DE602008005746D1 - Vorrichtung, system und verfahren zur beobachtung und/oder kultivierung mikroskopischer objekte - Google Patents

Vorrichtung, system und verfahren zur beobachtung und/oder kultivierung mikroskopischer objekte

Info

Publication number
DE602008005746D1
DE602008005746D1 DE602008005746T DE602008005746T DE602008005746D1 DE 602008005746 D1 DE602008005746 D1 DE 602008005746D1 DE 602008005746 T DE602008005746 T DE 602008005746T DE 602008005746 T DE602008005746 T DE 602008005746T DE 602008005746 D1 DE602008005746 D1 DE 602008005746D1
Authority
DE
Germany
Prior art keywords
objects
cultivation
microscopic objects
microscopic
monitoring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602008005746T
Other languages
English (en)
Inventor
Niels B Ramsing
Joergen Berntsen
Jens K Gundersen
Holger Soee Plougsgaard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unisense Fertilitech AS
Original Assignee
Unisense Fertilitech AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unisense Fertilitech AS filed Critical Unisense Fertilitech AS
Publication of DE602008005746D1 publication Critical patent/DE602008005746D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
DE602008005746T 2007-06-29 2008-06-27 Vorrichtung, system und verfahren zur beobachtung und/oder kultivierung mikroskopischer objekte Active DE602008005746D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200700952 2007-06-29
PCT/DK2008/050162 WO2009003487A2 (en) 2007-06-29 2008-06-27 A device, a system and a method for monitoring and/or culturing of microscopic objects

Publications (1)

Publication Number Publication Date
DE602008005746D1 true DE602008005746D1 (de) 2011-05-05

Family

ID=40226567

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602008005746T Active DE602008005746D1 (de) 2007-06-29 2008-06-27 Vorrichtung, system und verfahren zur beobachtung und/oder kultivierung mikroskopischer objekte

Country Status (9)

Country Link
US (2) US8633017B2 (de)
EP (1) EP2173853B1 (de)
JP (1) JP5143227B2 (de)
CN (2) CN103642688B (de)
AT (1) ATE503000T1 (de)
DE (1) DE602008005746D1 (de)
DK (1) DK2173853T3 (de)
ES (1) ES2363406T3 (de)
WO (1) WO2009003487A2 (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5451073B2 (ja) 2005-10-14 2014-03-26 ユニセンス・ファーティリテック・アクティーゼルスカブ 細胞集団における変化の決定
ES2363406T3 (es) 2007-06-29 2011-08-03 Unisense Fertilitech A/S Dispositivo, sistema y método para monitorizar y/o cultivar objetos microscópicos.
US20100075411A1 (en) * 2008-09-22 2010-03-25 Cecchi Michael D Specimen manipulation device for micro manipulation and biopsy in assisted reproduction and in vitro fertilization
JP2010220511A (ja) * 2009-03-23 2010-10-07 Nikon Corp 培養観察装置及び情報管理方法
CN104293646A (zh) 2009-08-22 2015-01-21 里兰斯坦福初级大学理事会 成像并评估胚胎、卵母细胞和干细胞
CA2812776C (en) * 2010-09-27 2022-05-17 Auxogyn, Inc. Apparatus, method, and system for the automated imaging and evaluation of embryos, oocytes, and stem cells
WO2012116185A1 (en) 2011-02-23 2012-08-30 The Board Of Trustees Of The Leland Stanford Junior University Methods of detecting aneuploidy in human embryos
US20140128667A1 (en) * 2011-07-02 2014-05-08 Unisense Fertilitech A/S Adaptive embryo selection criteria optimized through iterative customization and collaboration
TWI498559B (zh) * 2012-05-04 2015-09-01 Univ Nat Taiwan Ocean 細胞代謝率檢測系統及其細胞代謝率檢測微型結構
JP6278590B2 (ja) * 2012-11-02 2018-02-14 大日本印刷株式会社 細胞培養容器および細胞培養方法
ES2755878T3 (es) 2013-02-01 2020-04-24 Ares Trading Sa Fenotipos anormales de singamia observados con obtención de imágenes a intervalos de tiempo preestablecidos para identificación precoz de embriones con potencial de desarrollo inferior
EP2781591B1 (de) * 2013-03-19 2017-05-03 Unisense Fertilitech A/S Schale, System und Verfahren zur Überwachung und Kultivierung einer Zellkultur
JPWO2014196204A1 (ja) * 2013-06-07 2017-02-23 株式会社クラレ 培養容器及び培養方法
CN109337804B (zh) * 2013-07-05 2022-07-08 爱特医疗有限公司 一种用于监测生物材料发育的设备
WO2015001397A1 (en) 2013-07-05 2015-01-08 At Medical Ltd. A device for culturing a biological material
JP5920296B2 (ja) * 2013-09-03 2016-05-18 大日本印刷株式会社 細胞培養容器
CN104830681A (zh) * 2014-02-09 2015-08-12 李保伟 一种细胞培养载玻片系统
JP6364817B2 (ja) * 2014-02-28 2018-08-01 大日本印刷株式会社 細胞培養容器
US10942170B2 (en) 2014-03-20 2021-03-09 Ares Trading S.A. Quantitative measurement of human blastocyst and morula morphology developmental kinetics
GB2525884A (en) * 2014-05-07 2015-11-11 Unisense Fertilitech As Culture dish
SG11201609916RA (en) * 2014-05-30 2016-12-29 Kuraray Co Culture method and cell mass
JP2016049046A (ja) * 2014-08-29 2016-04-11 大日本印刷株式会社 画像管理装置及びプログラム
JP6452401B2 (ja) * 2014-11-20 2019-01-16 日機装株式会社 透析液供給システム
GB2539935A (en) 2015-07-01 2017-01-04 Insphero Ag Device for propagating microtissues
US11494578B1 (en) * 2015-09-21 2022-11-08 Ares Trading S.A. Systems and methods for automated assessment of embryo quality using image based features
US10510143B1 (en) * 2015-09-21 2019-12-17 Ares Trading S.A. Systems and methods for generating a mask for automated assessment of embryo quality
CN108779423B (zh) 2016-03-28 2021-12-21 富士胶片株式会社 Pcr用容器
ES2807194T3 (es) 2016-06-30 2021-02-22 Esco Medical Aps Un aparato para la incubación de un material biológico
DK179163B9 (en) * 2016-07-01 2018-04-03 Esco Medical Uab An apparatus for the combined incubation and vitrification of a biological material
US20190225923A1 (en) * 2016-09-30 2019-07-25 Nanyang Technological University Apparatus for embryo biopsy
CN107749936A (zh) * 2017-10-12 2018-03-02 武汉互创联合科技有限公司 一种用于胚胎、卵母细胞或干细胞的成像系统
JP6732722B2 (ja) * 2017-12-11 2020-08-05 憲隆 福永 胚選抜システム
US20190382701A1 (en) * 2018-06-18 2019-12-19 SageMedic Corporation System for Obtaining 3D Micro-Tissues
CN109628275A (zh) * 2019-01-11 2019-04-16 上海皓信生物科技有限公司 丝状真菌的改良小培养装置及其培养方法
US20220275328A1 (en) * 2020-06-25 2022-09-01 Next & Bio Inc. Method for mass proliferation of stem cells without using hydrogel
WO2021261622A1 (ko) * 2020-06-25 2021-12-30 주식회사 넥스트앤바이오 표준형 오가노이드 제조방법
JP2023538206A (ja) * 2020-06-25 2023-09-07 ネクストアンドバイオ インコーポレイテッド ハイドロゲルを用いない人工多能性幹細胞の製造方法
WO2021261623A1 (ko) * 2020-06-25 2021-12-30 주식회사 넥스트앤바이오 뇌 오가노이드 제작방법
FR3136480A1 (fr) * 2022-06-13 2023-12-15 4Dcell Dispositif de culture cellulaire

