DE3480411D1 - Molecular cloning and expression in industrial microorganism species - Google Patents

Molecular cloning and expression in industrial microorganism species

Info

Publication number
DE3480411D1
DE3480411D1 DE8484200990T DE3480411T DE3480411D1 DE 3480411 D1 DE3480411 D1 DE 3480411D1 DE 8484200990 T DE8484200990 T DE 8484200990T DE 3480411 T DE3480411 T DE 3480411T DE 3480411 D1 DE3480411 D1 DE 3480411D1
Authority
DE
Germany
Prior art keywords
expression
molecular cloning
microorganism species
industrial microorganism
industrial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8484200990T
Other languages
German (de)
English (en)
Inventor
Johan Pieter Marinus Sanders
Den Berg Johannes Abel Van
Peter Michael Andreoli
Yvonne Johanna Vos
Ee Jan Hendrik Van
Leo J S M Mulleners
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke DSM NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8190976&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3480411(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of DE3480411D1 publication Critical patent/DE3480411D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
DE8484200990T 1983-07-06 1984-07-06 Molecular cloning and expression in industrial microorganism species Expired DE3480411D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP83201016 1983-07-06

Publications (1)

Publication Number Publication Date
DE3480411D1 true DE3480411D1 (en) 1989-12-14

Family

ID=8190976

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8484200990T Expired DE3480411D1 (en) 1983-07-06 1984-07-06 Molecular cloning and expression in industrial microorganism species

Country Status (11)

Country Link
EP (1) EP0134048B2 (enExample)
JP (2) JP2669457B2 (enExample)
AU (1) AU570709B2 (enExample)
DE (1) DE3480411D1 (enExample)
DK (1) DK169996B1 (enExample)
ES (2) ES8608579A1 (enExample)
FI (1) FI85287C (enExample)
GR (1) GR82200B (enExample)
IE (1) IE58014B1 (enExample)
NZ (1) NZ208806A (enExample)
WO (1) WO1985000382A1 (enExample)

Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
DE3484346D1 (de) * 1983-12-28 1991-05-02 Agency Ind Science Techn Dns-basensequenz enthaltende regionen einbegriffen in der herstellung und sekretion eines proteins, rekombinante dns das ganze oder einen teil der dns-basensequenz enthaltend und verfahren zur herstellung von proteinen unter verwendung der rekombinanten dns.
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
IE58099B1 (en) * 1984-06-06 1993-06-30 Cpc International Inc Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
US5024943A (en) * 1985-11-07 1991-06-18 Gist-Brocades Regulatory region cloning and analysis plasmid for bacillus
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
JP3026567B2 (ja) * 1987-02-27 2000-03-27 ジェネンコー インターナショナル インコーポレイテッド 分子のクローニング及びタンパク分解酵素をコードする遺伝子の発現
EP0896062A3 (en) * 1987-02-27 1999-04-07 Genencor International, Inc. Transformation of alkalophilic bacillus strains
RU2091487C1 (ru) * 1987-02-27 1997-09-27 Гист-Брокейдс Н.В. Способ получения штамма bacillus, содержащего две копии последовательности днк, кодирующей фермент с гидролазной активностью
KR0165550B1 (ko) * 1989-06-29 1999-01-15 마가렛트 에이. 호른 온도, 산 및/또는 알칼리에 대한 안정성이 증가된 변이체 미생물 알파-아밀라아제
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
CN100558898C (zh) 2000-09-21 2009-11-11 巴斯福股份公司 篮霉菌木聚糖酶
KR100867059B1 (ko) 2000-12-07 2008-11-04 디에스엠 아이피 어셋츠 비.브이. 카르복시 말단 프롤린 잔기를 갖는 펩티드가 풍부한단백질 가수분해물
WO2002046381A2 (en) 2000-12-07 2002-06-13 Dsm N.V. Method for the prevention or reduction of haze in beverages
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
EP3023501A1 (en) 2002-03-29 2016-05-25 Danisco US Inc. Enhanced protein expression in bacillus
ES2525873T3 (es) 2002-06-07 2014-12-30 Dsm Ip Assets B.V. Método mejorado para la prevención o reducción del enturbiamiento en bebidas
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
WO2004101763A2 (en) 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme lip1
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
NZ568554A (en) 2005-11-28 2011-12-22 Dsm Ip Assets Bv Lactase enzyme preparations yielding a clean taste
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
EP2113027B1 (en) 2007-02-20 2014-06-18 DSM IP Assets B.V. Novel sialidase
US8779112B2 (en) 2007-03-12 2014-07-15 Danisco Us Inc. Modified protease propeptides
JP2010521188A (ja) 2007-03-22 2010-06-24 ディーエスエム アイピー アセッツ ビー．ブイ． 新規リシルオキシダーゼ
CN101679489B (zh) 2007-05-10 2014-05-28 丹尼斯科美国公司 增加细菌内多肽表达的修饰的分泌系统
GB0715751D0 (en) * 2007-08-13 2007-09-19 Tmo Renewables Ltd Thermophilic micro-organisms for ethanol production
RU2515112C2 (ru) 2007-12-21 2014-05-10 ДАНИСКО ЮЭс ИНК. Улучшенная продукция белка в bacillus
KR101683302B1 (ko) 2008-03-28 2016-12-06 다니스코 유에스 인크. 박테리아 세포 내 로커스 증폭 방법
WO2010123754A1 (en) 2009-04-24 2010-10-28 Danisco Us Inc. Proteases with modified pro regions
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
CN105368809A (zh) 2009-12-09 2016-03-02 丹尼斯科美国公司 包含蛋白酶变体的组合物和方法
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
JP6018044B2 (ja) 2010-04-15 2016-11-02 ダニスコ・ユーエス・インク プロテアーゼ変異体を含む組成物及び方法
PL3095861T3 (pl) 2010-05-06 2020-04-30 The Procter & Gamble Company Produkty konsumenckie z odmianami proteaz
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
EP4230735A1 (en) 2011-05-05 2023-08-23 Danisco US Inc. Compositions and methods comprising serine protease variants
MX357386B (es) 2011-05-05 2018-07-06 Procter & Gamble Composiciones y metodos que comprenden variantes de proteasa serina.
CN102296042B (zh) * 2011-08-26 2013-04-24 甘肃农业大学 地衣芽孢杆菌及用其制备凝乳酶冻干粉的方法
US20140187468A1 (en) 2011-08-31 2014-07-03 Danisco Us Inc. Compositions and Methods Comprising a Lipolytic Enzyme Variant
US20150017700A1 (en) 2011-12-22 2015-01-15 Danisco Us Inc. Compositions and methods comprising a lipolytic enzyme variant
CA2887898A1 (en) 2012-10-12 2014-04-17 Danisco Us Inc. Compositions and methods comprising a lipolytic enzyme variant
EP2914720B1 (en) 2012-11-05 2022-08-31 Danisco US Inc. Compositions and methods comprising thermolysin protease variants
WO2014100018A1 (en) 2012-12-19 2014-06-26 Danisco Us Inc. Novel mannanase, compositions and methods of use thereof
EP3882346B1 (en) 2013-05-29 2025-10-01 Danisco US Inc. Novel metalloproteases
EP3636662B1 (en) 2013-05-29 2022-07-13 Danisco US Inc. Novel metalloproteases
US20160160202A1 (en) 2013-05-29 2016-06-09 Danisco Us Inc. Novel metalloproteases
JP6367930B2 (ja) 2013-05-29 2018-08-01 ダニスコ・ユーエス・インク 新規メタロプロテアーゼ
