ATE332398T1 - Einfach markierte oligonukleotidsonden - Google Patents

Einfach markierte oligonukleotidsonden

Info

Publication number
ATE332398T1
ATE332398T1 AT01959722T AT01959722T ATE332398T1 AT E332398 T1 ATE332398 T1 AT E332398T1 AT 01959722 T AT01959722 T AT 01959722T AT 01959722 T AT01959722 T AT 01959722T AT E332398 T1 ATE332398 T1 AT E332398T1
Authority
AT
Austria
Prior art keywords
probes
nucleic acid
methods
labeled oligonucleotide
oligonucleotide probe
Prior art date
Application number
AT01959722T
Other languages
German (de)
English (en)
Inventor
Carl T Wittwer
Andrew O Crockett
Brian E Caplin
Wade Stevenson
Lori A Wagner
Jian Chen
Noriko Kusukawa
Original Assignee
Univ Utah Res Found
Idaho Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah Res Found, Idaho Technology Inc filed Critical Univ Utah Res Found
Application granted granted Critical
Publication of ATE332398T1 publication Critical patent/ATE332398T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
AT01959722T 2000-08-11 2001-08-10 Einfach markierte oligonukleotidsonden ATE332398T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22472600P 2000-08-11 2000-08-11
US24061000P 2000-10-16 2000-10-16

Publications (1)

Publication Number Publication Date
ATE332398T1 true ATE332398T1 (de) 2006-07-15

Family

ID=26918967

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01959722T ATE332398T1 (de) 2000-08-11 2001-08-10 Einfach markierte oligonukleotidsonden

Country Status (8)

