DE60033381T2 - Verwendung von dreidimensionalem stromalen gewebe zur behandlung von ischemischen gewebe - Google Patents
Verwendung von dreidimensionalem stromalen gewebe zur behandlung von ischemischen gewebe Download PDFInfo
- Publication number
- DE60033381T2 DE60033381T2 DE60033381T DE60033381T DE60033381T2 DE 60033381 T2 DE60033381 T2 DE 60033381T2 DE 60033381 T DE60033381 T DE 60033381T DE 60033381 T DE60033381 T DE 60033381T DE 60033381 T2 DE60033381 T2 DE 60033381T2
- Authority
- DE
- Germany
- Prior art keywords
- cells
- tissue
- stromal
- use according
- dimensional
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000000302 ischemic effect Effects 0.000 title claims description 30
- 238000011282 treatment Methods 0.000 title description 18
- 210000001519 tissue Anatomy 0.000 claims abstract description 192
- 210000002216 heart Anatomy 0.000 claims abstract description 54
- 230000033115 angiogenesis Effects 0.000 claims abstract description 31
- 210000004165 myocardium Anatomy 0.000 claims abstract description 14
- 230000001737 promoting effect Effects 0.000 claims abstract description 12
- 210000002808 connective tissue Anatomy 0.000 claims abstract description 11
- 210000002027 skeletal muscle Anatomy 0.000 claims abstract description 7
- 210000002460 smooth muscle Anatomy 0.000 claims abstract description 7
- 210000004027 cell Anatomy 0.000 claims description 92
- 210000002536 stromal cell Anatomy 0.000 claims description 51
- 210000002950 fibroblast Anatomy 0.000 claims description 29
- 210000002889 endothelial cell Anatomy 0.000 claims description 26
- 210000005003 heart tissue Anatomy 0.000 claims description 25
- 108090000623 proteins and genes Proteins 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 21
- 108010035532 Collagen Proteins 0.000 claims description 16
- 102000008186 Collagen Human genes 0.000 claims description 16
- 229920001436 collagen Polymers 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 12
- 230000002107 myocardial effect Effects 0.000 claims description 9
- 210000000329 smooth muscle myocyte Anatomy 0.000 claims description 9
- 239000000990 laser dye Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- -1 polypropylene Polymers 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 7
- 230000001413 cellular effect Effects 0.000 claims description 6
- 239000000017 hydrogel Substances 0.000 claims description 6
- 210000004509 vascular smooth muscle cell Anatomy 0.000 claims description 6
- 239000004793 Polystyrene Substances 0.000 claims description 5
- 239000003364 biologic glue Substances 0.000 claims description 5
- 229920002223 polystyrene Polymers 0.000 claims description 5
- 210000003491 skin Anatomy 0.000 claims description 5
- 229920000742 Cotton Polymers 0.000 claims description 4
- 108010010803 Gelatin Proteins 0.000 claims description 4
- 229920000954 Polyglycolide Polymers 0.000 claims description 4
- 210000001789 adipocyte Anatomy 0.000 claims description 4
- 229920000159 gelatin Polymers 0.000 claims description 4
- 239000008273 gelatin Substances 0.000 claims description 4
- 235000019322 gelatine Nutrition 0.000 claims description 4
- 235000011852 gelatine desserts Nutrition 0.000 claims description 4
- 210000002540 macrophage Anatomy 0.000 claims description 4
- 210000001616 monocyte Anatomy 0.000 claims description 4
- 210000003668 pericyte Anatomy 0.000 claims description 4
- 229920000058 polyacrylate Polymers 0.000 claims description 4
- 239000004633 polyglycolic acid Substances 0.000 claims description 4
- 239000004826 Synthetic adhesive Substances 0.000 claims description 3
- 239000002729 catgut Substances 0.000 claims description 3
- 210000001174 endocardium Anatomy 0.000 claims description 3
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 229920002554 vinyl polymer Polymers 0.000 claims description 3
- 239000000020 Nitrocellulose Substances 0.000 claims description 2
- 239000004952 Polyamide Substances 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920001220 nitrocellulos Polymers 0.000 claims description 2
- 229920002647 polyamide Polymers 0.000 claims description 2
- 229920000728 polyester Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 210000003630 histaminocyte Anatomy 0.000 claims 2
- 210000000265 leukocyte Anatomy 0.000 claims 2
- 210000000663 muscle cell Anatomy 0.000 claims 2
- 210000004180 plasmocyte Anatomy 0.000 claims 2
- 239000003292 glue Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 23
- 210000000056 organ Anatomy 0.000 abstract description 11
- 238000002513 implantation Methods 0.000 abstract description 9
- 230000008439 repair process Effects 0.000 abstract description 9
- 230000007998 vessel formation Effects 0.000 abstract description 9
- 230000003872 anastomosis Effects 0.000 abstract description 8
- 230000035876 healing Effects 0.000 abstract description 7
- 230000008929 regeneration Effects 0.000 abstract description 5
- 238000011069 regeneration method Methods 0.000 abstract description 5
- 238000007675 cardiac surgery Methods 0.000 abstract 1
- 210000004204 blood vessel Anatomy 0.000 description 36
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 23
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 23
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 23
- 241001465754 Metazoa Species 0.000 description 22
- 230000015572 biosynthetic process Effects 0.000 description 20
- 239000000047 product Substances 0.000 description 17
- 239000003102 growth factor Substances 0.000 description 16
- 238000001356 surgical procedure Methods 0.000 description 16
- 239000007943 implant Substances 0.000 description 15
- 239000002609 medium Substances 0.000 description 15
- 230000002491 angiogenic effect Effects 0.000 description 14
