DE69728141T2 - Verfahren zur herstellung eines porösen vernetzten polysaccharidgels und seine verwendung als filtermedien und in der chromatographie - Google Patents
Verfahren zur herstellung eines porösen vernetzten polysaccharidgels und seine verwendung als filtermedien und in der chromatographie Download PDFInfo
- Publication number
- DE69728141T2 DE69728141T2 DE69728141T DE69728141T DE69728141T2 DE 69728141 T2 DE69728141 T2 DE 69728141T2 DE 69728141 T DE69728141 T DE 69728141T DE 69728141 T DE69728141 T DE 69728141T DE 69728141 T2 DE69728141 T2 DE 69728141T2
- Authority
- DE
- Germany
- Prior art keywords
- gel
- polysaccharide
- steps
- solution
- crosslinking agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 79
- 238000004587 chromatography analysis Methods 0.000 title claims description 4
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 150000004676 glycans Chemical class 0.000 claims abstract description 78
- 239000005017 polysaccharide Substances 0.000 claims abstract description 78
- 238000000034 method Methods 0.000 claims abstract description 53
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 35
- 238000004132 cross linking Methods 0.000 claims abstract description 24
- 230000008569 process Effects 0.000 claims abstract description 22
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims abstract description 20
- 239000006185 dispersion Substances 0.000 claims abstract description 17
- 238000007796 conventional method Methods 0.000 claims abstract 8
- 239000000499 gel Substances 0.000 claims description 118
- 239000000243 solution Substances 0.000 claims description 66
- 229920000936 Agarose Polymers 0.000 claims description 41
- 239000002245 particle Substances 0.000 claims description 40
- STMDPCBYJCIZOD-UHFFFAOYSA-N 2-(2,4-dinitroanilino)-4-methylpentanoic acid Chemical compound CC(C)CC(C(O)=O)NC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O STMDPCBYJCIZOD-UHFFFAOYSA-N 0.000 claims description 15
- 230000006855 networking Effects 0.000 claims description 15
- -1 allyl halide Chemical class 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 5
- AFOSIXZFDONLBT-UHFFFAOYSA-N divinyl sulfone Chemical compound C=CS(=O)(=O)C=C AFOSIXZFDONLBT-UHFFFAOYSA-N 0.000 claims description 4
- LIHMQSPBDBGMJW-UHFFFAOYSA-N 1,1-dibromopropan-1-ol Chemical compound CCC(O)(Br)Br LIHMQSPBDBGMJW-UHFFFAOYSA-N 0.000 claims description 3
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 3
- 238000001042 affinity chromatography Methods 0.000 claims description 3
- 230000001804 emulsifying effect Effects 0.000 claims description 3
- 238000002523 gelfiltration Methods 0.000 claims description 2
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims description 2
- 238000004255 ion exchange chromatography Methods 0.000 claims description 2
- 238000004366 reverse phase liquid chromatography Methods 0.000 claims description 2
- BHELZAPQIKSEDF-UHFFFAOYSA-N allyl bromide Chemical compound BrCC=C BHELZAPQIKSEDF-UHFFFAOYSA-N 0.000 claims 1
- 239000013522 chelant Substances 0.000 claims 1
- 230000003213 activating effect Effects 0.000 abstract description 3
- 230000001588 bifunctional effect Effects 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 41
- 239000012153 distilled water Substances 0.000 description 35
- 238000006243 chemical reaction Methods 0.000 description 24
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- 239000007863 gel particle Substances 0.000 description 15
- 239000011734 sodium Substances 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- 239000011148 porous material Substances 0.000 description 11
