DK1088102T3 - PCR-primerkonstruktioner til anvendelse i et integreret signalsystem - Google Patents

PCR-primerkonstruktioner til anvendelse i et integreret signalsystem

Info

Publication number
DK1088102T3
DK1088102T3 DK98955781T DK98955781T DK1088102T3 DK 1088102 T3 DK1088102 T3 DK 1088102T3 DK 98955781 T DK98955781 T DK 98955781T DK 98955781 T DK98955781 T DK 98955781T DK 1088102 T3 DK1088102 T3 DK 1088102T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
template
primer
target
binding region
Prior art date
Application number
DK98955781T
Other languages
English (en)
Inventor
Neil James Gibson
Stephen Little
Jane Theaker
David Mark Whitcombe
Original Assignee
Dxs Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dxs Ltd filed Critical Dxs Ltd
Application granted granted Critical
Publication of DK1088102T3 publication Critical patent/DK1088102T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
DK98955781T 1998-06-13 1998-11-25 PCR-primerkonstruktioner til anvendelse i et integreret signalsystem DK1088102T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9812768.1A GB9812768D0 (en) 1998-06-13 1998-06-13 Methods
PCT/GB1998/003521 WO1999066071A1 (en) 1998-06-13 1998-11-25 Pcr primer constructs for use in an integrated signalling system

Publications (1)

Publication Number Publication Date
DK1088102T3 true DK1088102T3 (da) 2003-11-10

Family

ID=10833706

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98955781T DK1088102T3 (da) 1998-06-13 1998-11-25 PCR-primerkonstruktioner til anvendelse i et integreret signalsystem

Country Status (12)

Country Link
US (3) US6326145B1 (da)
EP (1) EP1088102B8 (da)
JP (1) JP4440469B2 (da)
AT (1) ATE245198T1 (da)
AU (1) AU1250799A (da)
CA (1) CA2377508C (da)
DE (1) DE69816488T2 (da)
DK (1) DK1088102T3 (da)
ES (1) ES2205572T3 (da)
GB (2) GB9812768D0 (da)
PT (1) PT1088102E (da)
WO (1) WO1999066071A1 (da)

