ES2267803T3 - Sondas de oligonucleotidos marcados individuales. - Google Patents

Sondas de oligonucleotidos marcados individuales. Download PDF

Info

Publication number
ES2267803T3
ES2267803T3 ES01959722T ES01959722T ES2267803T3 ES 2267803 T3 ES2267803 T3 ES 2267803T3 ES 01959722 T ES01959722 T ES 01959722T ES 01959722 T ES01959722 T ES 01959722T ES 2267803 T3 ES2267803 T3 ES 2267803T3
Authority
ES
Spain
Prior art keywords
baselineskip
probe
dddseqskip
hskip
hfill
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES01959722T
Other languages
English (en)
Spanish (es)
Inventor
Carl T. Wittwer
Andrew O. Crockett
Brian E. Caplin
Wade Stevenson
Lori A. Wagner
Jian Chen
Noriko Kusukawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biofire Defense LLC
University of Utah Research Foundation Inc
Original Assignee
University of Utah Research Foundation Inc
Idaho Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Utah Research Foundation Inc, Idaho Technology Inc filed Critical University of Utah Research Foundation Inc
Application granted granted Critical
Publication of ES2267803T3 publication Critical patent/ES2267803T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
ES01959722T 2000-08-11 2001-08-10 Sondas de oligonucleotidos marcados individuales. Expired - Lifetime ES2267803T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US22472600P 2000-08-11 2000-08-11
US224726P 2000-08-11
US24061000P 2000-10-16 2000-10-16
US240610P 2000-10-16

Publications (1)

Publication Number Publication Date
ES2267803T3 true ES2267803T3 (es) 2007-03-16

Family

ID=26918967

Family Applications (1)

Application Number Title Priority Date Filing Date
ES01959722T Expired - Lifetime ES2267803T3 (es) 2000-08-11 2001-08-10 Sondas de oligonucleotidos marcados individuales.

Country Status (8)

Country Link
US (1) US6635427B2 (https=)
EP (1) EP1307592B1 (https=)
JP (1) JP5213297B2 (https=)
AT (1) ATE332398T1 (https=)
CA (1) CA2417986C (https=)
DE (1) DE60121342T2 (https=)
ES (1) ES2267803T3 (https=)
WO (1) WO2002014555A2 (https=)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
EP1715063B1 (en) 2000-03-29 2011-01-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US7998673B2 (en) 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US8409508B2 (en) 2002-04-23 2013-04-02 Biofire Diagnostics, Inc. Sample withdrawal and dispensing device
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7122384B2 (en) * 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
DE60333480D1 (de) 2002-12-06 2010-09-02 Roche Diagnostics Gmbh Verfahren zum nachweis von pathogenen organismen
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
ATE550099T1 (de) 2003-04-04 2012-04-15 Hoffmann La Roche Verbessertes system für mehrfarbige echtzeit-pcr
JP4454249B2 (ja) 2003-04-18 2010-04-21 アークレイ株式会社 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP1689764B1 (en) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Oligonucleotides labeled with a plurality of fluorophores
FR2866349B1 (fr) * 2004-02-12 2008-05-16 Biomerieux Sa Procede de diagnostic/pronostic d'une thrombose
US20080032413A1 (en) * 2004-04-12 2008-02-07 Byeang-Hyean Kim Oligonucleotide For Detecting Target Dna Or Rna
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
AU2006204087B2 (en) * 2005-01-03 2010-09-16 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
DE602005000877T2 (de) 2005-01-18 2007-12-20 Roche Diagnostics Gmbh Fluoreszenzabbildung mittels Telezentrizität
ATE357653T1 (de) 2005-01-18 2007-04-15 Hoffmann La Roche Fluoreszenzabbildung mittels telezentrischer anregungs- und abbildungsoptiken
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
EP1801114B8 (de) 2005-11-23 2009-12-02 Roche Diagnostics GmbH Polynukleotid mit Phosphatmimetikum
JP4713514B2 (ja) * 2006-02-20 2011-06-29 エフ．ホフマン−ラ ロシュ アーゲー 改善された標識試薬
ATE510027T1 (de) 2006-02-27 2011-06-15 Hoffmann La Roche Pcr-heissstart durch magnesium-sequestrierung
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
WO2008020090A1 (es) * 2006-08-18 2008-02-21 Laboratorios Indas, S.A. Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa
EP2857526B1 (en) 2006-12-13 2016-08-17 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
US8247171B2 (en) * 2007-02-01 2012-08-21 Abacus Diagnostica Oy Method for detection of presence of target polynucleotide in samples
JPWO2009011297A1 (ja) * 2007-07-13 2010-09-24 アークレイ株式会社 Ｊａｋ２遺伝子の変異検出用プローブおよびその用途
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
AU2009262870B2 (en) 2008-05-30 2014-03-20 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
WO2010018245A1 (es) * 2008-08-12 2010-02-18 Biotools Biotechnological & Medical Laboratories, S.A. Método para la detección y/o cuantificación de un ácido nucleico substrato
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
EP2436777B1 (en) 2009-05-26 2015-08-19 Xiamen University Method for detecting variations in nucleic acid sequences
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
WO2011052754A1 (ja) * 2009-10-30 2011-05-05 アークレイ株式会社 Ｅｇｆｒ遺伝子多型検出用プローブおよびその用途
WO2011062258A1 (ja) * 2009-11-19 2011-05-26 アークレイ株式会社 Ｍｔｈｆｒ遺伝子増幅用プライマーセット、それを含むｍｔｈｆｒ遺伝子増幅用試薬およびその用途
CN102317454A (zh) * 2009-12-07 2012-01-11 爱科来株式会社 疾病相关基因的多态性检测用探针及其用途
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
US9285319B2 (en) 2010-04-21 2016-03-15 Riken Nucleic acid base analogs with quenching and fluorescent activities and applications thereof
JP5940771B2 (ja) * 2010-07-12 2016-06-29 アークレイ株式会社 ａｂｌ遺伝子多型の検出用プローブおよびその用途
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
US20120276533A1 (en) * 2011-04-28 2012-11-01 Arkray, Inc. Method for Simultaneously Detecting Polymorphisms of Acetaldehyde Dehydrogenase 2 and Alcohol Dehydrogenase 2
ES2547075T3 (es) 2011-06-16 2015-10-01 Gendiag.Exe, S.L. SNP asociados a enfermedad tromboembólica
US10557170B2 (en) 2011-06-16 2020-02-11 Gendiag.Exe, S.L. Thromboembolic disease markers
US20140248611A1 (en) * 2011-06-16 2014-09-04 Nippon Steel & Sumikin Eco-Tech Corporation Nucleic acid probe for assaying nucleic acids
US9366677B2 (en) * 2011-09-06 2016-06-14 Max-Planck-Gesellshaft Zur Foerderung Der Wissenschaften E.V. Methods for analyzing biological macromolecular complexes and use thereof
JP2013074888A (ja) 2011-09-15 2013-04-25 Arkray Inc ＩＬ２８Ｂ（ｒｓ８０９９９１７）とＩＴＰＡ（ｒｓ１１２７３５４）の変異を検出する方法
US20150126438A1 (en) 2012-01-24 2015-05-07 Beth Israel Deaconess Medical Center, Inc. Novel ChREBP Isoforms and Methods Using the Same
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP2948566B1 (en) 2013-01-24 2025-03-26 California Institute of Technology Chromophore-based characterization and detection methods
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
WO2014189843A1 (en) 2013-05-20 2014-11-27 Board Of Trustees Of The University Of Arkansas Gep5 model for multiple myeloma
GB201319180D0 (en) * 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
WO2015200855A1 (en) 2014-06-26 2015-12-30 University Of Florida Research Foundation, Inc. Rapid detection of infectious agents
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
AU2016252998B2 (en) 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
CA3012815C (en) * 2016-02-09 2021-09-28 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid and nucleic acid probe used therein
JP7483373B2 (ja) 2016-07-29 2024-05-15 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法
EP3644721A1 (en) 2017-06-29 2020-05-06 Juno Therapeutics, Inc. Mouse model for assessing toxicities associated with immunotherapies
BR112020001719A2 (pt) 2017-07-29 2020-07-21 Juno Therapeutics Inc reagentes para expansão de células que expressam receptores recombinantes
WO2019046832A1 (en) 2017-09-01 2019-03-07 Juno Therapeutics, Inc. GENE EXPRESSION AND EVALUATION OF RISK OF DEVELOPMENT OF TOXICITY FOLLOWING CELL THERAPY
CN107727618B (zh) * 2017-09-04 2019-12-31 江苏大学 生物芯片和特异性切割位点dna序列检测汞离子的方法
SG11202003657VA (en) 2017-11-01 2020-05-28 Juno Therapeutics Inc Process for producing a t cell composition
US11535903B2 (en) 2018-01-31 2022-12-27 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
EP3877054B1 (en) 2018-11-06 2023-11-01 Juno Therapeutics, Inc. Process for producing genetically engineered t cells
WO2020113141A2 (en) 2018-11-30 2020-06-04 Alpine Immune Sciences, Inc. Cd86 variant immunomodulatory proteins and uses thereof
EP4438054A3 (en) 2019-04-17 2025-01-08 Alpine Immune Sciences, Inc. Methods and uses of variant icos ligand (icosl) fusion proteins
EP3983006A1 (en) 2019-06-12 2022-04-20 Juno Therapeutics, Inc. Combination therapy of a cell-mediated cytotoxic therapy and an inhibitor of a prosurvival bcl2 family protein
JP2022545467A (ja) 2019-08-22 2022-10-27 ジュノー セラピューティクス インコーポレイテッド Ｔ細胞療法とｚｅｓｔｅホモログ２エンハンサー（ＥＺＨ２）阻害剤との併用療法および関連方法
US20220340981A1 (en) * 2019-09-23 2022-10-27 Universiteit Gent Probe and method for str-genotyping
KR102107589B1 (ko) * 2019-11-20 2020-05-07 주식회사 유진셀 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
MX2022008625A (es) 2020-01-13 2022-09-09 Aspen Neuroscience Inc Metodo de diferenciacion de celulas neuronales y composiciones relacionadas y metodos de uso.
WO2021231661A2 (en) 2020-05-13 2021-11-18 Juno Therapeutics, Inc. Process for producing donor-batched cells expressing a recombinant receptor
EP4171616A1 (en) 2020-06-26 2023-05-03 Juno Therapeutics GmbH Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
US20240329032A1 (en) 2021-07-21 2024-10-03 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
CA3241326A1 (en) 2021-12-03 2023-06-08 Medicinal Genomics Corporation Psilocybe assay
EP4508645A1 (en) 2022-04-15 2025-02-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
EP4532695A1 (en) 2022-05-25 2025-04-09 Celgene Corporation Methods of manufacturing t cell therapies
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
EP0826066B1 (en) 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
GB9602025D0 (en) * 1996-02-01 1996-04-03 Amersham Int Plc Nucleoside analogues
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
CA2253969A1 (en) 1996-05-15 1997-11-20 Rajkumar Siva Iyer Non-nucleotide linking reagents
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
US6451530B1 (en) * 1996-12-13 2002-09-17 The United States Of America As Represented By The Department Of Health And Human Services Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
SE522077C2 (sv) * 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
WO1999011813A2 (en) * 1997-09-04 1999-03-11 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
DE19858588B4 (de) 1998-08-22 2016-04-07 Qiagen Gmbh Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls
CA2304260C (en) 1999-04-20 2009-03-24 Japan Bioindustry Association Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
SE9902565D0 (sv) * 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
EP1715063B1 (en) 2000-03-29 2011-01-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination

Also Published As

Publication number Publication date
DE60121342D1 (de) 2006-08-17
DE60121342T2 (de) 2007-06-28
CA2417986C (en) 2013-11-26
US6635427B2 (en) 2003-10-21
WO2002014555A2 (en) 2002-02-21
ATE332398T1 (de) 2006-07-15
EP1307592B1 (en) 2006-07-05
EP1307592A2 (en) 2003-05-07
JP5213297B2 (ja) 2013-06-19
WO2002014555A3 (en) 2003-02-27
JP2004506431A (ja) 2004-03-04
CA2417986A1 (en) 2002-02-21
US20030022177A1 (en) 2003-01-30

Similar Documents

Publication Publication Date Title
ES2267803T3 (es) Sondas de oligonucleotidos marcados individuales.
ES2205572T3 (es) Construcciones de cebador pcr para su utilizacion en un sistema integrado de señalizacion.
JP7050848B2 (ja) リコンビナーゼポリメラーゼ増幅を多重化するための方法
JP5385973B2 (ja) 反応における複数の核酸配列の同時検出
CA2321185C (en) Method for detection of target nucleic acids using pcr
AU2007306019B2 (en) Double-stranded probes for the fluorescent detection of nucleic acids
JP2006525027A (ja) 分子スイッチを含むオリゴヌクレオチド
CN101680029A (zh) 核酸检测
CN102762744A (zh) 利用靶信号产生引物进行的靶检测
ES2813899T3 (es) Ensayo de detección de diana multiplex
ES2776427T3 (es) Método de amplificación de ADN basado en invasión de cadena
ES2256067T3 (es) Oligonucleotidos y combinaciones de los mismos utiles en la deteccion de la presencia o ausencia de secuencias de acidos nucleicos diana en una muestra.
US7504218B2 (en) Key probe compositions and methods for polynucleotide detection
Belousov et al. Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse™ Probe System in challenging sequence environment
Harvey et al. Characterization and applications of CataCleave probe in real-time detection assays
ES2980651T3 (es) Método de detección de una secuencia de ácido nucleico
US20060194222A1 (en) Triplex probe compositions and methods for polynucleotide detection
Nurmi et al. High-throughput genetic analysis using time-resolved fluorometry and closed-tube detection
Vaughn et al. Hybridization-induced dequenching of fluorescein-labeled oligonucleotides: a novel strategy for PCR detection and genotyping
JP2008539761A (ja) スナップバックオリゴヌクレオチドプローブ
ES2245315T3 (es) Metodo de amplificacion para la deteccion de acidos nucleicos diana que implica transferencia de energia fluorescente.
ES3023217T3 (en) Probe and method for str-genotyping
JP2010536343A (ja) 薬剤耐性菌検出方法
JP6999645B2 (ja) 核酸の増幅及び検出／定量の効率を改良するためのヘルパーオリゴヌクレオチド
CA2705852C (en) Photoinduced electron transfer (pet) primer for nucleic acid amplification