- 230000017531 blood circulation Effects 0.000 description 14
- 241000699670 Mus sp. Species 0.000 description 13
- 208000027418 Wounds and injury Diseases 0.000 description 13
- 238000003556 assay Methods 0.000 description 13
- 230000035755 proliferation Effects 0.000 description 13
- 239000004744 fabric Substances 0.000 description 11
- 238000001727 in vivo Methods 0.000 description 11
- 239000002870 angiogenesis inducing agent Substances 0.000 description 10
- 230000006870 function Effects 0.000 description 10
- 239000012528 membrane Substances 0.000 description 10
- 206010052428 Wound Diseases 0.000 description 9
- 210000003711 chorioallantoic membrane Anatomy 0.000 description 9
- 230000006698 induction Effects 0.000 description 9
- 102000006495 integrins Human genes 0.000 description 9
- 108010044426 integrins Proteins 0.000 description 9
- 210000004379 membrane Anatomy 0.000 description 9
- 241000282887 Suidae Species 0.000 description 8
- 230000006907 apoptotic process Effects 0.000 description 8
- 108010082117 matrigel Proteins 0.000 description 8
- 229920001778 nylon Polymers 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 239000012981 Hank's balanced salt solution Substances 0.000 description 7
- 239000004677 Nylon Substances 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 210000001367 artery Anatomy 0.000 description 7
- 230000001143 conditioned effect Effects 0.000 description 7
- 208000010125 myocardial infarction Diseases 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 230000000638 stimulation Effects 0.000 description 7
- 230000002861 ventricular Effects 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 108010080379 Fibrin Tissue Adhesive Proteins 0.000 description 6
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 6
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 210000004351 coronary vessel Anatomy 0.000 description 6
- 108020004999 messenger RNA Proteins 0.000 description 6
- 239000002356 single layer Substances 0.000 description 6
- 208000001778 Coronary Occlusion Diseases 0.000 description 5
- 206010011086 Coronary artery occlusion Diseases 0.000 description 5
- 208000008960 Diabetic foot Diseases 0.000 description 5
- 206010061218 Inflammation Diseases 0.000 description 5
- 241000700159 Rattus Species 0.000 description 5
- 239000003636 conditioned culture medium Substances 0.000 description 5
- 230000006378 damage Effects 0.000 description 5
- 230000003292 diminished effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 230000004054 inflammatory process Effects 0.000 description 5
- 239000010410 layer Substances 0.000 description 5
- 230000004807 localization Effects 0.000 description 5
- 230000007774 longterm Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 210000003462 vein Anatomy 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 4
- 210000000709 aorta Anatomy 0.000 description 4
- 210000002565 arteriole Anatomy 0.000 description 4
- 230000001174 ascending effect Effects 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 4
- 230000012292 cell migration Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 230000010002 chemokinesis Effects 0.000 description 4
- 230000035605 chemotaxis Effects 0.000 description 4
- 230000001419 dependent effect Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000012091 fetal bovine serum Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- MOFVSTNWEDAEEK-UHFFFAOYSA-M indocyanine green Chemical compound [Na+].[O-]S(=O)(=O)CCCCN1C2=CC=C3C=CC=CC3=C2C(C)(C)C1=CC=CC=CC=CC1=[N+](CCCCS([O-])(=O)=O)C2=CC=C(C=CC=C3)C3=C2C1(C)C MOFVSTNWEDAEEK-UHFFFAOYSA-M 0.000 description 4
- 229960004657 indocyanine green Drugs 0.000 description 4
- 208000014674 injury Diseases 0.000 description 4
- 230000006654 negative regulation of apoptotic process Effects 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical class CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 3
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 3
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 3
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 3
- 238000011579 SCID mouse model Methods 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 3
- 102000004887 Transforming Growth Factor beta Human genes 0.000 description 3
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 238000001574 biopsy Methods 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 229940126864 fibroblast growth factor Drugs 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000001415 gene therapy Methods 0.000 description 3
- 208000019622 heart disease Diseases 0.000 description 3
- 210000003709 heart valve Anatomy 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 230000003472 neutralizing effect Effects 0.000 description 3
- 230000010412 perfusion Effects 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000012552 review Methods 0.000 description 3
- 239000000565 sealant Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- 239000003106 tissue adhesive Substances 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 229960001322 trypsin Drugs 0.000 description 3
- 210000005239 tubule Anatomy 0.000 description 3
- CQVWXNBVRLKXPE-UHFFFAOYSA-N 2-octyl cyanoacrylate Chemical compound CCCCCCC(C)OC(=O)C(=C)C#N CQVWXNBVRLKXPE-UHFFFAOYSA-N 0.000 description 2
- 206010053567 Coagulopathies Diseases 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 2
- 229920004934 Dacron® Polymers 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 102000016942 Elastin Human genes 0.000 description 2
- 108010014258 Elastin Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010049003 Fibrinogen Proteins 0.000 description 2