Country Link
US (1) US6635427B2 (https=)
EP (1) EP1307592B1 (https=)
JP (1) JP5213297B2 (https=)
AT (1) ATE332398T1 (https=)
CA (1) CA2417986C (https=)
DE (1) DE60121342T2 (https=)
ES (1) ES2267803T3 (https=)
WO (1) WO2002014555A2 (https=)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
EP1715063B1 (en) 2000-03-29 2011-01-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US7998673B2 (en) 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US8409508B2 (en) 2002-04-23 2013-04-02 Biofire Diagnostics, Inc. Sample withdrawal and dispensing device
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7122384B2 (en) * 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
DE60333480D1 (de) 2002-12-06 2010-09-02 Roche Diagnostics Gmbh Verfahren zum nachweis von pathogenen organismen
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
ATE550099T1 (de) 2003-04-04 2012-04-15 Hoffmann La Roche Verbessertes system für mehrfarbige echtzeit-pcr
JP4454249B2 (ja) 2003-04-18 2010-04-21 アークレイ株式会社 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP1689764B1 (en) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Oligonucleotides labeled with a plurality of fluorophores
FR2866349B1 (fr) * 2004-02-12 2008-05-16 Biomerieux Sa Procede de diagnostic/pronostic d'une thrombose
US20080032413A1 (en) * 2004-04-12 2008-02-07 Byeang-Hyean Kim Oligonucleotide For Detecting Target Dna Or Rna
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
AU2006204087B2 (en) * 2005-01-03 2010-09-16 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
DE602005000877T2 (de) 2005-01-18 2007-12-20 Roche Diagnostics Gmbh Fluoreszenzabbildung mittels Telezentrizität
ATE357653T1 (de) 2005-01-18 2007-04-15 Hoffmann La Roche Fluoreszenzabbildung mittels telezentrischer anregungs- und abbildungsoptiken
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
EP1801114B8 (de) 2005-11-23 2009-12-02 Roche Diagnostics GmbH Polynukleotid mit Phosphatmimetikum
JP4713514B2 (ja) * 2006-02-20 2011-06-29 エフ．ホフマン−ラ ロシュ アーゲー 改善された標識試薬
ATE510027T1 (de) 2006-02-27 2011-06-15 Hoffmann La Roche Pcr-heissstart durch magnesium-sequestrierung
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
WO2008020090A1 (es) * 2006-08-18 2008-02-21 Laboratorios Indas, S.A. Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa
EP2857526B1 (en) 2006-12-13 2016-08-17 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
US8247171B2 (en) * 2007-02-01 2012-08-21 Abacus Diagnostica Oy Method for detection of presence of target polynucleotide in samples
JPWO2009011297A1 (ja) * 2007-07-13 2010-09-24 アークレイ株式会社 Ｊａｋ２遺伝子の変異検出用プローブおよびその用途
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
AU2009262870B2 (en) 2008-05-30 2014-03-20 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
WO2010018245A1 (es) * 2008-08-12 2010-02-18 Biotools Biotechnological & Medical Laboratories, S.A. Método para la detección y/o cuantificación de un ácido nucleico substrato
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
EP2436777B1 (en) 2009-05-26 2015-08-19 Xiamen University Method for detecting variations in nucleic acid sequences
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
WO2011052754A1 (ja) * 2009-10-30 2011-05-05 アークレイ株式会社 Ｅｇｆｒ遺伝子多型検出用プローブおよびその用途
WO2011062258A1 (ja) * 2009-11-19 2011-05-26 アークレイ株式会社 Ｍｔｈｆｒ遺伝子増幅用プライマーセット、それを含むｍｔｈｆｒ遺伝子増幅用試薬およびその用途
CN102317454A (zh) * 2009-12-07 2012-01-11 爱科来株式会社 疾病相关基因的多态性检测用探针及其用途
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
US9285319B2 (en) 2010-04-21 2016-03-15 Riken Nucleic acid base analogs with quenching and fluorescent activities and applications thereof
JP5940771B2 (ja) * 2010-07-12 2016-06-29 アークレイ株式会社 ａｂｌ遺伝子多型の検出用プローブおよびその用途
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
US20120276533A1 (en) * 2011-04-28 2012-11-01 Arkray, Inc. Method for Simultaneously Detecting Polymorphisms of Acetaldehyde Dehydrogenase 2 and Alcohol Dehydrogenase 2
ES2547075T3 (es) 2011-06-16 2015-10-01 Gendiag.Exe, S.L. SNP asociados a enfermedad tromboembólica
US10557170B2 (en) 2011-06-16 2020-02-11 Gendiag.Exe, S.L. Thromboembolic disease markers
US20140248611A1 (en) * 2011-06-16 2014-09-04 Nippon Steel & Sumikin Eco-Tech Corporation Nucleic acid probe for assaying nucleic acids
US9366677B2 (en) * 2011-09-06 2016-06-14 Max-Planck-Gesellshaft Zur Foerderung Der Wissenschaften E.V. Methods for analyzing biological macromolecular complexes and use thereof
JP2013074888A (ja) 2011-09-15 2013-04-25 Arkray Inc ＩＬ２８Ｂ（ｒｓ８０９９９１７）とＩＴＰＡ（ｒｓ１１２７３５４）の変異を検出する方法
US20150126438A1 (en) 2012-01-24 2015-05-07 Beth Israel Deaconess Medical Center, Inc. Novel ChREBP Isoforms and Methods Using the Same
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP2948566B1 (en) 2013-01-24 2025-03-26 California Institute of Technology Chromophore-based characterization and detection methods
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
WO2014189843A1 (en) 2013-05-20 2014-11-27 Board Of Trustees Of The University Of Arkansas Gep5 model for multiple myeloma
GB201319180D0 (en) * 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
WO2015200855A1 (en) 2014-06-26 2015-12-30 University Of Florida Research Foundation, Inc. Rapid detection of infectious agents
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
AU2016252998B2 (en) 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
CA3012815C (en) * 2016-02-09 2021-09-28 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid and nucleic acid probe used therein
JP7483373B2 (ja) 2016-07-29 2024-05-15 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法
EP3644721A1 (en) 2017-06-29 2020-05-06 Juno Therapeutics, Inc. Mouse model for assessing toxicities associated with immunotherapies
BR112020001719A2 (pt) 2017-07-29 2020-07-21 Juno Therapeutics Inc reagentes para expansão de células que expressam receptores recombinantes
WO2019046832A1 (en) 2017-09-01 2019-03-07 Juno Therapeutics, Inc. GENE EXPRESSION AND EVALUATION OF RISK OF DEVELOPMENT OF TOXICITY FOLLOWING CELL THERAPY
CN107727618B (zh) * 2017-09-04 2019-12-31 江苏大学 生物芯片和特异性切割位点dna序列检测汞离子的方法
SG11202003657VA (en) 2017-11-01 2020-05-28 Juno Therapeutics Inc Process for producing a t cell composition
US11535903B2 (en) 2018-01-31 2022-12-27 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
EP3877054B1 (en) 2018-11-06 2023-11-01 Juno Therapeutics, Inc. Process for producing genetically engineered t cells
WO2020113141A2 (en) 2018-11-30 2020-06-04 Alpine Immune Sciences, Inc. Cd86 variant immunomodulatory proteins and uses thereof
EP4438054A3 (en) 2019-04-17 2025-01-08 Alpine Immune Sciences, Inc. Methods and uses of variant icos ligand (icosl) fusion proteins
EP3983006A1 (en) 2019-06-12 2022-04-20 Juno Therapeutics, Inc. Combination therapy of a cell-mediated cytotoxic therapy and an inhibitor of a prosurvival bcl2 family protein
JP2022545467A (ja) 2019-08-22 2022-10-27 ジュノー セラピューティクス インコーポレイテッド Ｔ細胞療法とｚｅｓｔｅホモログ２エンハンサー（ＥＺＨ２）阻害剤との併用療法および関連方法
US20220340981A1 (en) * 2019-09-23 2022-10-27 Universiteit Gent Probe and method for str-genotyping
KR102107589B1 (ko) * 2019-11-20 2020-05-07 주식회사 유진셀 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
MX2022008625A (es) 2020-01-13 2022-09-09 Aspen Neuroscience Inc Metodo de diferenciacion de celulas neuronales y composiciones relacionadas y metodos de uso.
WO2021231661A2 (en) 2020-05-13 2021-11-18 Juno Therapeutics, Inc. Process for producing donor-batched cells expressing a recombinant receptor
EP4171616A1 (en) 2020-06-26 2023-05-03 Juno Therapeutics GmbH Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
US20240329032A1 (en) 2021-07-21 2024-10-03 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
CA3241326A1 (en) 2021-12-03 2023-06-08 Medicinal Genomics Corporation Psilocybe assay
EP4508645A1 (en) 2022-04-15 2025-02-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
EP4532695A1 (en) 2022-05-25 2025-04-09 Celgene Corporation Methods of manufacturing t cell therapies
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
EP0826066B1 (en) 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
GB9602025D0 (en) * 1996-02-01 1996-04-03 Amersham Int Plc Nucleoside analogues
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
CA2253969A1 (en) 1996-05-15 1997-11-20 Rajkumar Siva Iyer Non-nucleotide linking reagents
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
US6451530B1 (en) * 1996-12-13 2002-09-17 The United States Of America As Represented By The Department Of Health And Human Services Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
SE522077C2 (sv) * 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
WO1999011813A2 (en) * 1997-09-04 1999-03-11 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
DE19858588B4 (de) 1998-08-22 2016-04-07 Qiagen Gmbh Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls
CA2304260C (en) 1999-04-20 2009-03-24 Japan Bioindustry Association Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
SE9902565D0 (sv) * 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
EP1715063B1 (en) 2000-03-29 2011-01-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination

Also Published As

Publication number Publication date
DE60121342D1 (de) 2006-08-17
DE60121342T2 (de) 2007-06-28
ES2267803T3 (es) 2007-03-16
CA2417986C (en) 2013-11-26
US6635427B2 (en) 2003-10-21
WO2002014555A2 (en) 2002-02-21
EP1307592B1 (en) 2006-07-05
EP1307592A2 (en) 2003-05-07
JP5213297B2 (ja) 2013-06-19
WO2002014555A3 (en) 2003-02-27
JP2004506431A (ja) 2004-03-04
CA2417986A1 (en) 2002-02-21
US20030022177A1 (en) 2003-01-30

Similar Documents

Publication Publication Date Title
ATE332398T1 (de) Einfach markierte oligonukleotidsonden
Knemeyer et al. Probes for detection of specific DNA sequences at the single-molecule level
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
ATE443777T1 (de) Methoden und zusammensetzungen zur analyse von ribonukleinsäuren
WO2003008622A3 (en) Exponential nucleic acid amplification using nicking endonucleases
DE60035459D1 (de) Automatisierte Echtzeit-Analyse von Nukleinsäureamplifikation
JP2004506431A5 (https=)
DK1278889T3 (da) Hydridiseringsbeacon og fremgangsmåde til hurtig sekvensdetektion og distinktion
WO2003087297A3 (en) Infectious disease microarray
DE60136567D1 (de) Polynukleotid-proben zum nachweis und zur quantifizierung von candida spezies
ATE368125T1 (de) Verfahren zur erkennung von einzel-nukleotid- polymorphismen
PL1633888T3 (pl) Środki i sposoby detekcji docelowych sekwencji nukleotydowych z użyciem oznaczeń ligacji i par ulepszonych sond oligonukleotydowych
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
WO2005047468A3 (en) Improved methods for detecting and measuring specific nucleic acid sequences
ATE366325T1 (de) Verfahren zum nachweisen von mikroorganismen in einer probe
HUP0204549A2 (hu) Eljárás adenovírus kimutatására és mennyiségi meghatározására
DE60327345D1 (de) Nachweissystem
ATE197071T1 (de) Nachweis von nukleinsäure-amplifikation mittels fluoreszenz-polarisation
DE60330160D1 (de) Oligonukleotide zur Detektion von Hepatitis B Viren
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
DE60119902D1 (de) Verfahren für die gleichzeitige und mehrfache detektion und quantifizierung der hybridisierung von molekülverbindungen wie nukleinsäuren, dns, rns, pns und proteinen
DE60324819D1 (de) Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus
ATE383579T1 (de) Verkleinerung der fluoreszenzlöschung während einer bestimmung
GB2362212A (en) Method for the identification and speciation of bacteria of the burkholderia cepacia complex
CY1105861T1 (el) Μεθοδος για την ανιχνευση δευτepευοντων γονιδιωματων σε τυπους ιου χρησιμοποιωντας μικροτσιπ dna

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties