FR2404013A1 - Procede de purification de sequences de nucleotides, en vue de leur replication dans les bacteries - Google Patents
Procede de purification de sequences de nucleotides, en vue de leur replication dans les bacteriesInfo
- Publication number
- FR2404013A1 FR2404013A1 FR7819901A FR7819901A FR2404013A1 FR 2404013 A1 FR2404013 A1 FR 2404013A1 FR 7819901 A FR7819901 A FR 7819901A FR 7819901 A FR7819901 A FR 7819901A FR 2404013 A1 FR2404013 A1 FR 2404013A1
- Authority
- FR
- France
- Prior art keywords
- view
- length
- replication
- bacteria
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormones [GH] (Somatotropin)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Abstract
L'invention concerne un procédé de purification d'un fragment d'une séquence de nucléotides spécifique, à partir d'une population de polyribonucléotides de longueur et de séquence hétérogènes, lequel procédé consiste à former des cADN transcrits dont au moins une partie comporte au minimum deux sites de restriction, à soumettre ces cADN à l'action d'une endonucléase de restriction afin de produire un fragment de la séquence spécifique, de longueur homogène, à fractionner les fragments selon leur longueur, en vue de la recombinaison avec un vecteur de transfert d'ADN et du transfert ultérieur à un microorganisme.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US83621877A | 1977-09-23 | 1977-09-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2404013A1 true FR2404013A1 (fr) | 1979-04-20 |
FR2404013B1 FR2404013B1 (fr) | 1983-07-22 |
Family
ID=25271469
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7819901A Granted FR2404013A1 (fr) | 1977-09-23 | 1978-07-04 | Procede de purification de sequences de nucleotides, en vue de leur replication dans les bacteries |
Country Status (25)
Country | Link |
---|---|
JP (1) | JPS5448775A (fr) |
AT (2) | AT369386B (fr) |
AU (1) | AU519528B2 (fr) |
BE (1) | BE868853A (fr) |
CS (1) | CS235069B2 (fr) |
DD (2) | DD145917A5 (fr) |
DE (1) | DE2825595A1 (fr) |
DK (1) | DK233378A (fr) |
ES (1) | ES470340A1 (fr) |
FI (1) | FI64187C (fr) |
FR (1) | FR2404013A1 (fr) |
GB (1) | GB1568047A (fr) |
GR (1) | GR73558B (fr) |
IE (1) | IE47332B1 (fr) |
IL (1) | IL54791A (fr) |
IT (1) | IT1098340B (fr) |
LU (1) | LU79938A1 (fr) |
NL (1) | NL7806358A (fr) |
NZ (1) | NZ187342A (fr) |
PL (1) | PL209748A1 (fr) |
PT (1) | PT68127A (fr) |
RO (2) | RO78407A (fr) |
SE (3) | SE7806087L (fr) |
YU (1) | YU214378A (fr) |
ZA (1) | ZA782933B (fr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2422717B1 (fr) * | 1977-11-08 | 1985-12-06 | Genentech Inc | Procede permettant l'expression microbienne de polypeptides et moyens appropries |
US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
GR68404B (fr) * | 1979-06-01 | 1981-12-29 | Univ California | |
US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
US4530901A (en) * | 1980-01-08 | 1985-07-23 | Biogen N.V. | Recombinant DNA molecules and their use in producing human interferon-like polypeptides |
US6835557B1 (en) | 1980-01-08 | 2004-12-28 | Biogen, Inc. | DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides |
IL59690A (en) | 1980-03-24 | 1983-11-30 | Yeda Res & Dev | Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it |
EP0041313B1 (fr) | 1980-04-03 | 1990-09-12 | Biogen, Inc. | Séquences d'ADN, molécules d'ADN recombinant et procédé pour la production de l'interféron de fibroblaste humain |
US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
CH659826A5 (fr) * | 1981-06-02 | 1987-02-27 | Wakunaga Yakuhin Kk | Procede d'obtention d'un microorganisme transforme par un plasmide comprenant un gene de structure de la 27-desamidosecretine. |
US5670371A (en) * | 1983-07-15 | 1997-09-23 | Bio-Technology General Corp. | Bacterial expression of superoxide dismutase |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2392033A1 (fr) * | 1977-05-27 | 1978-12-22 | Univ California | Vecteur de transfert d'adn recombinant et microorganisme contenant un gene provenant d'un organisme superieur |
EP0001931A2 (fr) * | 1977-11-08 | 1979-05-16 | Genentech, Inc. | ADN synthétique, procédé de préparation et vecteur microbien clônant recombinant contenant cet ADN |
-
1978
- 1978-05-22 ZA ZA00782933A patent/ZA782933B/xx unknown
- 1978-05-23 NZ NZ187342A patent/NZ187342A/xx unknown
- 1978-05-23 GR GR56310A patent/GR73558B/el unknown
- 1978-05-25 GB GB22418/78A patent/GB1568047A/en not_active Expired
- 1978-05-25 AU AU36489/78A patent/AU519528B2/en not_active Expired
- 1978-05-26 IL IL54791A patent/IL54791A/xx unknown
- 1978-05-26 DK DK233378A patent/DK233378A/da not_active Application Discontinuation
- 1978-05-26 IE IE1054/78A patent/IE47332B1/en unknown
- 1978-05-26 FI FI781676A patent/FI64187C/fi not_active IP Right Cessation
- 1978-05-26 SE SE7806087A patent/SE7806087L/xx unknown
- 1978-05-27 RO RO7894192A patent/RO78407A/fr unknown
- 1978-05-27 RO RO78105866A patent/RO80006A/fr unknown
- 1978-05-30 ES ES470340A patent/ES470340A1/es not_active Expired
- 1978-06-01 PT PT68127A patent/PT68127A/pt unknown
- 1978-06-05 JP JP6756678A patent/JPS5448775A/ja active Pending
- 1978-06-07 CS CS783704A patent/CS235069B2/cs unknown
- 1978-06-10 DE DE19782825595 patent/DE2825595A1/de not_active Withdrawn
- 1978-06-12 NL NL7806358A patent/NL7806358A/xx not_active Application Discontinuation
- 1978-06-16 IT IT24641/78A patent/IT1098340B/it active
- 1978-06-22 AT AT0456678A patent/AT369386B/de not_active IP Right Cessation
- 1978-07-03 DD DD78206482A patent/DD145917A5/de unknown
- 1978-07-03 DD DD78224374A patent/DD153393A5/de unknown
- 1978-07-04 FR FR7819901A patent/FR2404013A1/fr active Granted
- 1978-07-07 LU LU79938A patent/LU79938A1/xx unknown
- 1978-07-07 BE BE189169A patent/BE868853A/fr not_active IP Right Cessation
- 1978-09-11 YU YU02143/78A patent/YU214378A/xx unknown
- 1978-09-21 PL PL20974878A patent/PL209748A1/xx unknown
-
1981
- 1981-06-03 AT AT0248881A patent/AT375673B/de not_active IP Right Cessation
-
1983
- 1983-09-23 SE SE8305163A patent/SE8305163D0/xx not_active Application Discontinuation
- 1983-09-23 SE SE8305162A patent/SE8305162L/sv not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2392033A1 (fr) * | 1977-05-27 | 1978-12-22 | Univ California | Vecteur de transfert d'adn recombinant et microorganisme contenant un gene provenant d'un organisme superieur |
EP0001931A2 (fr) * | 1977-11-08 | 1979-05-16 | Genentech, Inc. | ADN synthétique, procédé de préparation et vecteur microbien clônant recombinant contenant cet ADN |
Non-Patent Citations (3)
Title |
---|
CA1978 * |
EXBK/76 * |
EXBK/77 * |
Also Published As
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |