ATE474933T1 - Verfahren zum nachweis von pathogenen organismen - Google Patents

Verfahren zum nachweis von pathogenen organismen

Info

Publication number
ATE474933T1
ATE474933T1 AT03782268T AT03782268T ATE474933T1 AT E474933 T1 ATE474933 T1 AT E474933T1 AT 03782268 T AT03782268 T AT 03782268T AT 03782268 T AT03782268 T AT 03782268T AT E474933 T1 ATE474933 T1 AT E474933T1
Authority
AT
Austria
Prior art keywords
hybridization
pathogens
amplification
indicative
nucleic acid
Prior art date
Application number
AT03782268T
Other languages
English (en)
Inventor
Gerd Haberhausen
Thomas Emrich
Gregor Sagner
Martin Moczko
Gudrun Schmitz-Agheguian
Original Assignee
Hoffmann La Roche
Innogenetics Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP02027272A external-priority patent/EP1426447A1/de
Application filed by Hoffmann La Roche, Innogenetics Nv filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE474933T1 publication Critical patent/ATE474933T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Electrotherapy Devices (AREA)
  • Geophysics And Detection Of Objects (AREA)
AT03782268T 2002-12-06 2003-12-02 Verfahren zum nachweis von pathogenen organismen ATE474933T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02027272A EP1426447A1 (de) 2002-12-06 2002-12-06 Verfahren zur Detection des pathogenen gram positiven Bacterien, Bvw. Staphylococcus, Enterococcus and Streptococcus
EP03007458 2003-04-04
PCT/EP2003/013530 WO2004053155A1 (en) 2002-12-06 2003-12-02 Multiplex assay detection of pathogenic organisms

Publications (1)

Publication Number Publication Date
ATE474933T1 true ATE474933T1 (de) 2010-08-15

Family

ID=32510124

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03782268T ATE474933T1 (de) 2002-12-06 2003-12-02 Verfahren zum nachweis von pathogenen organismen

Country Status (9)

Country Link
US (1) US20060099596A1 (de)
EP (2) EP1570079B1 (de)
JP (2) JP4377375B2 (de)
CN (1) CN105349657A (de)
AT (1) ATE474933T1 (de)
AU (2) AU2003289927A1 (de)
CA (2) CA2507240C (de)
DE (1) DE60333480D1 (de)
WO (2) WO2004053155A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0316232A (pt) 2002-11-12 2005-10-04 Becton Dickinson Co Métodos para determinar o estado de sepsia, para prognosticar o começo de sepsia e para diagnosticar a sìndrome de resposta inflamatória sistêmica em um indivìduo
EP1575978A4 (de) 2002-11-12 2006-04-19 Becton Dickinson Co Sepsis- oder sirs-diagnose mittels biomarkerprofilen
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
EP1613771B1 (de) * 2003-04-04 2012-03-21 Roche Diagnostics GmbH Verbessertes system für mehrfarbige echtzeit-pcr
EP1869463A4 (de) 2005-04-15 2010-05-05 Becton Dickinson Co Sepsisdiagnose
US20090286691A1 (en) * 2006-01-20 2009-11-19 Genein Co., Ltd. Oligonucleotide for Detection of Bacteria Associated with Sepsis and Microarrays and Method for Detection of the Bacteria Using the Oligonucleotide
ES2426038T3 (es) 2006-02-21 2013-10-18 National University Corporation University Of Toyama Procedimiento rápido para identificar el microorganismo causante de una enfermedad infecciosa
US8535888B2 (en) * 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
DE102007041864B4 (de) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
WO2009123737A2 (en) 2008-04-03 2009-10-08 Becton, Dickinson And Company Advanced detection of sepsis
JP5709897B2 (ja) * 2009-12-24 2015-04-30 シージーン アイエヌシー 偽シグナルが排除されるターゲット核酸配列のリアルタイムマルチプレッキシング検出
EP2646569A1 (de) * 2010-11-30 2013-10-09 Diagon Kft. Verfahren für nukleinsäurebasierte molekulardiagnostische bestimmung von keimzählungen und kit hierfür
CN105121656A (zh) * 2012-11-15 2015-12-02 以色列分子检测有限公司 Pcr反应混合物及使用其的方法
KR101586678B1 (ko) * 2013-11-19 2016-01-21 한국표준과학연구원 단일 염기 다형성을 갖는 미생물 16S rDNA 유전자를 이용한 정량 분석 방법
CN107513494A (zh) * 2017-08-17 2017-12-26 湖北伽诺美生物科技有限公司 一种核酸检测卡及其使用方法
WO2019044126A1 (ja) * 2017-08-30 2019-03-07 東洋製罐グループホールディングス株式会社 微生物密度の推定方法、及び微生物密度推定用マイクロアレイ
CN113528684A (zh) * 2021-08-04 2021-10-22 领航基因科技(杭州)有限公司 用于鉴别革兰氏阴性菌和革兰氏阳性菌的数字pcr检测试剂盒和应用

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1180647A (en) 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
DE3486467T3 (de) 1983-01-10 2004-10-14 Gen-Probe Inc., San Diego Verfahren zum Nachweis, Identifizieren und Quantifizieren von Organismen und Viren
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
EP0452596A1 (de) * 1990-04-18 1991-10-23 N.V. Innogenetics S.A. Hibridizierungssonden aus dem Abstandsbereich zwischen den 16S- und 23S-rRNA-Genen und Nachweis von nichtviralen Mikroorganismen
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5750409A (en) 1991-11-18 1998-05-12 Boehringer Mannheim Gmbh Pentacyclic compounds and their use as absorption or fluorescent dyes
EP0769068B1 (de) * 1994-06-24 2005-10-12 Innogenetics N.V. Gleichzeitiger nachweis, identifizierung und differenzierung von eubakterien unter verwendung eines hybridisierungs-assays
US6001564A (en) * 1994-09-12 1999-12-14 Infectio Diagnostic, Inc. Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
WO1997007238A2 (de) 1995-08-17 1997-02-27 Eberhard-Karls-Universität Tübingen Extraktion, amplifikation und sequentielle hybridisierung von pilzzellen-dna sowie verfahren zum nachweisen von pilzzellen in klinischem material
US5849488A (en) * 1996-02-27 1998-12-15 Oulutech Ltd. DNA-sequence-based diagnosis of mastitis from a milk sample
CA2257109C (en) 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
JP4281877B2 (ja) 1996-06-04 2009-06-17 ユニバーシティ オブ ユタ リサーチ ファウンデーション 生物学的プロセスを実行し且つモニタリングするためのシステムと方法
DE10027113A1 (de) 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
ATE332398T1 (de) 2000-08-11 2006-07-15 Univ Utah Res Found Einfach markierte oligonukleotidsonden
DE10100493A1 (de) * 2001-01-08 2002-08-01 Biotecon Diagnostics Gmbh Nachweis von pathogenen Bakterien
AU2002305941A1 (en) * 2001-03-01 2002-09-19 The Johns Hopkins University Quantitative assay for the simultaneous detection and speciation of bacterial infections
GB2377017A (en) * 2001-06-28 2002-12-31 Animal Health Inst Detection of foot and mouth disease virus

Also Published As

Publication number Publication date
JP2006508694A (ja) 2006-03-16
CA2507240A1 (en) 2004-06-24
CA2507933A1 (en) 2004-06-24
CA2507933C (en) 2009-11-10
EP1570079A1 (de) 2005-09-07
EP1570086A4 (de) 2006-01-11
WO2004053457A3 (en) 2004-09-23
DE60333480D1 (de) 2010-09-02
EP1570086A2 (de) 2005-09-07
AU2003289927A1 (en) 2004-06-30
US20060099596A1 (en) 2006-05-11
JP2006518189A (ja) 2006-08-10
AU2003300825A1 (en) 2004-06-30
WO2004053155A1 (en) 2004-06-24
EP1570086B1 (de) 2013-02-20
CN105349657A (zh) 2016-02-24
JP4377378B2 (ja) 2009-12-02
EP1570079B1 (de) 2010-07-21
JP4377375B2 (ja) 2009-12-02
WO2004053457A2 (en) 2004-06-24
AU2003300825A8 (en) 2004-06-30
CA2507240C (en) 2011-05-24

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