760 oxygen Inorganic materials 0.000 description 24
- 230000008878 coupling Effects 0.000 description 23
- 238000012217 deletion Methods 0.000 description 23
- 230000037430 deletion Effects 0.000 description 23
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 23
- 230000001105 regulatory effect Effects 0.000 description 23
- 230000004077 genetic alteration Effects 0.000 description 22
- 230000006680 metabolic alteration Effects 0.000 description 22
- 108010011939 Pyruvate Decarboxylase Proteins 0.000 description 21
- 238000013459 approach Methods 0.000 description 21
- 239000002609 medium Substances 0.000 description 21
- 238000006241 metabolic reaction Methods 0.000 description 21
- 231100000118 genetic alteration Toxicity 0.000 description 20
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 20
- 238000005457 optimization Methods 0.000 description 20
- 238000012224 gene deletion Methods 0.000 description 19
- 230000006870 function Effects 0.000 description 18
- 230000004190 glucose uptake Effects 0.000 description 17
- 230000006872 improvement Effects 0.000 description 17
- 102000004169 proteins and genes Human genes 0.000 description 17
- 238000004088 simulation Methods 0.000 description 17
- 239000006227 byproduct Substances 0.000 description 16
- 239000000376 reactant Substances 0.000 description 16
- 108010081577 aldehyde dehydrogenase (NAD(P)+) Proteins 0.000 description 15
- 235000014113 dietary fatty acids Nutrition 0.000 description 15
- 229930195729 fatty acid Natural products 0.000 description 15
- 239000000194 fatty acid Substances 0.000 description 15
- 230000028327 secretion Effects 0.000 description 15
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 14
- 230000003698 anagen phase Effects 0.000 description 14
- 230000010261 cell growth Effects 0.000 description 14
- 150000004665 fatty acids Chemical class 0.000 description 14
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 13
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 13
- 102000004316 Oxidoreductases Human genes 0.000 description 13
- 108090000854 Oxidoreductases Proteins 0.000 description 13
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 13
- 238000010924 continuous production Methods 0.000 description 13
- 230000002255 enzymatic effect Effects 0.000 description 13
- 108010008221 formate C-acetyltransferase Proteins 0.000 description 13
- 239000007788 liquid Substances 0.000 description 13
- 230000004060 metabolic process Effects 0.000 description 13
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 12
- 244000025670 Eleusine indica Species 0.000 description 12
- 238000010276 construction Methods 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 12
- 230000035772 mutation Effects 0.000 description 12
- 239000012071 phase Substances 0.000 description 12
- 229920001184 polypeptide Polymers 0.000 description 12
- 102000004196 processed proteins & peptides Human genes 0.000 description 12
- 108090000765 processed proteins & peptides Proteins 0.000 description 12
- 239000013604 expression vector Substances 0.000 description 11
- 239000000203 mixture Substances 0.000 description 11
- 230000003287 optical effect Effects 0.000 description 11
- 108010036781 Fumarate Hydratase Proteins 0.000 description 10
- 102100036160 Fumarate hydratase, mitochondrial Human genes 0.000 description 10
- 241000233866 Fungi Species 0.000 description 10
- 108010086428 NADH Dehydrogenase Proteins 0.000 description 10
- 102000006746 NADH Dehydrogenase Human genes 0.000 description 10
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 10
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 10
- 238000007792 addition Methods 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 10
- 238000006073 displacement reaction Methods 0.000 description 10
- 230000001965 increasing effect Effects 0.000 description 10
- 230000037353 metabolic pathway Effects 0.000 description 10
- 239000013612 plasmid Substances 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 238000003786 synthesis reaction Methods 0.000 description 10
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 9
- 102000016901 Glutamate dehydrogenase Human genes 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 230000008901 benefit Effects 0.000 description 9
- 230000003197 catalytic effect Effects 0.000 description 9
- 238000000205 computational method Methods 0.000 description 9
- 230000001419 dependent effect Effects 0.000 description 9
- 239000012737 fresh medium Substances 0.000 description 9
- 238000004128 high performance liquid chromatography Methods 0.000 description 9
- 238000012269 metabolic engineering Methods 0.000 description 9
- -1 octanol and hexanol Chemical class 0.000 description 9
- 230000002829 reductive effect Effects 0.000 description 9
- 238000000926 separation method Methods 0.000 description 9
- 238000012546 transfer Methods 0.000 description 9
- 230000009466 transformation Effects 0.000 description 9
- 239000013598 vector Substances 0.000 description 9
- 241000193401 Clostridium acetobutylicum Species 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 8
- 241001646716 Escherichia coli K-12 Species 0.000 description 8
- 230000005526 G1 to G0 transition Effects 0.000 description 8
- 230000001851 biosynthetic effect Effects 0.000 description 8
- 230000002950 deficient Effects 0.000 description 8
- 230000001939 inductive effect Effects 0.000 description 8
- 230000000670 limiting effect Effects 0.000 description 8
- 150000007524 organic acids Chemical class 0.000 description 8
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 7
- 240000005384 Rhizopus oryzae Species 0.000 description 7
- 238000009825 accumulation Methods 0.000 description 7
- 230000004075 alteration Effects 0.000 description 7
- 230000003115 biocidal effect Effects 0.000 description 7
- 238000004422 calculation algorithm Methods 0.000 description 7
- 230000002349 favourable effect Effects 0.000 description 7
- 239000007789 gas Substances 0.000 description 7
- 238000010348 incorporation Methods 0.000 description 7
- 239000000543 intermediate Substances 0.000 description 7
- 108010062385 long-chain-alcohol O-fatty-acyltransferase Proteins 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 239000002207 metabolite Substances 0.000 description 7
- 239000001993 wax Substances 0.000 description 7
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 6
- 108020002908 Epoxide hydrolase Proteins 0.000 description 6
- 102100035428 Formiminotransferase N-terminal subdomain-containing protein Human genes 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 101000877728 Homo sapiens Formiminotransferase N-terminal subdomain-containing protein Proteins 0.000 description 6
- 241000235058 Komagataella pastoris Species 0.000 description 6
- 101710185553 Malate dehydrogenase, cytoplasmic Proteins 0.000 description 6
- 102100026475 Malate dehydrogenase, cytoplasmic Human genes 0.000 description 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
- 241000235070 Saccharomyces Species 0.000 description 6
- 150000001413 amino acids Chemical group 0.000 description 6
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 6
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 230000001747 exhibiting effect Effects 0.000 description 6
- 150000002194 fatty esters Chemical class 0.000 description 6
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 6
- 238000012239 gene modification Methods 0.000 description 6
- 230000005017 genetic modification Effects 0.000 description 6
- 235000013617 genetically modified food Nutrition 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 238000012261 overproduction Methods 0.000 description 6
- 230000003647 oxidation Effects 0.000 description 6
- 238000007254 oxidation reaction Methods 0.000 description 6
- 230000037039 plant physiology Effects 0.000 description 6
- 230000002035 prolonged effect Effects 0.000 description 6
- 230000008685 targeting Effects 0.000 description 6
- 102000004567 6-phosphogluconate dehydrogenase Human genes 0.000 description 5
- 108020001657 6-phosphogluconate dehydrogenase Proteins 0.000 description 5
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 5
- 102000005486 Epoxide hydrolase Human genes 0.000 description 5
- 102100031132 Glucose-6-phosphate isomerase Human genes 0.000 description 5
- 108010070600 Glucose-6-phosphate isomerase Proteins 0.000 description 5
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 5
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 5
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 5
- 241000589540 Pseudomonas fluorescens Species 0.000 description 5
- 241000589776 Pseudomonas putida Species 0.000 description 5
- 235000013752 Rhizopus oryzae Nutrition 0.000 description 5
- 239000004164 Wax ester Substances 0.000 description 5
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 5
- 230000003190 augmentative effect Effects 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 230000008030 elimination Effects 0.000 description 5
- 238000003379 elimination reaction Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000003209 gene knockout Methods 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 238000010353 genetic engineering Methods 0.000 description 5
- 230000001976 improved effect Effects 0.000 description 5
- 239000002054 inoculum Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 230000001590 oxidative effect Effects 0.000 description 5
