DE602004006725D1 - Reinigung von Antikörpern durch Protein A und Ionenaustauschromatographie - Google Patents
Reinigung von Antikörpern durch Protein A und IonenaustauschromatographieInfo
- Publication number
- DE602004006725D1 DE602004006725D1 DE602004006725T DE602004006725T DE602004006725D1 DE 602004006725 D1 DE602004006725 D1 DE 602004006725D1 DE 602004006725 T DE602004006725 T DE 602004006725T DE 602004006725 T DE602004006725 T DE 602004006725T DE 602004006725 D1 DE602004006725 D1 DE 602004006725D1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- antibodies
- purification
- ion exchange
- exchange chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 238000004255 ion exchange chromatography Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
- C07K17/02—Peptides being immobilised on, or in, an organic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0304576.2A GB0304576D0 (en) | 2003-02-28 | 2003-02-28 | Protein a chromatography |
GB0304576 | 2003-02-28 | ||
PCT/EP2004/002041 WO2004076485A1 (en) | 2003-02-28 | 2004-03-01 | Antibody purification by protein a and ion exchange chromatography |
Publications (2)
Publication Number | Publication Date |
---|---|
DE602004006725D1 true DE602004006725D1 (de) | 2007-07-12 |
DE602004006725T2 DE602004006725T2 (de) | 2008-02-07 |
Family
ID=9953825
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602004006725T Expired - Lifetime DE602004006725T2 (de) | 2003-02-28 | 2004-03-01 | Reinigung von Antikörpern durch Protein A und Ionenaustauschromatographie |
Country Status (9)
Country | Link |
---|---|
US (3) | US20060030696A1 (de) |
JP (2) | JP2007525412A (de) |
KR (1) | KR101200732B1 (de) |
CN (1) | CN100384874C (de) |
AT (1) | ATE363491T1 (de) |
DE (1) | DE602004006725T2 (de) |
ES (1) | ES2288252T3 (de) |
GB (1) | GB0304576D0 (de) |
TW (1) | TWI325428B (de) |
Families Citing this family (60)
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US8658773B2 (en) | 2011-05-02 | 2014-02-25 | Immunomedics, Inc. | Ultrafiltration concentration of allotype selected antibodies for small-volume administration |
US20160279239A1 (en) | 2011-05-02 | 2016-09-29 | Immunomedics, Inc. | Subcutaneous administration of anti-cd74 antibody for systemic lupus erythematosus and autoimmune disease |
GB0220894D0 (en) * | 2002-09-09 | 2002-10-16 | Millipore Uk Ltd | Method of separation |
GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
WO2005077528A1 (en) * | 2004-02-05 | 2005-08-25 | Millipore Corporation | Porous adsorptive or chromatographic media |
BRPI0516572A (pt) * | 2004-10-05 | 2008-09-16 | Wyeth Corp | métodos e composições para melhorar a produção de proteìnas recombinantes |
US20160355591A1 (en) | 2011-05-02 | 2016-12-08 | Immunomedics, Inc. | Subcutaneous anti-hla-dr monoclonal antibody for treatment of hematologic malignancies |
RU2457214C2 (ru) * | 2005-03-11 | 2012-07-27 | Вайет | Способ хроматографии в режиме слабого распределения |
CN104926938B (zh) | 2005-06-17 | 2019-06-04 | 惠氏有限责任公司 | 纯化含Fc区蛋白的方法 |
ZA200900837B (en) * | 2006-08-28 | 2010-05-26 | Ares Trading Sa | Process for the purification of FC-containing proteins |
