ES470340A1 - Un metodo para obtener una cepa de microorganismos modifica-dos para contener un fragmento de una secuencia especifica de desoxiribonucleotidos - Google Patents
Un metodo para obtener una cepa de microorganismos modifica-dos para contener un fragmento de una secuencia especifica de desoxiribonucleotidosInfo
- Publication number
- ES470340A1 ES470340A1 ES470340A ES470340A ES470340A1 ES 470340 A1 ES470340 A1 ES 470340A1 ES 470340 A ES470340 A ES 470340A ES 470340 A ES470340 A ES 470340A ES 470340 A1 ES470340 A1 ES 470340A1
- Authority
- ES
- Spain
- Prior art keywords
- fragments
- dna
- sub
- length
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000894006 Bacteria Species 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 239000012634 fragment Substances 0.000 abstract 7
- 239000005547 deoxyribonucleotide Substances 0.000 abstract 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 244000005700 microbiome Species 0.000 abstract 2
- 108091008146 restriction endonucleases Proteins 0.000 abstract 2
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormones [GH] (Somatotropin)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Pregnancy & Childbirth (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Diabetes (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Un método para obtener una cepa de microorganismos modificados para contener un fragmento de una secuencia específica de desoxiribonucleótidos, cuyo método comprende: (I) purificar un fragmento de una secuencia de desoxiribonucleótidos mediante las siguientes etapas: (a) proveer una población de transcritos de ADNc de los polirribonucleótidos. (b) Someter los transcritos de ADNc a la acción de una preparación de endonucleasa de restricción capaz de catalizar la hidrólisis de los transcritos de ADNc en cada uno de los puntos de restricción. (c) fraccionar los fragmentos producidos por la acción de la endonucleasa de restricción de acuerdo con su longitud. (d) tratar previamente el ADN para eliminar cualquier grupo terminal 5''-fosfato. (e) incubar el ADN con una endonucelasa de restricción capaz de actuar sobre la secuencia específica deseada de nucleótidos para producir dos sub-fragmentos lineales del mismo. (f) fraccionar los sub-fragmentos de acuerdo con su longitud. (g) aislar los dos sub-fragmentos, (h) reunir los sub-fragmentos covalentemente, en una reacción catalizada por ADN ligasa, (i) fraccionar las moléculas de ADN reunidas de acuerdo con su longitud, (II) recombinar el producto procedente de la secuencia anterior con un vector de transferencia de ADN y transferirlo a un microorganismo.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83621877A | 1977-09-23 | 1977-09-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES470340A1 true ES470340A1 (es) | 1979-09-16 |
Family
ID=25271469
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES470340A Expired ES470340A1 (es) | 1977-09-23 | 1978-05-30 | Un metodo para obtener una cepa de microorganismos modifica-dos para contener un fragmento de una secuencia especifica de desoxiribonucleotidos |
Country Status (25)
| Country | Link |
|---|---|
| JP (1) | JPS5448775A (es) |
| AT (2) | AT369386B (es) |
| AU (1) | AU519528B2 (es) |
| BE (1) | BE868853A (es) |
| CS (1) | CS235069B2 (es) |
| DD (2) | DD145917A5 (es) |
| DE (1) | DE2825595A1 (es) |
| DK (1) | DK233378A (es) |
| ES (1) | ES470340A1 (es) |
| FI (1) | FI64187C (es) |
| FR (1) | FR2404013A1 (es) |
| GB (1) | GB1568047A (es) |
| GR (1) | GR73558B (es) |
| IE (1) | IE47332B1 (es) |
| IL (1) | IL54791A (es) |
| IT (1) | IT1098340B (es) |
| LU (1) | LU79938A1 (es) |
| NL (1) | NL7806358A (es) |
| NZ (1) | NZ187342A (es) |
| PL (1) | PL209748A1 (es) |
| PT (1) | PT68127A (es) |
| RO (2) | RO78407A (es) |