- 239000002243 precursor Substances 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 230000014616 translation Effects 0.000 description 5
- 230000032258 transport Effects 0.000 description 5
- 235000019386 wax ester Nutrition 0.000 description 5
- 108010092060 Acetate kinase Proteins 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 4
- 241000228245 Aspergillus niger Species 0.000 description 4
- 241001465318 Aspergillus terreus Species 0.000 description 4
- 241000186226 Corynebacterium glutamicum Species 0.000 description 4
- 241000252867 Cupriavidus metallidurans Species 0.000 description 4
- 241000195619 Euglena gracilis Species 0.000 description 4
- 108090000698 Formate Dehydrogenases Proteins 0.000 description 4
- 102000000587 Glycerolphosphate Dehydrogenase Human genes 0.000 description 4
- 108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 description 4
- 101000780205 Homo sapiens Long-chain-fatty-acid-CoA ligase 5 Proteins 0.000 description 4
- 101000780202 Homo sapiens Long-chain-fatty-acid-CoA ligase 6 Proteins 0.000 description 4
- 241001138401 Kluyveromyces lactis Species 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 108700023175 Phosphate acetyltransferases Proteins 0.000 description 4
- 244000253911 Saccharomyces fragilis Species 0.000 description 4
- 244000044822 Simmondsia californica Species 0.000 description 4
- 235000004433 Simmondsia californica Nutrition 0.000 description 4
- 108010065064 acetaldehyde dehydrogenase (acylating) Proteins 0.000 description 4
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 230000002759 chromosomal effect Effects 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 239000012228 culture supernatant Substances 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 150000002185 fatty acyl-CoAs Chemical class 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 150000002430 hydrocarbons Chemical class 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- 238000012423 maintenance Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 235000005985 organic acids Nutrition 0.000 description 4
- 230000002018 overexpression Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 238000000844 transformation Methods 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- TZBGSHAFWLGWBO-ABLWVSNPSA-N (2s)-2-[[4-[(2-amino-4-oxo-5,6,7,8-tetrahydro-1h-pteridin-6-yl)methylamino]benzoyl]amino]-5-methoxy-5-oxopentanoic acid Chemical compound C1=CC(C(=O)N[C@@H](CCC(=O)OC)C(O)=O)=CC=C1NCC1NC(C(=O)NC(N)=N2)=C2NC1 TZBGSHAFWLGWBO-ABLWVSNPSA-N 0.000 description 3
- 241000948980 Actinobacillus succinogenes Species 0.000 description 3
- 101710146995 Acyl carrier protein Proteins 0.000 description 3
- 108010080691 Alcohol O-acetyltransferase Proteins 0.000 description 3
- 241000722954 Anaerobiospirillum succiniciproducens Species 0.000 description 3
- 101100388296 Arabidopsis thaliana DTX51 gene Proteins 0.000 description 3
- 241000203069 Archaea Species 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- 241000206602 Eukaryota Species 0.000 description 3
- 241000589232 Gluconobacter oxydans Species 0.000 description 3
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 3
- 240000006024 Lactobacillus plantarum Species 0.000 description 3
- 102100034337 Long-chain-fatty-acid-CoA ligase 6 Human genes 0.000 description 3
- 102000016387 Pancreatic elastase Human genes 0.000 description 3
- 108010067372 Pancreatic elastase Proteins 0.000 description 3
- 102000013009 Pyruvate Kinase Human genes 0.000 description 3
- 108020005115 Pyruvate Kinase Proteins 0.000 description 3
- 108010042687 Pyruvate Oxidase Proteins 0.000 description 3
- 241001148115 Rhizobium etli Species 0.000 description 3
- 101100215626 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADP1 gene Proteins 0.000 description 3
- 101100082596 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDC5 gene Proteins 0.000 description 3
- 101100519200 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDC6 gene Proteins 0.000 description 3
- 235000018368 Saccharomyces fragilis Nutrition 0.000 description 3
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 3
- 244000057717 Streptococcus lactis Species 0.000 description 3
- 241000187432 Streptomyces coelicolor Species 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- 241000588902 Zymomonas mobilis Species 0.000 description 3
- 241000029538 [Mannheimia] succiniciproducens Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000001299 aldehydes Chemical class 0.000 description 3
- 230000009604 anaerobic growth Effects 0.000 description 3
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 3
- 239000012298 atmosphere Substances 0.000 description 3
- 230000002238 attenuated effect Effects 0.000 description 3
- 238000010923 batch production Methods 0.000 description 3
- 238000005842 biochemical reaction Methods 0.000 description 3
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 150000002148 esters Chemical class 0.000 description 3
- 101150069125 fadB gene Proteins 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 239000000446 fuel Substances 0.000 description 3
- 238000002309 gasification Methods 0.000 description 3
- 238000002744 homologous recombination Methods 0.000 description 3
- 230000006801 homologous recombination Effects 0.000 description 3
- 229930195733 hydrocarbon Natural products 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 229940031154 kluyveromyces marxianus Drugs 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 3
- 238000000424 optical density measurement Methods 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 230000004108 pentose phosphate pathway Effects 0.000 description 3
- 230000000737 periodic effect Effects 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- 230000008672 reprogramming Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 230000002459 sustained effect Effects 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 230000009614 wildtype growth Effects 0.000 description 3
- 102000001762 6-phosphogluconolactonase Human genes 0.000 description 2
- 241000589291 Acinetobacter Species 0.000 description 2
- 241000588625 Acinetobacter sp. Species 0.000 description 2
- 108700028369 Alleles Proteins 0.000 description 2
- 241000186570 Clostridium kluyveri Species 0.000 description 2
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 description 2
- 108010051219 Cre recombinase Proteins 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 2
- 108010001348 Diacylglycerol O-acyltransferase Proteins 0.000 description 2
- 102000002148 Diacylglycerol O-acyltransferase Human genes 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 101100350701 Escherichia coli (strain K12) paaC gene Proteins 0.000 description 2
- 101710082056 Ethanol acetyltransferase 1 Proteins 0.000 description 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 2
- 239000005977 Ethylene Substances 0.000 description 2
- 108091029865 Exogenous DNA Chemical group 0.000 description 2
- 108060002716 Exonuclease Proteins 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 description 2
- 102100025413 Formyltetrahydrofolate synthetase Human genes 0.000 description 2
- 102100033295 Glial cell line-derived neurotrophic factor Human genes 0.000 description 2
- 101150069554 HIS4 gene Proteins 0.000 description 2
- 241000631636 Ishige Species 0.000 description 2
- 108010044467 Isoenzymes Proteins 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 102000002568 Multienzyme Complexes Human genes 0.000 description 2
- 108010093369 Multienzyme Complexes Proteins 0.000 description 2
- 101150040763 PDC3 gene Proteins 0.000 description 2
- 101100463818 Pseudomonas oleovorans phaC1 gene Proteins 0.000 description 2
- 108010091086 Recombinases Proteins 0.000 description 2
- 102000018120 Recombinases Human genes 0.000 description 2
- 101100297400 Rhizobium meliloti (strain 1021) phaAB gene Proteins 0.000 description 2
- 244000117054 Rungia klossii Species 0.000 description 2
- 235000002492 Rungia klossii Nutrition 0.000 description 2
- 101100433695 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) AAD16 gene Proteins 0.000 description 2
- 101100434411 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADH1 gene Proteins 0.000 description 2
- 101100054940 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADH3 gene Proteins 0.000 description 2
- 101100054948 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADH4 gene Proteins 0.000 description 2
- 101100054950 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADH5 gene Proteins 0.000 description 2
- 101100001024 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADH6 gene Proteins 0.000 description 2
- 101100001028 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADH7 gene Proteins 0.000 description 2
- 101100493787 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) BDH2 gene Proteins 0.000 description 2
- 101100393304 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GPD1 gene Proteins 0.000 description 2
- 101100393309 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GPD2 gene Proteins 0.000 description 2
- 101100069218 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GUT2 gene Proteins 0.000 description 2
- 101100290494 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MDH2 gene Proteins 0.000 description 2