- 238000003756 stirring Methods 0.000 description 11
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 10
- 239000011159 matrix material Substances 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 229920000642 polymer Polymers 0.000 description 7
- 239000011543 agarose gel Substances 0.000 description 6
- 238000005937 allylation reaction Methods 0.000 description 6
- 238000004945 emulsification Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 5
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 5
- 239000004280 Sodium formate Substances 0.000 description 5
- 230000031709 bromination Effects 0.000 description 5
- 238000005893 bromination reaction Methods 0.000 description 5
- 238000012856 packing Methods 0.000 description 5
- 239000001632 sodium acetate Substances 0.000 description 5
- 235000017281 sodium acetate Nutrition 0.000 description 5
- HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 description 5
- 235000019254 sodium formate Nutrition 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 4
- 239000004971 Cross linker Substances 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 238000001879 gelation Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 108090001008 Avidin Proteins 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 102000008857 Ferritin Human genes 0.000 description 2
- 108050000784 Ferritin Proteins 0.000 description 2
- 238000008416 Ferritin Methods 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 102000004856 Lectins Human genes 0.000 description 2
- 108090001090 Lectins Proteins 0.000 description 2
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 108010034949 Thyroglobulin Proteins 0.000 description 2
- 102000009843 Thyroglobulin Human genes 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000002523 lectin Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 238000001308 synthesis method Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 229960002175 thyroglobulin Drugs 0.000 description 2
- SLBOQBILGNEPEB-UHFFFAOYSA-N 1-chloroprop-2-enylbenzene Chemical compound C=CC(Cl)C1=CC=CC=C1 SLBOQBILGNEPEB-UHFFFAOYSA-N 0.000 description 1
- 101710134784 Agnoprotein Proteins 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 102000028555 IgG binding proteins Human genes 0.000 description 1
- 108091009325 IgG binding proteins Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- CNCOEDDPFOAUMB-UHFFFAOYSA-N N-Methylolacrylamide Chemical compound OCNC(=O)C=C CNCOEDDPFOAUMB-UHFFFAOYSA-N 0.000 description 1
- ABLZXFCXXLZCGV-UHFFFAOYSA-N Phosphorous acid Chemical compound OP(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004820 halides Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- JGJLWPGRMCADHB-UHFFFAOYSA-N hypobromite Inorganic materials Br[O-] JGJLWPGRMCADHB-UHFFFAOYSA-N 0.000 description 1
- 229920000592 inorganic polymer Polymers 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 125000005647 linker group Chemical group 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 150000002924 oxiranes Chemical class 0.000 description 1
- 238000003921 particle size analysis Methods 0.000 description 1
- ZJAOAACCNHFJAH-UHFFFAOYSA-N phosphonoformic acid Chemical compound OC(=O)P(O)(O)=O ZJAOAACCNHFJAH-UHFFFAOYSA-N 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 150000003333 secondary alcohols Chemical class 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000012258 stirred mixture Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 150000003509 tertiary alcohols Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Chemical group 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/291—Gel sorbents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