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3618734A (en) 1969-06-10 1971-11-09 Res Foundation Of Children S H Specimen incubator
GB1426786A (en) 1973-09-12 1976-03-03 British Petroleum Co Microbiological method for estimating the toxicity of liquid industrial effluents
DE2940446C2 (de) 1979-10-05 1982-07-08 B. Braun Melsungen Ag, 3508 Melsungen Züchtung von tierischen Zellen in Suspensions- und Monolayerkulturen in Fermentationsgefäßen
US4724543A (en) 1985-09-10 1988-02-09 Beckman Research Institute, City Of Hope Method and apparatus for automatic digital image analysis
NL8600396A (nl) 1986-02-17 1987-09-16 Rijkslandbouwhogeschool Werkwijze voor het bepalen van de respiratiesnelheid van een respirerend materiaal in een continue processtroom alsmede een voor een dergelijke toepassing geschikte inrichting.
JP2662215B2 (ja) * 1986-11-19 1997-10-08 株式会社日立製作所 細胞保持装置
JP2559760B2 (ja) * 1987-08-31 1996-12-04 株式会社日立製作所 細胞搬送方法
JP2564322B2 (ja) 1987-10-07 1996-12-18 株式会社日立製作所 細胞融合装置
DE3836716A1 (de) 1988-10-28 1990-05-03 Zeiss Carl Fa Verfahren zur auswertung von zellbildern
CA2072115A1 (en) 1989-10-30 1991-05-01 Dallas E. Hughes Expression of bacterial hemoglobin and enhancement of expression of cloned and native products in streptomyces
ATE132537T1 (de) 1990-03-29 1996-01-15 Avl Photronics Corp Verfahren und apparat zum nachweis biologischer aktivitäten in einer probe
AU2016392A (en) 1991-05-14 1992-12-30 Mount Sinai School Of Medicine Of The City University Of New York, The Method and apparatus for (in vitro) fertilization
US5196168A (en) 1991-12-19 1993-03-23 Eastman Kodak Company Incubator with positioning device for slide elements
AU658079B2 (en) 1992-09-28 1995-03-30 Becton Dickinson & Company Cell culture insert
US5763279A (en) 1993-09-09 1998-06-09 Synthecon, Inc. Gas permeable bioreactor and method of use
EP0718405B1 (de) 1994-12-20 2002-10-02 Cosmo Research Institute 5-Aminolävulinsäure produzierender Mikroorganismus
US6030828A (en) 1995-11-22 2000-02-29 Unisense Aps. Microsensor and use of such microsensor
EP0853657B1 (de) 1996-08-05 2007-12-26 Thermo Electron LED GmbH Objekt-lagervorrichtung, lagerstation und klimaschrank
DK172855B1 (da) 1996-11-12 1999-08-16 Unisense Aps Fremgangsmåde til måling af koncentrationen af et medium i et miljø, mikrosensor til brug ved fremgangsmåden og anvendelse
US6306491B1 (en) 1996-12-20 2001-10-23 Gore Enterprise Holdings, Inc. Respiratory aids
US5968340A (en) 1997-04-07 1999-10-19 Marine Biological Laboratory Polarographic self-referencing probe and method for using
US6228636B1 (en) 1998-09-21 2001-05-08 Matsushita Electric Industrial Co., Ltd. Incubator
DE19903506C2 (de) 1999-01-29 2002-04-04 Inst Chemo Biosensorik Verfahren, Gefäß und Vorrichtung zur Überwachung der Stoffwechselaktivität von Zellkulturen in flüssigen Medien