- 102000008946 Fibrinogen Human genes 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000011888 autopsy Methods 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 230000009087 cell motility Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 210000000038 chest Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000035602 clotting Effects 0.000 description 2
- 239000000512 collagen gel Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 102000038379 digestive enzymes Human genes 0.000 description 2
- 108091007734 digestive enzymes Proteins 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 231100000673 dose–response relationship Toxicity 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 229920002549 elastin Polymers 0.000 description 2
- 238000002565 electrocardiography Methods 0.000 description 2
- 230000003511 endothelial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 229940012952 fibrinogen Drugs 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 208000037906 ischaemic injury Diseases 0.000 description 2
- 208000028867 ischemia Diseases 0.000 description 2
- 210000005240 left ventricle Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 210000004925 microvascular endothelial cell Anatomy 0.000 description 2
- 210000004088 microvessel Anatomy 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 238000004264 monolayer culture Methods 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 2
- 210000003516 pericardium Anatomy 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- 229920000915 polyvinyl chloride Polymers 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000718 qrs complex Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000007634 remodeling Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000001177 retroviral effect Effects 0.000 description 2
- 239000012679 serum free medium Substances 0.000 description 2
- 210000004927 skin cell Anatomy 0.000 description 2
- 210000001626 skin fibroblast Anatomy 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 210000003556 vascular endothelial cell Anatomy 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- NALREUIWICQLPS-UHFFFAOYSA-N 7-imino-n,n-dimethylphenothiazin-3-amine;hydrochloride Chemical compound [Cl-].C1=C(N)C=C2SC3=CC(=[N+](C)C)C=CC3=NC2=C1 NALREUIWICQLPS-UHFFFAOYSA-N 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000000844 Cell Surface Receptors Human genes 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 102000019034 Chemokines Human genes 0.000 description 1
- 108010012236 Chemokines Proteins 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 206010010099 Combined immunodeficiency Diseases 0.000 description 1
- 229920001651 Cyanoacrylate Polymers 0.000 description 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 1
- 101710096438 DNA-binding protein Proteins 0.000 description 1
- 229920000045 Dermatan sulfate Polymers 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 206010056340 Diabetic ulcer Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 1
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 208000003790 Foot Ulcer Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 108060003393 Granulin Proteins 0.000 description 1
- 206010019273 Heart disease congenital Diseases 0.000 description 1
- 229920002971 Heparan sulfate Polymers 0.000 description 1
- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000001974 Hyaluronidases Human genes 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010061216 Infarction Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 229920000288 Keratan sulfate Polymers 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 102000007330 LDL Lipoproteins Human genes 0.000 description 1
- 108010007622 LDL Lipoproteins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 206010028851 Necrosis Diseases 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 229920002201 Oxidized cellulose Polymers 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 108010059712 Pronase Proteins 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 108091027981 Response element Proteins 0.000 description 1
- 108010081750 Reticulin Proteins 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 108010017842 Telomerase Proteins 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 102000006601 Thymidine Kinase Human genes 0.000 description 1
- 108020004440 Thymidine kinase Proteins 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000002399 angioplasty Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 230000015624 blood vessel development Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
- 210000001715 carotid artery Anatomy 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000001516 cell proliferation assay Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229940094517 chondroitin 4-sulfate Drugs 0.000 description 1
- KXKPYJOVDUMHGS-OSRGNVMNSA-N chondroitin sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](OS(O)(=O)=O)[C@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](C(O)=O)O1 KXKPYJOVDUMHGS-OSRGNVMNSA-N 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000515 collagen sponge Substances 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 230000030944 contact inhibition Effects 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- AVJBPWGFOQAPRH-FWMKGIEWSA-L dermatan sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@H](OS([O-])(=O)=O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O1 AVJBPWGFOQAPRH-FWMKGIEWSA-L 0.000 description 1
- 229940051593 dermatan sulfate Drugs 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 108010007093 dispase Proteins 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000002592 echocardiography Methods 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000013171 endarterectomy Methods 0.000 description 1
- 230000004528 endothelial cell apoptotic process Effects 0.000 description 1