AU2008256411B2 (en) | 2007-06-01 | 2013-08-22 | F. Hoffmann-La Roche Ag | Immunoglobulin purification |
US9433922B2 (en) * | 2007-08-14 | 2016-09-06 | Emd Millipore Corporation | Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same |
US20090130738A1 (en) * | 2007-11-19 | 2009-05-21 | Mikhail Kozlov | Media for membrane ion exchange chromatography |
US20090286283A1 (en) * | 2008-05-16 | 2009-11-19 | Samsung Electronics Co., Ltd. | Method and affinity column for purifying proteins |
US8592555B2 (en) | 2008-08-11 | 2013-11-26 | Emd Millipore Corporation | Immunoglobulin-binding proteins with improved specificity |
EP2335076B1 (de) * | 2008-09-15 | 2012-08-08 | EMD Millipore Corporation | Verfahren zur quantifizierung des proteinverlusts von affinitätschromatographieharzen auf proteinbasis |
JP5626526B2 (ja) | 2008-09-25 | 2014-11-19 | Jsr株式会社 | アフィニティークロマトグラフィー用充填剤 |
US10419541B2 (en) | 2008-11-26 | 2019-09-17 | Free Stream Media Corp. | Remotely control devices over a network without authentication or registration |
EP2360183B1 (de) | 2008-12-19 | 2016-07-27 | Takeda Pharmaceutical Company Limited | Antikörperreinigungsverfahren mittels "mixed-mode" chromatography unter verwendung einer arginine-haltigen beladungslösung |
JP2012513425A (ja) | 2008-12-22 | 2012-06-14 | エフ.ホフマン−ラ ロシュ アーゲー | 免疫グロブリンの精製 |
SG162687A1 (en) * | 2008-12-24 | 2010-07-29 | Millipore Corp | Caustic stable chromatography ligands |
KR20190018041A (ko) | 2009-08-06 | 2019-02-20 | 제넨테크, 인크. | 단백질 정제 시의 바이러스 제거의 개선방법 |
IN2012DN00338A (de) * | 2009-08-07 | 2015-05-22 | Millipore Corp | |
EP2478005B1 (de) * | 2009-09-15 | 2014-11-12 | Ajinomoto Althea, Inc. | Protein-a-kristalle und vernetzte kristalle sowie verwendungsverfahren dafür |
AU2010310748C1 (en) | 2009-10-20 | 2015-11-26 | Abbvie Inc. | Isolation and purification of anti-IL-13 antibodies using Protein A affinity chromatography |
WO2011073389A1 (en) | 2009-12-18 | 2011-06-23 | Novartis Ag | Wash solution and method for affinity chromatography |
EP2519537A4 (de) * | 2009-12-29 | 2013-07-10 | Reddys Lab Ltd Dr | Reinigung von proteinen |
EP2583973B1 (de) * | 2010-06-21 | 2018-03-21 | Kyowa Hakko Kirin Co., Ltd. | Verfahren zur reinigung von proteinen mit aminosäuren |
CN103189390A (zh) * | 2010-11-01 | 2013-07-03 | 帝斯曼知识产权资产管理有限公司 | 单一单元离子交换色谱抗体纯化 |
MX357821B (es) * | 2010-12-21 | 2018-07-25 | Hoffmann La Roche | Preparación de anticuerpo enriquecida con isoforma y método para obtenerla. |
IL212911A0 (en) * | 2011-05-16 | 2011-07-31 | Omrix Biopharmaceuticals Ltd | Immunoglobulin reduced in thrombogenic contaminants and preparation thereof |
WO2012160536A1 (en) * | 2011-05-26 | 2012-11-29 | Dr Reddy's Laboratories Limited | Antibody purification |
SG10201604554WA (en) | 2011-06-08 | 2016-07-28 | Emd Millipore Corp | Chromatography matrices including novel staphylococcus aureus protein a based ligands |
WO2013009526A1 (en) * | 2011-07-08 | 2013-01-17 | Merck Sharp & Dohme Corp. | Method for purifying fc-fusion protein |
WO2013028330A2 (en) * | 2011-08-19 | 2013-02-28 | Emd Millipore Corporation | Methods of reducing level of one of more impurities in a sample during protein purification |
