JP5213297B2 - 単一ラベルオリゴヌクレオチド・プローブ - Google Patents

単一ラベルオリゴヌクレオチド・プローブ Download PDF

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Publication number
JP5213297B2
JP5213297B2 JP2002519680A JP2002519680A JP5213297B2 JP 5213297 B2 JP5213297 B2 JP 5213297B2 JP 2002519680 A JP2002519680 A JP 2002519680A JP 2002519680 A JP2002519680 A JP 2002519680A JP 5213297 B2 JP5213297 B2 JP 5213297B2
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probe
nucleic acid
target nucleic
oligonucleotide
hybridization
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Japanese (ja)
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JP2004506431A (ja
JP2004506431A5 (https=
Inventor
ウィットワー,カール,ティー.
クロケット,アンドリュー,オー.
カプリン,ブライアン,イー.
スティーヴンソン,ウェイド
ワグナー,ロリ,エー.
チェン,ジャン
クスカワ,ノリコ
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UNIVERSITY OF UTHA RESEARCHFOUNDATION
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UNIVERSITY OF UTHA RESEARCHFOUNDATION
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
JP2002519680A 2000-08-11 2001-08-10 単一ラベルオリゴヌクレオチド・プローブ Expired - Lifetime JP5213297B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22472600P 2000-08-11 2000-08-11
US60/224,726 2000-08-11
US24061000P 2000-10-16 2000-10-16
US60/240,610 2000-10-16
PCT/US2001/025231 WO2002014555A2 (en) 2000-08-11 2001-08-10 Single-labeled oligonucleotide probes

Publications (3)

Publication Number Publication Date
JP2004506431A JP2004506431A (ja) 2004-03-04
JP2004506431A5 JP2004506431A5 (https=) 2008-10-09
JP5213297B2 true JP5213297B2 (ja) 2013-06-19

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JP2002519680A Expired - Lifetime JP5213297B2 (ja) 2000-08-11 2001-08-10 単一ラベルオリゴヌクレオチド・プローブ

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Country Link
US (1) US6635427B2 (https=)
EP (1) EP1307592B1 (https=)
JP (1) JP5213297B2 (https=)
AT (1) ATE332398T1 (https=)
CA (1) CA2417986C (https=)
DE (1) DE60121342T2 (https=)
ES (1) ES2267803T3 (https=)
WO (1) WO2002014555A2 (https=)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
EP1278889B1 (en) * 2000-03-29 2006-07-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US7998673B2 (en) * 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
CA2484215C (en) 2002-04-23 2015-02-24 Kirk Max Ririe Sample withdrawal and dispensing device
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7122384B2 (en) * 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
JP4377375B2 (ja) 2002-12-06 2009-12-02 エフ.ホフマン−ラ ロシュ アーゲー 病原性生物の多重分析検出
CA2525482C (en) 2003-04-04 2015-02-17 F. Hoffmann-La Roche Ag Improved system for multicolor real-time pcr comprising 3 to 6 pairs of forester resonance energy transfer (fret) probes
JP4454249B2 (ja) 2003-04-18 2010-04-21 アークレイ株式会社 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP1689764B1 (en) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Oligonucleotides labeled with a plurality of fluorophores
FR2866349B1 (fr) * 2004-02-12 2008-05-16 Biomerieux Sa Procede de diagnostic/pronostic d'une thrombose
US20080032413A1 (en) * 2004-04-12 2008-02-07 Byeang-Hyean Kim Oligonucleotide For Detecting Target Dna Or Rna
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
CA2594223C (en) * 2005-01-03 2016-03-15 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
EP1681555B1 (en) 2005-01-18 2007-03-21 Roche Diagnostics GmbH Imaging of fluorescence signals using telecentric excitation and imaging optics
EP1681556B1 (en) 2005-01-18 2007-04-11 Roche Diagnostics GmbH Imaging fluorescence signals using telecentricity
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
DE502005008153D1 (de) 2005-11-23 2009-10-29 Roche Diagnostics Gmbh Polynukleotid mit Phosphatmimetikum
US7759470B2 (en) * 2006-02-20 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
CN101421417B (zh) 2006-02-27 2013-04-03 霍夫曼-拉罗奇有限公司 经由镁螯合的pcr热启动
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
WO2008020090A1 (es) * 2006-08-18 2008-02-21 Laboratorios Indas, S.A. Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa
CN102851369B (zh) 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
EP2126114B1 (en) * 2007-02-01 2016-09-28 Abacus Diagnostica OY Method for detection of presence of target polynucleotide in samples
US20100068713A1 (en) * 2007-07-13 2010-03-18 Arkray, Inc. Probe for detecting mutation in jak2 gene and use thereof
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
AU2009262870B2 (en) * 2008-05-30 2014-03-20 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
WO2010018245A1 (es) * 2008-08-12 2010-02-18 Biotools Biotechnological & Medical Laboratories, S.A. Método para la detección y/o cuantificación de un ácido nucleico substrato
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
EP2436777B1 (en) 2009-05-26 2015-08-19 Xiamen University Method for detecting variations in nucleic acid sequences
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
US8748095B2 (en) * 2009-10-30 2014-06-10 Arkray, Inc. Probe for detecting polymorphism in EGFR gene and use of the probe
EP2502994A4 (en) * 2009-11-19 2013-05-22 Arkray Inc PRIMER SET FOR MTHFR GENE AMPLIFICATION, MTHFR GENE AMPLIFICATION REAGENT COMPRISING THE SAME, AND USE THEREOF
WO2011071046A1 (ja) * 2009-12-07 2011-06-16 アークレイ株式会社 疾患関連遺伝子の多型検出用プローブおよびその用途
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
JP5874152B2 (ja) * 2010-04-21 2016-03-02 国立研究開発法人理化学研究所 消光性ならびに蛍光性の核酸塩基類似体とその応用
JP5940771B2 (ja) 2010-07-12 2016-06-29 アークレイ株式会社 abl遺伝子多型の検出用プローブおよびその用途
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
EP2518165A1 (en) * 2011-04-28 2012-10-31 ARKRAY, Inc. Method for simultaneously detecting gene mutations of acetaldehyde dehydrogenase 2 and alcohol dehydrogenase 2
US10557170B2 (en) 2011-06-16 2020-02-11 Gendiag.Exe, S.L. Thromboembolic disease markers
EP2722388B1 (en) * 2011-06-16 2017-08-09 Nippon Steel & Sumikin Eco-Tech Corporation Nucleic acid probe for assaying nucleic acids
ES2547075T3 (es) 2011-06-16 2015-10-01 Gendiag.Exe, S.L. SNP asociados a enfermedad tromboembólica
WO2013034160A1 (en) * 2011-09-06 2013-03-14 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Methods for analyzing biological macromolecular complexes and use thereof
JP2013074888A (ja) * 2011-09-15 2013-04-25 Arkray Inc IL28B(rs8099917)とITPA(rs1127354)の変異を検出する方法
WO2013112458A1 (en) 2012-01-24 2013-08-01 Beth Israel Deaconess Medical Center, Inc. Novel chrebp isoforms and methods using the same
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP4570924A3 (en) * 2013-01-24 2025-09-03 California Institute of Technology Chromophore-based characterization and detection methods