US6391577B1 (en) 1999-03-03 2002-05-21 Susan R. Mikkelsen Rapid electrochemical assay for antibiotic and cytotoxic drug susceptibility in microorganisms
EP1214593A2 (de) 1999-06-30 2002-06-19 Knoll GmbH Verfahren zur identifizierung von verbindungen, die amp-empfindliche regulatorische stellen in mitochondrien modulieren
AU5061300A (en) * 1999-06-30 2001-01-22 Danish Institute of Agricultural Sciences, The Microchamber for embryo cell culture
US6998249B1 (en) 1999-09-27 2006-02-14 Pharmacia & Upjohn Company Toxicity screening method
US6395555B1 (en) 1999-10-14 2002-05-28 David F. Wilson Method and apparatus for determining the effect of a drug on cells
RU2187111C2 (ru) 1999-11-02 2002-08-10 Кубанский государственный университет Способ определения функционального состояния фолликулов млекопитающих
EP1134583A1 (de) 2000-03-17 2001-09-19 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO Messung von Änderungen in der Stoffwechselaktivität
DE10017192A1 (de) 2000-04-07 2001-10-11 Kendro Lab Prod Gmbh Brutschrank sowie Verfahren zur Entriegelung und Verwendung
JP2001330582A (ja) 2000-05-24 2001-11-30 Japan Science & Technology Corp 細胞呼吸活性評価方法及びその装置
JP3442357B2 (ja) * 2000-08-25 2003-09-02 株式会社日立製作所 両生類卵母細胞試料導入装置、両生類卵母細胞試料導入システム、両生類卵母細胞試料導入方法、両生類卵母細胞の製造方法、両生類卵母細胞及びそれを販売又は譲渡する方法、スクリーニング用のセンサーとして用いる方法、容器、及び解析方法
JP3693907B2 (ja) 2000-10-13 2005-09-14 独立行政法人科学技術振興機構 哺乳動物胚の無侵襲的品質評価方法及びその装置
US6434320B1 (en) 2000-10-13 2002-08-13 Comtrak Technologies, Llc Method of searching recorded digital video for areas of activity
WO2002045018A1 (fr) 2000-12-01 2002-06-06 Japan Science And Technology Corporation Procede de determination des zones nucleaires et procede d'etablissement de genealogie nucleaire
US6555365B2 (en) 2000-12-07 2003-04-29 Biocrystal, Ltd. Microincubator comprising a cell culture apparatus and a transparent heater
JP3738899B2 (ja) * 2000-12-07 2006-01-25 株式会社 エフェクター細胞研究所 微量試料処理装置
FR2820756B1 (fr) 2001-02-09 2004-01-23 Daniel Attias Incubateur et procede d'incubation menageant l'organisme mis a incuber
JP2002262856A (ja) 2001-03-07 2002-09-17 Japan Tissue Engineering:Kk 自動培地交換方法、プログラム及び自動培地交換装置
EP2420824B1 (de) * 2001-06-29 2018-11-28 Meso Scale Technologies LLC Multiwell-Platte mit einer Reihe von Wells und Kit für den Einsatz bei der Durchführung eines ECL-Assays
US7015031B2 (en) 2002-01-24 2006-03-21 Genx International, Inc. Biological specimen-culturing system and method with onboard specimen development sensors
JP4287287B2 (ja) 2002-02-13 2009-07-01 レイファイ コーポレイション 空間時間信号の取得、圧縮、及び特徴づけのための方法及び装置
JP2004166554A (ja) 2002-11-19 2004-06-17 Sanyo Electric Co Ltd インキュベータ
TWI249574B (en) 2002-11-19 2006-02-21 Sanyo Electric Co Preserving device
JP4800032B2 (ja) 2002-12-23 2011-10-26 ユニセンス・レスピロメトリー・アクティーゼルスカブ 実質的に球形の代謝粒子の個々の代謝速度の非−侵入的測定のためのデバイスおよび方法