MX384253B (es) 2013-07-19 2025-03-14 Danisco Us Inc Composiciones y metodos que comprenden una variante de enzima lipolitica
JP6678108B2 (ja) 2013-09-12 2020-04-08 ダニスコ・ユーエス・インク Ｌｇ１２−系統群プロテアーゼ変異体を含む組成物及び方法
DK3080263T3 (da) 2013-12-13 2019-10-07 Danisco Us Inc Serinproteaser af bacillus gibsonii-clade
ES2723948T3 (es) 2013-12-13 2019-09-04 Danisco Us Inc Serina proteasas procedentes de especies de Bacillus
WO2015102814A1 (en) 2013-12-31 2015-07-09 Danisco Us Inc. Enhanced protein expression
CN106170546A (zh) 2014-03-21 2016-11-30 丹尼斯科美国公司 芽孢杆菌属的丝氨酸蛋白酶
WO2016061438A1 (en) 2014-10-17 2016-04-21 Danisco Us Inc. Serine proteases of bacillus species
CN107148472A (zh) 2014-10-27 2017-09-08 丹尼斯科美国公司 芽孢杆菌属物种的丝氨酸蛋白酶
EP3550017B1 (en) 2014-10-27 2021-07-14 Danisco US Inc. Serine proteases
US20170335306A1 (en) 2014-10-27 2017-11-23 Danisco Us Inc. Serine proteases
EP3212783B1 (en) 2014-10-27 2024-06-26 Danisco US Inc. Serine proteases
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
EP3233893B1 (en) 2014-12-16 2021-01-20 Danisco US Inc. Enhanced protein expression
US20170369537A1 (en) 2014-12-19 2017-12-28 Danisco Us Inc. Enhanced protein expression
EP3611259A1 (en) 2015-03-12 2020-02-19 Danisco US Inc. Compositions and methods comprising lg12-clade protease variants
EP4219704A3 (en) 2015-05-13 2023-08-23 Danisco US Inc Aprl-clade protease variants and uses thereof
US10731144B2 (en) 2015-06-17 2020-08-04 Danisco Us Inc Proteases with modified propeptide regions
EP3310911B1 (en) 2015-06-17 2023-03-15 Danisco US Inc. Bacillus gibsonii-clade serine proteases
JP2019518440A (ja) 2016-05-03 2019-07-04 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
US20190136218A1 (en) 2016-05-05 2019-05-09 Danisco Us Inc Protease variants and uses thereof
US11661567B2 (en) 2016-05-31 2023-05-30 Danisco Us Inc. Protease variants and uses thereof
CA3027745A1 (en) 2016-06-17 2017-12-21 Danisco Us Inc. Protease variants and uses thereof
WO2018118917A1 (en) 2016-12-21 2018-06-28 Danisco Us Inc. Protease variants and uses thereof
CN110312794B (zh) 2016-12-21 2024-04-12 丹尼斯科美国公司 吉氏芽孢杆菌进化枝丝氨酸蛋白酶
WO2019108599A1 (en) 2017-11-29 2019-06-06 Danisco Us Inc Subtilisin variants having improved stability
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
EP3810767A1 (en) 2018-06-19 2021-04-28 Danisco US Inc. Subtilisin variants
US20240279627A1 (en) 2018-08-30 2024-08-22 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
US20230028935A1 (en) 2018-11-28 2023-01-26 Danisco Us Inc Subtilisin variants having improved stability
WO2020242858A1 (en) 2019-05-24 2020-12-03 Danisco Us Inc Subtilisin variants and methods of use
US20230049452A1 (en) 2020-01-13 2023-02-16 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
US20240294888A1 (en) 2021-06-30 2024-09-05 Danisco Us Inc. Variant enzymes and uses thereof
WO2023034486A2 (en) 2021-09-03 2023-03-09 Danisco Us Inc. Laundry compositions for cleaning
JP2025502641A (ja) 2021-12-16 2025-01-28 ザ プロクター アンド ギャンブル カンパニー プロテアーゼを含む自動食器洗浄組成物
WO2023114932A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448708A1 (en) 2021-12-16 2024-10-23 The Procter & Gamble Company Fabric and home care composition comprising a protease
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448751A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and methods of use
CN118974227A (zh) 2022-03-01 2024-11-15 丹尼斯科美国公司 用于清洁的酶和酶组合物
CN119855892A (zh) 2022-09-02 2025-04-18 丹尼斯科美国公司 洗涤剂组合物及其相关方法
CA3265718A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc VARIANTS OF SUBTILISINE AND ASSOCIATED PROCESSES
EP4615968A1 (en) 2022-11-09 2025-09-17 Danisco US Inc. Subtilisin variants and methods of use
EP4410941A1 (en) 2023-02-01 2024-08-07 The Procter & Gamble Company Detergent compositions containing enzymes
CN120677237A (zh) 2023-02-01 2025-09-19 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
CN120712348A (zh) 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
ZA81424B (en) * 1980-02-15 1982-02-24 Cpc International Inc Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
JPS56137896A (en) * 1980-03-10 1981-10-28 Cetus Corp Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1206108A (en) * 1981-03-06 1986-06-17 Shuichi Aiba Process for transforming microorganism by means of plasmid introduction
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
JPS5889194A (ja) * 1981-11-24 1983-05-27 Ajinomoto Co Inc 微生物によるｌ−トリプトフアンの製造法
JPS58107192A (ja) * 1981-12-18 1983-06-25 Ajinomoto Co Inc 発酵法によるｌ−ヒスチジンの製造法