- 101100348117 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) NDE1 gene Proteins 0.000 description 2
- 101100348120 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) NDE2 gene Proteins 0.000 description 2
- 101100459997 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) NDI1 gene Proteins 0.000 description 2
- 101100082579 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDC1 gene Proteins 0.000 description 2
- 101000636911 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial Proteins 0.000 description 2
- 101100502339 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SFA1 gene Proteins 0.000 description 2
- 102000012479 Serine Proteases Human genes 0.000 description 2
- 108010022999 Serine Proteases Proteins 0.000 description 2
- 235000014897 Streptococcus lactis Nutrition 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000001588 bifunctional effect Effects 0.000 description 2
- CRFNGMNYKDXRTN-CITAKDKDSA-N butyryl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 CRFNGMNYKDXRTN-CITAKDKDSA-N 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 230000019522 cellular metabolic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003245 coal Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000001447 compensatory effect Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000010205 computational analysis Methods 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 description 2
- 229910001882 dioxygen Inorganic materials 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000003828 downregulation Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 101150004992 fadA gene Proteins 0.000 description 2
- 101150092019 fadJ gene Proteins 0.000 description 2
- 235000019387 fatty acid methyl ester Nutrition 0.000 description 2
- 102000005970 fatty acyl-CoA reductase Human genes 0.000 description 2
- 150000002192 fatty aldehydes Chemical class 0.000 description 2
- 238000012246 gene addition Methods 0.000 description 2
- 238000010363 gene targeting Methods 0.000 description 2
- 235000003869 genetically modified organism Nutrition 0.000 description 2
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 2
- 230000034659 glycolysis Effects 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000006151 minimal media Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 101150046540 phaA gene Proteins 0.000 description 2
- 108010022393 phosphogluconate dehydratase Proteins 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 239000010908 plant waste Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 238000002708 random mutagenesis Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 102000037983 regulatory factors Human genes 0.000 description 2
- 108091008025 regulatory factors Proteins 0.000 description 2
- 230000000284 resting effect Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- ZKVGHXMOFBDFGA-MJQNIGQHSA-N s-[2-[3-[[(2r)-4-[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] 2-oxohexanethioate Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C(=O)CCCC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZKVGHXMOFBDFGA-MJQNIGQHSA-N 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 230000005030 transcription termination Effects 0.000 description 2
- 238000012070 whole genome sequencing analysis Methods 0.000 description 2
- MJYQFWSXKFLTAY-OVEQLNGDSA-N (2r,3r)-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]butane-1,4-diol;(2r,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O.C1=C(O)C(OC)=CC(C[C@@H](CO)[C@H](CO)CC=2C=C(OC)C(O)=CC=2)=C1 MJYQFWSXKFLTAY-OVEQLNGDSA-N 0.000 description 1
- 102100021834 3-hydroxyacyl-CoA dehydrogenase Human genes 0.000 description 1
- 102100026105 3-ketoacyl-CoA thiolase, mitochondrial Human genes 0.000 description 1
- MEANFMOQMXYMCT-OLZOCXBDSA-N 5,10-methenyltetrahydrofolic acid Chemical compound C([C@H]1CNC2=C([N+]1=C1)C(=O)N=C(N2)N)N1C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C([O-])=O)C=C1 MEANFMOQMXYMCT-OLZOCXBDSA-N 0.000 description 1
- 101150050888 ACS2 gene Proteins 0.000 description 1
- 108090001107 Acetate-CoA ligase (ADP-forming) Proteins 0.000 description 1
- 241001468161 Acetobacterium Species 0.000 description 1
- 108010003902 Acetyl-CoA C-acyltransferase Proteins 0.000 description 1
- 108010016219 Acetyl-CoA carboxylase Proteins 0.000 description 1
- 102000000452 Acetyl-CoA carboxylase Human genes 0.000 description 1
- 241000588624 Acinetobacter calcoaceticus Species 0.000 description 1
- 108700037654 Acyl carrier protein (ACP) Proteins 0.000 description 1
- 102000048456 Acyl carrier protein (ACP) Human genes 0.000 description 1
- 241000611270 Alcanivorax borkumensis Species 0.000 description 1
- 108030004487 Alcohol-forming fatty acyl-CoA reductases Proteins 0.000 description 1
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 description 1
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 description 1
- 102000016912 Aldehyde Reductase Human genes 0.000 description 1
- 108010053754 Aldehyde reductase Proteins 0.000 description 1
- 102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 description 1
- 101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 108010006591 Apoenzymes Proteins 0.000 description 1
- 101100433502 Arabidopsis thaliana ACS7 gene Proteins 0.000 description 1
- 101001074429 Bacillus subtilis (strain 168) Polyketide biosynthesis acyltransferase homolog PksD Proteins 0.000 description 1
- 101100351124 Bacillus subtilis (strain 168) pckA gene Proteins 0.000 description 1
- 101100098786 Bacillus subtilis (strain 168) tapA gene Proteins 0.000 description 1
- 101000936617 Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) Polyketide biosynthesis acyltransferase homolog BaeD Proteins 0.000 description 1
- 108010018763 Biotin carboxylase Proteins 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 101100280051 Brucella abortus biovar 1 (strain 9-941) eryH gene Proteins 0.000 description 1
- 108010068197 Butyryl-CoA Dehydrogenase Proteins 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 101100351264 Candida albicans (strain SC5314 / ATCC MYA-2876) PDC11 gene Proteins 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 241000193454 Clostridium beijerinckii Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 102000007528 DNA Polymerase III Human genes 0.000 description 1
- 108010071146 DNA Polymerase III Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 101710088335 Diacylglycerol acyltransferase/mycolyltransferase Ag85A Proteins 0.000 description 1
- 101710088334 Diacylglycerol acyltransferase/mycolyltransferase Ag85B Proteins 0.000 description 1
- 101710088427 Diacylglycerol acyltransferase/mycolyltransferase Ag85C Proteins 0.000 description 1
- 101100269671 Dictyostelium discoideum alrA gene Proteins 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 101100215660 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) afoB gene Proteins 0.000 description 1
- 102100039327 Enoyl-[acyl-carrier-protein] reductase, mitochondrial Human genes 0.000 description 1
- 241001379910 Ephemera danica Species 0.000 description 1
- 101100350700 Escherichia coli (strain K12) paaB gene Proteins 0.000 description 1
- 101100350708 Escherichia coli (strain K12) paaF gene Proteins 0.000 description 1
- 101100350709 Escherichia coli (strain K12) paaG gene Proteins 0.000 description 1
- 101100350710 Escherichia coli (strain K12) paaH gene Proteins 0.000 description 1
- 101100082074 Escherichia coli (strain K12) paaZ gene Proteins 0.000 description 1
- 101100321116 Escherichia coli (strain K12) yqhD gene Proteins 0.000 description 1
- 241000660147 Escherichia coli str. K-12 substr. MG1655 Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 108010074122 Ferredoxins Proteins 0.000 description 1
- 108010080982 Formate-tetrahydrofolate ligase Proteins 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 241000606768 Haemophilus influenzae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 101000974934 Homo sapiens Cyclic AMP-dependent transcription factor ATF-2 Proteins 0.000 description 1
- 101000961707 Homo sapiens Enoyl-[acyl-carrier-protein] reductase, mitochondrial Proteins 0.000 description 1
- 101000997829 Homo sapiens Glial cell line-derived neurotrophic factor Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108010020056 Hydrogenase Proteins 0.000 description 1
- 102000005298 Iron-Sulfur Proteins Human genes 0.000 description 1
- 108010081409 Iron-Sulfur Proteins Proteins 0.000 description 1
- 101100236497 Klebsiella aerogenes maoC gene Proteins 0.000 description 1
- 241000588749 Klebsiella oxytoca Species 0.000 description 1
- 101100433987 Latilactobacillus sakei subsp. sakei (strain 23K) ackA1 gene Proteins 0.000 description 1
- 102100025357 Lipid-phosphate phosphatase Human genes 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108010010685 Methenyltetrahydrofolate cyclohydrolase Proteins 0.000 description 1
- 102000005954 Methylenetetrahydrofolate Reductase (NADPH2) Human genes 0.000 description 1
- 108010030837 Methylenetetrahydrofolate Reductase (NADPH2) Proteins 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 108050006262 Mitochondrial carrier proteins Proteins 0.000 description 1
- 101100235161 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) lerI gene Proteins 0.000 description 1
- 101100269700 Mycolicibacterium smegmatis alr gene Proteins 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 102000000818 NADP Transhydrogenases Human genes 0.000 description 1