FR2850997B1 (fr) 2003-02-10 2006-01-13 Jean Lermite Piscine modulaire
US20060147935A1 (en) * 2003-02-12 2006-07-06 Sten Linnarsson Methods and means for nucleic acid sequencing
US20060201883A1 (en) * 2003-02-19 2006-09-14 Hofmeister William H Polymer matrixes having nanoscale channels and uses
US7372985B2 (en) 2003-08-15 2008-05-13 Massachusetts Institute Of Technology Systems and methods for volumetric tissue scanning microscopy
US7239719B2 (en) 2003-08-22 2007-07-03 Bbn Technologies Corp. Automatic target detection and motion analysis from image data
US20050118563A1 (en) 2003-08-28 2005-06-02 Geoffrey Sher Method for determining embryo quality
US20070015289A1 (en) * 2003-09-19 2007-01-18 Kao H P Dispenser array spotting
JP4454998B2 (ja) 2003-09-29 2010-04-21 株式会社ニコン 細胞観察装置および細胞観察方法
WO2005039181A1 (ja) 2003-10-21 2005-04-28 Matsushita Electric Industrial Co., Ltd. 監視装置
JP4439882B2 (ja) 2003-11-14 2010-03-24 キヤノン株式会社 放射線画像処理装置及び処理方法
JP3950972B2 (ja) 2003-11-14 2007-08-01 国立大学法人名古屋大学 生物試料の生物発光測定装置
JP2005168341A (ja) 2003-12-09 2005-06-30 Olympus Corp 生体試料観察装置
GB2409029A (en) 2003-12-11 2005-06-15 Sony Uk Ltd Face detection
US7336401B2 (en) 2003-12-19 2008-02-26 Xerox Corporation Systems and methods for estimating an image marking process using event mapping of scanned image attributes
EP1702052A4 (de) * 2003-12-19 2009-02-18 Univ Waterloo Kultivierte zelle sowie verfahren und vorrichtung zur zellkultur
US7187286B2 (en) 2004-03-19 2007-03-06 Applera Corporation Methods and systems for using RFID in biological field
JP4504089B2 (ja) * 2004-05-10 2010-07-14 富士通株式会社 マイクロインジェクション装置およびマイクロインジェクション方法
JP4749684B2 (ja) 2004-07-02 2011-08-17 株式会社ジェイテック インキュベータ及びそれに用いる培養カセット
JP2006034256A (ja) 2004-07-30 2006-02-09 Sanyo Electric Co Ltd 保管装置
GB0505379D0 (en) * 2005-03-16 2005-04-20 Robio Systems Ltd Cellular entity maturation and transportation systems
JP4665589B2 (ja) 2005-04-01 2011-04-06 ニプロ株式会社 細胞培養用容器
US9376657B2 (en) 2005-07-05 2016-06-28 Nikon Corporation Culture device
JP5451073B2 (ja) 2005-10-14 2014-03-26 ユニセンス・ファーティリテック・アクティーゼルスカブ 細胞集団における変化の決定
US20070090166A1 (en) * 2005-10-18 2007-04-26 Shuichi Takayama Microfluidic cell culture device
GB0521884D0 (en) 2005-10-27 2005-12-07 Newcastle Upon Tyne Hospitals Laboratory apparatus
JP2007334141A (ja) 2006-06-16 2007-12-27 Sanyo Electric Co Ltd 培養物観察システム
EP2035548B1 (de) 2006-06-16 2010-08-11 Unisense Fertilitech A/S Embryonenqualitätsbeurteilung auf der grundlage von blastomerenteilung und bewegung
JP5841304B2 (ja) 2006-06-16 2016-01-13 パナソニックヘルスケアホールディングス株式会社 培養物観察システム
US20080056952A1 (en) * 2006-08-25 2008-03-06 Angros Lee H Analytic plates with markable portions and methods of use
ES2363406T3 (es) 2007-06-29 2011-08-03 Unisense Fertilitech A/S Dispositivo, sistema y método para monitorizar y/o cultivar objetos microscópicos.