- B01J20/267—Cross-linked polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
- B01J20/285—Porous sorbents based on polymers
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9601368 | 1996-04-11 | ||
| SE9601368A SE9601368D0 (sv) | 1996-04-11 | 1996-04-11 | Process for the production of a porous cross-linked polysaccharide gel |
| PCT/SE1997/000578 WO1997038018A1 (en) | 1996-04-11 | 1997-04-07 | Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69728141D1 DE69728141D1 (de) | 2004-04-22 |
| DE69728141T2 true DE69728141T2 (de) | 2005-02-17 |
Family
ID=20402148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69728141T Expired - Lifetime DE69728141T2 (de) | 1996-04-11 | 1997-04-07 | Verfahren zur herstellung eines porösen vernetzten polysaccharidgels und seine verwendung als filtermedien und in der chromatographie |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6602990B1 (index.php) |
| EP (1) | EP0892815B1 (index.php) |
| JP (1) | JP4081143B2 (index.php) |
| AT (1) | ATE261992T1 (index.php) |
| AU (1) | AU726086B2 (index.php) |
| DE (1) | DE69728141T2 (index.php) |
| DK (1) | DK0892815T3 (index.php) |
| ES (1) | ES2217413T3 (index.php) |
| SE (1) | SE9601368D0 (index.php) |
| WO (1) | WO1997038018A1 (index.php) |
Families Citing this family (88)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5998606A (en) * | 1997-11-10 | 1999-12-07 | Grandics; Peter | Mn(IV)-mediated crosslinking and functionalization of chromatography media |
| DE19803415A1 (de) * | 1998-01-29 | 1999-08-05 | Aventis Res & Tech Gmbh & Co | Trennung von Stoffgemischen unter Einsatz von Polysacchariden |
| SE9901825D0 (sv) | 1999-05-20 | 1999-05-20 | Amersham Pharm Biotech Ab | Foamed material filled with inner material |
| SE0004928D0 (sv) * | 2000-12-29 | 2000-12-29 | Apbiotech Ab | A method for the manufacturing of porous material |
| SE0004929D0 (sv) * | 2000-12-29 | 2000-12-29 | Apbiotech Ab | A method for producing liquid chromatography matrices |
| KR101013252B1 (ko) * | 2003-03-26 | 2011-02-09 | 나고야 인더스트리얼 사이언스 리서치 인스티튜트 | 크로마토그래피용 분리제 및 그의 제조 방법 |
| SE0400490D0 (sv) | 2004-02-26 | 2004-02-26 | Amersham Biosciences Ab | Plasmid purification |
| ES2608005T3 (es) * | 2004-09-22 | 2017-04-05 | Ge Healthcare Bioprocess R&D Ab | Método para preparar una matriz cromatográfica |
| SE0402322D0 (sv) * | 2004-09-22 | 2004-09-22 | Amersham Biosciences Ab | Method of preparing a chromatography matrix |
| PL1827691T3 (pl) * | 2004-10-21 | 2017-07-31 | Ge Healthcare Bioprocess R&D Ab | Matryca chromatograficzna |
| WO2006065208A1 (en) * | 2004-12-14 | 2006-06-22 | Ge Healthcare Bio-Sciences Ab | Purification of immunoglobulins |
| US8728828B2 (en) * | 2004-12-22 | 2014-05-20 | Ge Healthcare Bio-Sciences Ab | Purification of immunoglobulins |
| US20080090995A1 (en) * | 2005-02-14 | 2008-04-17 | Ge Healthcare Bio-Sciences Ab | Liquid Chromatography Method |
| US8309709B2 (en) | 2005-07-06 | 2012-11-13 | Ge Healthcare Bio-Sciences Ab | Method of preparing a separation matrix |
| SE529259C2 (sv) * | 2005-08-31 | 2007-06-12 | Ge Healthcare Bio Sciences Ab | Tillverkning av kromarografimatriser, en kromarografimatris, en vätskekromatografikolonn, prcess för att isolera målföreningar och användning av en kromarografimatris för vätskekromatografi |
| SG131016A1 (en) * | 2005-09-19 | 2007-04-26 | Millipore Corp | Asymmetric porous adsorptive bead |
| US7608187B2 (en) * | 2005-11-21 | 2009-10-27 | Ge Healthcare Bio-Sciences Ab | Manufacture of a chromatography matrix |
