DE69929445D1 - Methoden und reagenzien zur erhaltung der rna in zellen und geweben - Google Patents

Methoden und reagenzien zur erhaltung der rna in zellen und geweben

Info

Publication number
DE69929445D1
DE69929445D1 DE69929445T DE69929445T DE69929445D1 DE 69929445 D1 DE69929445 D1 DE 69929445D1 DE 69929445 T DE69929445 T DE 69929445T DE 69929445 T DE69929445 T DE 69929445T DE 69929445 D1 DE69929445 D1 DE 69929445D1
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DE
Germany
Prior art keywords
rna
reagents
methods
conservation
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69929445T
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English (en)
Other versions
DE69929445T2 (de
Inventor
S Lader
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Ambion Inc
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Ambion Inc
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Publication date
Application filed by Ambion Inc filed Critical Ambion Inc
Publication of DE69929445D1 publication Critical patent/DE69929445D1/de
Application granted granted Critical
Publication of DE69929445T2 publication Critical patent/DE69929445T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69929445T 1998-07-31 1999-07-30 Methoden und reagenzien zur erhaltung der rna in zellen und geweben Expired - Lifetime DE69929445T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/127,435 US6204375B1 (en) 1998-07-31 1998-07-31 Methods and reagents for preserving RNA in cell and tissue samples
US127435 1998-07-31
PCT/US1999/017375 WO2000006780A1 (en) 1998-07-31 1999-07-30 Methods and reagents for preserving rna in cell and tissue samples

Publications (2)

Publication Number Publication Date
DE69929445D1 true DE69929445D1 (de) 2006-04-06
DE69929445T2 DE69929445T2 (de) 2006-09-14

Family

ID=22430096

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69942351T Expired - Lifetime DE69942351D1 (de) 1998-07-31 1999-07-30 Methoden und Reagenzien zur Erhaltung der RNA in Zellen und Geweben
DE69929445T Expired - Lifetime DE69929445T2 (de) 1998-07-31 1999-07-30 Methoden und reagenzien zur erhaltung der rna in zellen und geweben

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69942351T Expired - Lifetime DE69942351D1 (de) 1998-07-31 1999-07-30 Methoden und Reagenzien zur Erhaltung der RNA in Zellen und Geweben

Country Status (10)

Country Link
US (6) US6204375B1 (de)
EP (2) EP1019545B1 (de)
JP (1) JP4554080B2 (de)
AT (2) ATE466957T1 (de)
AU (1) AU745943B2 (de)
CA (1) CA2298841C (de)
DE (2) DE69942351D1 (de)
DK (1) DK1019545T3 (de)
ES (1) ES2255293T3 (de)
WO (1) WO2000006780A1 (de)

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