- 108010001609 NADP Transhydrogenases Proteins 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101150050255 PDC1 gene Proteins 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 229920002984 Paramylon Polymers 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 102000013566 Plasminogen Human genes 0.000 description 1
- 108010051456 Plasminogen Proteins 0.000 description 1
- 102000001938 Plasminogen Activators Human genes 0.000 description 1
- 108010001014 Plasminogen Activators Proteins 0.000 description 1
- 102000055027 Protein Methyltransferases Human genes 0.000 description 1
- 108700040121 Protein Methyltransferases Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 101100350716 Pseudomonas putida paaK gene Proteins 0.000 description 1
- 241000205156 Pyrococcus furiosus Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 101100332697 Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) fadB1 gene Proteins 0.000 description 1
- 241001524101 Rhodococcus opacus Species 0.000 description 1
- 244000154511 Rosa hybrid cultivar Species 0.000 description 1
- 235000010295 Rosa x kordesii Nutrition 0.000 description 1
- 101100462087 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LAT1 gene Proteins 0.000 description 1
- 101100009692 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LPD1 gene Proteins 0.000 description 1
- 101100350214 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDA1 gene Proteins 0.000 description 1
- 101100027944 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDB1 gene Proteins 0.000 description 1
- 101100518193 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDX1 gene Proteins 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 102100024639 Short-chain specific acyl-CoA dehydrogenase, mitochondrial Human genes 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 241000534944 Thia Species 0.000 description 1
- 102000005488 Thioesterase Human genes 0.000 description 1
- 101710167005 Thiol:disulfide interchange protein DsbD Proteins 0.000 description 1
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 description 1
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 description 1
- 241000589892 Treponema denticola Species 0.000 description 1
- 101100257457 Variovorax paradoxus acd gene Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 241000222124 [Candida] boidinii Species 0.000 description 1
- 101150068224 acdA gene Proteins 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 101150006213 ackA gene Proteins 0.000 description 1
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000002730 additional effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 101150014383 adhE gene Proteins 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 230000009603 aerobic growth Effects 0.000 description 1
- 150000001336 alkenes Chemical group 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 101150083358 alrA gene Proteins 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940053200 antiepileptics fatty acid derivative Drugs 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003416 augmentation Effects 0.000 description 1
- 238000010364 biochemical engineering Methods 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 239000003225 biodiesel Substances 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 108010083912 bleomycin N-acetyltransferase Proteins 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 108010031234 carbon monoxide dehydrogenase Proteins 0.000 description 1
- 239000007833 carbon precursor Substances 0.000 description 1
- 239000003575 carbonaceous material Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 description 1
- 239000005516 coenzyme A Substances 0.000 description 1
- 235000017471 coenzyme Q10 Nutrition 0.000 description 1
- 229940093530 coenzyme a Drugs 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000007357 dehydrogenase reaction Methods 0.000 description 1
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940120503 dihydroxyacetone Drugs 0.000 description 1
- 238000004851 dishwashing Methods 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 150000002118 epoxides Chemical class 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 108010083294 ethanol acyltransferase Proteins 0.000 description 1
- 101150115959 fadR gene Proteins 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- SXMOKYXNAPLNCW-GORZOVPNSA-N formyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 SXMOKYXNAPLNCW-GORZOVPNSA-N 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 101150075213 frdA gene Proteins 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 230000009229 glucose formation Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- MWVFCEVNXHTDNF-UHFFFAOYSA-N hexane-2,3-dione Chemical group CCCC(=O)C(C)=O MWVFCEVNXHTDNF-UHFFFAOYSA-N 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000007037 hydroformylation reaction Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical class [H]O* 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical group [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000003317 industrial substance Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000010687 lubricating oil Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 239000010742 number 1 fuel oil Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 230000005789 organism growth Effects 0.000 description 1
- 101150037784 paaA gene Proteins 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 101150088738 pckA gene Proteins 0.000 description 1
- 101150067708 pckG gene Proteins 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 108010038136 phospho-2-keto-3-deoxy-gluconate aldolase Proteins 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 229940127126 plasminogen activator Drugs 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 101150023641 ppc gene Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- QAQREVBBADEHPA-IEXPHMLFSA-N propionyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QAQREVBBADEHPA-IEXPHMLFSA-N 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- VAAHKRMGOFIORX-SNIDVWGTSA-N s-[2-[3-[[(2r)-4-[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] 3-hydroxyhexanethioate Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)CCC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VAAHKRMGOFIORX-SNIDVWGTSA-N 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 229930195734 saturated hydrocarbon Natural products 0.000 description 1
- 150000003333 secondary alcohols Chemical class 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004152 substrate-level phosphorylation Effects 0.000 description 1
- 101150031436 sucD gene Proteins 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 238000006491 synthase reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 150000003509 tertiary alcohols Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004227 thermal cracking Methods 0.000 description 1
- 101150084216 thiB gene Proteins 0.000 description 1
- 108020002982 thioesterase Proteins 0.000 description 1
- 229960000187 tissue plasminogen activator Drugs 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 101150080369 tpiA gene Proteins 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 229940035936 ubiquinone Drugs 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000002916 wood waste Substances 0.000 description 1
- 239000007222 ypd medium Substances 0.000 description 1
- 101150049562 yqeF gene Proteins 0.000 description 1
- UZVNCLCLJHPHIF-NOJKMYKQSA-J zinc;(1e)-2-(ethylcarbamoylamino)-n-methoxy-2-oxoethanimidoyl cyanide;manganese(2+);n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[Zn+2].[S-]C(=S)NCCNC([S-])=S.[S-]C(=S)NCCNC([S-])=S.CCNC(=O)NC(=O)C(\C#N)=N\OC UZVNCLCLJHPHIF-NOJKMYKQSA-J 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01035—3-Hydroxyacyl-CoA dehydrogenase (1.1.1.35)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/0105—Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01016—Acetyl-CoA C-acyltransferase (2.3.1.16)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01017—Enoyl-CoA hydratase (4.2.1.17), i.e. crotonase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3414608P | 2008-03-05 | 2008-03-05 | |
| US9017108P | 2008-08-19 | 2008-08-19 | |
| US11050008P | 2008-10-31 | 2008-10-31 | |
| PCT/US2009/036242 WO2009111672A1 (en) | 2008-03-05 | 2009-03-05 | Primary alcohol producing organisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2262901T3 true DK2262901T3 (en) | 2019-01-21 |
Family
ID=41056374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09717987.3T DK2262901T3 (en) | 2008-03-05 | 2009-03-05 | ORGANISMS PRODUCING PRIMARY ALCOHOL |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US7977084B2 (enExample) |
| EP (2) | EP2262901B1 (enExample) |
| JP (2) | JP5755884B2 (enExample) |
| BR (1) | BRPI0909690A2 (enExample) |
| CA (2) | CA2717586C (enExample) |
| DK (1) | DK2262901T3 (enExample) |
| ES (1) | ES2703775T3 (enExample) |
| PL (1) | PL2262901T3 (enExample) |
| SI (1) | SI2262901T1 (enExample) |
| WO (1) | WO2009111672A1 (enExample) |
| ZA (1) | ZA201007065B (enExample) |
Families Citing this family (81)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8110670B2 (en) | 2006-05-19 | 2012-02-07 | Ls9, Inc. | Enhanced production of fatty acid derivatives |