| US20070161785A1 (en) * | 2005-12-05 | 2007-07-12 | Clontech Laboratories, Inc. | High density metal ion affinity compositions and methods for making and using the same |
| US8481298B2 (en) * | 2006-09-29 | 2013-07-09 | Ge Healthcare Bio-Sciences Ab | Separation matrix for viral purification |
| US9045566B2 (en) * | 2007-05-04 | 2015-06-02 | Bio-Works Technologies Ab | Method for the manufacture of agarose gels |
| JP5508850B2 (ja) | 2007-05-30 | 2014-06-04 | 株式会社カネカ | ホルミル基含有多孔質担体、それを用いた吸着体、およびそれらの製造方法 |
| SG149759A1 (en) | 2007-07-10 | 2009-02-27 | Millipore Corp | Media for affinity chromatography |
| CN101765458B (zh) * | 2007-07-25 | 2014-05-28 | 通用电气健康护理生物科学股份公司 | 分离基质 |
| US8664152B2 (en) | 2007-08-31 | 2014-03-04 | Jnc Corporation | Porous cellulose gel, method for producing the same and use thereof |
| WO2009099375A1 (en) * | 2008-02-05 | 2009-08-13 | Ge Healthcare Bio-Sciences Ab | Method for production of separation media |
| JP5826180B2 (ja) * | 2009-10-12 | 2015-12-02 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 分離マトリックス |
| WO2012081616A1 (ja) | 2010-12-14 | 2012-06-21 | Jsr株式会社 | 高分子粒子の製造方法、及び、高分子粒子 |
| EP2831096B1 (en) | 2012-03-28 | 2020-04-29 | GE Healthcare BioProcess R&D AB | Affinity chromatography matrix |
| IN2015DN03308A (index.php) | 2012-11-13 | 2015-10-09 | Ge Healthcare Bio Sciences Ab | |
| JP6348507B2 (ja) | 2012-12-14 | 2018-06-27 | ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ | 充填ベッドクロマトグラフィーカラムの洗浄方法 |
| CA2892628C (en) * | 2012-12-28 | 2021-08-10 | Nalco Company | Chemical treatment to improve red mud separation and washing in the bayer process |
| WO2014157606A1 (ja) * | 2013-03-29 | 2014-10-02 | 国立大学法人京都大学 | 架橋された疎水化多糖ナノゲル粒子とその製造方法 |
| WO2015046473A1 (ja) | 2013-09-27 | 2015-04-02 | 株式会社カネカ | アルカリ水溶液を用いた多孔質セルロースビーズの製造方法、リガンド固定化用担体および吸着体 |
| RU2665442C2 (ru) * | 2013-10-10 | 2018-08-29 | ДжиИ Хелткер Биопроцесс АрЭндДи АБ | Способ получения хроматографического материала |
| JP5883068B2 (ja) * | 2014-05-19 | 2016-03-09 | 株式会社カネカ | 多孔質担体、およびそれを用いた精製用吸着体、およびそれらの製造方法、およびそれらを用いた精製方法 |
| CN119751653A (zh) | 2014-06-24 | 2025-04-04 | 思拓凡生物工艺研发有限公司 | 层析方法 |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| CN106140038B (zh) * | 2015-04-08 | 2019-07-12 | 通用电气公司 | 制备聚合物微粒的方法和装置 |
| GB201509677D0 (en) * | 2015-06-04 | 2015-07-22 | Ge Healthcare Bio Sciences Ab | Manufacturing process for polysaccharide beads |
| GB201515339D0 (en) | 2015-08-28 | 2015-10-14 | Ge Healthcare Bio Sciences Ab | A seperation matrix and a method of seperating antibodies |
| US10427950B2 (en) | 2015-12-04 | 2019-10-01 | Ecolab Usa Inc. | Recovery of mining processing product using boronic acid-containing polymers |
| CN108883394B (zh) | 2016-04-06 | 2022-05-10 | 思拓凡生物工艺研发有限公司 | 色谱基质 |
| US10703774B2 (en) * | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| US10730908B2 (en) * | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| JP7106187B2 (ja) | 2016-05-11 | 2022-07-26 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックスを保存する方法 |
| US10654887B2 (en) * | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| EP3455243B1 (en) | 2016-05-11 | 2021-03-24 | Cytiva BioProcess R&D AB | Separation matrix |
| EP3455241B1 (en) | 2016-05-11 | 2022-02-23 | Cytiva BioProcess R&D AB | Method of cleaning and/or sanitizing a separation matrix |
| CN107537412B (zh) | 2016-06-27 | 2021-06-01 | 思拓凡生物工艺研发有限公司 | 制备聚合物微球的方法和系统 |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| GB201703116D0 (en) | 2017-02-27 | 2017-04-12 | Ge Healthcare Bioprocess R&D Ab | A seperation matrix and a method of seperating antibodies |
