DK1456228T3 - Fremgangsmåde til isolering og rensning af trypsin fra pronase samt dens anvendelse - Google Patents

Fremgangsmåde til isolering og rensning af trypsin fra pronase samt dens anvendelse

Info

Publication number
DK1456228T3
DK1456228T3 DK02798324.6T DK02798324T DK1456228T3 DK 1456228 T3 DK1456228 T3 DK 1456228T3 DK 02798324 T DK02798324 T DK 02798324T DK 1456228 T3 DK1456228 T3 DK 1456228T3
Authority
DK
Denmark
Prior art keywords
pronase
isolating
well
trypsin
purifying
Prior art date
Application number
DK02798324.6T
Other languages
English (en)
Inventor
Wolfgang Mundt
Artur Mitterer
Manfred Reiter
Christa Tauer
Original Assignee
Baxter Healthcare Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Healthcare Sa filed Critical Baxter Healthcare Sa
Application granted granted Critical
Publication of DK1456228T3 publication Critical patent/DK1456228T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32451—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK02798324.6T 2001-12-10 2002-12-10 Fremgangsmåde til isolering og rensning af trypsin fra pronase samt dens anvendelse DK1456228T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/006,223 US6830917B2 (en) 2001-12-10 2001-12-10 Method of isolation and purification of trypsin from pronase protease and use thereof
PCT/EP2002/014005 WO2003050275A2 (en) 2001-12-10 2002-12-10 Method of isolation and purification of trypsin from pronase and use thereof

Publications (1)

Publication Number Publication Date
DK1456228T3 true DK1456228T3 (da) 2011-08-15

Family

ID=21719866

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02798324.6T DK1456228T3 (da) 2001-12-10 2002-12-10 Fremgangsmåde til isolering og rensning af trypsin fra pronase samt dens anvendelse

Country Status (12)

Country Link
US (4) US6830917B2 (da)
EP (1) EP1456228B1 (da)
JP (2) JP4629336B2 (da)
CN (2) CN101348780B (da)
AT (1) ATE508137T1 (da)
AU (2) AU2002363865B2 (da)
CA (1) CA2469484C (da)
DE (1) DE60239957D1 (da)
DK (1) DK1456228T3 (da)
MX (1) MXPA04005639A (da)
NO (1) NO335427B1 (da)
WO (1) WO2003050275A2 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100687884B1 (ko) 2005-12-30 2007-02-27 한국과학기술원 스트렙토마이시스 그리세우스 유래의 조절유전자 ｓｇｔＲ1및 ｓｇｔＲ2를 이용한 재조합 트립신의 대량 생산방법
JP6021218B2 (ja) * 2012-06-25 2016-11-09 多木化学株式会社 コラーゲン誘導体の製造方法
AU2014346507B2 (en) 2013-11-08 2018-08-02 Precision Planting Llc Implement weight management systems, methods, and apparatus
CN108333358B (zh) * 2017-01-19 2020-05-01 深圳市新产业生物医学工程股份有限公司 一种免疫亲和纯化洗脱液的筛选方法
CN112143693A (zh) * 2019-06-28 2020-12-29 杭州康万达医药科技有限公司 一种生产病毒的方法及收获液组合物
CN110343689B (zh) * 2019-08-23 2021-11-05 四川大学 一种链霉菌胰蛋白酶gm2938及其在枯草芽孢杆菌中的异源表达

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE445466B (sv) * 1977-12-01 1986-06-23 Wellcome Found Sett att foroka rotavirus in vitro
CA1122527A (en) * 1979-05-15 1982-04-27 Karen K. Brown Influenza vaccine production in liquid cell culture
US4500513A (en) * 1979-05-15 1985-02-19 Miles Laboratories, Inc. Influenza vaccine production in liquid cell culture
US4973554A (en) * 1987-05-29 1990-11-27 National Research Council Of Canada Affinity process for trypsin purification and stabilization
JPH02268681A (ja) * 1989-04-07 1990-11-02 Green Cross Corp:The ウロキナーゼ前駆体の安定化方法及び乾燥製剤
RU2082431C1 (ru) * 1993-05-12 1997-06-27 Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им.Пастера Способ получения инактивированной гриппозной вакцины
US5824536A (en) * 1994-08-23 1998-10-20 St. Jude Children's Research Hospital Influenza virus replicated in mammalian cell culture and vaccine production
DK0791055T3 (da) * 1994-11-10 2012-04-16 Baxter Healthcare Sa Fremgangsmåde til fremstilling af biologiske produkter i proteinfri kultur
US5989805A (en) * 1995-10-27 1999-11-23 Board Of Trustees Operating Michigan State University Immortal avian cell line to grow avian and animal viruses to produce vaccines
AT404597B (de) 1995-11-24 1998-12-28 Immuno Ag Verfahren zur herstellung von proteinen
US5905042A (en) * 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US6168944B1 (en) * 1997-01-31 2001-01-02 Schering Corporation Methods for cultivating cells and propagating viruses
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
JP5149470B2 (ja) * 1999-02-22 2013-02-20 バクスター・インターナショナル・インコーポレイテッド 新規のアルブミンを含有していない第ｖｉｉｉ因子処方物
US20030032146A1 (en) * 2001-07-10 2003-02-13 Dwulet Francis Edward Enzyme/tag binding and detection system
US6825027B2 (en) * 2001-12-10 2004-11-30 Baxter Healthcare S.A. Method of production of purified hepatitis a virus particles and vaccine preparation

Also Published As

Publication number Publication date
EP1456228A2 (en) 2004-09-15
EP1456228B1 (en) 2011-05-04
JP4629336B2 (ja) 2011-02-09
ATE508137T1 (de) 2011-05-15
AU2002363865A1 (en) 2003-06-23
US20030124650A1 (en) 2003-07-03
US6830917B2 (en) 2004-12-14
US20070249015A1 (en) 2007-10-25
AU2008229752A1 (en) 2008-10-30
CN1617882B (zh) 2010-05-12
CN1617882A (zh) 2005-05-18
CN101348780B (zh) 2013-02-06
JP2005511087A (ja) 2005-04-28
CA2469484C (en) 2013-09-10
WO2003050275A8 (en) 2004-05-21
NO335427B1 (no) 2014-12-15
JP2010268799A (ja) 2010-12-02
CN101348780A (zh) 2009-01-21
AU2002363865B2 (en) 2008-07-03
US8389261B2 (en) 2013-03-05
NO20042908L (no) 2004-07-09
DE60239957D1 (de) 2011-06-16
US20120003691A1 (en) 2012-01-05
US7223595B2 (en) 2007-05-29
CA2469484A1 (en) 2003-06-19
WO2003050275A3 (en) 2004-02-19
US7659380B2 (en) 2010-02-09
AU2008229752B2 (en) 2011-02-17
MXPA04005639A (es) 2005-04-19
US20050069534A1 (en) 2005-03-31
WO2003050275A2 (en) 2003-06-19

Similar Documents

Publication Publication Date Title
GB0304576D0 (en) Protein a chromatography
DK1809663T3 (da) Fremgangsmåde til oprensning af FSH
IL185731A0 (en) Purification of montelukast
WO2004024866A3 (en) Protein purification
DK1702066T3 (da) Fremgangsmåde og anordning til oprensning af nukleinsyrer
MXPA06000407A (es) Isomero c cci-779.
AU2002359522A8 (en) Compositions and methods of selective nucleic acid isolation
DK1689777T3 (da) Fremgangsmåde til oprensning af il18-bindende protein
DK1084146T3 (da) Hybridprotein til inhibering af degranuleringen af mastocyter og anvendelse deraf
BRPI0312521B8 (pt) método para a separação de fibrinogênio de plasminogênio
SI1590061T1 (sl) Metoda za äśiĺ äśenje alfa-1-antitripsina
DK0975790T3 (da) Ny fremgangsmåde til isolering af polysaccharider
EA200601029A1 (ru) Способ очистки фсг
TW200420231A (en) Manufacture method of sugar chain asparagine derivatives
DK1456228T3 (da) Fremgangsmåde til isolering og rensning af trypsin fra pronase samt dens anvendelse
HK1047754A1 (en) Separation of fibrinogen from plasma proteases
DK1399551T3 (da) Fremgangsmåde til oprensning af bakterieceller og cellebestanddele
EP1450925A4 (en) PROCESS FOR SEPARATING COMPOUND (S) FROM MIX (S)
SE0303532D0 (sv) Purification of immunoglobulins
SE0401665D0 (sv) Purification of immunoglobulins
IS7288A (is) Aðferðir til að hreinsa levófloxasín
BRPI0416201A (pt) métodos para purificação de toxina coqueluche e peptìdios neles utilizáveis
MY137572A (en) Antibody purification by protein a and ion exchange chromatography
AU2003208649A1 (en) Method of extracting, isolating and purifying sesame protein
TW200722435A (en) Purification of proteins with cationic surfactant