SE8305163L - Dna-overforingsvektor med tillvexthormonkodoner, forfarande for dess framstellning samt en nemnda vektor innehallande mikroorganism jemte dess framstellning - Google Patents
Dna-overforingsvektor med tillvexthormonkodoner, forfarande for dess framstellning samt en nemnda vektor innehallande mikroorganism jemte dess framstellningInfo
- Publication number
- SE8305163L SE8305163L SE8305163A SE8305163A SE8305163L SE 8305163 L SE8305163 L SE 8305163L SE 8305163 A SE8305163 A SE 8305163A SE 8305163 A SE8305163 A SE 8305163A SE 8305163 L SE8305163 L SE 8305163L
- Authority
- SE
- Sweden
- Prior art keywords
- cdna
- fragments
- population
- fragment
- sub
- Prior art date
Links
- 230000012010 growth Effects 0.000 title claims 4
- 239000013598 vector Substances 0.000 title claims 4
- 238000004519 manufacturing process Methods 0.000 title claims 3
- 238000000034 method Methods 0.000 title claims 2
- 230000005540 biological transmission Effects 0.000 title 1
- 244000005700 microbiome Species 0.000 title 1
- 239000012634 fragment Substances 0.000 claims 14
- 239000002299 complementary DNA Substances 0.000 claims 13
- 102000002265 Human Growth Hormone Human genes 0.000 claims 3
- 108010000521 Human Growth Hormone Proteins 0.000 claims 3
- 239000000854 Human Growth Hormone Substances 0.000 claims 3
- 238000011534 incubation Methods 0.000 claims 3
- 239000011541 reaction mixture Substances 0.000 claims 3
- 102000012410 DNA Ligases Human genes 0.000 claims 2
- 108010061982 DNA Ligases Proteins 0.000 claims 2
- 102000003960 Ligases Human genes 0.000 claims 2
- 108090000364 Ligases Proteins 0.000 claims 2
- 238000001502 gel electrophoresis Methods 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 1
- 235000005273 Canna coccinea Nutrition 0.000 claims 1
- 240000008555 Canna flaccida Species 0.000 claims 1
- 108020004635 Complementary DNA Proteins 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 102100031780 Endonuclease Human genes 0.000 claims 1
- 206010062767 Hypophysitis Diseases 0.000 claims 1
- 241000920340 Pion Species 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 238000006555 catalytic reaction Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 230000001817 pituitary effect Effects 0.000 claims 1
- 210000003635 pituitary gland Anatomy 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Pregnancy & Childbirth (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Diabetes (AREA)
- Bioinformatics & Computational Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (1)
1. -d) ails ev. 5'-fosfat5ndgrupper pi cDNA-fragmenten hydrolyseras, ten. genom att fragmenten inkuberas med alkaliskt fosfatas vid pH 7,5 och 60°C under 10 minuter, el namnda cDNA-fragment hydrolyseras enzymatiskt specifikt vid namnda restriktionsst011e genom :lnkubation av en reaktionsblandning, som innehaller namnda fragment och ett fir detta restriktionsstalle specifikt restriktionsendonukleas, t.ex. inkubation av namnda fragment med PvuIT vid pH 7,6 och 37°C under 2 timmar, varvid delfragment bildas, son utgOres av delar av namnda fragment, 6. delfragmenten fraktioneras alltefter sin langd, t.ex. medelst gelelektrofores, 7. man uppsamlar de fraktoner, son innehiller de bade fir humant tillvaxthormon kodande delfragmenten av namnda cDNA, 8. delfragmenten sannunbindes Ater kovalent, vilket sker enzymetiskt i det att man inkuberar en reaktionsblandning, innehallande DNA-ligas, ATP och de hada delfragmenten,t. ex. inkuberar namnda DNA-ligas, delfragment och ATP vid pH 7,6 och °C under 2 timmar, varvid man erhaller ett fragment av cDNA som kodar fir humant tillvaxthormon, och I) de ligasbehandlade cDNA fraktioneras alltefter sin langd, tax. medels, gelelektrofores, och man uppsamlar den fraktion, son innehaller ett for humant tillvaxrmon kodande eDNA-fragment, sem darigenom erhalhuvudsak fr_ran kontaminerande cDNA-sekvensel
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83621877A | 1977-09-23 | 1977-09-23 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8305163L true SE8305163L (sv) | 1983-09-23 |
| SE8305163D0 SE8305163D0 (sv) | 1983-09-23 |