| GB201708277D0 (en) | 2017-05-24 | 2017-07-05 | Ge Healthcare | A Recombinant protein |
| GB201710130D0 (en) | 2017-06-26 | 2017-08-09 | Ge Healthcare Bioprocess R & D Ab | A Method of seperation |
| WO2019059400A1 (ja) | 2017-09-25 | 2019-03-28 | Jsr株式会社 | イムノグロブリン結合タンパク質、及びそれを用いたアフィニティー担体 |
| TWI888342B (zh) | 2017-09-25 | 2025-07-01 | 日商Ｊｓｒ股份有限公司 | 免疫球蛋白結合蛋白質，及使用其之親和性載體 |
| GB2573133A (en) | 2018-04-25 | 2019-10-30 | Ge Healthcare Bioprocess R&D Ab | Separation matrix and method of separation |
| GB201810690D0 (en) | 2018-06-29 | 2018-08-15 | Ge Healthcare Bioprocess R&D Ab | Chromatography beads, production and use thereof |
| EP3842536A4 (en) | 2018-08-24 | 2023-01-18 | JSR Corporation | IMMUNOGLOBULIN-BINDING PROTEIN AND AFFINITY CARRIER WITH IT |
| GB201820806D0 (en) | 2018-12-20 | 2019-02-06 | Ge Healthcare Bioprocess R&D Ab | Large pore agarose |
| EP3962923B1 (en) | 2019-04-29 | 2023-04-19 | Cytiva BioProcess R&D AB | Method for separation of antibodies or antibody fragments being devoid of an fc region capable of binding to protein a |
| GB201905919D0 (en) | 2019-04-29 | 2019-06-12 | Ge Healthcare Bioprocess R & D Ab | Method of manufacturing agar or agarose beads |
| GB202018588D0 (en) | 2020-11-26 | 2021-01-13 | Cytiva Bioprocess R & D Ab | Separation matrix |
| WO2022174005A1 (en) | 2021-02-12 | 2022-08-18 | Ecolab Usa Inc. | Purification of bauxite ores using boronic acid-functional compounds |
| GB202109246D0 (en) | 2021-06-28 | 2021-08-11 | Cytiva Bioprocess R & D Ab | A method of separating bispecific antibodies |
| GB202110014D0 (en) | 2021-07-12 | 2021-08-25 | Cytiva Bioprocess R & D Ab | A method for separating adeno-associated virus capsids, compositions obtained by said method and uses thereof |
| SE545486C2 (en) | 2021-07-15 | 2023-09-26 | Bio Works Tech Ab | Method for the manufacture of agar or agarose beads using vegetable oil |
| GB202113626D0 (en) | 2021-09-24 | 2021-11-10 | Cytiva Bioprocess R & D Ab | FC binding polypeptides |
| KR20240120721A (ko) | 2021-12-07 | 2024-08-07 | 씨티바 바이오프로세스 알＆디 에이비 | 분리 매트릭스 및 표적 분자를 분리하는 방법 |
| GB202202777D0 (en) | 2022-03-01 | 2022-04-13 | Cytiva Bioprocess R & D Ab | A method for separating supercoiled plasmid DNA |
| GB202203478D0 (en) | 2022-03-14 | 2022-04-27 | Cytiva Bioprocess R & D Ab | Vh3 binding polypeptides |
| GB202203640D0 (en) | 2022-03-16 | 2022-04-27 | Cytiva Bioprocess R & D Ab | Alkali-stabilized kappa light chain-binding separation matrix |
| GB202206123D0 (en) | 2022-04-27 | 2022-06-08 | Cytiva Bioprocess R & D Ab | A pre-screening method and a method for separating adeno-associated virus capsids |
| GB202214280D0 (en) | 2022-09-29 | 2022-11-16 | Cytiva Vioprocess R&D Ab | Chromatography ligand and chromatography material, and uses thereof |
| GB202215814D0 (en) | 2022-10-26 | 2022-12-07 | Cytiva Bioprocess R & D Ab | A chromatography device, system, and use thereof for analytic separation |
| WO2024217979A1 (en) | 2023-04-19 | 2024-10-24 | Cytiva Bioprocess R&D Ab | A method for purification of extracellular vesicles or enveloped viruses by means of size exclusion chromatography |
| CN121511131A (zh) | 2023-06-13 | 2026-02-10 | 思拓凡生物工艺研发有限公司 | 包膜或膜性生物颗粒的纯化 |
| US12312447B2 (en) | 2023-06-19 | 2025-05-27 | Zhejiang Triple Win New Material Co., Ltd. | Preparation method for polysaccharide spherical gel resin |
| WO2025036605A1 (en) | 2023-08-14 | 2025-02-20 | Cytiva Bioprocess R&D Ab | Vh3 binding separation matrix and methods for using the same |
| US20250073676A1 (en) | 2023-09-01 | 2025-03-06 | Purolite Llc | Separation matrices and methods for liquid chromatography with improved linear flow velocity |