Families Citing this family (226)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
DE69937223T3 (de) 1998-11-09 2011-05-05 Eiken Kagaku K.K. Verfahren zur synthese von nukleinsäuren
US6858390B2 (en) 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US6656692B2 (en) 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6403313B1 (en) * 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US6420115B1 (en) 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay
US6900300B1 (en) 2000-09-19 2005-05-31 Ingeneus Corporation Quadruplex DNA and duplex probe systems
US20020025519A1 (en) * 1999-06-17 2002-02-28 David J. Wright Methods and oligonucleotides for detecting nucleic acid sequence variations
US20030165913A1 (en) * 1999-06-17 2003-09-04 Sha-Sha Wang Methods for detecting nucleic acid sequence variations
GB9918237D0 (en) 1999-08-04 1999-10-06 Secr Defence Detection system
EP1231281B1 (en) 1999-11-08 2006-02-01 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
JP4721603B2 (ja) 1999-11-08 2011-07-13 栄研化学株式会社 変異および／または多型の検出方法
US6911536B1 (en) 1999-12-21 2005-06-28 Ingeneus Corporation Triplex and quadruplex catalytic hybridization
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6924108B2 (en) 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US7052844B2 (en) 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6927027B2 (en) 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US6982147B2 (en) 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US7220541B2 (en) 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
US6613524B1 (en) 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
AU2001244677A1 (en) 2000-04-07 2001-10-23 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
US7659054B1 (en) * 2000-05-23 2010-02-09 Nuvelo, Inc. Methods for genetic analysis of DNA to detect sequence variances
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
GB0111275D0 (en) 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
WO2002103052A1 (en) * 2001-06-15 2002-12-27 Quiatech Ab Amplifiable probe
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
CA2467460A1 (en) * 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
AU2003212087B2 (en) * 2002-02-26 2008-06-12 Commonwealth Scientific And Industrial Research Organisation Headloop DNA amplification
AUPS076802A0 (en) * 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
US6818420B2 (en) * 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
JP3867926B2 (ja) * 2002-10-29 2007-01-17 独立行政法人理化学研究所 核酸の増幅法
GB0304832D0 (en) 2003-03-04 2003-04-09 Secr Defence Assay method
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
EP2199406A1 (en) 2003-05-01 2010-06-23 Veridex, LLC Intraoperative lymph node assay
EP1660679B1 (en) * 2003-08-01 2012-11-14 Dynal Biotech Inc. Self-hybridizing multiple target nucleic acid probes and methods of use
DE10338308B4 (de) * 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
ES2383970T3 (es) 2003-10-21 2012-06-27 Orion Genomics, Llc Fragmentación enzimática diferencial
WO2005063977A1 (ja) * 2003-12-25 2005-07-14 Riken 核酸の増幅法およびこれを利用した変異核酸の検出法
WO2005086647A2 (en) * 2004-02-23 2005-09-22 University Of Maryland, Baltimore Immuno-pcr method for the detection of a biomolecule in a test sample
GB0406015D0 (en) * 2004-03-17 2004-04-21 Dynal Biotech Asa Improvements in magnetic polymer particles
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
JP2008502330A (ja) * 2004-06-04 2008-01-31 ベリデックス・エルエルシー 乳癌の診断または経過の予測
US20070154889A1 (en) * 2004-06-25 2007-07-05 Veridex, Llc Methods and reagents for the detection of melanoma
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US8883487B2 (en) 2004-12-23 2014-11-11 Abbott Point Of Care Inc. Molecular diagnostics system and methods
GB0503172D0 (en) 2005-02-16 2005-03-23 Enigma Diagnostics Ltd Detection method
EP2333561A3 (en) 2005-03-10 2014-06-11 Gen-Probe Incorporated System for performing multi-formatted assays
GB2424886A (en) * 2005-04-04 2006-10-11 Dxs Ltd Polynucleotide primers against epidermal growth factor receptor and method of detecting gene mutations
JP5133238B2 (ja) 2005-04-15 2013-01-30 エピゲノミックス アクチェンゲゼルシャフト 遠隔サンプル由来のｄｎａ断片を提供する方法
WO2006122208A1 (en) * 2005-05-11 2006-11-16 Stratagene California Snapback oligonucleotide probe
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
GB0517005D0 (en) 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
US20070059753A1 (en) 2005-09-15 2007-03-15 Tatiana Vener Detecting gene methylation
NZ566387A (en) * 2005-10-05 2010-05-28 Astrazeneca Uk Ltd Method to predict or monitor the response of a patient to an ErbB receptor drug by screening for mutations in an ErbB receptor
GB2432365A (en) * 2005-11-18 2007-05-23 Dxs Ltd A nucleic acid molecule for detecting polymorphisms in the UGT1A1 promoter
CA2631881C (en) 2005-12-07 2015-03-24 Tibotec Pharmaceuticals Ltd. Methods, plasmid vectors and primers for assessing hiv viral fitness
GB0605584D0 (en) * 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