| US20100242345A1 (en) | 2006-05-19 | 2010-09-30 | LS9, Inc | Production of fatty acids & derivatives thereof |
| US20100274033A1 (en) | 2009-04-27 | 2010-10-28 | Ls9, Inc. | Production of fatty acid esters |
| BRPI0811747A8 (pt) | 2007-05-17 | 2014-10-14 | Univ Illinois | Métodos e composições para a produção de solventes |
| CA2712779C (en) | 2008-01-22 | 2021-03-16 | Genomatica, Inc. | Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol |
| BRPI0909690A2 (pt) | 2008-03-05 | 2019-02-26 | Genomatica Inc | organismos produtores de alcoóis primários |
| CN105483068A (zh) | 2008-05-16 | 2016-04-13 | Reg生命科学有限责任公司 | 产生碳氢化合物的方法和组合物 |
| WO2009155382A1 (en) | 2008-06-17 | 2009-12-23 | Genomatica, Inc. | Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate |
| US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
| EP2352824B1 (en) | 2008-10-07 | 2015-09-02 | REG Life Sciences, LLC | Method and compositions for producing fatty aldehydes |
| BRPI0920010A2 (pt) | 2008-10-28 | 2016-04-19 | Ls9 Inc | métodos para produzir álcool graxo e surfactante |
| WO2010057022A1 (en) * | 2008-11-14 | 2010-05-20 | Genomatica, Inc. | Microorganisms for the production of methyl ethyl ketone and 2-butanol |
| WO2010077806A1 (en) * | 2008-12-15 | 2010-07-08 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways |
| JP2012511928A (ja) | 2008-12-16 | 2012-05-31 | ゲノマチカ， インク． | 合成ガス及び他の炭素源の有用な製品への変換のための微生物及び方法 |
| US20100279354A1 (en) * | 2009-04-29 | 2010-11-04 | Evolugate, Llc | Adapting microorganisms for agricultural products |
| WO2010144746A2 (en) * | 2009-06-10 | 2010-12-16 | Genomatica, Inc. | Microorganisms and methods for carbon-efficient biosynthesis of mek and 2-butanol |
| EP2277989A1 (en) | 2009-07-24 | 2011-01-26 | Technische Universiteit Delft | Fermentative glycerol-free ethanol production |
| KR20120120493A (ko) | 2009-12-10 | 2012-11-01 | 게노마티카 인코포레이티드 | 합성 가스 또는 기타 가스상 탄소원 및 메탄올을 1,3-부탄디올로 변환하는 방법 및 변환용 유기체 |
| US8445244B2 (en) | 2010-02-23 | 2013-05-21 | Genomatica, Inc. | Methods for increasing product yields |
| ES2741637T3 (es) | 2010-03-09 | 2020-02-11 | Mitsui Chemicals Inc | Bacteria productora de alcohol isopropílico altamente productora |
| WO2011127409A2 (en) | 2010-04-08 | 2011-10-13 | Ls9, Inc. | Methods and compositions related to fatty alcohol biosynthetic enzymes |
| US20110269201A1 (en) * | 2010-04-30 | 2011-11-03 | Qteros, Inc. | Redirected bioenergetics in recombinant cellulolytic clostridium microorganisms |
| CA2798452C (en) * | 2010-05-05 | 2019-09-03 | Mascoma Corporation | Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate |
| KR20130071433A (ko) | 2010-05-07 | 2013-06-28 | 그린라이트 바이오사이언시스, 아이엔씨. | 효소의 재배치를 통한 대사 경로에서의 흐름의 제어 방법 |
| WO2012009523A2 (en) | 2010-07-15 | 2012-01-19 | The Procter & Gamble Company | A personal care composition comprising a near-terminal branched compound |
| US10138504B2 (en) | 2010-08-06 | 2018-11-27 | Lallemand Hungary Liquidity Management Llc | Production of malonyl-CoA derived products via anaerobic pathways |
| DK3219796T3 (da) | 2010-08-31 | 2020-12-21 | Greenlight Biosciences Inc | Fremgangsmåder til kontrol af strømningen i metaboliske reaktionsveje ved hjælp af proteasemanipulation |
| EP2655612B1 (en) * | 2010-12-23 | 2017-07-19 | Shell Internationale Research Maatschappij B.V. | Gene disruptants producing fatty acyl-coa derivatives |
| EP2673369B1 (en) | 2011-02-07 | 2017-04-05 | William Marsh Rice University | Reverse beta oxidation pathway |
| EP2675784B1 (en) | 2011-02-17 | 2017-09-13 | The Procter and Gamble Company | Bio-based linear alkylphenyl sulfonates |
| CN103380204B (zh) | 2011-02-17 | 2016-02-03 | 宝洁公司 | 包含c10-c13烷基苯基磺酸盐的混合物的组合物 |
| US9594869B2 (en) | 2011-03-01 | 2017-03-14 | Rutgers, The State University Of New Jersey | Genetically engineered microbes and uses thereof |
| CN102174419B (zh) * | 2011-03-24 | 2013-04-03 | 南京工业大学 | 一株米根霉菌株及其诱变筛选方法和发酵生产富马酸的方法 |
| WO2012177726A1 (en) * | 2011-06-22 | 2012-12-27 | Genomatica, Inc. | Microorganism for producing primary alcohols and related compounds and methods related thereto |
| WO2013003432A1 (en) * | 2011-06-29 | 2013-01-03 | Genomatica, Inc. | Microorganisms for producing succinate and methods related thereto |
| CA2841175C (en) * | 2011-07-12 | 2016-02-02 | Scientist Of Fortune S.A. | Recombinant microorganism for the production of useful metabolites |
| CN103842502A (zh) | 2011-08-03 | 2014-06-04 | Ls9公司 | 制备具有改良的脂肪链长度和饱和度特性的脂肪酸及其衍生物 |
| CN103998600A (zh) * | 2011-09-07 | 2014-08-20 | 威廉马什莱斯大学 | 通过反向脂肪酸氧化的官能化羧酸和醇 |
| JP2014526250A (ja) | 2011-09-09 | 2014-10-06 | グリーンライト バイオサイエンシーズ インコーポレーテッド | カルバペネムの無細胞調製 |
| DE102012207921A1 (de) | 2012-05-11 | 2013-11-14 | Evonik Industries Ag | Mehrstufiges Syntheseverfahren mit Synthesegas |
| KR101464656B1 (ko) | 2012-06-15 | 2014-12-02 | 한국생명공학연구원 | 발효산물 고생성능을 가지는 변이 미생물 및 이를 이용한 발효산물의 제조방법 |
| WO2014004616A2 (en) * | 2012-06-26 | 2014-01-03 | Gevo, Inc. | Engineered yeast with improved growth under low aeration |
| WO2014016328A1 (en) * | 2012-07-25 | 2014-01-30 | Total Marketing Services | Genetically engineered micro-organisms for production of fatty acids |
| CA2881666A1 (en) | 2012-08-10 | 2014-02-13 | Opx Biotechnologies, Inc. | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| CA2888197C (en) * | 2012-10-15 | 2023-03-07 | Genomatica, Inc. | Microorganisms and methods for production of specific length fatty alcohols and related compounds |
| AU2013364025A1 (en) | 2012-12-21 | 2015-07-23 | Greenlight Biosciences, Inc. | Cell-free system for converting methane into fuel, pyruvate or isobutanol |
| BR112015020827A2 (pt) * | 2013-02-28 | 2017-10-10 | Euglena Co Ltd | método para a introdução de gene em euglena, e transformante do mesmo |
| JP2014193153A (ja) * | 2013-02-28 | 2014-10-09 | Euglena Co Ltd | ユーグレナへの遺伝子導入方法 |
| US9267158B2 (en) | 2013-03-14 | 2016-02-23 | Intrexon Corporation | Biological production of multi-carbon compounds from methane |
| WO2014146026A1 (en) | 2013-03-15 | 2014-09-18 | Opx Biotechnologies, Inc. | Bioproduction of chemicals |
| US20160040172A1 (en) | 2013-03-15 | 2016-02-11 | Genomatica, Inc. | Microorganisms and methods for producing butadiene and related compounds by formate assimilation |
| BR112016001026A2 (pt) * | 2013-07-19 | 2017-10-24 | Cargill Inc | organismo geneticamente modificado |
| US11408013B2 (en) | 2013-07-19 | 2022-08-09 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| US9688977B2 (en) | 2013-08-05 | 2017-06-27 | Greenlight Biosciences, Inc. | Engineered phosphoglucose isomerase proteins with a protease cleavage site |
| CN103571764B (zh) * | 2013-08-26 | 2015-06-10 | 天津科技大学 | 一种高产中链脂肪酸乙酯的酿酒酵母工程菌及其构建方法 |
| US10704050B2 (en) | 2013-10-18 | 2020-07-07 | Biopetrolia Ab | Engineering of acetyl-CoA metabolism in yeast |
| EP3077501B1 (en) | 2013-12-03 | 2021-07-28 | Genomatica, Inc. | Microorganisms and methods for improving product yields on methanol using acetyl-coa synthesis |
| CN113416686B (zh) | 2013-12-27 | 2024-08-06 | 基因组股份公司 | 具有增加的碳通量效率的方法和生物 |
| JP6189793B2 (ja) | 2014-05-30 | 2017-08-30 | トヨタ自動車株式会社 | 組換え微生物及び当該組換え微生物を用いた物質製造方法 |
| JP6034332B2 (ja) | 2014-05-30 | 2016-11-30 | トヨタ自動車株式会社 | 組換え微生物及び当該組換え微生物を用いた物質製造方法 |
| DK3155424T3 (en) | 2014-06-12 | 2018-04-23 | Hyglos Invest Gmbh | Disassembly of endotoxins in solution |
| EP3470840B1 (en) | 2014-06-12 | 2021-03-03 | Hyglos Invest GmbH | Unmasking endotoxins in solution |
| EP2993228B1 (en) | 2014-09-02 | 2019-10-09 | Cargill, Incorporated | Production of fatty acid esters |
| US20170298363A1 (en) | 2014-09-18 | 2017-10-19 | Genomatica, Inc | Non-natural microbial organisms with improved energetic efficiency |
| AU2016243623B2 (en) | 2015-03-30 | 2022-04-14 | Greenlight Biosciences, Inc. | Cell-free production of ribonucleic acid |
| US11319562B2 (en) | 2015-04-15 | 2022-05-03 | Ramon Gonzalez | Modified fatty acid biosynthesis with ACP-dependent thiolases |
| WO2016168681A1 (en) * | 2015-04-15 | 2016-10-20 | William Marsh Rice University | Iterative platform for the synthesis of alpha functionalized products |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| CN105331647B (zh) * | 2015-07-03 | 2019-02-22 | 赣南师范大学 | 一种微生物体内合成脂肪醇乙酸酯的方法 |
| US10301653B2 (en) * | 2015-07-06 | 2019-05-28 | Wisconsin Alumni Research Foundation | Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use |
| US11441128B2 (en) | 2015-10-30 | 2022-09-13 | Genomatica, Inc. | Methanol dehydrogenase fusion proteins |
| EP3440215A1 (en) | 2016-04-06 | 2019-02-13 | Greenlight Biosciences, Inc. | Cell-free production of ribonucleic acid |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| CA3049386A1 (en) | 2017-01-06 | 2018-07-12 | Greenlight Biosciences, Inc. | Cell-free production of sugars |
| US11345938B2 (en) | 2017-02-02 | 2022-05-31 | Cargill, Incorporated | Genetically modified cells that produce C6-C10 fatty acid derivatives |
| AR113764A1 (es) | 2017-10-11 | 2020-06-10 | Greenlight Biosciences Inc | Métodos y composiciones para la producción de nucleósido trifosfato y ácido ribonucleico |
| US20210079334A1 (en) | 2018-01-30 | 2021-03-18 | Genomatica, Inc. | Fermentation systems and methods with substantially uniform volumetric uptake rate of a reactive gaseous component |
| US11541105B2 (en) | 2018-06-01 | 2023-01-03 | The Research Foundation For The State University Of New York | Compositions and methods for disrupting biofilm formation and maintenance |
