DE60239957D1 - Verfahren zur isolierung und reinigung von trypsin aus pronase und ihre verwendung - Google Patents

Verfahren zur isolierung und reinigung von trypsin aus pronase und ihre verwendung

Info

Publication number
DE60239957D1
DE60239957D1 DE60239957T DE60239957T DE60239957D1 DE 60239957 D1 DE60239957 D1 DE 60239957D1 DE 60239957 T DE60239957 T DE 60239957T DE 60239957 T DE60239957 T DE 60239957T DE 60239957 D1 DE60239957 D1 DE 60239957D1
Authority
DE
Germany
Prior art keywords
pronase
trypsine
isolating
cleaning
sgt
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60239957T
Other languages
English (en)
Inventor
Artur Mitterer
Christa Tauer
Manfred Reiter
Wolfgang Mundt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter Healthcare SA
Original Assignee
Baxter Healthcare SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Healthcare SA filed Critical Baxter Healthcare SA
Application granted granted Critical
Publication of DE60239957D1 publication Critical patent/DE60239957D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32451—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE60239957T 2001-12-10 2002-12-10 Verfahren zur isolierung und reinigung von trypsin aus pronase und ihre verwendung Expired - Lifetime DE60239957D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/006,223 US6830917B2 (en) 2001-12-10 2001-12-10 Method of isolation and purification of trypsin from pronase protease and use thereof
PCT/EP2002/014005 WO2003050275A2 (en) 2001-12-10 2002-12-10 Method of isolation and purification of trypsin from pronase and use thereof

Publications (1)

Publication Number Publication Date
DE60239957D1 true DE60239957D1 (de) 2011-06-16

Family

ID=21719866

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60239957T Expired - Lifetime DE60239957D1 (de) 2001-12-10 2002-12-10 Verfahren zur isolierung und reinigung von trypsin aus pronase und ihre verwendung

Country Status (12)

Country Link
US (4) US6830917B2 (de)
EP (1) EP1456228B1 (de)
JP (2) JP4629336B2 (de)
CN (2) CN1617882B (de)
AT (1) ATE508137T1 (de)
AU (2) AU2002363865B2 (de)
CA (1) CA2469484C (de)
DE (1) DE60239957D1 (de)
DK (1) DK1456228T3 (de)
MX (1) MXPA04005639A (de)
NO (1) NO335427B1 (de)
WO (1) WO2003050275A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100687884B1 (ko) 2005-12-30 2007-02-27 한국과학기술원 스트렙토마이시스 그리세우스 유래의 조절유전자 ｓｇｔＲ1및 ｓｇｔＲ2를 이용한 재조합 트립신의 대량 생산방법
JP6021218B2 (ja) * 2012-06-25 2016-11-09 多木化学株式会社 コラーゲン誘導体の製造方法
BR112016010202B1 (pt) 2013-11-08 2021-01-26 Precision Planting Llc sistema de controle de transferência de peso e método para transferir um peso de implemento
CN108333358B (zh) * 2017-01-19 2020-05-01 深圳市新产业生物医学工程股份有限公司 一种免疫亲和纯化洗脱液的筛选方法
CN112143693A (zh) * 2019-06-28 2020-12-29 杭州康万达医药科技有限公司 一种生产病毒的方法及收获液组合物
CN110343689B (zh) * 2019-08-23 2021-11-05 四川大学 一种链霉菌胰蛋白酶gm2938及其在枯草芽孢杆菌中的异源表达

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE445466B (sv) * 1977-12-01 1986-06-23 Wellcome Found Sett att foroka rotavirus in vitro
US4500513A (en) * 1979-05-15 1985-02-19 Miles Laboratories, Inc. Influenza vaccine production in liquid cell culture
CA1122527A (en) * 1979-05-15 1982-04-27 Karen K. Brown Influenza vaccine production in liquid cell culture
US4973554A (en) * 1987-05-29 1990-11-27 National Research Council Of Canada Affinity process for trypsin purification and stabilization
JPH02268681A (ja) * 1989-04-07 1990-11-02 Green Cross Corp:The ウロキナーゼ前駆体の安定化方法及び乾燥製剤
RU2082431C1 (ru) * 1993-05-12 1997-06-27 Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им.Пастера Способ получения инактивированной гриппозной вакцины
US5824536A (en) * 1994-08-23 1998-10-20 St. Jude Children's Research Hospital Influenza virus replicated in mammalian cell culture and vaccine production
WO1996015231A2 (en) 1994-11-10 1996-05-23 Immuno Aktiengesellschaft Method for producing biologicals in protein-free culture
US5989805A (en) * 1995-10-27 1999-11-23 Board Of Trustees Operating Michigan State University Immortal avian cell line to grow avian and animal viruses to produce vaccines
AT404597B (de) 1995-11-24 1998-12-28 Immuno Ag Verfahren zur herstellung von proteinen
US5905042A (en) * 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US6168944B1 (en) * 1997-01-31 2001-01-02 Schering Corporation Methods for cultivating cells and propagating viruses
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
DK1820516T3 (da) * 1999-02-22 2013-10-28 Univ Connecticut Nye albuminfrie faktor VIII-præparater
US20030032146A1 (en) * 2001-07-10 2003-02-13 Dwulet Francis Edward Enzyme/tag binding and detection system
US6825027B2 (en) * 2001-12-10 2004-11-30 Baxter Healthcare S.A. Method of production of purified hepatitis a virus particles and vaccine preparation

Also Published As

Publication number Publication date
WO2003050275A2 (en) 2003-06-19
CN1617882B (zh) 2010-05-12
NO335427B1 (no) 2014-12-15
JP4629336B2 (ja) 2011-02-09
DK1456228T3 (da) 2011-08-15
US20120003691A1 (en) 2012-01-05
CN101348780B (zh) 2013-02-06
AU2008229752A1 (en) 2008-10-30
JP2010268799A (ja) 2010-12-02
CA2469484A1 (en) 2003-06-19
WO2003050275A8 (en) 2004-05-21
AU2002363865A1 (en) 2003-06-23
EP1456228B1 (de) 2011-05-04
US20030124650A1 (en) 2003-07-03
AU2008229752B2 (en) 2011-02-17
US20070249015A1 (en) 2007-10-25
US8389261B2 (en) 2013-03-05
CN101348780A (zh) 2009-01-21
US7223595B2 (en) 2007-05-29
CA2469484C (en) 2013-09-10
WO2003050275A3 (en) 2004-02-19
US20050069534A1 (en) 2005-03-31
EP1456228A2 (de) 2004-09-15
ATE508137T1 (de) 2011-05-15
US6830917B2 (en) 2004-12-14
CN1617882A (zh) 2005-05-18
AU2002363865B2 (en) 2008-07-03
NO20042908L (no) 2004-07-09
MXPA04005639A (es) 2005-04-19
US7659380B2 (en) 2010-02-09
JP2005511087A (ja) 2005-04-28

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