| WO2025056387A1 (en) | 2023-09-12 | 2025-03-20 | Cytiva Bioprocess R&D Ab | A support material for solid-phase synthesis of oligonucleotides and peptides |
| KR102741170B1 (ko) * | 2023-10-13 | 2024-12-10 | 퓨리오젠 주식회사 | 다공성 아가 또는 아가로스 비드 및 이의 제조방법 |
| EP4541814A1 (en) | 2023-10-20 | 2025-04-23 | Cytiva BioProcess R&D AB | Stabilized polypeptides |
| WO2025083267A1 (en) | 2023-10-20 | 2025-04-24 | Cytiva Bioprocess R&D Ab | Aav9 binding polypeptides |
| EP4541444A1 (en) | 2023-10-20 | 2025-04-23 | Cytiva BioProcess R&D AB | Fusion protein for affinity capture |
| WO2025186218A1 (en) | 2024-03-04 | 2025-09-12 | Cytiva Bioprocess R&D Ab | Purification and analysis of rna |
| WO2025202371A1 (en) | 2024-03-27 | 2025-10-02 | Cytiva Bioprocess R&D Ab | Preparation of circular rna |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2247943A1 (de) * | 1971-10-01 | 1973-04-26 | Orion Yhtymae Oy | Verfahren zum vernetzen von agarose oder agar |
| US3873514A (en) | 1974-05-17 | 1975-03-25 | Bio Rad Laboratories | Preparation of gel for affinity chromatography |
| SE449225B (sv) * | 1980-12-18 | 1987-04-13 | Gelinnovation Hb | Gelprodukt baserad pa partiklar innehallande galaktan for separationsendamal, molekyl- och partikelimmobilisering |
| SE8205908D0 (sv) * | 1982-10-18 | 1982-10-18 | Pharmacia Diagnostics Ab | Sett att bilda polymerskikt |
| SE437270B (sv) * | 1983-07-19 | 1985-02-18 | Pharmacia Ab | Separationsmaterial av tverbunden agaros och dess framstellning separationsmaterial av tverbunden agaros och dess framstellning |
| US4582865A (en) * | 1984-12-06 | 1986-04-15 | Biomatrix, Inc. | Cross-linked gels of hyaluronic acid and products containing such gels |
| SE458525B (sv) * | 1985-05-23 | 1989-04-10 | Pharmacia Ab | Foerfarande foer tvaerbindning av en poroes agar- eller agarosgel |
| CA1329800C (en) * | 1987-12-29 | 1994-05-24 | Hiroaki Takayanagi | Composite separating agent |
| WO1993009176A2 (en) * | 1991-10-29 | 1993-05-13 | Clover Consolidated, Limited | Crosslinkable polysaccharides, polycations and lipids useful for encapsulation and drug release |
| SE9200827D0 (sv) * | 1992-03-18 | 1992-03-18 | Pharmacia Lkb Biotech | Supet porous polysaccharide gels |
| US5371208A (en) * | 1992-12-30 | 1994-12-06 | Guest Elchrom Scientific Ltd. | Preparation of cross-linked linear polysaccharide polymers as gels for electrophoresis |
| US5785832A (en) * | 1995-07-26 | 1998-07-28 | Chiari; Marcella | Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography |
| US5840877A (en) * | 1996-09-05 | 1998-11-24 | Guest Elchrom Scientific | Electrophoresis gels of enhanced selectivity |
| SE9700769D0 (sv) * | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matriser för separation och separation som utnyttjar matriserna |
-
1996
- 1996-04-11 SE SE9601368A patent/SE9601368D0/xx unknown
-
1997
- 1997-04-07 WO PCT/SE1997/000578 patent/WO1997038018A1/en not_active Ceased
- 1997-04-07 EP EP97919836A patent/EP0892815B1/en not_active Expired - Lifetime
- 1997-04-07 US US09/155,995 patent/US6602990B1/en not_active Expired - Lifetime
- 1997-04-07 ES ES97919836T patent/ES2217413T3/es not_active Expired - Lifetime
- 1997-04-07 DK DK97919836T patent/DK0892815T3/da active
- 1997-04-07 AU AU24178/97A patent/AU726086B2/en not_active Expired
- 1997-04-07 AT AT97919836T patent/ATE261992T1/de active
- 1997-04-07 JP JP53612197A patent/JP4081143B2/ja not_active Expired - Fee Related
- 1997-04-07 DE DE69728141T patent/DE69728141T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ATE261992T1 (de) | 2004-04-15 |
| AU726086B2 (en) | 2000-11-02 |
| ES2217413T3 (es) | 2004-11-01 |
| JP2000508361A (ja) | 2000-07-04 |
| EP0892815A1 (en) | 1999-01-27 |
| WO1997038018A1 (en) | 1997-10-16 |
| AU2417897A (en) | 1997-10-29 |
| DE69728141D1 (de) | 2004-04-22 |
| EP0892815B1 (en) | 2004-03-17 |
| US6602990B1 (en) | 2003-08-05 |
| DK0892815T3 (da) | 2004-06-21 |
| SE9601368D0 (sv) | 1996-04-11 |
| JP4081143B2 (ja) | 2008-04-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69728141T2 (de) | Verfahren zur herstellung eines porösen vernetzten polysaccharidgels und seine verwendung als filtermedien und in der chromatographie | |
| DE60015137T2 (de) | Mit material gefülllte geschäumte substanz | |
| DE69325266T2 (de) | Superporöse polysaccharidgele | |
| DE69830999T2 (de) | Verfahren zur adsorption/trennung | |
| DE3689327T2 (de) | Makroporöse Partikel. | |
| EP0521970B1 (de) | Wasserunlösliche cyclodextrin-polymerisate und verfahren zu deren herstellung | |
| DE2728146C2 (de) | Makroporöses Mischpolymerisat für die Flüssigkeitschromatographie und Verfahren zu seiner Herstellung | |
| EP0524969B1 (de) | Cyclodextrin-polymerisate und verfahren zu deren herstellung | |
| EP0154246B1 (de) | Phasenträger für die Verteilungschromatographie von Makromolekülen, Verfahren zu ihrer Herstellung und ihre Verwendung | |
| EP2274080B1 (de) | Hydrophobe cellulose-membran, verfahren zu ihrer herstellung und ihre verwendung in der hydrophoben interaktionschromatography | |
| EP3347125B1 (de) | Adsorptionsmedium, verfahren zu dessen herstellung, sowie verwendung desselben zur aufreinigung von biomolekülen | |
| DE69834558T2 (de) | Trennmittel für optische isomere und verfahren zu ihrer herstellung | |
| CH439756A (de) | Verfahren zur Herstellung von hydrophilen Polymeräthern in Form von Gelkörnern | |
| DE2005408B2 (de) | Verfahren zur Herstellung von Ionenaustauschern und danach hergestellte Ionenaustauscher | |
| DE69011650T2 (de) | Anionaustauscher. | |
| DE69222904T2 (de) | Neue polysaccharidverbindung und trennmittel | |
| DE2005407C2 (de) | Verfahren zur Herstellung von Ionenaustauschern auf Cellulosebasis | |
| DE69905590T2 (de) | Verfahren zur herstellung von polysaccharideperlen | |
| DE60212619T2 (de) | Herstellungsverfahren eines füllers zur trennung eines optischen isomers und anwendung in der chromatographie | |
| DE69027439T2 (de) | Vernetztes copolymerteilchen und verfahren zu dessen herstellung | |
| DE60131710T2 (de) | Verfahren zur Adsorption und Entfernung, und Verwendung eines Adsorbers für endogene Cannabinoide | |
| DE1443396C3 (de) | Verfahren zur Herstellung von hochmolekularen, ionenaustauschende Gruppen enthaltenden, hydrophilen Vernetzungsprodukten | |
| DE2819522C2 (index.php) | ||
| DE3900272A1 (de) | Synthetisches polymeres traegermaterial fuer chromatographische trennverfahren, verfahren zu seiner herstellung und verwendung | |
| DE4127657A1 (de) | Perlfoermige polyvinylalkoholgele fuer die aufreinigung und auftrennung biologischer fluessigkeiten, verfahren zu ihrer herstellung und verwendung |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8328 | Change in the person/name/address of the agent |
Representative=s name: HAMMONDS RECHTSANWAELTE PATENTANWAELTE, 80539 MUENCHEN |
|
| 8327 | Change in the person/name/address of the patent owner |
Owner name: GE HEALTHCARE BIO-SCIENCES AB, UPPSALA, SE |
|
| 8328 | Change in the person/name/address of the agent |
Representative=s name: HAMMONDS LLP, LONDON, GB |
|
| R082 | Change of representative |
Ref document number: 892815 Country of ref document: EP Representative=s name: J D REYNOLDS & CO., GB |