| JP2021528977A (ja) | 2018-06-26 | 2021-10-28 | ジェノマティカ， インコーポレイテッド | グリセルアルデヒド３−リン酸（ｇ３ｐ）を３−ホスホグリセリン酸（３ｐｇ）に変換する酵素および／またはフルクトース−１，６−ビスホスファターゼを有する組換え微生物（合成または増強されたメチロトロフィーを有するものを含む） |
| WO2023178261A1 (en) * | 2022-03-16 | 2023-09-21 | Genomatica, Inc. | Microbial production of z3-hexenol, z3-hexenal and z3-hexenyl acetate |
Family Cites Families (180)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3513209A (en) * | 1968-08-19 | 1970-05-19 | Du Pont | Method of making 1,4-cyclohexadiene |
| US4076948A (en) * | 1968-10-10 | 1978-02-28 | El Paso Products Company | Process for treatment of adipic acid mother liquor |
| GB1230276A (enExample) | 1968-12-09 | 1971-04-28 | ||
| US3965182A (en) * | 1969-10-02 | 1976-06-22 | Ethyl Corporation | Preparation of aniline from phenol and ammonia |
| JPS506776Y1 (enExample) | 1969-12-27 | 1975-02-26 | ||
| JPS4831084B1 (enExample) | 1970-09-04 | 1973-09-26 | ||
| GB1344557A (en) | 1972-06-23 | 1974-01-23 | Mitsubishi Petrochemical Co | Process for preparing 1,4-butanediol |
| JPS506776A (enExample) | 1973-05-28 | 1975-01-23 | ||
| DE2455617C3 (de) * | 1974-11-23 | 1982-03-18 | Basf Ag, 6700 Ludwigshafen | Verfahren zur Herstellung von Butandiol und/oder Tetrahydrofuran über die Zwischenstufe des γ-Butyrolactons |
| DE2501499A1 (de) | 1975-01-16 | 1976-07-22 | Hoechst Ag | Verfahren zur herstellung von butandiol-(1.4) |
| US4344002A (en) | 1978-02-22 | 1982-08-10 | Supertex, Inc. | Detection circuit and structure therefor |
| US4301077A (en) * | 1980-12-22 | 1981-11-17 | Standard Oil Company | Process for the manufacture of 1-4-butanediol and tetrahydrofuran |
| IT1190783B (it) | 1981-04-29 | 1988-02-24 | Davy Mckee Oil & Chem | Processo per l'idrogenolisi di esteri di acidi carbossilici |
| US4652685A (en) * | 1985-11-15 | 1987-03-24 | General Electric Company | Hydrogenation of lactones to glycols |
| EP0249773B1 (en) * | 1986-06-11 | 1992-12-16 | Michigan Biotechnology Institute | A process for the production of succinic acid by anaerobic fermentation |
| US5143834A (en) * | 1986-06-11 | 1992-09-01 | Glassner David A | Process for the production and purification of succinic acid |
| US5168055A (en) * | 1986-06-11 | 1992-12-01 | Rathin Datta | Fermentation and purification process for succinic acid |
| US5182199A (en) * | 1987-05-27 | 1993-01-26 | Hartley Brian S | Thermophilic ethanol production in a two-stage closed system |
| EP0633319B1 (en) * | 1988-04-27 | 1999-03-17 | Daicel Chemical Industries, Ltd. | Process for producing optically active 1,3-butanediol |
| WO1990012888A1 (en) * | 1989-04-27 | 1990-11-01 | Biocontrol Systems, Incorporated | Precipitate test for microorganisms |
| US5192673A (en) * | 1990-04-30 | 1993-03-09 | Michigan Biotechnology Institute | Mutant strain of C. acetobutylicum and process for making butanol |
| US5079143A (en) * | 1990-05-02 | 1992-01-07 | The Upjohn Company | Method of indentifying compounds useful as antiparasitic drugs |
| DE69132472T2 (de) | 1990-10-15 | 2001-03-15 | Daicel Chemical Industries, Ltd. | Verfahren zur Herstellung von optisch aktivem 1,3-Butandiol |
| US5173429A (en) * | 1990-11-09 | 1992-12-22 | The Board Of Trustees Of The University Of Arkansas | Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism |
| IL100572A (en) | 1991-01-03 | 1997-01-10 | Lepetit Spa | Amides of antibiotic ge 2270 factors their preparation and pharmaceutical compositions containing them |
| US5416020A (en) * | 1992-09-29 | 1995-05-16 | Bio-Technical Resources | Lactobacillus delbrueckii ssp. bulgaricus strain and fermentation process for producing L-(+)-lactic acid |
| US5807722A (en) * | 1992-10-30 | 1998-09-15 | Bioengineering Resources, Inc. | Biological production of acetic acid from waste gases with Clostridium ljungdahlii |
| US6136577A (en) * | 1992-10-30 | 2000-10-24 | Bioengineering Resources, Inc. | Biological production of ethanol from waste gases with Clostridium ljungdahlii |
| FR2702492B1 (fr) * | 1993-03-12 | 1995-05-24 | Rhone Poulenc Chimie | Procédé de production par fermentation d'acide itaconique. |
| US5487987A (en) * | 1993-09-16 | 1996-01-30 | Purdue Research Foundation | Synthesis of adipic acid from biomass-derived carbon sources |
| US5521075A (en) | 1994-12-19 | 1996-05-28 | Michigan Biotechnology Institute | Method for making succinic acid, anaerobiospirillum succiniciproducens variants for use in process and methods for obtaining variants |
| US5504004A (en) * | 1994-12-20 | 1996-04-02 | Michigan Biotechnology Institute | Process for making succinic acid, microorganisms for use in the process and methods of obtaining the microorganisms |
| US5700934A (en) * | 1995-03-01 | 1997-12-23 | Dsm N.V. | Process for the preparation of epsilon-caprolactam and epsilon-caprolactam precursors |
| US5478952A (en) * | 1995-03-03 | 1995-12-26 | E. I. Du Pont De Nemours And Company | Ru,Re/carbon catalyst for hydrogenation in aqueous solution |
| US5863782A (en) | 1995-04-19 | 1999-01-26 | Women's And Children's Hospital | Synthetic mammalian sulphamidase and genetic sequences encoding same |
| US5686276A (en) * | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| FR2736927B1 (fr) * | 1995-07-18 | 1997-10-17 | Rhone Poulenc Fibres & Polymer | Enzymes a activite amidase, outils genetiques et microorganismes hotes permettant leur obtention et procede d'hydrolyse mettant en oeuvre lesdites enzymes |
| US5573931A (en) * | 1995-08-28 | 1996-11-12 | Michigan Biotechnology Institute | Method for making succinic acid, bacterial variants for use in the process, and methods for obtaining variants |
| US5770435A (en) * | 1995-11-02 | 1998-06-23 | University Of Chicago | Mutant E. coli strain with increased succinic acid production |
| US5869301A (en) * | 1995-11-02 | 1999-02-09 | Lockhead Martin Energy Research Corporation | Method for the production of dicarboxylic acids |
| JP2000506012A (ja) * | 1996-02-27 | 2000-05-23 | ミシガン ステイト ユニヴァーシティー | サーモアンエアロバクター・エタノリカス３９ｅの第二級アルコールデヒドロゲナーゼをコードする遺伝子のクローニング及び発現並びに酵素の生化学的特徴付け |
| US5958745A (en) * | 1996-03-13 | 1999-09-28 | Monsanto Company | Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants |
| JP4101295B2 (ja) | 1996-07-01 | 2008-06-18 | バイオエンジニアリング・リソーシズ・インコーポレーテツド | 廃ガスからの酢酸の生物学的生産 |
| KR100459818B1 (ko) * | 1996-09-02 | 2004-12-03 | 이. 아이. 두퐁 드 느무르 앤드 컴퍼니 | ε-카프로락탐의 제조방법 |
| US6117658A (en) * | 1997-02-13 | 2000-09-12 | James Madison University | Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units |
| US6274790B1 (en) * | 1997-04-14 | 2001-08-14 | The University Of British Columbia | Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis |
| KR19990013007A (ko) * | 1997-07-31 | 1999-02-25 | 박원훈 | 형질전환된 대장균 ｓｓ373(ｋｃｔｃ 8818ｐ)과 이를 이용한숙신산의 생산방법 |
| JPH11103863A (ja) * | 1997-10-08 | 1999-04-20 | Nippon Shokubai Co Ltd | マレイン酸異性化酵素遺伝子 |
| US6280986B1 (en) * | 1997-12-01 | 2001-08-28 | The United States Of America As Represented By The Secretary Of Agriculture | Stabilization of pet operon plasmids and ethanol production in bacterial strains lacking lactate dehydrogenase and pyruvate formate lyase activities |
| US20030087381A1 (en) * | 1998-04-13 | 2003-05-08 | University Of Georgia Research Foundation, Inc. | Metabolically engineered organisms for enhanced production of oxaloacetate-derived biochemicals |
| DK1073722T3 (da) * | 1998-04-13 | 2010-06-07 | Univ Georgia | Pyruvat-carboxylase-overekspression til øget produktion af biokemikalier, der er afledt af oxaloacetat, i mikrobielle celler |
| US6159738A (en) * | 1998-04-28 | 2000-12-12 | University Of Chicago | Method for construction of bacterial strains with increased succinic acid production |
| DE19820652A1 (de) | 1998-05-08 | 1999-11-11 | Basf Ag | Kationische Rutheniumkomplexe, Verfahren zu ihrer Herstellung und ihre Verwendung |
| US6432686B1 (en) | 1998-05-12 | 2002-08-13 | E. I. Du Pont De Nemours And Company | Method for the production of 1,3-propanediol by recombinant organisms comprising genes for vitamin B12 transport |
| US6444784B1 (en) * | 1998-05-29 | 2002-09-03 | Exxonmobil Research & Engineering Company | Wax crystal modifiers (LAW657) |
| US6187569B1 (en) | 1998-07-02 | 2001-02-13 | E. I. Du Pont De Nemours And Company | Microbial production of terephthalic acid and isophthalic acid |
| DE19856136C2 (de) | 1998-12-04 | 2002-10-24 | Pasteur Institut | Verfahren und Vorrichtung zur Selektion beschleunigter Proliferation lebender Zellen in Suspension |
| AU3482200A (en) | 1999-02-02 | 2000-08-25 | Bernhard Palsson | Methods for identifying drug targets based on genomic sequence data |
| US6686310B1 (en) * | 1999-02-09 | 2004-02-03 | E. I. Du Pont De Nemours And Company | High surface area sol-gel route prepared hydrogenation catalysts |
| US6365376B1 (en) * | 1999-02-19 | 2002-04-02 | E. I. Du Pont De Nemours And Company | Genes and enzymes for the production of adipic acid intermediates |
| EP1159435A1 (en) * | 1999-03-05 | 2001-12-05 | Monsanto Technology LLC | Multigene expression vectors for the biosynthesis of products via multienzyme biological pathways |
| US6852517B1 (en) * | 1999-08-30 | 2005-02-08 | Wisconsin Alumni Research Foundation | Production of 3-hydroxypropionic acid in recombinant organisms |
| EP1124979B1 (en) | 1999-08-30 | 2006-08-30 | Wisconsin Alumni Research Foundation | Production of 3-hydroxypropionic acid in recombinant organisms |
| US6660857B2 (en) * | 2000-02-03 | 2003-12-09 | Dsm N.V. | Process for the preparation of ε-caprolactam |