- 230000026721 endothelial cell chemotaxis Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000002695 general anesthesia Methods 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036074 healthy skin Effects 0.000 description 1
- 230000002439 hemostatic effect Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000007574 infarction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000003601 intercostal effect Effects 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- YWXYYJSYQOXTPL-SLPGGIOYSA-N isosorbide mononitrate Chemical compound [O-][N+](=O)O[C@@H]1CO[C@@H]2[C@@H](O)CO[C@@H]21 YWXYYJSYQOXTPL-SLPGGIOYSA-N 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- KXCLCNHUUKTANI-RBIYJLQWSA-N keratan Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@H](COS(O)(=O)=O)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H]([C@@H](COS(O)(=O)=O)O[C@@H](O)[C@@H]3O)O)[C@H](NC(C)=O)[C@H]2O)COS(O)(=O)=O)O[C@H](COS(O)(=O)=O)[C@@H]1O KXCLCNHUUKTANI-RBIYJLQWSA-N 0.000 description 1
- 108010057670 laminin 1 Proteins 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 210000005246 left atrium Anatomy 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229940107304 oxidized cellulose Drugs 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 210000002976 pectoralis muscle Anatomy 0.000 description 1
- 229960001412 pentobarbital Drugs 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 229920002463 poly(p-dioxanone) polymer Polymers 0.000 description 1
- 229920002627 poly(phosphazenes) Polymers 0.000 description 1
- 239000000622 polydioxanone Substances 0.000 description 1
- 239000004626 polylactic acid Substances 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 210000003240 portal vein Anatomy 0.000 description 1
- 230000030503 positive regulation of chemotaxis Effects 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009993 protective function Effects 0.000 description 1
- 210000001147 pulmonary artery Anatomy 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229930002330 retinoic acid Natural products 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000002363 skeletal muscle cell Anatomy 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000013222 sprague-dawley male rat Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 210000000779 thoracic wall Anatomy 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 230000007838 tissue remodeling Effects 0.000 description 1
- 238000002627 tracheal intubation Methods 0.000 description 1
- 108091012338 transcription factor binding proteins Proteins 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 229960001727 tretinoin Drugs 0.000 description 1
- YFDSDPIBEUFTMI-UHFFFAOYSA-N tribromoethanol Chemical compound OCC(Br)(Br)Br YFDSDPIBEUFTMI-UHFFFAOYSA-N 0.000 description 1
- 229950004616 tribromoethanol Drugs 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 108010052768 tyrosyl-isoleucyl-glycyl-seryl-arginine Proteins 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000003954 umbilical cord Anatomy 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 230000006711 vascular endothelial growth factor production Effects 0.000 description 1
- 230000006444 vascular growth Effects 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 210000001631 vena cava inferior Anatomy 0.000 description 1
- 210000002620 vena cava superior Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3826—Muscle cells, e.g. smooth muscle cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/20—Materials or treatment for tissue regeneration for reconstruction of the heart, e.g. heart valves
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Transplantation (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Zoology (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Vascular Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Surgery (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12883899P | 1999-04-12 | 1999-04-12 | |
| US128838P | 1999-04-12 | ||
| US411585 | 1999-10-01 | ||
| US09/411,585 US20030007954A1 (en) | 1999-04-12 | 1999-10-01 | Methods for using a three-dimensional stromal tissue to promote angiogenesis |
| PCT/US2000/009848 WO2000061204A1 (en) | 1999-04-12 | 2000-04-12 | Three-dimensional stromal tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60033381D1 DE60033381D1 (de) | 2007-03-29 |
| DE60033381T2 true DE60033381T2 (de) | 2007-11-29 |
Family
ID=26826992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60033381T Expired - Lifetime DE60033381T2 (de) | 1999-04-12 | 2000-04-12 | Verwendung von dreidimensionalem stromalen gewebe zur behandlung von ischemischen gewebe |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US20030007954A1 (enExample) |
| EP (1) | EP1169069B1 (enExample) |
| JP (1) | JP4709393B2 (enExample) |
| AT (1) | ATE353676T1 (enExample) |
| AU (1) | AU777853C (enExample) |
| CA (1) | CA2367507C (enExample) |
| DE (1) | DE60033381T2 (enExample) |
| ES (1) | ES2282105T3 (enExample) |
| IL (2) | IL145892A0 (enExample) |
| WO (1) | WO2000061204A1 (enExample) |
Families Citing this family (96)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030007954A1 (en) * | 1999-04-12 | 2003-01-09 | Gail K. Naughton | Methods for using a three-dimensional stromal tissue to promote angiogenesis |
| US6585716B2 (en) * | 2000-04-05 | 2003-07-01 | Biocardia, Inc. | Method of treating the heart |
| EP1274471B1 (de) | 2000-04-11 | 2007-01-03 | Polyzenix GmbH | Verwendung von Folien aus Poly-Tri-Fluor-Ethoxypolyphosphazenen zur Umhüllung von medizinischen Vorrichtungen |
| US20050095268A1 (en) * | 2000-06-12 | 2005-05-05 | Acorn Cardiovascular, Inc. | Cardiac wall tension relief with cell loss management |
| US7326570B2 (en) * | 2000-06-16 | 2008-02-05 | The Regents Of The University Of California | Induction of tubular morphogenesis using pleiotrophin |