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
WO2014189843A1 (en) 2013-05-20 2014-11-27 Board Of Trustees Of The University Of Arkansas Gep5 model for multiple myeloma
GB201319180D0 (en) 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
EP3189159A4 (en) 2014-06-26 2018-05-30 University of Florida Research Foundation, Inc. Rapid detection of infectious agents
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
CA2983819A1 (en) 2015-04-24 2016-10-27 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
US11434533B2 (en) 2016-02-09 2022-09-06 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid and nucleic acid probe used therein
MA45783A (fr) 2016-07-29 2019-06-05 Juno Therapeutics Inc Procédés d'évaluation de la présence ou de l'absence d'un virus compétent pour la réplication
JP2020526194A (ja) 2017-06-29 2020-08-31 ジュノー セラピューティクス インコーポレイテッド 免疫療法薬と関連する毒性を評価するためのマウスモデル
SG11202000606TA (en) 2017-07-29 2020-02-27 Juno Therapeutics Inc Reagents for expanding cells expressing recombinant receptors
WO2019046832A1 (en) 2017-09-01 2019-03-07 Juno Therapeutics, Inc. GENE EXPRESSION AND EVALUATION OF RISK OF DEVELOPMENT OF TOXICITY FOLLOWING CELL THERAPY
CN107727618B (zh) * 2017-09-04 2019-12-31 江苏大学 生物芯片和特异性切割位点dna序列检测汞离子的方法
EP3704229B1 (en) 2017-11-01 2023-12-20 Juno Therapeutics, Inc. Process for producing a t cell composition
JP2021511802A (ja) 2018-01-31 2021-05-13 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法および試薬
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
ES2968737T3 (es) 2018-11-06 2024-05-13 Juno Therapeutics Inc Proceso para producir células T manipuladas genéticamente
CA3120868A1 (en) 2018-11-30 2020-06-04 Alpine Immune Sciences, Inc. Cd86 variant immunomodulatory proteins and uses thereof
MX2021012607A (es) 2019-04-17 2022-03-11 Alpine Immune Sciences Inc Metodos y usos de proteinas de fusion de ligando icos (icosl) variante.
KR20220034782A (ko) 2019-06-12 2022-03-18 주노 쎄러퓨티크스 인코퍼레이티드 세포 매개 세포 독성 요법 및 친생존 bcl2 패밀리 단백질 억제제의 병용 요법
CN114555112A (zh) 2019-08-22 2022-05-27 朱诺治疗学股份有限公司 T细胞疗法和zeste增强子同源物2(ezh2)抑制剂的组合疗法及相关方法
EP4034674B1 (en) * 2019-09-23 2025-03-26 Universiteit Gent Probe and method for str-genotyping
KR102107589B1 (ko) * 2019-11-20 2020-05-07 주식회사 유진셀 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
AU2021209068B2 (en) 2020-01-13 2024-09-19 Aspen Neuroscience, Inc. Method of differentiating neural cells and related compositions and methods of use
CN115835873A (zh) 2020-05-13 2023-03-21 朱诺治疗学股份有限公司 用于产生表达重组受体的供体分批细胞的方法
JP2023531531A (ja) 2020-06-26 2023-07-24 ジュノ セラピューティクス ゲーエムベーハー 組換え受容体を条件付きで発現する操作されたt細胞、関連ポリヌクレオチド、および方法
US20240329032A1 (en) 2021-07-21 2024-10-03 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
AU2022402153A1 (en) 2021-12-03 2024-06-13 Medicinal Genomics Corporation Psilocybe assay
WO2023201361A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
EP4532695A1 (en) 2022-05-25 2025-04-09 Celgene Corporation Methods of manufacturing t cell therapies
AU2023343062A1 (en) 2022-09-13 2025-04-03 Medicinal Genomics Corporation Psilocybe assay
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
AU693023B2 (en) 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
GB9602025D0 (en) * 1996-02-01 1996-04-03 Amersham Int Plc Nucleoside analogues
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
AU733647B2 (en) 1996-05-15 2001-05-17 Biogenex Laboratories Non-nucleotide linking reagents
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Pcr中のハイブリダイゼーションのモニタリング
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
WO1998026093A2 (en) * 1996-12-13 1998-06-18 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
SE522077C2 (sv) 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
US6465175B2 (en) * 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
DE19858588B4 (de) 1998-08-22 2016-04-07 Qiagen Gmbh Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls
EP1046717B1 (en) 1999-04-20 2010-10-06 National Institute of Advanced Industrial Science and Technology Method and probes for determining a concentration of target nucleic acid molecules and method for analyzing data obtained by the method
SE9902565D0 (sv) 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
EP1278889B1 (en) * 2000-03-29 2006-07-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination

Also Published As

Publication number Publication date
DE60121342T2 (de) 2007-06-28
US6635427B2 (en) 2003-10-21
EP1307592A2 (en) 2003-05-07
WO2002014555A3 (en) 2003-02-27
CA2417986A1 (en) 2002-02-21
JP2004506431A (ja) 2004-03-04
EP1307592B1 (en) 2006-07-05
ATE332398T1 (de) 2006-07-15
CA2417986C (en) 2013-11-26
DE60121342D1 (de) 2006-08-17
ES2267803T3 (es) 2007-03-16
WO2002014555A2 (en) 2002-02-21
US20030022177A1 (en) 2003-01-30

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