| US6878861B2 (en) | 2000-07-21 | 2005-04-12 | Washington State University Research Foundation | Acyl coenzyme A thioesterases |
| PL205622B1 (pl) * | 2000-07-25 | 2010-05-31 | Emmaus Foundation | Ciągły sposób wytwarzania etanolu w beztlenowej fermentacji bakteryjnej |
| US7233567B1 (en) * | 2000-09-22 | 2007-06-19 | Nortel Networks Limited | Apparatus and method for supporting multiple traffic redundancy mechanisms |
| AU2002219818B2 (en) * | 2000-11-20 | 2007-08-16 | Cargill, Incorporated | 3-hydroxypropionic acid and other organic compounds |
| CA2430270A1 (en) * | 2000-11-22 | 2002-05-30 | Cargill Dow Polymers, Llc | Methods and materials for the synthesis of organic products |
| CN1358841A (zh) | 2000-12-11 | 2002-07-17 | 云南省微生物研究所 | 云南链霉菌 |
| WO2002053746A1 (fr) * | 2000-12-28 | 2002-07-11 | Toyota Jidosha Kabushiki Kaisha | Procédé de production d'alcool prényle |
| JP4776146B2 (ja) | 2001-01-10 | 2011-09-21 | ザ・ペン・ステート・リサーチ・ファンデーション | 細胞代謝をモデル化する方法及びシステム |
| US7127379B2 (en) | 2001-01-31 | 2006-10-24 | The Regents Of The University Of California | Method for the evolutionary design of biochemical reaction networks |
| EP1381860A4 (en) * | 2001-03-01 | 2008-10-15 | Univ California | MODELS AND METHOD FOR DETERMINING SYSTEMIC PROPERTIES OF REGULATED RESPONSE NETWORKS |
| US6743610B2 (en) * | 2001-03-30 | 2004-06-01 | The University Of Chicago | Method to produce succinic acid from raw hydrolysates |
| MXPA03010183A (es) | 2001-05-07 | 2004-03-16 | Cargill Inc | Proceso para preparar acidos carboxilicos y derivados de los mismos. |
| JP4630486B2 (ja) | 2001-05-28 | 2011-02-09 | ダイセル化学工業株式会社 | 新規な（ｒ）−２，３−ブタンジオール脱水素酵素、その製造方法、及びこれを利用した光学活性アルコールの製造方法 |
| EP1409702A1 (en) | 2001-07-20 | 2004-04-21 | Youming Zhang | Improved rect or recet cloning and subcloning method |
| CA2356540A1 (en) * | 2001-08-30 | 2003-02-28 | Emory University | Expressed dna sequences involved in mitochondrial functions |
| US7256016B2 (en) * | 2001-11-02 | 2007-08-14 | Rice University | Recycling system for manipulation of intracellular NADH availability |
| ATE432338T1 (de) * | 2002-01-18 | 2009-06-15 | Novozymes As | Alanin-2,3-aminomutase |
| WO2003066863A1 (fr) | 2002-02-06 | 2003-08-14 | Showa Denko K.K. | Gene reductase d'un compose carbonyle alpha-substitue-alpha, beta-insature |
| EP1485471B1 (en) | 2002-03-18 | 2007-08-29 | BioCatalytics Inc. | Alcohol dehydrogenases with high solvent and temperature stability |
| US20030224363A1 (en) | 2002-03-19 | 2003-12-04 | Park Sung M. | Compositions and methods for modeling bacillus subtilis metabolism |
| CA2480216A1 (en) | 2002-03-29 | 2003-10-09 | Genomatica, Inc. | Human metabolic models and methods |
| JPWO2003095651A1 (ja) * | 2002-05-10 | 2005-09-15 | 協和醗酵工業株式会社 | メバロン酸の製造方法 |
| CN100471950C (zh) | 2002-06-14 | 2009-03-25 | Dsmip财产有限公司 | 具有α-H-α-氨基酸酰胺消旋酶活性的多肽和编码该多肽的核酸 |
| US7856317B2 (en) * | 2002-06-14 | 2010-12-21 | Genomatica, Inc. | Systems and methods for constructing genomic-based phenotypic models |
| US7826975B2 (en) | 2002-07-10 | 2010-11-02 | The Penn State Research Foundation | Method for redesign of microbial production systems |
| EP1532516A4 (en) * | 2002-07-10 | 2007-05-23 | Penn State Res Found | Method for determining gene knockout strategies |
| US7413878B2 (en) * | 2002-07-15 | 2008-08-19 | Kosan Biosciences, Inc. | Recombinant host cells expressing atoAD and capable of making a polyketide using a starter unit |
| US7371558B2 (en) * | 2002-10-04 | 2008-05-13 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
| JP5064656B2 (ja) | 2002-10-15 | 2012-10-31 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 実施反応経路を同定するための方法およびシステム |
| US20040152159A1 (en) * | 2002-11-06 | 2004-08-05 | Causey Thomas B. | Materials and methods for the efficient production of acetate and other products |
| BRPI0406756A (pt) | 2003-01-13 | 2005-12-20 | Cargill Inc | Método para fabricação de agentes quìmicos industriais |
| JP4275666B2 (ja) * | 2003-02-24 | 2009-06-10 | 財団法人地球環境産業技術研究機構 | 微生物による高効率水素製造方法 |
| RU2268300C2 (ru) | 2003-04-07 | 2006-01-20 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ, ОБЛАДАЮЩИХ ПОВЫШЕННОЙ ЭКСПРЕССИЕЙ ГЕНА pckA |
| DK1647594T3 (da) | 2003-07-29 | 2010-05-25 | Res Inst Innovative Tech Earth | Koryneform bakteriel transformant og fremgangsmåde til fremstilling af dicarboxylsyre under anvendelse af denne |
| US7927859B2 (en) * | 2003-08-22 | 2011-04-19 | Rice University | High molar succinate yield bacteria by increasing the intracellular NADH availability |
| WO2005026349A1 (ja) * | 2003-09-17 | 2005-03-24 | Mitsubishi Chemical Corporation | 非アミノ有機酸の製造方法 |
| EP1664286A1 (en) | 2003-09-18 | 2006-06-07 | Ciba SC Holding AG | Alcohol dehydrogenases with increased solvent and temperature stability |
| FR2862068B1 (fr) | 2003-11-06 | 2007-10-12 | Metabolic Explorer Sa | Souches de microorganismes optimisees pour des voies de biosyntheses consommatrices de nadph |
| US7244610B2 (en) * | 2003-11-14 | 2007-07-17 | Rice University | Aerobic succinate production in bacteria |
| FR2864967B1 (fr) * | 2004-01-12 | 2006-05-19 | Metabolic Explorer Sa | Microorganisme evolue pour la production de 1,2-propanediol |
| US7491520B2 (en) | 2004-01-19 | 2009-02-17 | Dsm Ip Assets B.V. | Biochemical synthesis of 6-amino caproic acid |
| US7608700B2 (en) | 2004-03-08 | 2009-10-27 | North Carolina State University | Lactobacillus acidophilus nucleic acid sequences encoding stress-related proteins and uses therefor |
| DE102004029112B4 (de) | 2004-06-11 | 2007-06-21 | Julich Chiral Solutions Gmbh | Alkoholdehydrogenase zur stereoselektiven Gewinnung von Hydroxyverbindungen |
| DE102004031177A1 (de) | 2004-06-29 | 2006-01-19 | Henkel Kgaa | Neue Geruchsstoffe bildende Genprodukte von Bacillus licheniformis und darauf aufbauende verbesserte biotechnologische Produktionsverfahren |
| US7262046B2 (en) * | 2004-08-09 | 2007-08-28 | Rice University | Aerobic succinate production in bacteria |
| CN101044245B (zh) | 2004-08-27 | 2012-05-02 | 莱斯大学 | 具有增加的琥珀酸产量的突变大肠杆菌菌株 |
| JP4712716B2 (ja) * | 2004-09-09 | 2011-06-29 | 財団法人地球環境産業技術研究機構 | プロモーター機能を有するｄｎａ断片 |
| KR20070065870A (ko) | 2004-09-17 | 2007-06-25 | 라이스 유니버시티 | 다량의 숙신산염을 생산하는 세균 |
| US20070022497A1 (en) * | 2004-10-08 | 2007-01-25 | Basf Plant Science Gmbh | Trans-2-enoyl-coa reductase gene of euglena gracilis |
| US7569380B2 (en) * | 2004-12-22 | 2009-08-04 | Rice University | Simultaneous anaerobic production of isoamyl acetate and succinic acid |
| JP2006204255A (ja) | 2005-01-31 | 2006-08-10 | Canon Inc | アセチル−ＣｏＡアシルトランスフェラーゼ遺伝子破壊ポリヒドロキシアルカノエート生産菌、またこれを利用したポリヒドロキシアルカノエート生産方法 |
| WO2006109312A2 (en) | 2005-04-15 | 2006-10-19 | Vascular Biogenics Ltd. | Compositions containing beta 2-glycoprotein i-derived peptides for the prevention and/or treatment of vascular disease |
| WO2007001982A1 (en) | 2005-06-20 | 2007-01-04 | Archer-Daniels-Midland Company | Altered glyoxylate shunt for improved production of aspartate-derived amino acids and chemicals |
| KR100679638B1 (ko) * | 2005-08-19 | 2007-02-06 | 한국과학기술원 | 포메이트 디하이드로게나제 ｄ 또는 ｅ를 코딩하는 유전자로 형질전환된 미생물 및 이를 이용한 숙신산의 제조방법 |
| KR100676160B1 (ko) * | 2005-08-19 | 2007-02-01 | 한국과학기술원 | 말릭효소를 코딩하는 유전자로 형질전환된 재조합 미생물 및 이를 이용한 숙신산의 제조방법 |
| AU2006287257A1 (en) * | 2005-09-09 | 2007-03-15 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of succinate |
| US7901915B2 (en) | 2005-09-19 | 2011-03-08 | Basf Se | Biocatalytic manufacturing of (meth) acrylic esters |
| US9297028B2 (en) * | 2005-09-29 | 2016-03-29 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| NZ566406A (en) | 2005-10-26 | 2012-04-27 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| DE102006017760A1 (de) | 2006-03-24 | 2007-09-27 | Ufz-Umweltforschungszentrum Leipzig-Halle Gmbh | Verfahren zur enzymatischen Herstellung von 2-Hydroxy-2-methylcarbonsäuren |
| CA2650505A1 (en) | 2006-05-01 | 2008-02-14 | University Of Florida Research Foundation, Inc. | Ethanol production in non-recombinant hosts |
| US8206970B2 (en) * | 2006-05-02 | 2012-06-26 | Butamax(Tm) Advanced Biofuels Llc | Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase |
| US20100274033A1 (en) | 2009-04-27 | 2010-10-28 | Ls9, Inc. | Production of fatty acid esters |
| MX344588B (es) * | 2006-05-19 | 2016-12-20 | Reg Life Sciences Llc | Produccion de acidos grasos y derivados de los mismos. |
| US8110670B2 (en) | 2006-05-19 | 2012-02-07 | Ls9, Inc. | Enhanced production of fatty acid derivatives |
| US20100242345A1 (en) | 2006-05-19 | 2010-09-30 | LS9, Inc | Production of fatty acids & derivatives thereof |
| DE102006025821A1 (de) | 2006-06-02 | 2007-12-06 | Degussa Gmbh | Ein Enzym zur Herstellung von Mehylmalonatsemialdehyd oder Malonatsemialdehyd |
| US20080293101A1 (en) * | 2006-07-27 | 2008-11-27 | Peters Matthew W | Engineered microorganisms for increasing product yield in biotransformations, related methods and systems |
| RU2311231C1 (ru) | 2006-08-15 | 2007-11-27 | ООО "Объединенный центр исследований и разработок" | Катализатор для получения эфиров акриловой кислоты по реакции метатезиса диалкилмалеатов (варианты) и каталитическая композиция на его основе |
| BRPI0716212A2 (pt) | 2006-08-30 | 2013-10-15 | Cargill Inc | Beta-alanina/alfa-cetoglutarato aminotransferase para produção de ácido 3-hidroxipropiônico |
| EP2097528A2 (en) * | 2006-10-31 | 2009-09-09 | DSMIP Assets B.V. | Butanol production in a eukaryotic cell |