| US20080152629A1 (en) * | 2000-12-06 | 2008-06-26 | James Edinger | Placental stem cell populations |
| US9080146B2 (en) | 2001-01-11 | 2015-07-14 | Celonova Biosciences, Inc. | Substrates containing polyphosphazene as matrices and substrates containing polyphosphazene with a micro-structured surface |
| DE10100961B4 (de) | 2001-01-11 | 2005-08-04 | Polyzenix Gmbh | Körperverträglicher Werkstoff und mit diesem Werkstoff beschichtetes Substrat für die Züchtung von Zellen und künstlichen aus Zellen aufgebauten oder gewachsenen organischen Implantaten |
| WO2003025167A2 (en) * | 2001-09-20 | 2003-03-27 | Centre For Translational Research In Cancer | Cultured stromal cells and uses thereof |
| KR101042448B1 (ko) * | 2002-11-26 | 2011-06-16 | 안트로제네시스 코포레이션 | 세포요법제, 세포요법제 단위 및 이를 이용한 치료방법 |
| US8940292B2 (en) * | 2003-01-28 | 2015-01-27 | Wake Forest University Health Sciences | Enhancement of angiogenesis to grafts using cells engineered to produce growth factors |
| CN1770976A (zh) * | 2003-02-13 | 2006-05-10 | 人类起源公司 | 利用脐带血治疗患有疾病、紊乱或状况的个体的用途 |
| ES2564044T3 (es) | 2003-06-27 | 2016-03-17 | DePuy Synthes Products, Inc. | Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas |
| US9592258B2 (en) | 2003-06-27 | 2017-03-14 | DePuy Synthes Products, Inc. | Treatment of neurological injury by administration of human umbilical cord tissue-derived cells |
| AU2005322060B2 (en) | 2003-06-27 | 2011-11-17 | Ethicon Incorporated | Postpartum cells derived from umbilical cord tissue, and methods of making and using the same |
| US8491883B2 (en) * | 2003-06-27 | 2013-07-23 | Advanced Technologies And Regenerative Medicine, Llc | Treatment of amyotrophic lateral sclerosis using umbilical derived cells |
| US7875272B2 (en) | 2003-06-27 | 2011-01-25 | Ethicon, Incorporated | Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells |
| US9572840B2 (en) | 2003-06-27 | 2017-02-21 | DePuy Synthes Products, Inc. | Regeneration and repair of neural tissue using postpartum-derived cells |
| US8790637B2 (en) * | 2003-06-27 | 2014-07-29 | DePuy Synthes Products, LLC | Repair and regeneration of ocular tissue using postpartum-derived cells |
| US8518390B2 (en) | 2003-06-27 | 2013-08-27 | Advanced Technologies And Regenerative Medicine, Llc | Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells |
| NZ548049A (en) * | 2003-12-02 | 2009-01-31 | Celgene Corp | Methods and compositions for the treatment and management of hemoglobinopathy and anemia |
| US8486438B2 (en) * | 2004-01-29 | 2013-07-16 | Brown University | Methods for progenitor cell recruitment and isolation |
| WO2005093047A2 (en) * | 2004-03-24 | 2005-10-06 | Universite De Geneve | 3d-cardiac tissue engineering for the cell therapy of heart failure |
| WO2006026617A2 (en) * | 2004-08-30 | 2006-03-09 | Iken Tissue Therapeutics, Inc. | Compositions and methods of promoting hair growth |
| WO2006026730A2 (en) * | 2004-08-30 | 2006-03-09 | Iken Tissue Therapeutics, Inc. | Cultured three dimensional tissues and uses thereof |
| US9107850B2 (en) | 2004-10-25 | 2015-08-18 | Celonova Biosciences, Inc. | Color-coded and sized loadable polymeric particles for therapeutic and/or diagnostic applications and methods of preparing and using the same |
| US9114162B2 (en) | 2004-10-25 | 2015-08-25 | Celonova Biosciences, Inc. | Loadable polymeric particles for enhanced imaging in clinical applications and methods of preparing and using the same |
| US20210299056A9 (en) | 2004-10-25 | 2021-09-30 | Varian Medical Systems, Inc. | Color-Coded Polymeric Particles of Predetermined Size for Therapeutic and/or Diagnostic Applications and Related Methods |
| ATE500855T1 (de) * | 2004-11-22 | 2011-03-15 | Theregen Inc | Vewendung von dreidimensionalem kultiviertem gewebe zur behandlung von kongestivem herzversagen |
| US20060153815A1 (en) * | 2004-12-21 | 2006-07-13 | Agnieszka Seyda | Tissue engineering devices for the repair and regeneration of tissue |
| US20060171930A1 (en) * | 2004-12-21 | 2006-08-03 | Agnieszka Seyda | Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same |
| WO2006071802A2 (en) | 2004-12-23 | 2006-07-06 | Ethicon Incorporated | Treatment of stroke and other acute neural degenerative disorders using postpartum derived cells |
| CA2612188A1 (en) * | 2005-06-17 | 2007-01-04 | Theregen, Inc. | Methods for treating ischemic tissue |
| WO2007011644A2 (en) * | 2005-07-15 | 2007-01-25 | Cormatrix Cardiovascular, Inc. | Compositions for regenerating defective or absent tissue |
| DE102005045671A1 (de) * | 2005-09-15 | 2007-03-29 | Ossacur Ag | Verwendung eines Kollagens xenogenen Ursprungs |
| CA2624925C (en) | 2005-10-13 | 2014-09-30 | Anthrogenesis Corporation | Immunomodulation using placental stem cells |
| CN101374946B (zh) * | 2005-12-16 | 2017-07-18 | 伊西康公司 | 用于在组织相容性不匹配的移植中抑制有害的免疫反应的组合物和方法 |
| AU2006327073B2 (en) * | 2005-12-19 | 2012-08-30 | Ethicon, Inc. | In vitro expansion of postpartum derived cells in roller bottles |
| US9125906B2 (en) * | 2005-12-28 | 2015-09-08 | DePuy Synthes Products, Inc. | Treatment of peripheral vascular disease using umbilical cord tissue-derived cells |
| SG170789A1 (en) * | 2005-12-28 | 2011-05-30 | Ethicon Inc | Treatment of peripheral vascular disease using postpartum-derived cells |
| ES2708933T3 (es) | 2005-12-29 | 2019-04-12 | Celularity Inc | Poblaciones de células madre placentarias |
| WO2007092902A2 (en) * | 2006-02-07 | 2007-08-16 | Organogenesis, Inc. | Bioengineered tissue constructs and cardiac uses thereof |
| PT2089071E (pt) | 2006-10-10 | 2012-03-09 | Celonova Biosciences Inc | Válvula cardíaca bioprotética com polifosfazeno |
| CN101573442A (zh) * | 2006-11-09 | 2009-11-04 | J·大卫格莱斯顿学会 | 诱导心肌细胞形成的方法 |