CN104066748A (zh) * | 2011-11-21 | 2014-09-24 | 霍夫曼-拉罗奇有限公司 | 抗c-met抗体的纯化 |
EP2791176B1 (de) * | 2011-12-15 | 2018-07-11 | Prestige Biopharma Pte. Ltd. | Verfahren zur antikörperreinigung |
EP2682168A1 (de) * | 2012-07-02 | 2014-01-08 | Millipore Corporation | Reinigung biologischer Moleküle |
CN103073616A (zh) * | 2013-01-06 | 2013-05-01 | 西北大学 | 使用混合模式层析技术去除抗体聚集体的方法 |
RU2646098C2 (ru) | 2013-04-30 | 2018-03-01 | Интас Фармасьютикалс Лтд | Новый способ клонирования, экспрессии и очистки для получения ранибизумаба |
EP3048109A4 (de) * | 2013-09-17 | 2017-04-19 | Kaneka Corporation | Neuartiges antikörperaufreinigungsverfahren und antikörper daraus sowie neuartiges antikörperaufreinigungsverfahren unter verwendung eines kationenaustauschers und antikörper daraus |
KR102123700B1 (ko) * | 2013-12-17 | 2020-06-16 | (주)셀트리온 | 혼합 방식 크로마토그래피를 이용한 침출된 단백질 a 제거 방법 |
JP6612242B2 (ja) * | 2014-02-14 | 2019-11-27 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 自動化された多段階精製システム及び多段階クロマトグラフィー精製方法 |
EP3118210B1 (de) * | 2014-03-11 | 2019-11-13 | Green Cross Holdings Corporation | Verfahren zur aufreinigung von immunglobulin |
ES2785375T3 (es) * | 2014-03-11 | 2020-10-06 | Green Cross Holdings Corp | Procedimiento de purificación de inmunoglobulina |
WO2015138548A1 (en) * | 2014-03-14 | 2015-09-17 | Merck Sharp & Dohme Corp. | Purifying insulin using cation exchange and reverse phase chromatography in the presence of an organic modifier and elevated temperature |
JP6451118B2 (ja) * | 2014-07-17 | 2019-01-16 | 東ソー株式会社 | 抗体の分離方法 |
KR101672947B1 (ko) | 2015-03-17 | 2016-11-04 | 주식회사 비에스엘 | 상황 알림 기능을 가진 히어링 디바이스 및 그에 의한 상황 알림 방법 |
US20160280767A1 (en) | 2015-03-23 | 2016-09-29 | Lonza Ltd. | Methods for controlling protein glycosylation |
AU2016288678B2 (en) | 2015-06-30 | 2021-05-27 | Vapotherm, Inc. | Nasal cannula for continuous and simultaneous delivery of aerosolized medicament and high flow therapy |
US11186858B1 (en) | 2016-03-15 | 2021-11-30 | Fresenius Kabi Deutschland Gmbh | Methods for increasing biosimilarity |
US10688412B2 (en) | 2016-07-25 | 2020-06-23 | Cehpalon, Inc. | Affinity chromatography wash buffer |
CN106380519B (zh) * | 2016-10-17 | 2019-11-01 | 深圳万乐药业有限公司 | 一种单克隆抗体的纯化方法 |
WO2018187074A1 (en) | 2017-04-03 | 2018-10-11 | Immunomedics, Inc. | Subcutaneous administration of antibody-drug conjugates for cancer therapy |
US11724056B2 (en) | 2017-09-08 | 2023-08-15 | Vapotherm, Inc. | Birfurcated cannula device |
CN111902720A (zh) | 2018-03-21 | 2020-11-06 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
EP3816177A4 (de) * | 2018-06-29 | 2021-12-01 | Mitsubishi Chemical Corporation | Verfahren zur polypeptidisolierung, verfahren zur polypeptidherstellung und vorrichtung zur polypeptidreinigung |
CN109324143B (zh) * | 2018-10-19 | 2021-10-29 | 张骐 | 利用二维液相分离制备抗体类产品相关杂质的方法 |
BR112021026256A2 (pt) | 2019-06-28 | 2022-03-03 | Vapotherm Inc | Cânula de geometria variável |
MX2022003715A (es) | 2019-09-26 | 2022-07-11 | Vapotherm Inc | Nebulizador montado en cánula interna. |
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CA1201063A (en) | 1982-07-20 | 1986-02-25 | Winnipeg Rh Institute Inc., (The) | Process for preparing purified immune globulin (igg) |