| US20090155869A1 (en) * | 2006-12-01 | 2009-06-18 | Gevo, Inc. | Engineered microorganisms for producing n-butanol and related methods |
| US8017364B2 (en) | 2006-12-12 | 2011-09-13 | Butamax(Tm) Advanced Biofuels Llc | Solvent tolerant microorganisms |
| WO2008080124A2 (en) * | 2006-12-21 | 2008-07-03 | Gevo, Inc. | Butanol production by metabolically engineered yeast |
| EP2126042A4 (en) | 2007-01-12 | 2010-08-04 | Univ Colorado Regents | COMPOSITIONS AND METHODS FOR INCREASING TOLERANCE FOR THE PRODUCTION BY MICRO-ORGANISMS OF PRODUCED ORGANIC CHEMICALS |
| EP2118266A4 (en) * | 2007-02-09 | 2010-05-19 | Univ California | BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS |
| US8110093B2 (en) | 2007-03-14 | 2012-02-07 | Ls9, Inc. | Process for producing low molecular weight hydrocarbons from renewable resources |
| TWI568847B (zh) * | 2007-03-16 | 2017-02-01 | 奇諾麥提卡公司 | 用於１，４-丁二醇及其前驅物之生物合成的組合物及方法 |
| AU2008230735A1 (en) | 2007-03-28 | 2008-10-02 | Ls9, Inc. | Enhanced production of fatty acid derivatives |
| US20100159546A1 (en) * | 2007-03-30 | 2010-06-24 | Aristos Aristidou | Metabolic engineering of yeasts for the production of 1-butanol |
| EP2147111A4 (en) * | 2007-04-18 | 2010-06-23 | Gevo Inc | MANIPULATED MICROORGANISMS FOR THE MANUFACTURE OF ISOPROPANOL |
| WO2008130995A2 (en) | 2007-04-18 | 2008-10-30 | E. I. Du Pont De Nemours And Company | Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes |
| US20080293060A1 (en) | 2007-04-23 | 2008-11-27 | Ls9, Inc. | Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes |
| US8426174B2 (en) | 2007-05-02 | 2013-04-23 | Butamax(Tm) Advanced Biofuels Llc | Method for the production of 2-butanol |
| US20080274522A1 (en) * | 2007-05-02 | 2008-11-06 | Bramucci Michael G | Method for the production of 2-butanone |
| BRPI0811747A8 (pt) | 2007-05-17 | 2014-10-14 | Univ Illinois | Métodos e composições para a produção de solventes |
| BRPI0813359A2 (pt) | 2007-05-22 | 2014-10-14 | Ls9 Inc | Ácidos nucleicos isolados, vetor, células, métodos para produzir um hidrocarboneto, para produzir uma cetona alifática, para produzir um polipeptídeo purificado, para produzir um hidrocarboneto, para produzir biocombustíveis, hidrocarboneto, biocombustível, polipeptídeo isolado, ácido nucleico isolado, organismos geneticamente engenheirados, método para identificar uma enzima útil para a produção de hidrocarbonetos |
| BRPI0811967B1 (pt) | 2007-06-01 | 2023-01-17 | Terravia Holdings, Inc | Microalga chlorella ou prototheca e método para produção de lipídios microbianos |
| BRPI0812515A2 (pt) | 2007-06-15 | 2014-12-30 | Evonik Degussa Gmbh | Micro-organismo com sistema de vitamina b12 desregulado |
| US20100199548A1 (en) | 2007-07-06 | 2010-08-12 | Ls9, Inc. | Systems and methods for the production of fatty esters |
| EP2017344A1 (en) | 2007-07-20 | 2009-01-21 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Production of itaconic acid |
| US20100205857A1 (en) | 2007-07-23 | 2010-08-19 | Einte-Karst Dijk | Butanol production in a eukaryotic cell |
| WO2009013158A1 (en) * | 2007-07-23 | 2009-01-29 | Dsm Ip Assets B.V. | Butanol production in a eukaryotic cell |
| EP2173881B1 (en) * | 2007-07-23 | 2016-09-28 | DSM IP Assets B.V. | Acetyl-coa producing enzymes in yeast |
| US8285554B2 (en) * | 2007-07-27 | 2012-10-09 | Dsp Group Limited | Method and system for dynamic aliasing suppression |
| US7947483B2 (en) * | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| KR101042242B1 (ko) | 2007-09-07 | 2011-06-17 | 한국과학기술원 | 1,4-부탄디올 생성능을 가지는 변이체 및 이를 이용한1,4-부탄디올의 제조방법 |
| WO2009049274A2 (en) | 2007-10-12 | 2009-04-16 | The Regents Of The University Of California | Microorganism engineered to produce isopropanol |
| US8183028B2 (en) | 2007-12-21 | 2012-05-22 | Ls9, Inc. | Methods and compositions for producing olefins |
| CA2712779C (en) | 2008-01-22 | 2021-03-16 | Genomatica, Inc. | Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol |
| WO2009103026A1 (en) | 2008-02-15 | 2009-08-20 | Gevo, Inc. | Engineered microorganisms for producing isopropanol |
| US20090246842A1 (en) | 2008-02-15 | 2009-10-01 | Gevo, Inc. | Engineered microorganisms for producing propanol |
| WO2009111513A1 (en) | 2008-03-03 | 2009-09-11 | Joule Biotechnologies, Inc. | Engineered co2 fixing microorganisms producing carbon-based products of interest |
| BRPI0909690A2 (pt) | 2008-03-05 | 2019-02-26 | Genomatica Inc | organismos produtores de alcoóis primários |
| HUE037878T2 (hu) | 2008-03-11 | 2018-09-28 | Genomatica Inc | Adipátészter vagy tioészter szintézis |
| CN109055450A (zh) | 2008-03-11 | 2018-12-21 | 基因组股份公司 | 从5-甲酰缬草酸制备6-氨基己酸 |
| PL2265709T3 (pl) | 2008-03-27 | 2018-05-30 | Genomatica, Inc. | Mikroorganizmy do produkcji kwasu adypinowego i innych związków |
| CN102016006B (zh) | 2008-04-25 | 2013-04-10 | 财团法人地球环境产业技术研究机构 | 具有异丙醇生产能力的棒状细菌转化体 |
| CN105483068A (zh) | 2008-05-16 | 2016-04-13 | Reg生命科学有限责任公司 | 产生碳氢化合物的方法和组合物 |
| WO2009155382A1 (en) * | 2008-06-17 | 2009-12-23 | Genomatica, Inc. | Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate |
| WO2010022090A1 (en) | 2008-08-18 | 2010-02-25 | Ls9, Inc. | Systems and methods for the production of mixed fatty esters |
| WO2010022763A1 (en) | 2008-08-25 | 2010-03-04 | Metabolic Explorer | Method for the preparation of 2-hydroxy-isobutyrate |
| US8354563B2 (en) * | 2008-10-16 | 2013-01-15 | Maverick Biofuels, Inc. | Methods and apparatus for synthesis of alcohols from syngas |
| BRPI0920010A2 (pt) * | 2008-10-28 | 2016-04-19 | Ls9 Inc | métodos para produzir álcool graxo e surfactante |
| ES2639413T3 (es) | 2008-12-12 | 2017-10-26 | Cj Research Center Llc | Procedimiento y composiciones ecológicas para la producción de productos químicos de poli(5HV) y de 5 carbonos |
| US9101598B2 (en) | 2011-11-30 | 2015-08-11 | Merial, Inc. | Recombinant Gallid herpesvirus 3 (MDV serotype 2) vectors expressing antigens of avian pathogens and uses thereof |
-
2009
- 2009-03-05 BR BRPI0909690A patent/BRPI0909690A2/pt not_active Application Discontinuation
- 2009-03-05 DK DK09717987.3T patent/DK2262901T3/en active
- 2009-03-05 WO PCT/US2009/036242 patent/WO2009111672A1/en not_active Ceased
- 2009-03-05 EP EP09717987.3A patent/EP2262901B1/en active Active
- 2009-03-05 SI SI200931911T patent/SI2262901T1/sl unknown
- 2009-03-05 EP EP18201495.1A patent/EP3450550A1/en active Pending
- 2009-03-05 CA CA2717586A patent/CA2717586C/en active Active
- 2009-03-05 CA CA3081506A patent/CA3081506A1/en active Pending
- 2009-03-05 PL PL09717987T patent/PL2262901T3/pl unknown
- 2009-03-05 US US12/398,996 patent/US7977084B2/en active Active
- 2009-03-05 JP JP2010549897A patent/JP5755884B2/ja not_active Expired - Fee Related
- 2009-03-05 ES ES09717987T patent/ES2703775T3/es active Active
-
2010
- 2010-10-04 ZA ZA2010/07065A patent/ZA201007065B/en unknown
-
2011
- 2011-06-24 US US13/168,833 patent/US9260729B2/en active Active
-
2014
- 2014-03-05 JP JP2014042892A patent/JP2014100147A/ja not_active Withdrawn
-
2016
- 2016-01-04 US US14/987,489 patent/US10208320B2/en active Active
-
2018
- 2018-12-26 US US16/233,018 patent/US20190309329A1/en not_active Abandoned
-
2020
- 2020-05-27 US US16/884,976 patent/US11613767B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US9260729B2 (en) | 2016-02-16 |
| ES2703775T3 (es) | 2019-03-12 |
| CA3081506A1 (en) | 2009-09-11 |
| EP2262901B1 (en) | 2018-11-21 |
| EP2262901A4 (en) | 2014-07-16 |
| US20120040426A1 (en) | 2012-02-16 |
| US20160355845A1 (en) | 2016-12-08 |
| SI2262901T1 (sl) | 2019-02-28 |
| US7977084B2 (en) | 2011-07-12 |
| US10208320B2 (en) | 2019-02-19 |
| JP5755884B2 (ja) | 2015-07-29 |
| CA2717586C (en) | 2020-08-11 |
| US20210108232A1 (en) | 2021-04-15 |
| JP2014100147A (ja) | 2014-06-05 |
| CA2717586A1 (en) | 2009-09-11 |
| BRPI0909690A2 (pt) | 2019-02-26 |
| JP2011512848A (ja) | 2011-04-28 |
| PL2262901T3 (pl) | 2019-04-30 |
| US20090275097A1 (en) | 2009-11-05 |
| ZA201007065B (en) | 2011-07-27 |
| EP3450550A1 (en) | 2019-03-06 |
| US20190309329A1 (en) | 2019-10-10 |
| US11613767B2 (en) | 2023-03-28 |
| WO2009111672A1 (en) | 2009-09-11 |
| EP2262901A1 (en) | 2010-12-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11613767B2 (en) | Primary alcohol producing organisms | |
| US20210040511A1 (en) | Microorganisms for the production of methacrylic acid | |
| EP2185708A1 (en) | Methods and organisms for the growth-coupled production of 1,4-butanediol | |
| WO2012177726A1 (en) | Microorganism for producing primary alcohols and related compounds and methods related thereto | |
| WO2010057022A1 (en) | Microorganisms for the production of methyl ethyl ketone and 2-butanol | |
| EP2909325A1 (en) | Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing succinate related thereto | |
| CN114728871B (zh) | 用于减少副产物的微生物和方法 | |
| Park et al. | Systems biological approach for the production of various polyhydroxyalkanoates by metabolically engineered Escherichia coli | |
| Mehrer | Growth-coupled Metabolic Engineering for High-yield Chemical Production | |
| WO2023178261A1 (en) | Microbial production of z3-hexenol, z3-hexenal and z3-hexenyl acetate | |
| Cardenas et al. | Metabolic engineering of Saccharomyces cerevisiae for the production of triacetic acid | |
| AU2013202923A1 (en) | Microorganism for producing primary alcohols and related compounds and methods related thereto | |
| BR122018011330B1 (pt) | Micro-organismo de ocorrência não-natural e método para a produção de 1,4-butanodiol |