| KR101569168B1 (ko) | 2007-02-12 | 2015-11-13 | 안트로제네시스 코포레이션 | 태반 줄기세포를 이용한 염증 질환의 치료 |
| US20100172830A1 (en) * | 2007-03-29 | 2010-07-08 | Cellx Inc. | Extraembryonic Tissue cells and method of use thereof |
| EP2158356A4 (en) * | 2007-05-04 | 2013-07-31 | Quebec Centre Rech Ind | SYSTEM AND METHOD FOR OPTIMIZING THE REFINING OF A GRANULAR LIGNOCELLULOSIC MATERIAL |
| US20090061473A1 (en) * | 2007-08-29 | 2009-03-05 | Rajiv Krishna Saxena | Measurement of Carbonaceous Particles in Biological Samples |
| CA2700617C (en) * | 2007-09-28 | 2018-11-06 | Celgene Cellular Therapeutics | Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells |
| US8034329B2 (en) * | 2007-10-05 | 2011-10-11 | Advanced Technologies And Regenerative Medicine, Llc | Repair and regeneration of renal tissue using human umbilical cord tissue-derived cells |
| DK2217329T3 (en) * | 2007-11-07 | 2018-04-23 | Anthrogenesis Corp | APPLICATION OF NAVLINE BLOOD IN THE TREATMENT OF COMPLIANCES IN CONNECTION WITH PREGNANCY BIRTH |
| US8236538B2 (en) | 2007-12-20 | 2012-08-07 | Advanced Technologies And Regenerative Medicine, Llc | Methods for sterilizing materials containing biologically active agents |
| US11051733B2 (en) * | 2008-01-18 | 2021-07-06 | Wake Forest University Health Sciences | Isolating and purifying cells for therapy |
| SG10201500237TA (en) | 2008-01-30 | 2015-04-29 | Histogen Inc | Extracellular matrix compositions |
| DK2330889T3 (en) * | 2008-08-20 | 2017-01-30 | Anthrogenesis Corp | Improved cell composition and process for making the same |
| JP5950577B2 (ja) * | 2008-08-20 | 2016-07-13 | アンスロジェネシス コーポレーション | 単離胎盤細胞を使用した脳卒中の治療 |
| HRP20130812T1 (en) * | 2008-08-22 | 2013-09-30 | Anthrogenesis Corporation | Methods and compositions for treatment of bone defects with placental cell populations |
| MX2011005230A (es) * | 2008-11-21 | 2011-06-16 | Anthrogenesis Corp | Tratamiento de enfermedades, desordenes o condiciones del pulmon usando celulas de placenta. |
| BRPI0923068B1 (pt) | 2008-12-19 | 2021-05-25 | Advanced Technologies And Regenerative Medicine, Llc | Uso de células derivadas de tecido de cordão umbilical para tratar um paciente tendo uma doença, distúrbio ou injúria pulmonar |
| WO2010071863A1 (en) * | 2008-12-19 | 2010-06-24 | Ethicon, Incorporated | Regeneration and repair of neural tissue following injury |
| PT2379087E (pt) * | 2008-12-19 | 2014-12-02 | Depuy Synthes Products Llc | Células derivadas do tecido do cordão umbilical para o tratamento da dor neuropática e espasticidade |
| US10179900B2 (en) * | 2008-12-19 | 2019-01-15 | DePuy Synthes Products, Inc. | Conditioned media and methods of making a conditioned media |
| CN102498204B (zh) * | 2009-03-26 | 2015-02-04 | 德普伊新特斯产品有限责任公司 | 人脐带组织细胞作为用于阿尔茨海默病的疗法 |
| WO2010118352A1 (en) * | 2009-04-09 | 2010-10-14 | Arizona Board Of Reagents On Behalf Of The University Of Arizona | Cellular seeding and co-culture of a three dimensional fibroblast construct |
| ES2646750T3 (es) * | 2010-01-26 | 2017-12-15 | Anthrogenesis Corporation | Tratamiento de cánceres relacionados con hueso utilizando células madre placentarias |
| IL250234B2 (en) | 2010-04-07 | 2023-10-01 | Anthrogenesis Llc | Angiogenesis using placental stem cells |
| MX2012011543A (es) | 2010-04-08 | 2013-05-06 | Anthrogenesis Corp | Tratamiento de sarcoidosis empleando celulas madre placentarias. |
| KR20180099915A (ko) | 2010-07-13 | 2018-09-05 | 안트로제네시스 코포레이션 | 천연 킬러 세포의 생성 방법 |
| US8895291B2 (en) | 2010-10-08 | 2014-11-25 | Terumo Bct, Inc. | Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions |
| AU2011352036A1 (en) | 2010-12-31 | 2013-07-18 | Anthrogenesis Corporation | Enhancement of placental stem cell potency using modulatory RNA molecules |
| CN104220081A (zh) | 2011-06-01 | 2014-12-17 | 人类起源公司 | 利用胎盘干细胞治疗疼痛 |
| US9844514B2 (en) * | 2011-09-30 | 2017-12-19 | University Of Louisville Research Foundation, Inc. | Methods for treating an established myocardial infarction |
| RU2466688C1 (ru) * | 2011-10-12 | 2012-11-20 | Ильгиз Фанусович Хатмуллин | Способ лечения послеоперационных анастомозов на органах брюшной полости, осложненных рубцовым стенозированием |
| JP6301263B2 (ja) | 2011-12-23 | 2018-03-28 | デピュイ・シンセス・プロダクツ・インコーポレイテッド | ヒト臍帯組織由来細胞の検出 |
| JP2015529523A (ja) * | 2012-09-04 | 2015-10-08 | アントフロゲネシス コーポレーション | 組織製造方法 |
| AU2014215458A1 (en) | 2013-02-05 | 2015-08-13 | Anthrogenesis Corporation | Natural killer cells from placenta |
| JP6612227B2 (ja) | 2013-11-16 | 2019-11-27 | テルモ ビーシーティー、インコーポレーテッド | バイオリアクターにおける細胞増殖 |
| US11008547B2 (en) | 2014-03-25 | 2021-05-18 | Terumo Bct, Inc. | Passive replacement of media |
| WO2016049421A1 (en) | 2014-09-26 | 2016-03-31 | Terumo Bct, Inc. | Scheduled feed |
| WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
| WO2017205667A1 (en) | 2016-05-25 | 2017-11-30 | Terumo Bct, Inc. | Cell expansion |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| EP3504315A4 (en) | 2016-08-27 | 2020-04-15 | 3D Biotek, LLC | BIOREACTOR |
| US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
| WO2018184028A2 (en) | 2017-03-31 | 2018-10-04 | Terumo Bct, Inc. | Cell expansion |
| US12234441B2 (en) | 2017-03-31 | 2025-02-25 | Terumo Bct, Inc. | Cell expansion |
| JP2020524002A (ja) | 2017-06-16 | 2020-08-13 | エイブリィ セラピューティクス インコーポレイテッド | 三次元組織組成物及び使用方法 |
| CN107308504A (zh) * | 2017-06-17 | 2017-11-03 | 常州帝君金属构件厂 | 一种心肌仿生支架的制备方法 |
| US11718829B2 (en) | 2017-07-28 | 2023-08-08 | Breakthrough Tech Llc | Methods and compositions for manufacturing extracellular matrix |
| EP3709930A4 (en) * | 2017-11-17 | 2021-09-01 | Arizona Board of Regents on behalf of the University of Arizona | Scaffolds having material properties optimized for cardiac applications and uses thereof |
| CN112384200A (zh) | 2018-01-30 | 2021-02-19 | 希斯托金公司 | 低氧条件下培养的细胞衍生的胞外囊泡及其用途 |