JP2009536825A (ja) * 2006-05-11 2009-10-22 ジーンオーム サイエンシーズ、インク． 可変ゲノムのための１００％配列一致検出方法
JP2009537152A (ja) 2006-05-17 2009-10-29 カリフォルニア インスティテュート オブ テクノロジー 温度サイクルシステム
US8076067B2 (en) * 2006-08-15 2011-12-13 Genetag Technology, Inc. Probe-antiprobe compositions and methods for DNA or RNA detection
US8148511B2 (en) * 2006-09-28 2012-04-03 The University Of North Carolina At Chapel Hill Methods and compositions for the detection and quantification of E. coli and Enterococcus
IL186980A0 (en) 2006-10-31 2008-02-09 Veridex Llc Characterizing prostate cancer
IL186935A0 (en) 2006-10-31 2008-02-09 Veridex Llc Prostate cancer field effect analysis methods and kits
US20100003670A1 (en) * 2006-10-31 2010-01-07 Haiying Wang Characterizing prostate cancer
US7955802B2 (en) 2006-12-13 2011-06-07 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US20100129792A1 (en) * 2007-02-06 2010-05-27 Gerassimos Makrigiorgos Direct monitoring and pcr amplification of the dosage and dosage difference between target genetic regions
AU2008237203B2 (en) 2007-04-06 2014-08-14 Becton, Dickinson And Company Compositions and methods for the identification of a carbapenemase gene
US20080254455A1 (en) 2007-04-12 2008-10-16 Haiying Wang Detecting prostate cancer
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US8999634B2 (en) 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
EP2152902A2 (en) 2007-06-08 2010-02-17 Epigenomics AG Method for methylation analysis
EP2167964B1 (de) * 2007-06-13 2015-03-11 Attomol GmbH Molekulare Diagnostika Verfahren zur durchführung und auswertung von mix&measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat
WO2009021054A2 (en) 2007-08-06 2009-02-12 Orion Genomics Llc Novel single nucleotide polymorphisms and combinations of novel and known polymorphisms for determining the allele-specific expression of the igf2 gene
CA2696433A1 (en) * 2007-08-13 2009-03-12 3M Innovative Properties Company Methods for detecting drug-resistant microbes
GB2453173A (en) * 2007-09-28 2009-04-01 Dxs Ltd Polynucleotide primers
CA2705213C (en) 2007-11-07 2016-10-04 The University Of British Columbia Microfluidic device and method of using same
US20090197254A1 (en) * 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
DE102007063102B4 (de) * 2007-12-28 2022-02-10 Robert Bosch Gmbh Verfahren zur Erfassung eines periodisch pulsierenden Betriebsparameters
DE102008005667A1 (de) * 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
UA103319C2 (en) 2008-05-06 2013-10-10 Глаксосмитклайн Ллк Thiazole- and oxazole-benzene sulfonamide compounds
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
EP2147981A1 (en) 2008-07-25 2010-01-27 Biotype AG Kit and method for evaluating detection properties in amplification reactions
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
WO2010067055A1 (en) 2008-12-09 2010-06-17 Oxitec Ltd. Enhanced taqman probe based amplification
DE102008064164A1 (de) * 2008-12-22 2010-07-01 Leica Microsystems Cms Gmbh Verfahren zur räumlich hochauflösenden stochastischen Untersuchung einer mit einer Substanz markierten Struktur einer Probe
CA2751758A1 (en) 2009-02-11 2010-08-19 Orion Genomics Llc Combinations of polymorphisms for determining allele-specific expression of igf2
WO2010101947A2 (en) * 2009-03-02 2010-09-10 Cooperative Diagnostics, Llc Rapid oligo probes
JP5687414B2 (ja) 2009-04-20 2015-03-18 オリンパス株式会社 多型の識別方法
AU2010248782B2 (en) * 2009-05-15 2015-06-11 Insight Genetics, Inc. Methods and compositions relating to fusions of ALK for diagnosing and treating cancer
EP2443256A1 (en) 2009-06-15 2012-04-25 BG Research Ltd Nucleic acid detection
US9587270B2 (en) * 2009-06-29 2017-03-07 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
WO2011011391A1 (en) 2009-07-22 2011-01-27 E.I. Dupont De Nemours And Company Sequences and their use for detection and characterization of e. coli o157:h7
AU2010315399B2 (en) 2009-10-27 2016-01-28 Swift Biosciences, Inc. Bimolecular primers
KR20110050327A (ko) 2009-11-07 2011-05-13 주식회사 씨젠 Ｔｈｄ 프라이머 타겟 검출
JP2013511285A (ja) * 2009-11-23 2013-04-04 スイフト・バイオサイエンシズ・インコーポレイテツド 一本鎖標的分子を伸長させるデバイス
WO2011078441A1 (en) 2009-12-21 2011-06-30 Seegene, Inc. Tsg primer target detection
CN102762745A (zh) * 2009-12-24 2012-10-31 Seegene株式会社 排除错误信号的靶核酸序列的实时多路检测
US20110177512A1 (en) * 2010-01-19 2011-07-21 Predictive Biosciences, Inc. Method for assuring amplification of an abnormal nucleic acid in a sample
EP2534263B1 (en) * 2010-02-09 2020-08-05 Unitaq Bio Methods and compositions for universal detection of nucleic acids
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ， インコーポレイテッド デジタル検体分析
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
GB201004292D0 (en) * 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
CN102939534A (zh) 2010-04-02 2013-02-20 维里德克斯有限责任公司 用于临床局限性前列腺癌患者的基于基因的psa复发预测
JP2013529089A (ja) 2010-06-07 2013-07-18 エフ．ホフマン−ラ ロシュ アーゲー インターロイキン−６受容体を阻害するモノクローナル抗体による薬剤治療に対する応答を予測するための遺伝子発現マーカー
GB201011971D0 (en) 2010-07-15 2010-09-01 Olink Ab Methods and product
