ES2177590T3 - Deteccion de amplificacion de acidos nucleicos. - Google Patents

Deteccion de amplificacion de acidos nucleicos.

Info

Publication number
ES2177590T3
ES2177590T3 ES95104931T ES95104931T ES2177590T3 ES 2177590 T3 ES2177590 T3 ES 2177590T3 ES 95104931 T ES95104931 T ES 95104931T ES 95104931 T ES95104931 T ES 95104931T ES 2177590 T3 ES2177590 T3 ES 2177590T3
Authority
ES
Spain
Prior art keywords
amplification
target sequence
products
detection
primer extension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95104931T
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English (en)
Inventor
James Gregory Nadeau
George Terrance Walker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
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Becton Dickinson and Co
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Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2177590T3 publication Critical patent/ES2177590T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Electrophonic Musical Instruments (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Prostheses (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)

Abstract

METODOS PARA DETECTAR, INMOVILIZAR O LOCALIZAR LOS PRODUCTOS DE EXTENSION DE IMPRIMADORES DE UNA REACCION DE AMPLIFICACION POR DESPLAZAMIENTO DE ESPECIES QUE SE ENCUENTRAN ACOPLADOS A, E INDICAN, UNA AMPLIFICACION DE LA SECUENCIA OBJETIVO. LOS PRODUCTOS DE EXTENSION IMPRIMADORES SON PRODUCTOS DE ADN ESPECIFICOS DEL OBJETIVO, SECUNDARIOS GENERADOS JUNTO CON EL SDA DE LA SECUENCIA OBJETIVO Y POR TANTEO SE PUEDEN UTILIZAR PARA DETECTAR Y/O MEDIR LA AMPLIFICACION DE UNA SECUENCIA OBJETIVO EN TIEMPO REAL. EN GENERAL LOS PRODUCTOS DE AMPLIFICACION SECUNDARIOS NO SE PUEDEN AMPLIFICAR Y PERMANECEN INERTES EN LA REACCION DE SDA TRAS SU FORMACION SIN INTERFERIR EN LA AMPLIFICACION DE LA SECUENCIA OBJETIVO. LOS PRODUCTOS DE AMPLIFICACION SECUNDARIOS SE PUEDEN DISEÑAR O MODIFICAR DE MODO QUE CONTENGAN CARACTERISTICAS ESPECIALES PARA FACILITAR SU DETECCION, INMOVILIZACION O LOCALIZACION.
ES95104931T 1994-04-18 1995-04-03 Deteccion de amplificacion de acidos nucleicos. Expired - Lifetime ES2177590T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/229,281 US5547861A (en) 1994-04-18 1994-04-18 Detection of nucleic acid amplification

Publications (1)

Publication Number Publication Date
ES2177590T3 true ES2177590T3 (es) 2002-12-16

Family

ID=22860539

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95104931T Expired - Lifetime ES2177590T3 (es) 1994-04-18 1995-04-03 Deteccion de amplificacion de acidos nucleicos.

Country Status (12)

Country Link
US (3) US5547861A (es)
EP (1) EP0678582B1 (es)
JP (1) JP2674737B2 (es)
KR (1) KR0145908B1 (es)
AT (1) ATE220724T1 (es)
AU (1) AU685903B2 (es)
BR (1) BR9501582A (es)
CA (1) CA2145576C (es)
DE (1) DE69527392T2 (es)
ES (1) ES2177590T3 (es)
SG (1) SG34216A1 (es)
TW (1) TW306977B (es)

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JP2674737B2 (ja) 1997-11-12
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AU1502395A (en) 1995-10-26
SG34216A1 (en) 1996-12-06
DE69527392T2 (de) 2003-03-06
KR0145908B1 (ko) 1998-08-01
US5593867A (en) 1997-01-14
CA2145576A1 (en) 1995-10-19
TW306977B (es) 1997-06-01
EP0678582B1 (en) 2002-07-17
CA2145576C (en) 1998-06-30
BR9501582A (pt) 1997-09-16
USRE39885E1 (en) 2007-10-16
DE69527392D1 (de) 2002-08-22
US5547861A (en) 1996-08-20
EP0678582A1 (en) 1995-10-25

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