Also Published As

Publication number Publication date
JPH06189783A (ja) 1994-07-12
ES545256A0 (es) 1986-08-01
ES8608579A1 (es) 1986-06-16
FI85287B (fi) 1991-12-13
GR82200B (enExample) 1984-12-13
AU3151084A (en) 1985-02-07
DK169996B1 (da) 1995-04-24
JPS60501738A (ja) 1985-10-17
IE841726L (en) 1985-01-06
IE58014B1 (en) 1993-06-16
ES534102A0 (es) 1986-06-16
EP0134048B1 (en) 1989-11-08
DK101385D0 (da) 1985-03-05
FI850845A0 (fi) 1985-03-01
JP2669457B2 (ja) 1997-10-27
NZ208806A (en) 1988-07-28
EP0134048A1 (en) 1985-03-13
FI850845L (fi) 1985-03-01
EP0134048B2 (en) 1996-08-14
FI85287C (fi) 1992-03-25
ES8609467A1 (es) 1986-08-01
WO1985000382A1 (en) 1985-01-31
DK101385A (da) 1985-03-05
JP2664860B2 (ja) 1997-10-22
AU570709B2 (en) 1988-03-24

Similar Documents

Publication Publication Date Title
DE3480411D1 (en) Molecular cloning and expression in industrial microorganism species
DE3476085D1 (en) Microbial pathogen detecting system and process
AU551820B2 (en) Copolyester and textile treating composition
GB2134334B (en) Wrap-around recoverable article
EG17596A (en) Disinfection method and composition therefor
GB8615628D0 (en) Arrangement in industrial trucks
GB2178714B (en) Improvements in or relating to multiple station workpiece processing lines
AU4876585A (en) Peptide anitbodies and their use in detecting oncogene products
GB2145055B (en) Improvements in or relating to an industrial robot
EP0133657A3 (en) Thermoplastic composition and process
FI844801A7 (fi) Parannus vankomysiinin puhdistukseen ja puhdistustuote.
YU104684A (en) Tool for manual inoculating in fruit-growing and wine-growing
DE2961090D1 (en) Processing of foodstuffs and composition for the protection of microorganisms used in foodstuff-processing
GB2145110B (en) Alcohol-free wine and its manufacture
IL73055A (en) Molecular electro-optical transistor and switch
GB2136938B (en) Improvements in furnaces
DE3475417D1 (en) Improvements in and/or relating to cribwalling
AU6042286A (en) Improvements in handling harmful substances - removing asbestos
GB9212261D0 (en) Enzyme cloning
EP0174981A4 (en) PROCESS FOR TREATING DEPRESSION IN VERTEBRES.
ZA844621B (en) Novel mutant microorganism and its use in removing organic sulfur compounds
IL79701A (en) Clear polyvinyl chloride articles and compositions
AU3152284A (en) Einrichtung zur vorreinigung von organisch belasteten abfallsubstraten in geloster und fester formbei gleichzeitiger gewinnung von methangas
GB8719832D0 (en) 2-methyl-5-isopropylhexa-2-5-dien-1-al in microorganisms
GB8428883D0 (en) Bearing arrangement in o-configuration

Legal Events

Date Code Title Description
8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings
8327 Change in the person/name/address of the patent owner

Owner name: DSM N.V., HEERLEN, NL

8310 Action for declaration of annulment
8313 Request for invalidation rejected/withdrawn