WO2004024944A3 - Procede et sequences nucleotidiques pour la detection et l'identification de microorganismes dans un melange complexe ou dans l’eau - Google Patents

Procede et sequences nucleotidiques pour la detection et l'identification de microorganismes dans un melange complexe ou dans l’eau Download PDF

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Publication number
WO2004024944A3
WO2004024944A3 PCT/FR2003/002709 FR0302709W WO2004024944A3 WO 2004024944 A3 WO2004024944 A3 WO 2004024944A3 FR 0302709 W FR0302709 W FR 0302709W WO 2004024944 A3 WO2004024944 A3 WO 2004024944A3
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WO
WIPO (PCT)
Prior art keywords
water
complex mixture
detection
micro
identification
Prior art date
Application number
PCT/FR2003/002709
Other languages
English (en)
Other versions
WO2004024944A2 (fr
Inventor
Stephane Jean-Claude Bonilla
Sebastien Alain Christi Bouton
Nadine Batisse-Debitte
Gabriel Festoc
Original Assignee
Genesystems
Stephane Jean-Claude Bonilla
Sebastien Alain Christi Bouton
Nadine Batisse-Debitte
Gabriel Festoc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR0211400A external-priority patent/FR2844522A1/fr
Application filed by Genesystems, Stephane Jean-Claude Bonilla, Sebastien Alain Christi Bouton, Nadine Batisse-Debitte, Gabriel Festoc filed Critical Genesystems
Priority to AU2003282157A priority Critical patent/AU2003282157A1/en
Publication of WO2004024944A2 publication Critical patent/WO2004024944A2/fr
Publication of WO2004024944A3 publication Critical patent/WO2004024944A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L’invention concerne un procédé pour la détection, l’identification et, le cas échéant, la quantification des bactéries contenues dans un mélange complexe ou dans de l’eau, ledit procédé comprenant une étape d’amplifications multiples, simultanées mais séparées dans l’espace, de séquences d’acides nucléiques cibles spécifiques et exclusives. La présente invention a en outre pour objets des couples d’amorces pour amplification spécifiques et exclusifs, utiles à la mise en oeuvre dudit procédé, ainsi que des sondes nucléotidiques pour l’amplification en temps réel ou la détection de séquences d’acides nucléiques spécifiques et exclusives des bactéries contenues dans un mélange complexe ou dans de l’eau. L’invention concerne enfin des outis de biologie moléculaire, à savoir un kit d’analyse et des micro-réseaux ou puces à acides nucléiques pour la détection, l’identification et, le cas échéant, la quantification des bactéries contenues dans un mélange complexe ou dans de l’eau.
PCT/FR2003/002709 2002-09-13 2003-09-12 Procede et sequences nucleotidiques pour la detection et l'identification de microorganismes dans un melange complexe ou dans l’eau WO2004024944A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003282157A AU2003282157A1 (en) 2002-09-13 2003-09-12 Method and nucleotide sequences for the detection and identification of micro-organisms in a complex mixture or water

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR02/11400 2002-09-13
FR0211400A FR2844522A1 (fr) 2002-09-13 2002-09-13 Procede et sequences nucleotidiques pour la detection et l'identification de microorganismes dans un melange complexe
FR03/06905 2003-06-06
FR0306905A FR2844523B1 (fr) 2002-09-13 2003-06-06 Procede et sequences nucleotidiques pour la detection et l'identification de microorganismes dans un melange complexe ou dans de l'eau

Publications (2)

Publication Number Publication Date
WO2004024944A2 WO2004024944A2 (fr) 2004-03-25
WO2004024944A3 true WO2004024944A3 (fr) 2004-09-16

Family

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Application Number Title Priority Date Filing Date
PCT/FR2003/002709 WO2004024944A2 (fr) 2002-09-13 2003-09-12 Procede et sequences nucleotidiques pour la detection et l'identification de microorganismes dans un melange complexe ou dans l’eau

Country Status (3)

Country Link
AU (1) AU2003282157A1 (fr)
FR (1) FR2844523B1 (fr)
WO (1) WO2004024944A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8206923B2 (en) * 2006-04-24 2012-06-26 Elvira Garza Gonzalez Method for detection and multiple, simultaneous quantification of pathogens by means of real-time polymerase chain reaction
PT1862557E (pt) * 2006-05-29 2011-09-01 Bio Rad Pasteur Detecção múltipla em tempo real de três espécies bacterianas responsáveis por doenças sexualmente transmissíveis
EP2358908B1 (fr) 2008-11-14 2014-01-08 Gen-Probe Incorporated Compositions et procédés pour la détection d'acide nucléique de campylobacter
FR2970264B1 (fr) 2011-01-12 2014-09-05 Ct Scient Tech Batiment Cstb Procede et fragments de nucleotides pour la detection et/ou la quantification d'hotes de legionelles, kit associe