| SE (3) | SE7806087L (es) |
| YU (1) | YU214378A (es) |
| ZA (1) | ZA782933B (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422717B1 (fr) * | 1977-11-08 | 1985-12-06 | Genentech Inc | Procede permettant l'expression microbienne de polypeptides et moyens appropries |
| US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
| GR68404B (es) * | 1979-06-01 | 1981-12-29 | Univ California | |
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| US4530901A (en) * | 1980-01-08 | 1985-07-23 | Biogen N.V. | Recombinant DNA molecules and their use in producing human interferon-like polypeptides |
| US6835557B1 (en) | 1980-01-08 | 2004-12-28 | Biogen, Inc. | DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides |
| IL59690A (en) | 1980-03-24 | 1983-11-30 | Yeda Res & Dev | Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it |
| EP0041313B1 (en) | 1980-04-03 | 1990-09-12 | Biogen, Inc. | Dna sequences, recombinant dna molecules and processes for producing human fibroblast interferon |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| CH659826A5 (fr) * | 1981-06-02 | 1987-02-27 | Wakunaga Yakuhin Kk | Procede d'obtention d'un microorganisme transforme par un plasmide comprenant un gene de structure de la 27-desamidosecretine. |
| US5670371A (en) * | 1983-07-15 | 1997-09-23 | Bio-Technology General Corp. | Bacterial expression of superoxide dismutase |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ187300A (en) * | 1977-05-27 | 1982-08-17 | Univ California | Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism |
| NL7811040A (nl) * | 1977-11-08 | 1979-05-10 | Genentech Inc | Synthetische dna en werkwijze ter bereiding daarvan. |
-
1978
- 1978-05-22 ZA ZA00782933A patent/ZA782933B/xx unknown
- 1978-05-23 NZ NZ187342A patent/NZ187342A/xx unknown
- 1978-05-23 GR GR56310A patent/GR73558B/el unknown
- 1978-05-25 GB GB22418/78A patent/GB1568047A/en not_active Expired
- 1978-05-25 AU AU36489/78A patent/AU519528B2/en not_active Expired
- 1978-05-26 IL IL54791A patent/IL54791A/xx unknown
- 1978-05-26 DK DK233378A patent/DK233378A/da not_active Application Discontinuation
- 1978-05-26 IE IE1054/78A patent/IE47332B1/en unknown
- 1978-05-26 FI FI781676A patent/FI64187C/fi not_active IP Right Cessation
- 1978-05-26 SE SE7806087A patent/SE7806087L/xx unknown
- 1978-05-27 RO RO7894192A patent/RO78407A/ro unknown
- 1978-05-27 RO RO78105866A patent/RO80006A/ro unknown
- 1978-05-30 ES ES470340A patent/ES470340A1/es not_active Expired
- 1978-06-01 PT PT68127A patent/PT68127A/pt unknown
- 1978-06-05 JP JP6756678A patent/JPS5448775A/ja active Pending
- 1978-06-07 CS CS783704A patent/CS235069B2/cs unknown
- 1978-06-10 DE DE19782825595 patent/DE2825595A1/de not_active Withdrawn
- 1978-06-12 NL NL7806358A patent/NL7806358A/xx not_active Application Discontinuation
- 1978-06-16 IT IT24641/78A patent/IT1098340B/it active
- 1978-06-22 AT AT0456678A patent/AT369386B/de not_active IP Right Cessation
- 1978-07-03 DD DD78206482A patent/DD145917A5/de unknown
- 1978-07-03 DD DD78224374A patent/DD153393A5/de unknown
- 1978-07-04 FR FR7819901A patent/FR2404013A1/fr active Granted
- 1978-07-07 LU LU79938A patent/LU79938A1/xx unknown
- 1978-07-07 BE BE189169A patent/BE868853A/xx not_active IP Right Cessation
- 1978-09-11 YU YU02143/78A patent/YU214378A/xx unknown
- 1978-09-21 PL PL20974878A patent/PL209748A1/xx unknown
-
1981
- 1981-06-03 AT AT0248881A patent/AT375673B/de not_active IP Right Cessation
-
1983
- 1983-09-23 SE SE8305163A patent/SE8305163D0/xx not_active Application Discontinuation
- 1983-09-23 SE SE8305162A patent/SE8305162L/sv not_active Application Discontinuation
Also Published As
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD1A | Patent lapsed |
Effective date: 19980202 |