DK175093B1 - DNA-fragment, fremgangsmåde til fremstilling deraf, gærhybridpromotor, indeholdende DNA-fragmentet, fremgangsmåde til fremstilling deraf...... - Google Patents

DNA-fragment, fremgangsmåde til fremstilling deraf, gærhybridpromotor, indeholdende DNA-fragmentet, fremgangsmåde til fremstilling deraf...... Download PDF

Info

Publication number
DK175093B1
DK175093B1 DK198604096A DK409686A DK175093B1 DK 175093 B1 DK175093 B1 DK 175093B1 DK 198604096 A DK198604096 A DK 198604096A DK 409686 A DK409686 A DK 409686A DK 175093 B1 DK175093 B1 DK 175093B1
Authority
DK
Denmark
Prior art keywords
yeast
dna
hybrid
promoter
fragment
Prior art date
Application number
DK198604096A
Other languages
Danish (da)
English (en)
Other versions
DK409686A (da
DK409686D0 (da
Inventor
Albert Hinnen
Bernd Meyhack
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of DK409686D0 publication Critical patent/DK409686D0/da
Publication of DK409686A publication Critical patent/DK409686A/da
Application granted granted Critical
Publication of DK175093B1 publication Critical patent/DK175093B1/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6456Plasminogen activators
    • C12N9/6459Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/65Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/815Protease inhibitors from leeches, e.g. hirudin, eglin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21069Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Alcoholic Beverages (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Led Devices (AREA)
  • Gyroscopes (AREA)
  • Graft Or Block Polymers (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
DK198604096A 1985-08-29 1986-08-28 DNA-fragment, fremgangsmåde til fremstilling deraf, gærhybridpromotor, indeholdende DNA-fragmentet, fremgangsmåde til fremstilling deraf...... DK175093B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8521496 1985-08-29
GB858521496A GB8521496D0 (en) 1985-08-29 1985-08-29 Repressible yeast promoters

Publications (3)

Publication Number Publication Date
DK409686D0 DK409686D0 (da) 1986-08-28
DK409686A DK409686A (da) 1987-03-01
DK175093B1 true DK175093B1 (da) 2004-05-24

Family

ID=10584428

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198604096A DK175093B1 (da) 1985-08-29 1986-08-28 DNA-fragment, fremgangsmåde til fremstilling deraf, gærhybridpromotor, indeholdende DNA-fragmentet, fremgangsmåde til fremstilling deraf......

Country Status (22)

Country Link
US (1) US5436136A (ja)
EP (1) EP0213593B1 (ja)
JP (1) JPH07110233B2 (ja)
AT (1) ATE62510T1 (ja)
AU (1) AU599329B2 (ja)
CA (1) CA1318616C (ja)
DD (2) DD254212A5 (ja)
DE (1) DE3678647D1 (ja)
DK (1) DK175093B1 (ja)
ES (3) ES2001618A6 (ja)
FI (1) FI91280C (ja)
GB (1) GB8521496D0 (ja)
GR (1) GR862209B (ja)
HU (1) HU211207B (ja)
IE (1) IE58844B1 (ja)
IL (1) IL79837A (ja)
NO (1) NO175871C (ja)
NZ (1) NZ217388A (ja)
PH (1) PH24715A (ja)
PT (1) PT83273B (ja)
SU (1) SU1630616A3 (ja)
ZA (1) ZA866535B (ja)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU621277B2 (en) * 1985-11-13 1992-03-12 Xoma Corporation Shortened phosphoglycerate kinase promoter
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
GB8620926D0 (en) * 1986-08-29 1986-10-08 Delta Biotechnology Ltd Yeast promoter
GB8907110D0 (en) * 1988-05-04 1989-05-10 Ciba Geigy Ag Improvements in the production of polypeptides
NO177065C (no) * 1988-09-26 1995-07-12 Labofina Sa Framgangsmåte for framstilling av enzymatisk aktivt humant lysozym
WO1992004363A1 (en) * 1990-09-04 1992-03-19 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
CA2105064A1 (en) * 1991-04-01 1992-10-02 Martin Anthony Gleeson Genes which influence pichia proteolytic activity, and uses therefor
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
JPH08173170A (ja) * 1994-05-25 1996-07-09 Kirin Brewery Co Ltd キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現
ATE284972T1 (de) * 1996-09-24 2005-01-15 Cadus Pharmaceutical Corp Verfahren und zusammensetzungen für die identifizierung von receptor effectoren
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
US6890730B1 (en) 1999-12-10 2005-05-10 The Salk Institute Sequence and method for increasing protein expression in cellular expression systems
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
CN116018350A (zh) 2020-04-20 2023-04-25 韦斯塔隆公司 用于有害生物防治的蛋白水解稳定的U1-漏斗网蛛毒素-Ta1b变体多肽
KR20230005929A (ko) 2020-05-01 2023-01-10 베스타론 코포레이션 살충 조합물
AR125258A1 (es) 2021-04-01 2023-06-28 Vestaron Corp Polipéptidos con mutaciones de av3 para el control de plagas
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
WO2023225555A1 (en) 2022-05-18 2023-11-23 Vestaron Corporation Pesticidal actx peptide variants
WO2023245100A1 (en) 2022-06-17 2023-12-21 Vestaron Corporation Antimicrobial ncr13 variant peptides
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
AU577259B2 (en) * 1982-08-13 1988-09-22 Zymogenetics Inc. Glycolytic promters for regulated protein expression protease inhibitor
US4876197A (en) * 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
IE58011B1 (en) * 1983-05-27 1993-06-16 Texas A & M Univ Sys Method for producing a recombinant baculovirus expression vector
GB8314961D0 (en) * 1983-05-31 1983-07-06 Kingsman A J Dna sequence
EP0143081B1 (en) * 1983-11-21 1989-08-23 Ciba-Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast
ATE102250T1 (de) * 1984-05-11 1994-03-15 Chiron Corp Erhoehte hefetranskription unter verwendung einer hybridkonstruktion der promotorregion.
EP0164566A3 (de) * 1984-05-11 1986-10-29 LGA Gastechnik GmbH Sicherheitseinrichtung für Flüssiggastanks
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen

Also Published As

Publication number Publication date
AU599329B2 (en) 1990-07-19
IE58844B1 (en) 1993-11-17
ES2006382A6 (es) 1989-04-16
EP0213593B1 (en) 1991-04-10
US5436136A (en) 1995-07-25
GB8521496D0 (en) 1985-10-02
PH24715A (en) 1990-10-01
IL79837A (en) 1992-06-21
NZ217388A (en) 1989-02-24
ZA866535B (en) 1987-04-29
DD254212A5 (de) 1988-02-17
PT83273A (en) 1986-09-01
PT83273B (pt) 1989-05-12
DK409686A (da) 1987-03-01
HU211207B (en) 1995-11-28
FI863430A0 (fi) 1986-08-25
GR862209B (en) 1986-12-31
ES2006383A6 (es) 1989-04-16
NO863458L (no) 1987-03-02
FI91280C (fi) 1994-06-10
FI863430A (fi) 1987-03-01
ATE62510T1 (de) 1991-04-15
NO175871C (no) 1994-12-21
IE862307L (en) 1987-02-28
JPS6251995A (ja) 1987-03-06
DD267739A5 (de) 1989-05-10
ES2001618A6 (es) 1988-06-01
NO175871B (ja) 1994-09-12
FI91280B (fi) 1994-02-28
AU6203286A (en) 1987-03-05
CA1318616C (en) 1993-06-01
DK409686D0 (da) 1986-08-28
DE3678647D1 (en) 1991-05-16
EP0213593A1 (en) 1987-03-11
HUT42525A (en) 1987-07-28
JPH07110233B2 (ja) 1995-11-29
NO863458D0 (no) 1986-08-28
SU1630616A3 (ru) 1991-02-23
IL79837A0 (en) 1986-11-30

Similar Documents

Publication Publication Date Title
DK175093B1 (da) DNA-fragment, fremgangsmåde til fremstilling deraf, gærhybridpromotor, indeholdende DNA-fragmentet, fremgangsmåde til fremstilling deraf......
DK174905B1 (da) DNA-sekvens og fremgangsmåde til den fremstilling, gærhybridvektorer og Saccharomyces cerevisiae indeholdende sekvensen henholdsvis vektoren og fremgangsmåder til deres fremstilling samt deres anvendelse ved fremstilling af polypeptider
EP0123544A2 (en) Process for expressing heterologous protein in yeast, expression vehicles and yeast organisms therefor
DK174977B1 (da) Humane t-PA-proteiner, deres fremstilling, DNA, hybridvektor og transformeret eukaryot værtscelle indeholdende en DNA-sekvens, som koder for de humane t-PA-proteiner, og farmaceutisk præparat indeholdende et sådant t-PA-protein
FI90352C (fi) Menetelmä heterologisten proteiinien tuottamiseksi Yarrowia lipolytica -transformanttien avulla, menetelmä Yarrowia lipolytica -transformanttien valmistamiseksi ja toteamiseksi sekä menetelmä transformanttien valmistamiseen käyttökelpoisten plasmidien valmistamiseksi
NO830780L (no) Uttrykking, behandling og sekresjon av heterologt protein av gjaer
JP2865683B2 (ja) 真正のヒト血清アルブミンをコードする人工遺伝子、その使用およびその製造法
DK175492B1 (da) Fremstilling af thrombininhibitorer
DK164941B (da) Gaerhybridvektor, transformeret gaercelle samt fremgangsmaade til fremstilling af vaevsplasminogenaktivitor (tpa)
FI105484B (fi) Pektiinilyaasin ilmentämisjärjestelmää koodittava yhdistelmä-DNA-molekyyli
JPS6236183A (ja) サツカロミセス・セレビシエからのポリペプチドの発現および分泌
FI104637B (fi) Uudet yhdistelmä-DNA-molekyylit sekä bakteeri- ja sieni-isännät yhdistelmäinterferonien ja pektiinilyaasien tuottamiseksi
US5447862A (en) Pectin lyase genes of aspergillus niger
EP0421919A2 (en) Polygalacturonase encoding fungal expression system
JPH03201987A (ja) ヒト血清アルブミン断片
KR940011534B1 (ko) 억제성 효모 프로모터의 제조방법
Curran et al. Biotechnological Exploitation of Heterologous Protein Production in Fungi
CA1335081C (en) Expression system comprising aspergillus niger pectin lyase i gene sequences
Skipper Analysis and utilization of the preprotoxin gene encoded in the M1 double-stranded RNA of yeast
PH26266A (en) Yeast hybrid vectors and their use for the production of polypeptides

Legal Events

Date Code Title Description
PUP Patent expired