MX357821B - Preparación de anticuerpo enriquecida con isoforma y método para obtenerla. - Google Patents

Preparación de anticuerpo enriquecida con isoforma y método para obtenerla.

Info

Publication number
MX357821B
MX357821B MX2013007095A MX2013007095A MX357821B MX 357821 B MX357821 B MX 357821B MX 2013007095 A MX2013007095 A MX 2013007095A MX 2013007095 A MX2013007095 A MX 2013007095A MX 357821 B MX357821 B MX 357821B
Authority
MX
Mexico
Prior art keywords
obtaining
antibody preparation
solution
conductivity
cation exchange
Prior art date
Application number
MX2013007095A
Other languages
English (en)
Other versions
MX2013007095A (es
Inventor
Roberto Falkenstein
Klaus Schwendner
Bernhard Spensberger
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43799680&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX357821(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2013007095A publication Critical patent/MX2013007095A/es
Publication of MX357821B publication Critical patent/MX357821B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3015—Breast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La presente invención se refiere a un método para producir una preparación de anticuerpo que comprende las etapas de a) aplicar una solución amortiguada que comprende diferentes isoformas de un anticuerpo a un material de cromatografía de intercambio de cationes, b) aplicar una primera solución con una primera conductividad al material de cromatografía de intercambio de cationes, con lo que las isoformas de anticuerpos permanecen ligadas al material de cromatografía de intercambio de cationes, y c) aplicar una segunda solución con una segunda conductividad al material de cromatografía de intercambio de cationes y de esta manera obtener la preparación de anticuerpos, con lo que la conductividad de la segunda solución excede la conductividad de la primera solución en no más de 10%.
MX2013007095A 2010-12-21 2011-12-19 Preparación de anticuerpo enriquecida con isoforma y método para obtenerla. MX357821B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10196287 2010-12-21
PCT/EP2011/073245 WO2012084829A1 (en) 2010-12-21 2011-12-19 Isoform enriched antibody preparation and method for obtaining it

Publications (2)

Publication Number Publication Date
MX2013007095A MX2013007095A (es) 2013-07-29
MX357821B true MX357821B (es) 2018-07-25

Family

ID=43799680

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013007095A MX357821B (es) 2010-12-21 2011-12-19 Preparación de anticuerpo enriquecida con isoforma y método para obtenerla.

Country Status (10)

Country Link
US (2) US20140162317A1 (es)
EP (1) EP2655412B1 (es)
JP (1) JP5838221B2 (es)
KR (1) KR101574864B1 (es)
CN (1) CN103380144B (es)
BR (1) BR112013012422A2 (es)
CA (1) CA2820095C (es)
MX (1) MX357821B (es)
RU (1) RU2586515C2 (es)
WO (1) WO2012084829A1 (es)

Families Citing this family (12)

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MX346523B (es) 2006-09-13 2017-03-23 Abbvie Inc Mejoras de cultivos celulares.
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
NZ592097A (en) 2008-10-20 2013-01-25 Abbott Lab Viral inactivation during purification of il-12 and il-18 antibodies
WO2010141039A1 (en) 2008-10-20 2010-12-09 Abbott Laboratories Isolation and purification of antibodies using protein a affinity chromatography
CA2890979A1 (en) 2012-11-15 2014-05-22 Genentech, Inc. Ionic strength-mediated ph gradient ion exchange chromatography
WO2015064971A1 (ko) * 2013-10-30 2015-05-07 (주)셀트리온 양이온 교환 크로마토그래피를 이용한 항체의 아형 분리 방법
WO2015083853A1 (ko) * 2013-12-05 2015-06-11 한화케미칼 주식회사 이성질 항체의 함량 조절을 통한 항체 제조 방법
JP2021006540A (ja) * 2014-09-10 2021-01-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ガラクトース操作型免疫グロブリン１抗体
JP2019504060A (ja) * 2016-01-08 2019-02-14 オンコバイオロジクス，インコーポレイティド モノクローナル抗体のアイソフォームを分離するための方法
AR111465A1 (es) * 2017-04-14 2019-07-17 Cj Healthcare Corp Método para la purificación de anticuerpos análogos por el uso de cromatografía de intercambio de cationes
MA50534A (fr) 2017-11-01 2020-09-09 Chugai Pharmaceutical Co Ltd Variant d'anticorps et isoforme à activité biologique réduite
CN115728433B (zh) * 2021-08-27 2024-11-15 联邦生物科技(珠海横琴)有限公司 IgG4型单克隆抗体电荷异质性的离子交换色谱检测方法