| US12077780B2 (en) | 2020-02-14 | 2024-09-03 | Allergan Sales, Llc | Conditioned medium from cells cultured under hypoxic conditions and uses thereof |
| GB2619893A (en) | 2021-03-23 | 2023-12-20 | Terumo Bct Inc | Cell capture and expansion |
| US12209689B2 (en) | 2022-02-28 | 2025-01-28 | Terumo Kabushiki Kaisha | Multiple-tube pinch valve assembly |
| USD1099116S1 (en) | 2022-09-01 | 2025-10-21 | Terumo Bct, Inc. | Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device |
Family Cites Families (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT359652B (de) * | 1979-02-15 | 1980-11-25 | Immuno Ag | Verfahren zur herstellung eines gewebekleb- stoffes |
| AT366916B (de) * | 1980-04-02 | 1982-05-25 | Immuno Ag | Vorrichtung zur applikation eines gewebeklebstoffes auf basis von menschlichen oder tierischenproteinen |
| US4627879A (en) * | 1984-09-07 | 1986-12-09 | The Trustees Of Columbia University In The City Of New York | Fibrin adhesive prepared as a concentrate from single donor fresh frozen plasma |
| US5266480A (en) * | 1986-04-18 | 1993-11-30 | Advanced Tissue Sciences, Inc. | Three-dimensional skin culture system |
| US5902741A (en) * | 1986-04-18 | 1999-05-11 | Advanced Tissue Sciences, Inc. | Three-dimensional cartilage cultures |
| US4963489A (en) * | 1987-04-14 | 1990-10-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US5032508A (en) * | 1988-09-08 | 1991-07-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US5863531A (en) * | 1986-04-18 | 1999-01-26 | Advanced Tissue Sciences, Inc. | In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework |
| US4721096A (en) * | 1986-04-18 | 1988-01-26 | Marrow-Tech Incorporated | Process for replicating bone marrow in vitro and using the same |
| US5290552A (en) * | 1988-05-02 | 1994-03-01 | Matrix Pharmaceutical, Inc./Project Hear | Surgical adhesive material |
| US4874368A (en) * | 1988-07-25 | 1989-10-17 | Micromedics, Inc. | Fibrin glue delivery system |
| JPH0762078B2 (ja) | 1989-03-03 | 1995-07-05 | 松下電器産業株式会社 | 有機薄膜の形成方法 |
| JP2678945B2 (ja) * | 1989-04-17 | 1997-11-19 | 有限会社ナイセム | 人工血管とその製造方法及び人工血管用基質 |
| US5318524A (en) * | 1990-01-03 | 1994-06-07 | Cryolife, Inc. | Fibrin sealant delivery kit |
| US5368563A (en) * | 1991-12-18 | 1994-11-29 | Micromedics, Inc. | Sprayer assembly for physiologic glue |
| US5478739A (en) * | 1992-10-23 | 1995-12-26 | Advanced Tissue Sciences, Inc. | Three-dimensional stromal cell and tissue culture system |
| GB9306449D0 (en) * | 1993-03-29 | 1993-05-19 | Nat Heart Research Fund | Tissue equivalents |
| US5709854A (en) * | 1993-04-30 | 1998-01-20 | Massachusetts Institute Of Technology | Tissue formation by injecting a cell-polymeric solution that gels in vivo |
| WO1995025547A1 (en) * | 1994-03-24 | 1995-09-28 | University Of Washington | Devices and methods for implanting transduced cells |
| EP0781116A4 (en) * | 1994-09-12 | 1999-08-25 | Advanced Tissue Sciences Inc | THREE-DIMENSIONAL CULTURES OF HUMAN CELLS ON HEART VALVE FRAME AND THEIR USE |
| US5605541A (en) * | 1994-12-07 | 1997-02-25 | E. R. Squibb And Sons, Inc. | Fibrin sealant applicatoor |
| US5643192A (en) * | 1995-04-06 | 1997-07-01 | Hamilton Civic Hospitals Research Development, Inc. | Autologous fibrin glue and methods for its preparation and use |
| US6284284B1 (en) * | 1995-06-06 | 2001-09-04 | Advanced Tissue Sciences, Inc. | Compositions and methods for production and use of an injectable naturally secreted extracellular matrix |
| US5830708A (en) * | 1995-06-06 | 1998-11-03 | Advanced Tissue Sciences, Inc. | Methods for production of a naturally secreted extracellular matrix |
| US5843766A (en) * | 1995-06-07 | 1998-12-01 | Advanced Tissue Sciences, Inc. | Apparatus for the growth and packaging of three dimensional tissue cultures |
| US5763267A (en) * | 1996-04-16 | 1998-06-09 | Advanced Tissue Sciences | Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures |
| US5759171A (en) * | 1996-09-27 | 1998-06-02 | Thermogenesis Corp. | Sprayer for fibrin glue |
| US6099832A (en) * | 1997-05-28 | 2000-08-08 | Genzyme Corporation | Transplants for myocardial scars |
| EP0989867B1 (de) * | 1997-06-27 | 2002-04-24 | BADER, Augustinus | Bioartifizielles transplantat und verfahren zu seiner herstellung |
| US6886568B2 (en) * | 1998-04-08 | 2005-05-03 | The Johns Hopkins University | Method for fabricating cell-containing implants |
| US6303136B1 (en) | 1998-04-13 | 2001-10-16 | Neurotech S.A. | Cells or tissue attached to a non-degradable filamentous matrix encapsulated by a semi-permeable membrane |
| EP1144596A2 (en) * | 1998-12-11 | 2001-10-17 | Advance Tissue Sciences Inc. | Application of shear flow stress to smooth muscle cells for the production of implantable structures |
| US6662805B2 (en) * | 1999-03-24 | 2003-12-16 | The Johns Hopkins University | Method for composite cell-based implants |
| US20030007954A1 (en) | 1999-04-12 | 2003-01-09 | Gail K. Naughton | Methods for using a three-dimensional stromal tissue to promote angiogenesis |
| US6372494B1 (en) * | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| US6461631B1 (en) * | 1999-11-16 | 2002-10-08 | Atrix Laboratories, Inc. | Biodegradable polymer composition |
| US6730016B1 (en) * | 2000-06-12 | 2004-05-04 | Acorn Cardiovascular, Inc. | Cardiac disease treatment and device |
| US6719970B1 (en) * | 2000-07-10 | 2004-04-13 | Alkermes Controlled Therapeutics, Inc. | Method of generating cartilage |
| US20030211088A1 (en) * | 2001-03-12 | 2003-11-13 | Field Loren J. | Multipotent cell and cardiomyocyte cell populations, and routes to and uses of same |
| IL158622A0 (en) * | 2001-04-30 | 2004-05-12 | Yissum Res Dev Co | Composite scaffolds and methods using same for generating complex tissue grafts |
| US20030003089A1 (en) * | 2001-05-11 | 2003-01-02 | Akins Robert E. | Tissue engineered cardiac constructs |
| ATE408666T1 (de) | 2002-05-28 | 2008-10-15 | Toyo Boseki | Verfahren zur kultur, zum speichern und zur induzierung von differenzierung von zellen und gerät zur verwendung in diesem verfahren, und dazugehöriges gebrauchsverfahren. |
| KR100483863B1 (ko) | 2002-12-27 | 2005-04-15 | 메디칸(주) | 고체분말을 이용한 3차원 세포 배양방법 |
| KR101310578B1 (ko) | 2003-02-20 | 2013-09-23 | 사이토리 테라퓨틱스, 인크. | 심혈관 증상의 치료에 지방조직-유래 세포를 사용하는 방법 |
| WO2006026617A2 (en) * | 2004-08-30 | 2006-03-09 | Iken Tissue Therapeutics, Inc. | Compositions and methods of promoting hair growth |
| ATE500855T1 (de) * | 2004-11-22 | 2011-03-15 | Theregen Inc | Vewendung von dreidimensionalem kultiviertem gewebe zur behandlung von kongestivem herzversagen |
| CA2612188A1 (en) * | 2005-06-17 | 2007-01-04 | Theregen, Inc. | Methods for treating ischemic tissue |
-
1999
- 1999-10-01 US US09/411,585 patent/US20030007954A1/en not_active Abandoned
-
2000
- 2000-04-12 AU AU44564/00A patent/AU777853C/en not_active Expired
- 2000-04-12 JP JP2000610535A patent/JP4709393B2/ja not_active Expired - Lifetime
- 2000-04-12 WO PCT/US2000/009848 patent/WO2000061204A1/en not_active Ceased
- 2000-04-12 EP EP00925950A patent/EP1169069B1/en not_active Expired - Lifetime
- 2000-04-12 DE DE60033381T patent/DE60033381T2/de not_active Expired - Lifetime
- 2000-04-12 IL IL14589200A patent/IL145892A0/xx unknown
- 2000-04-12 AT AT00925950T patent/ATE353676T1/de not_active IP Right Cessation
- 2000-04-12 CA CA002367507A patent/CA2367507C/en not_active Expired - Lifetime
- 2000-04-12 ES ES00925950T patent/ES2282105T3/es not_active Expired - Lifetime
-
2001
- 2001-10-11 IL IL145892A patent/IL145892A/en not_active IP Right Cessation
-
2004
- 2004-05-21 US US10/851,938 patent/US20040219134A1/en not_active Abandoned
-
2009
- 2009-04-08 US US12/420,716 patent/US8128924B2/en not_active Expired - Fee Related
-
2010
- 2010-12-29 US US12/981,260 patent/US20110213470A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2367507A1 (en) | 2000-10-19 |
| US8128924B2 (en) | 2012-03-06 |
| IL145892A (en) | 2009-06-15 |
| US20110213470A1 (en) | 2011-09-01 |
| ATE353676T1 (de) | 2007-03-15 |
| AU4456400A (en) | 2000-11-14 |
| US20030007954A1 (en) | 2003-01-09 |
| JP4709393B2 (ja) | 2011-06-22 |
| CA2367507C (en) | 2009-12-29 |
| JP2002541221A (ja) | 2002-12-03 |
| AU777853B2 (en) | 2004-11-04 |
| WO2000061204A1 (en) | 2000-10-19 |
| EP1169069A1 (en) | 2002-01-09 |
| DE60033381D1 (de) | 2007-03-29 |
| ES2282105T3 (es) | 2007-10-16 |
| AU777853C (en) | 2005-08-11 |
| EP1169069B1 (en) | 2007-02-14 |
| US20040219134A1 (en) | 2004-11-04 |
| IL145892A0 (en) | 2002-07-25 |
| US20090269316A1 (en) | 2009-10-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4709393B2 (ja) | 三次元的間質組織 | |
| DE69615549T2 (de) | Kunsthaut, enthaltend als träger bioverträgliche materialen aus hyaluronsäurederivaten | |
| RU2470611C2 (ru) | Окклюдер для чрезкожной транслюминальной процедуры (варианты), способ чрезкожного транслюминального закрытия отверстия в сердце, способ активизации васкуляризации ткани млекопитающего in vivo и способ активизации заживления места анастомоза | |
| DE69927600T2 (de) | Biotechnisch konstruiertes gewebe und verfahren zu dessen produktion und benutzung | |
| KR101650957B1 (ko) | 세포외 기질 조성물 | |
| DE3751843T2 (de) | Herstellung von körperorganen durch kontroliertes zellwachstum auf künstlicher matrix | |
| DE69333466T2 (de) | Herstellung von transplantatgeweben aus extrazellulärer matrix | |
| DE69634454T2 (de) | Zusammensetzungen und Verfahren mit Bezug auf eine auf natürliche Weise sekretierte Matrix | |
| DE69626035T2 (de) | Ein biologisches material, das eine wirksame kultur von knochenmarkstammzellen, die teilweise oder vollständing in bindegewebszellen differenziert sind, enthält, und eine dreidimensionale, bioverträgliche und biologische abbaubare matrix, die aus einen hyaluronsäurederivat besteht | |
| DE60110110T2 (de) | Aus gewebe hergestellte gefässstrukturen | |
| JP2003518078A (ja) | 組織再生組成物 | |
| DE69803065T2 (de) | Zellkulturmatrix und biologisch abbaubare dreidimensionale matrix auf hyaluronsäurederivatbasis | |
| JP2004533857A (ja) | 心膜の抗癒着性(anti−adhesion)パッチ | |
| EP1077072A2 (de) | In vitro-Verfahren zum Herstellen einer homologen Herzklappen- oder Gefässprothese | |
| EP3275471A1 (en) | Three-dimensional structure for cardiac muscular tissue regeneration and manufacturing method therefor | |
| DE69913543T2 (de) | Dermales gerüst auf der grundlage eines neutralisierten chitosan-schwammes oder eines neutralsierten gemischten chitosan-kollagen-schwammes | |
| AU2010233169A1 (en) | Cellular seeding and co-culture of a three dimensional fibroblast construct | |
| WO2001091820A1 (de) | Verfahren zur herstellung eines bioartifiziellen transplantates | |
| DE4438015A1 (de) | Epithelzellenhaltiges Biomaterial und dessen Verwendung als Transplantat | |
| DE69820254T2 (de) | Dermales hüllgewebe in der wundheilung | |
| WO2004042038A1 (de) | Verfahren zur behandlung von erkranktem,degeneriertem oder geschädigtem gewebe unter verwendung von in vitro hergestelltem dreidimensionalem gewebe in kombination mit gewebezellen und/oder exogenen faktoren | |
| EP1184040A1 (de) | Hautmatrix zur Abdeckung und Regenerierung verletzter Hautpartien sowie Verfahren zu ihrer Herstellung | |
| DE112019002479B4 (de) | Verfahren zur herstellung einer kollagen-laminin-matrix zur heilung von hautgeschwüren, verbrennungen und wunden beim menschen | |
| WO2002078721A9 (de) | Zwei-komponenten zusammensetzungen zur in situ herstellung von fibroblasten und keratinozyten umfassenden zelltransplantaten | |
| EP1782849A2 (en) | Three-dimensional stromal tissue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |