DE2825595A1 - Verfahren zur reinigung von nucleotidsequenzen und eine so erhaltene nucleotidsequenz enthaltende mikroorganismen - Google Patents
Verfahren zur reinigung von nucleotidsequenzen und eine so erhaltene nucleotidsequenz enthaltende mikroorganismenInfo
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- DE2825595A1 DE2825595A1 DE19782825595 DE2825595A DE2825595A1 DE 2825595 A1 DE2825595 A1 DE 2825595A1 DE 19782825595 DE19782825595 DE 19782825595 DE 2825595 A DE2825595 A DE 2825595A DE 2825595 A1 DE2825595 A1 DE 2825595A1
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- CTCBPRXHVPZNHB-VQFZJOCSSA-N [[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate;(2r,3r,4s,5r)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O.C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O CTCBPRXHVPZNHB-VQFZJOCSSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 208000017529 benign neoplasm of pituitary gland Diseases 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 208000016861 hereditary angioedema type 3 Diseases 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000007169 ligase reaction Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000019988 mead Nutrition 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000003169 placental effect Effects 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000002601 radiography Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Pregnancy & Childbirth (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Diabetes (AREA)
- Bioinformatics & Computational Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83621877A | 1977-09-23 | 1977-09-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2825595A1 true DE2825595A1 (de) | 1979-04-12 |
Family
ID=25271469
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19782825595 Withdrawn DE2825595A1 (de) | 1977-09-23 | 1978-06-10 | Verfahren zur reinigung von nucleotidsequenzen und eine so erhaltene nucleotidsequenz enthaltende mikroorganismen |
Country Status (25)
| Country | Link |
|---|---|
| JP (1) | JPS5448775A (ja) |
| AT (2) | AT369386B (ja) |
| AU (1) | AU519528B2 (ja) |
| BE (1) | BE868853A (ja) |
| CS (1) | CS235069B2 (ja) |
| DD (2) | DD153393A5 (ja) |
| DE (1) | DE2825595A1 (ja) |
| DK (1) | DK233378A (ja) |
| ES (1) | ES470340A1 (ja) |
| FI (1) | FI64187C (ja) |
| FR (1) | FR2404013A1 (ja) |
| GB (1) | GB1568047A (ja) |
| GR (1) | GR73558B (ja) |
| IE (1) | IE47332B1 (ja) |
| IL (1) | IL54791A (ja) |
| IT (1) | IT1098340B (ja) |
| LU (1) | LU79938A1 (ja) |
| NL (1) | NL7806358A (ja) |
| NZ (1) | NZ187342A (ja) |
| PL (1) | PL209748A1 (ja) |
| PT (1) | PT68127A (ja) |
| RO (2) | RO78407A (ja) |
| SE (3) | SE7806087L (ja) |
| YU (1) | YU214378A (ja) |
| ZA (1) | ZA782933B (ja) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0020147A1 (en) * | 1979-06-01 | 1980-12-10 | The Regents Of The University Of California | A DNA transfer vector for human pre-growth hormone, a microorganism transformed thereby, and a method of cloning therefor |
| DE3220333A1 (de) * | 1981-06-02 | 1983-01-27 | Wakunaga Yakuhin K.K., Osaka | 27-desamidosecretin und verfahren zu seiner herstellung durch rekombinierte dna-technologie |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422717B1 (fr) * | 1977-11-08 | 1985-12-06 | Genentech Inc | Procede permettant l'expression microbienne de polypeptides et moyens appropries |
| US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| US4530901A (en) * | 1980-01-08 | 1985-07-23 | Biogen N.V. | Recombinant DNA molecules and their use in producing human interferon-like polypeptides |