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
US20120070829A1 (en) 2010-09-10 2012-03-22 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
WO2012049316A1 (en) 2010-10-15 2012-04-19 Olink Ab Dynamic range methods
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
CN103228785B (zh) * 2010-11-24 2016-09-07 株式会社钟化 扩增核酸的检测方法和检测装置
EP2658994B1 (en) 2010-12-28 2017-07-19 Qiagen Hamburg GmbH Oligonucleotide probe for the detection of adenovirus
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
EP2675913B1 (en) 2011-02-15 2016-12-14 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US8718948B2 (en) 2011-02-24 2014-05-06 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
KR101569479B1 (ko) 2011-03-29 2015-11-16 주식회사 씨젠 Ｐｔｏ 절단 및 연장-의존적 절단에 의한 타겟 핵산서열의 검출
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
MX342067B (es) 2011-05-04 2016-09-09 Seegene Inc Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
JP5863162B2 (ja) * 2011-06-17 2016-02-16 広島県 標的核酸の検出方法
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2756101B1 (en) 2011-09-15 2018-05-23 David A. Shafer Probe: antiprobe compositions for high specificity dna or rna detection
BR112014007394A2 (pt) 2011-09-28 2018-08-28 Du Pont método para detectar a presença de bactérias, polinucleotídeo isolado, kit e comprimido reagente
WO2013070662A1 (en) 2011-11-09 2013-05-16 E.I. Du Pont De Nemours And Company Sequences and their use for detection of salmonella
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
US20150126438A1 (en) 2012-01-24 2015-05-07 Beth Israel Deaconess Medical Center, Inc. Novel ChREBP Isoforms and Methods Using the Same
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
CA2864523C (en) 2012-03-05 2019-02-05 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay
US20150031577A1 (en) * 2012-03-20 2015-01-29 UNIVERSITé LAVAL Nucleic acid detection method comprising target specific indexing probes (tsip) comprising a releasable segment to be detected via fluorescence when bound to a capture probe
WO2013152307A1 (en) 2012-04-05 2013-10-10 The Regents Of The University Of California Gene expression panel for breast cancer prognosis
CN104919057B (zh) 2012-11-14 2018-09-25 欧凌科公司 基于rca的局部扩增方法
CN104955963A (zh) 2012-11-14 2015-09-30 欧凌科公司 Rca报告探针及其用于检测核酸分子的用途
KR101760267B1 (ko) 2012-12-27 2017-07-24 주식회사 씨젠 Pto 절단 및 연장-의존적 비-혼성화 어세이에 의한 타겟 핵산서열의 검출
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
WO2014121091A1 (en) 2013-02-01 2014-08-07 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
WO2014130416A1 (en) * 2013-02-21 2014-08-28 E. I. Du Pont De Nemours And Company Sequences and their use for detection and characterization of e. coli o157:h7
AU2014200958B2 (en) 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
WO2014138119A2 (en) 2013-03-04 2014-09-12 Fry Laboratories, LLC Method and kit for characterizing microorganisms
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
US9347095B2 (en) 2013-03-15 2016-05-24 Bio-Rad Laboratories, Inc. Digital assays for mutation detection
KR20220100079A (ko) 2013-03-15 2022-07-14 아벨리노 랩 유에스에이, 인크. 대립유전자 검출을 위한 게놈 dna 주형의 개선된 단리 방법
WO2014160797A1 (en) 2013-03-26 2014-10-02 Genetag Technology, Inc. Dual probe:antiprobe compositions for dna and rna detection
WO2014189843A1 (en) 2013-05-20 2014-11-27 Board Of Trustees Of The University Of Arkansas Gep5 model for multiple myeloma
EP3022319B1 (en) 2013-07-15 2023-05-17 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
EP4321867A3 (en) * 2013-08-09 2024-05-15 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
US20160201116A1 (en) 2013-08-20 2016-07-14 E. I. Du Pont De Nemours And Company Sequences and their use for detection of salmonella enteritidis and/or salmonella typhimurium
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
CN105705657B (zh) 2013-10-18 2020-07-28 Seegene株式会社 基于利用杂交-捕捉和模板化寡核苷酸的探测和标记寡核苷酸切割及延伸分析的在固相中的靶核酸序列检测
GB201320145D0 (en) 2013-11-14 2014-01-01 Olink Ab Localised RCA-based amplification method using a padlock-probe
TWI755350B (zh) 2013-11-15 2022-02-21 美商阿韋利諾美國實驗有限公司 用於多工偵測與眼科病況有關的對偶基因之方法
WO2015085230A1 (en) 2013-12-06 2015-06-11 Bio-Rad Laboratories, Inc. Fusion polymerases
CA2933387C (en) 2013-12-11 2023-05-02 The Regents Of The University Of California Methods for labeling dna fragments to reconstruct physical linkage and phase
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015095047A1 (en) * 2013-12-16 2015-06-25 E. I. Du Pont De Nemours Sequences and their use for detection of genus listeria species
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP3172332B1 (en) 2014-07-22 2020-10-14 Bio-rad Laboratories, Inc. Buffers for use with polymerases
EP3174980A4 (en) 2014-08-01 2018-01-17 Dovetail Genomics, LLC Tagging nucleic acids for sequence assembly
WO2016086004A1 (en) * 2014-11-26 2016-06-02 Cascade Biosystems, Inc. Detecting nucleic acid
EP3259696A1 (en) 2015-02-17 2017-12-27 Dovetail Genomics LLC Nucleic acid sequence assembly
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
CN104845967B (zh) 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
ES2961374T3 (es) 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
JP6956012B2 (ja) 2015-05-18 2021-10-27 サガ ダイアグノスティクス アーベー 標的核酸及び多様体の検出
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
CN108368542B (zh) 2015-10-19 2022-04-08 多弗泰尔基因组学有限责任公司 用于基因组组装、单元型定相以及独立于靶标的核酸检测的方法
GB201518655D0 (en) 2015-10-21 2015-12-02 Olink Ab Method for generating proximity probes
WO2017105104A1 (en) 2015-12-15 2017-06-22 Seegene, Inc. Signal extraction for a target nucleic acid sequence
EP4219749A3 (en) 2015-12-30 2023-08-16 Bio-Rad Laboratories, Inc. Split-cycle and tape amplification
AU2017223600B2 (en) 2016-02-23 2023-08-03 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing
WO2017189794A1 (en) * 2016-04-27 2017-11-02 President And Fellows Of Harvard College Method of secure communication via nucleotide polymers
WO2017197300A1 (en) 2016-05-13 2017-11-16 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
MA54568A (fr) 2016-07-01 2021-10-27 Carlsberg As Substitutions de nucléotides prédéterminées
AU2017301881A1 (en) 2016-07-29 2019-02-07 Juno Therapeutics, Inc. Methods for assessing the presence or absence of replication competent virus
GB201621514D0 (en) 2016-12-16 2017-02-01 Q-Linea Ab Padlock probe detection method
CA3046303A1 (en) 2016-12-29 2018-07-05 Seegene, Inc. Method for reducing primer dimer formation and increasing amplification efficiency
JP7113842B2 (ja) 2017-03-29 2022-08-05 クラウン バイオサイエンス，インコーポレイテッド（タイツァン） 胃がんのセツキシマブ感受性を決定するためのシステムおよび方法
AU2018258580A1 (en) 2017-04-28 2019-11-21 Avellino Lab Usa, Inc. Methods for detecting alleles associated with keratoconus
CA3067602A1 (en) 2017-06-29 2019-01-03 Juno Therapeutics, Inc. Mouse model for assessing toxicities associated with immunotherapies
WO2019027850A1 (en) 2017-07-29 2019-02-07 Juno Therapeutics, Inc. CELL EXPANSION REAGENTS EXPRESSING RECOMBINANT RECEPTORS
KR102380264B1 (ko) 2017-08-31 2022-03-29 주식회사 씨젠 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가
EP3676403A1 (en) 2017-09-01 2020-07-08 Juno Therapeutics, Inc. Gene expression and assessment of risk of developing toxicity following cell therapy
EP3688188A4 (en) 2017-09-29 2021-06-16 Seegene, Inc. DETECTION OF TARGET NUCLEAR ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION DEPENDENT NON-HYBRIDIZATION TEST
US11597965B2 (en) 2017-10-06 2023-03-07 10X Genomics, Inc. RNA templated ligation
US20210254000A1 (en) 2017-11-01 2021-08-19 Juno Therapeutics, Inc. Process for producing a t cell composition
WO2019135567A1 (en) 2018-01-05 2019-07-11 Seegene, Inc. . Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
US11535903B2 (en) 2018-01-31 2022-12-27 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
US20210324452A1 (en) 2018-09-06 2021-10-21 Hygiena, Llc Sequences and their use for detection and characterization of escherichia coli serotype o157:h7
KR102220701B1 (ko) 2018-09-28 2021-03-03 (주)나노헬릭스 형광기반의 다중 융해 분석을 이용한 증폭 산물의 다중 분석방법
CA3117720A1 (en) 2018-11-06 2020-05-14 Juno Therapeutics, Inc. Process for producing genetically engineered t cells
KR102097721B1 (ko) 2019-01-24 2020-04-06 주식회사 시선바이오머티리얼스 태그서열 snp를 이용한 단일 검출 프로브 기반 다중 표적 검출방법
US11634782B2 (en) 2019-03-20 2023-04-25 Hygiena, Llc Quantification of microorganisms in samples and methods of determining quantification conditions thereof
WO2020205491A1 (en) 2019-04-05 2020-10-08 Hygiena, Llc Sequences and their use for detection and characterization of genus cronobacter
JP2022529059A (ja) 2019-04-17 2022-06-16 アルパイン イミューン サイエンシズ インコーポレイテッド バリアントｉｃｏｓリガンド（ｉｃｏｓｌ）融合タンパク質の方法および使用
SG11202113356XA (en) 2019-06-12 2021-12-30 Juno Therapeutics Inc Combination therapy of a cell-mediated cytotoxic therapy and an inhibitor of a prosurvival bcl2 family protein
US20220180971A1 (en) 2019-06-14 2022-06-09 Seegene, Inc. Computer-implemented method for collaborative development of reagents for detection of target nucleic acids
KR20220066892A (ko) 2019-08-22 2022-05-24 주노 쎄러퓨티크스 인코퍼레이티드 Ｔ 세포 요법 및 제스트 동족체 2의 인핸서 (ｅｚｈ2) 억제제의 병용 요법 및 관련 방법
CN114786473B (zh) * 2019-10-10 2023-12-19 嘉士伯有限公司 制备突变植物的方法
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
GB201919029D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
IL294565A (en) 2020-01-13 2022-09-01 Aspen Neuroscience Inc Method for Differentiating Neurons and Related Compositions and Methods of Use
AU2021236383A1 (en) 2020-03-13 2022-09-22 Uh-Oh Labs Inc. Looped primer and loop-de-loop method for detecting target nucleic acid
WO2021231661A2 (en) 2020-05-13 2021-11-18 Juno Therapeutics, Inc. Process for producing donor-batched cells expressing a recombinant receptor
EP4171616A1 (en) 2020-06-26 2023-05-03 Juno Therapeutics GmbH Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
WO2023004371A1 (en) 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
US20230377685A1 (en) 2022-04-15 2023-11-23 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
WO2023230581A1 (en) 2022-05-25 2023-11-30 Celgene Corporation Methods of manufacturing t cell therapies