US5089605A (en) | 1987-03-13 | 1992-02-18 | Repligen Corporation | Immobilized immunoglobulin-binding proteins |
EP0289129A3 (de) | 1987-03-26 | 1990-10-10 | Repligen Corporation | Herstellung von hochgereinigtem Protein A |
US5084559A (en) * | 1987-03-27 | 1992-01-28 | Repligen Corporation | Protein a domain mutants |
US5118796A (en) | 1987-12-09 | 1992-06-02 | Centocor, Incorporated | Efficient large-scale purification of immunoglobulins and derivatives |
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WO1992018629A1 (en) | 1991-04-19 | 1992-10-29 | Genetics Institute, Inc. | Recombinant 3f8-type antibodies |
EP0752248B1 (de) * | 1992-11-13 | 2000-09-27 | Idec Pharmaceuticals Corporation | Therapeutische Verwendung von chimerischen und markierten Antikörpern, die gegen ein Differenzierung-Antigen gerichtet sind, dessen Expression auf menschliche B Lymphozyt beschränkt ist, für die Behandlung von B-Zell-Lymphoma |
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GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
EP1601697B1 (de) | 2003-02-28 | 2007-05-30 | Lonza Biologics plc | Reinigung von Antikörpern durch Protein A und Ionenaustauschromatographie |
JPWO2004087761A1 (ja) * | 2003-03-31 | 2006-07-27 | 麒麟麦酒株式会社 | ヒトモノクローナル抗体およびヒトポリクローナル抗体の精製 |
AU2004286938B2 (en) * | 2003-10-27 | 2011-06-30 | Wyeth | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
KR20070072510A (ko) | 2004-08-30 | 2007-07-04 | 론자 바이올로직스 피엘씨 | 항체 정제를 위한 친화도- 및 이온 교환- 크로마토그래피 |
-
2003
- 2003-02-28 GB GBGB0304576.2A patent/GB0304576D0/en not_active Ceased
-
2004
- 2004-03-01 DE DE602004006725T patent/DE602004006725T2/de not_active Expired - Lifetime
- 2004-03-01 ES ES04715906T patent/ES2288252T3/es not_active Expired - Lifetime
- 2004-03-01 AT AT04715906T patent/ATE363491T1/de active
- 2004-03-01 CN CNB2004800093477A patent/CN100384874C/zh not_active Expired - Lifetime
- 2004-03-01 JP JP2006501980A patent/JP2007525412A/ja not_active Withdrawn
- 2004-03-01 KR KR1020057015912A patent/KR101200732B1/ko not_active IP Right Cessation
- 2004-03-11 TW TW093106464A patent/TWI325428B/zh not_active IP Right Cessation
-
2005
- 2005-08-25 US US11/210,669 patent/US20060030696A1/en not_active Abandoned
-
2006
- 2006-05-19 US US11/419,306 patent/US7847071B2/en not_active Expired - Lifetime
-
2010
- 2010-10-22 US US12/910,301 patent/US20110040075A1/en not_active Abandoned
-
2011
- 2011-10-21 JP JP2011231797A patent/JP5752558B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US20060030696A1 (en) | 2006-02-09 |
CN100384874C (zh) | 2008-04-30 |
JP5752558B2 (ja) | 2015-07-22 |
US20060194953A1 (en) | 2006-08-31 |
US7847071B2 (en) | 2010-12-07 |
CN1771260A (zh) | 2006-05-10 |
ES2288252T3 (es) | 2008-01-01 |
KR101200732B1 (ko) | 2012-11-13 |
US20110040075A1 (en) | 2011-02-17 |
DE602004006725T2 (de) | 2008-02-07 |
JP2007525412A (ja) | 2007-09-06 |
ATE363491T1 (de) | 2007-06-15 |
GB0304576D0 (en) | 2003-04-02 |
JP2012067108A (ja) | 2012-04-05 |
KR20050113617A (ko) | 2005-12-02 |
TW200530265A (en) | 2005-09-16 |
TWI325428B (en) | 2010-06-01 |
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