| US6835557B1 (en) | 1980-01-08 | 2004-12-28 | Biogen, Inc. | DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides |
| IL59690A (en) | 1980-03-24 | 1983-11-30 | Yeda Res & Dev | Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it |
| ES500966A0 (es) | 1980-04-03 | 1982-12-16 | Biogen Nv | Un metodo de producir un polipeptido que muestra una actividad immunologica obiologica de interferon de fibroblasto hu- mano. |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| US5670371A (en) * | 1983-07-15 | 1997-09-23 | Bio-Technology General Corp. | Bacterial expression of superoxide dismutase |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ187300A (en) * | 1977-05-27 | 1982-08-17 | Univ California | Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism |
| SK278170B6 (en) * | 1977-11-08 | 1996-03-06 | Keiichi Itakura | Recombinant plasmid, method of its preparation and bacteria transformed by them |
-
1978
- 1978-05-22 ZA ZA00782933A patent/ZA782933B/xx unknown
- 1978-05-23 GR GR56310A patent/GR73558B/el unknown
- 1978-05-23 NZ NZ187342A patent/NZ187342A/xx unknown
- 1978-05-25 AU AU36489/78A patent/AU519528B2/en not_active Expired
- 1978-05-25 GB GB22418/78A patent/GB1568047A/en not_active Expired
- 1978-05-26 DK DK233378A patent/DK233378A/da not_active Application Discontinuation
- 1978-05-26 IL IL54791A patent/IL54791A/xx unknown
- 1978-05-26 FI FI781676A patent/FI64187C/fi not_active IP Right Cessation
- 1978-05-26 IE IE1054/78A patent/IE47332B1/en unknown
- 1978-05-26 SE SE7806087A patent/SE7806087L/xx unknown
- 1978-05-27 RO RO7894192A patent/RO78407A/ro unknown
- 1978-05-27 RO RO78105866A patent/RO80006A/ro unknown
- 1978-05-30 ES ES470340A patent/ES470340A1/es not_active Expired
- 1978-06-01 PT PT68127A patent/PT68127A/pt unknown
- 1978-06-05 JP JP6756678A patent/JPS5448775A/ja active Pending
- 1978-06-07 CS CS783704A patent/CS235069B2/cs unknown
- 1978-06-10 DE DE19782825595 patent/DE2825595A1/de not_active Withdrawn
- 1978-06-12 NL NL7806358A patent/NL7806358A/xx not_active Application Discontinuation
- 1978-06-16 IT IT24641/78A patent/IT1098340B/it active
- 1978-06-22 AT AT0456678A patent/AT369386B/de not_active IP Right Cessation
- 1978-07-03 DD DD78224374A patent/DD153393A5/de unknown
- 1978-07-03 DD DD78206482A patent/DD145917A5/de unknown
- 1978-07-04 FR FR7819901A patent/FR2404013A1/fr active Granted
- 1978-07-07 BE BE189169A patent/BE868853A/xx not_active IP Right Cessation
- 1978-07-07 LU LU79938A patent/LU79938A1/xx unknown
- 1978-09-11 YU YU02143/78A patent/YU214378A/xx unknown
- 1978-09-21 PL PL20974878A patent/PL209748A1/xx unknown
-
1981
- 1981-06-03 AT AT0248881A patent/AT375673B/de not_active IP Right Cessation
-
1983
- 1983-09-23 SE SE8305162A patent/SE8305162L/sv not_active Application Discontinuation
- 1983-09-23 SE SE8305163A patent/SE8305163L/sv not_active Application Discontinuation
Non-Patent Citations (1)
| Title |
|---|
| NICHTS-ERMITTELT * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0020147A1 (en) * | 1979-06-01 | 1980-12-10 | The Regents Of The University Of California | A DNA transfer vector for human pre-growth hormone, a microorganism transformed thereby, and a method of cloning therefor |
| DE3220333A1 (de) * | 1981-06-02 | 1983-01-27 | Wakunaga Yakuhin K.K., Osaka | 27-desamidosecretin und verfahren zu seiner herstellung durch rekombinierte dna-technologie |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8130 | Withdrawal |