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1273552A (en) 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
DE3785591T2 (de) 1986-01-10 1993-09-02 Amoco Corp Kompetitiver homogener test.
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
GB8920097D0 (en) 1989-09-06 1989-10-18 Ici Plc Amplification processes
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5612199A (en) * 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
CA2123133C (en) 1991-11-07 2005-01-04 Michael J. Heller Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
EP0566751B1 (en) 1992-03-23 1996-01-10 F. Hoffmann-La Roche Ag DNA detection method
EP0601889A2 (en) 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nucleic acid probes
US5593840A (en) * 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
AU3933393A (en) 1993-03-17 1994-10-11 City Of Hope Nucleic acid sequences having single stranded ends
ATE545711T1 (de) 1993-11-12 2012-03-15 Phri Properties Inc Hybridisierungssonden zur nukleinsäuredetektion sowie universelle stämme, verfahren und kits
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AU693023B2 (en) 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
WO1998010096A1 (en) 1996-04-12 1998-03-12 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
GB9609441D0 (en) * 1996-05-04 1996-07-10 Zeneca Ltd Process
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US5814500A (en) 1996-10-31 1998-09-29 The Johns Hopkins University School Of Medicine Delivery construct for antisense nucleic acids and methods of use
AU7847798A (en) * 1996-12-13 1998-07-03 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Fluorescent nucleotide analog hairpin formation for detection of nuclei c acid hybridization
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5928869A (en) 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5935791A (en) 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
AU753191B2 (en) * 1997-11-25 2002-10-10 Exact Sciences Corporation Devices and methods for detecting target molecules in biological samples
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences

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