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002009877A1 (fr) * 2000-07-28 2002-02-07 Genesystems Dispositif pour l'amplification en châine thermo-dependante de sequences d'acides nucleiques cibles

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002009877A1 (fr) * 2000-07-28 2002-02-07 Genesystems Dispositif pour l'amplification en châine thermo-dependante de sequences d'acides nucleiques cibles

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
ASIM K BEJ ET AL: "MULTIPLEX PCR AMPLIFICATION AND IMMOBILIZED CAPTURE PROBES FOR DETECTION OF BACTERIAL PATHOGENES AND INDICATORS IN WATER", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 4, 1990, pages 353 - 365, XP000578307, ISSN: 0890-8508 *
BLOOD, vol. 100, no. 11, 16 November 2002 (2002-11-16), 44th Annual Meeting of the American Society of Hematology;Philadelphia, PA, USA; December 06-10, 2002, pages Abstract No. 1271, ISSN: 0006-4971 *
BLOOD, vol. 102, no. 11, 16 November 2003 (2003-11-16), 45th Annual Meeting of the American Society of Hematology;San Diego, CA, USA; December 06-09, 2003, pages 374a, ISSN: 0006-4971 *
BRASHER CYNTHIA W ET AL: "Detection of microbial pathogens in shellfish with multiplex PCR", CURRENT MICROBIOLOGY, vol. 37, no. 2, August 1998 (1998-08-01), pages 101 - 107, XP002277350, ISSN: 0343-8651 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 16 November 2002 (2002-11-16), BEILLARD EMMANUEL ET AL: "Innovative PCR Screening Assay with Biochip Detection for Revealing Fusion Gene Transcripts Involving the MLL Gene (n=30) in Acute Leukemias.", XP002278081, Database accession no. PREV200300335871 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 16 November 2003 (2003-11-16), HERMITTE FABIENNE ET AL: "Multi-center evaluation of comprehensive analysis of MLL translocation and positional breakpoint of fusion gene partners in Acute Lymphobastic and Myeloid Leukemia.", XP002278082, Database accession no. PREV200400133865 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; November 2001 (2001-11-01), SEN KEYA ET AL: "Multiplex PCR for detection of Enterobacteriaceae in blood", XP002277643, Database accession no. PREV200200056879 *
DATABASE EBI [online] STACKENBRANDT E., XP002242218, Database accession no. X65999 *
IBRAHIM A. ET AL.: "The polymerase chain reaction: an epidemiological tool to differentiate between two clusters of pathogenic Yersinia enterocolitica strains", FEMS MICROBIOL. LETT., vol. 97, 1992, pages 63 - 66, XP009011442 *
KONG R Y C ET AL: "Rapid detection of six types of bacterial pathogens in marine waters by multiplex PCR", WATER RESEARCH, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 36, no. 11, June 2002 (2002-06-01), pages 2802 - 2812, XP004361275, ISSN: 0043-1354 *
NOGVA HEGE KARIN ET AL: "Application of 5'-nuclease PCR for quantitative detection of Listeria monocytogenes in pure cultures, water, skim milk, and unpasteurized whole milk.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 10, October 2000 (2000-10-01), pages 4266 - 4271, XP002242217, ISSN: 0099-2240 *
TRANSFUSION (BETHESDA), vol. 41, no. 11, November 2001 (2001-11-01), pages 1356 - 1364, ISSN: 0041-1132 *
TSEN HAU-YANG ET AL: "Development of DNA probes and PCR primers for the specific detection of food pathogens.", FOOD SCIENCE AND AGRICULTURAL CHEMISTRY, vol. 3, no. 1, March 2001 (2001-03-01), pages 1 - 7, XP009011278, ISSN: 1560-4152 *
WESTIN LORELEI ET AL: "Antimicrobial resistance and bacterial identification utilizing a microelectronic chip array.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 39, no. 3, March 2001 (2001-03-01), pages 1097 - 1104, XP002242216, ISSN: 0095-1137 *

Also Published As

Publication number Publication date
AU2003282157A8 (en) 2004-04-30
WO2004024944A2 (fr) 2004-03-25
AU2003282157A1 (en) 2004-04-30
FR2844523A1 (fr) 2004-03-19
FR2844523B1 (fr) 2011-01-14

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