Family Cites Families (24)

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US5202238A (en) 1987-10-27 1993-04-13 Oncogen Production of chimeric antibodies by homologous recombination
US5204244A (en) 1987-10-27 1993-04-20 Oncogen Production of chimeric antibodies by homologous recombination
WO1989006692A1 (en) 1988-01-12 1989-07-27 Genentech, Inc. Method of treating tumor cells by inhibiting growth factor receptor function
US6407213B1 (en) 1991-06-14 2002-06-18 Genentech, Inc. Method for making humanized antibodies
AU4025193A (en) 1992-04-08 1993-11-18 Cetus Oncology Corporation Humanized C-erbB-2 specific antibodies
CA2120745A1 (en) 1992-06-30 1994-01-06 Philip G. Kasprzyk A combination of anti-erbb-2 monoclonal antibodies and method of using
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
DK2275119T3 (da) 1995-07-27 2013-11-11 Genentech Inc Stabil, isotonisk lyofiliseret proteinformulering
US5783186A (en) 1995-12-05 1998-07-21 Amgen Inc. Antibody-induced apoptosis
CN1305896C (zh) 1998-05-06 2007-03-21 基因技术股份有限公司 用离子交换层析纯化蛋白质
BRPI0012198B8 (pt) 1999-06-25 2021-05-25 Genentech Inc anticorpos humanizados, composição e imunoconjugado
CA2473144C (en) 2002-02-05 2013-05-28 Genentech, Inc. Protein purification
EP2332996B1 (en) * 2002-09-11 2014-10-15 Genentech, Inc. Purification of anti-Her2 antibodies
AU2004215653B2 (en) 2003-02-28 2011-03-17 Lonza Biologics Plc. Antibody purification by protein A and ion exchange chromatography
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
CA2582113C (en) * 2004-10-21 2013-07-30 Ge Healthcare Bio-Sciences Ab A method of antibody purification
US8129508B2 (en) * 2005-04-11 2012-03-06 Medarex, Inc. Protein purification using HCIC and ion exchange chromatography
TWI320788B (en) * 2005-05-25 2010-02-21 Hoffmann La Roche Method for the purification of antibodies
KR20150064254A (ko) 2006-04-05 2015-06-10 애브비 바이오테크놀로지 리미티드 항체 정제
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CA2768325C (en) * 2009-07-24 2019-10-29 F. Hoffmann-La Roche Ag Optimizing the production of antibodies

Also Published As

Publication number Publication date
CN103380144A (zh) 2013-10-30
CA2820095A1 (en) 2012-06-28
KR20130114209A (ko) 2013-10-16
BR112013012422A2 (pt) 2016-08-30
RU2586515C2 (ru) 2016-06-10
EP2655412A1 (en) 2013-10-30
JP5838221B2 (ja) 2016-01-06
US10087237B2 (en) 2018-10-02
US20170066814A1 (en) 2017-03-09
EP2655412B1 (en) 2018-01-17
CN103380144B (zh) 2016-03-02
KR101574864B1 (ko) 2015-12-11
WO2012084829A1 (en) 2012-06-28
JP2014500289A (ja) 2014-01-09
CA2820095C (en) 2019-02-26
HK1186484A1 (zh) 2014-03-14
MX2013007095A (es) 2013-07-29
RU2013132405A (ru) 2015-01-27
US20140162317A1 (en) 2014-06-12

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