DE60121342T2 - Einfach markierte oligonukleotidsonden - Google Patents

Einfach markierte oligonukleotidsonden Download PDF

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Publication number
DE60121342T2
DE60121342T2 DE60121342T DE60121342T DE60121342T2 DE 60121342 T2 DE60121342 T2 DE 60121342T2 DE 60121342 T DE60121342 T DE 60121342T DE 60121342 T DE60121342 T DE 60121342T DE 60121342 T2 DE60121342 T2 DE 60121342T2
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DE
Germany
Prior art keywords
probe
nucleic acid
fluorescence
hybridization
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60121342T
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German (de)
English (en)
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DE60121342D1 (de
Inventor
T. Carl Salt Lake City WITTWER
O. Andrew West Valley CROCKETT
E. Brian Salt Lake City CAPLIN
Wade West Jordan STEVENSON
A. Lori Salt Lake City WAGNER
Jian Sandy CHEN
Noriko Salt Lake City KUSUKAWA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biofire Defense LLC
University of Utah Research Foundation Inc
Original Assignee
University of Utah Research Foundation Inc
Idaho Technology Inc
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Publication of DE60121342D1 publication Critical patent/DE60121342D1/de
Application granted granted Critical
Publication of DE60121342T2 publication Critical patent/DE60121342T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
DE60121342T 2000-08-11 2001-08-10 Einfach markierte oligonukleotidsonden Expired - Lifetime DE60121342T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22472600P 2000-08-11 2000-08-11
US224726P 2000-08-11
US24061000P 2000-10-16 2000-10-16
US240610P 2000-10-16
PCT/US2001/025231 WO2002014555A2 (en) 2000-08-11 2001-08-10 Single-labeled oligonucleotide probes

Publications (2)

Publication Number Publication Date
DE60121342D1 DE60121342D1 (de) 2006-08-17
DE60121342T2 true DE60121342T2 (de) 2007-06-28

Family

ID=26918967

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60121342T Expired - Lifetime DE60121342T2 (de) 2000-08-11 2001-08-10 Einfach markierte oligonukleotidsonden

Country Status (8)

Country Link
US (1) US6635427B2 (https=)
EP (1) EP1307592B1 (https=)
JP (1) JP5213297B2 (https=)
AT (1) ATE332398T1 (https=)
CA (1) CA2417986C (https=)
DE (1) DE60121342T2 (https=)
ES (1) ES2267803T3 (https=)
WO (1) WO2002014555A2 (https=)

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Also Published As

Publication number Publication date
DE60121342D1 (de) 2006-08-17
ES2267803T3 (es) 2007-03-16
CA2417986C (en) 2013-11-26
US6635427B2 (en) 2003-10-21
WO2002014555A2 (en) 2002-02-21
ATE332398T1 (de) 2006-07-15
EP1307592B1 (en) 2006-07-05
EP1307592A2 (en) 2003-05-07
JP5213297B2 (ja) 2013-06-19
WO2002014555A3 (en) 2003-02-27
JP2004506431A (ja) 2004-03-04
CA2417986A1 (en) 2002-02-21
US20030022177A1 (en) 2003-01-30

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