Also Published As

Publication number Publication date
ES2363406T3 (es) 2011-08-03
US20140141463A1 (en) 2014-05-22
JP2010531644A (ja) 2010-09-30
US9588104B2 (en) 2017-03-07
CN103642688A (zh) 2014-03-19
CN101802166A (zh) 2010-08-11
CN103642688B (zh) 2018-05-01
DK2173853T3 (da) 2011-07-04
WO2009003487A3 (en) 2009-05-07
WO2009003487A2 (en) 2009-01-08
EP2173853B1 (de) 2011-03-23
EP2173853A2 (de) 2010-04-14
ATE503000T1 (de) 2011-04-15
JP5143227B2 (ja) 2013-02-13
CN101802166B (zh) 2013-12-11
US20110165609A1 (en) 2011-07-07
US8633017B2 (en) 2014-01-21

Similar Documents

Publication Publication Date Title
DE602008005746D1 (de) Vorrichtung, system und verfahren zur beobachtung und/oder kultivierung mikroskopischer objekte
Hendriks et al. Artificial gametes: a systematic review of biological progress towards clinical application
FR2943685B1 (fr) Reacteur photosynthetique pour la culture de microorganiques et procede de culture de microorganismes
WO2010056755A3 (en) Microfluidic embryo and gamete culture systems
BRPI0811177A2 (pt) Titulações de polipeptídeo do fator viii em culturas de células
ATE493203T1 (de) Verfahren und gerät zur herstellung von genetisch transformierbarem pflanzengewebe
CL2008003629A1 (es) Metodo de analisis microbiologico de muestras biologicas que comprende un primer intervalo de tiempo de incubacion, tras el cual las muestras que dividen en 3 categorias (positivas, negativas o inciertas) dependiendo de la cantidad de dioxido de carbono generado, y donde las muestra que presentan un contenido de dioxido de carbono comprendido entre un valor maximo y minimo (muestras inciertas), se someten a un segundo intervalo de tiempo de incubacion y asi categorizarlas como positivas o negativas.
EA025172B9 (ru) Способ оценки способности достижения одним или более эмбрионами человека стадии бластоцисты
FR2866655B1 (fr) Appareil et procede pour la preparation et la mise en culture de cellules
BR112012005844A2 (pt) Método para cultivar microalgas capazes de crescimento heterotrófico, método para fabricar um material, e sistema de biorreator
ATE494358T1 (de) Vorrichtung und verfahren zur fermentation in hohen zelldichten
WO2012170989A3 (en) Process of managed ecosystem fermentation
BR112013020217B1 (pt) método para cultivar de organismos em um fotobiorreator tubular
MY173319A (en) A method and a system for mass-cultivating microalgae with enhanced photosynthetic efficiency
EP4257141A3 (de) Stabilisiertes amorphes calciumcarbonat zur behandlung von neurologischen, muskel- und unfruchtbarkeitserkrankungen oder zuständen
Ahmad Latiffi et al. Experimental assessment on effects of growth rates microalgae Scenedesmus sp. in different conditions of pH, temperature, light intensity and photoperiod
CN102533930A (zh) 一种用于丝状真菌显微形态观察的制片方法
CA2837462C (en) Bioartificial proximal tubule systems and methods of use
BR112015015466A2 (pt) método para cultivar células-tronco pluripotentes
WO2010150013A3 (en) Identification of sex-linked proteins
Momeni-Moghaddam et al. A simple method for isolation, culture, and in vitro maintenance of chicken spermatogonial stem cells
GB2450599A (en) Automated culture of stem cells
EP3231865A3 (de) Verfahren zum einbringen eines polynukleotids in nicht-adhäsive pflanzenzellenzüchtung
CO2018003988A2 (es) Un método de cultivo de células
PH12016501509A1 (en) Air accordion bioreactor