Family
ID=25271469
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7806087A SE7806087L (sv) | 1977-09-23 | 1978-05-26 | Forfarande for rening av nukleotidsekvenser lempliga som syntetiseringsmall i bakterier, mikroorganism innefattande dylik nukleotidsekvens samt rekombinant dna-overforingsvektor innefattande dylik nukleotid |
| SE8305162A SE8305162D0 (sv) | 1977-09-23 | 1983-09-23 | Dna-overforingsvektor med korionsomatomammotropinkodoner, forfarande for dess framstellning, samt en nemnda vektor innehallande mikroorganism jemte dess framstellning |
| SE8305163A SE8305163D0 (sv) | 1977-09-23 | 1983-09-23 | Dna-overforingsvektor med tillvexthormonkodoner, forfarande for dess framstellning samt en nemnda vektor innehallande mikroorganism jemte dess framstellning |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7806087A SE7806087L (sv) | 1977-09-23 | 1978-05-26 | Forfarande for rening av nukleotidsekvenser lempliga som syntetiseringsmall i bakterier, mikroorganism innefattande dylik nukleotidsekvens samt rekombinant dna-overforingsvektor innefattande dylik nukleotid |
| SE8305162A SE8305162D0 (sv) | 1977-09-23 | 1983-09-23 | Dna-overforingsvektor med korionsomatomammotropinkodoner, forfarande for dess framstellning, samt en nemnda vektor innehallande mikroorganism jemte dess framstellning |
Country Status (25)
| Country | Link |
|---|---|
| JP (1) | JPS5448775A (sv) |
| AT (2) | AT369386B (sv) |
| AU (1) | AU519528B2 (sv) |
| BE (1) | BE868853A (sv) |
| CS (1) | CS235069B2 (sv) |
| DD (2) | DD153393A5 (sv) |
| DE (1) | DE2825595A1 (sv) |
| DK (1) | DK233378A (sv) |
| ES (1) | ES470340A1 (sv) |
| FI (1) | FI64187C (sv) |
| FR (1) | FR2404013A1 (sv) |
| GB (1) | GB1568047A (sv) |
| GR (1) | GR73558B (sv) |
| IE (1) | IE47332B1 (sv) |
| IL (1) | IL54791A (sv) |
| IT (1) | IT1098340B (sv) |
| LU (1) | LU79938A1 (sv) |
| NL (1) | NL7806358A (sv) |
| NZ (1) | NZ187342A (sv) |
| PL (1) | PL209748A1 (sv) |
| PT (1) | PT68127A (sv) |
| RO (2) | RO78407A (sv) |
| SE (3) | SE7806087L (sv) |
| YU (1) | YU214378A (sv) |
| ZA (1) | ZA782933B (sv) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RO80052B (ro) * | 1977-11-08 | 1985-03-30 | Genetech | Procedeu pentru prepararea unui vehicul recombinat de clonare pentru transformarea unei gazde bacteriene pentru a deveni generator de polipeptida heterologa |
| US6268122B1 (en) | 1978-12-22 | 2001-07-31 | Biogen, Inc. | Recombinant DNA molecules and their method of production |
| ZA802992B (en) * | 1979-06-01 | 1981-10-28 | Univ California | Human pre-growth hormone |
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| US4530901A (en) * | 1980-01-08 | 1985-07-23 | Biogen N.V. | Recombinant DNA molecules and their use in producing human interferon-like polypeptides |
| US6835557B1 (en) | 1980-01-08 | 2004-12-28 | Biogen, Inc. | DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides |
| IL59690A (en) | 1980-03-24 | 1983-11-30 | Yeda Res & Dev | Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it |
| ES8302096A1 (es) | 1980-04-03 | 1982-12-16 | Biogen Nv | Un metodo de producir un polipeptido que muestra una actividad immunologica obiologica de interferon de fibroblasto hu- mano. |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| CH659826A5 (fr) * | 1981-06-02 | 1987-02-27 | Wakunaga Yakuhin Kk | Procede d'obtention d'un microorganisme transforme par un plasmide comprenant un gene de structure de la 27-desamidosecretine. |
| US5670371A (en) * | 1983-07-15 | 1997-09-23 | Bio-Technology General Corp. | Bacterial expression of superoxide dismutase |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ187300A (en) * | 1977-05-27 | 1982-08-17 | Univ California | Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism |
| EP0001931A3 (en) * | 1977-11-08 | 1979-06-13 | Genentech, Inc. | Synthetic dna, process for preparing it and recombinant microbial cloning vehicle containing such dna |
-
1978
- 1978-05-22 ZA ZA00782933A patent/ZA782933B/xx unknown
- 1978-05-23 GR GR56310A patent/GR73558B/el unknown
- 1978-05-23 NZ NZ187342A patent/NZ187342A/xx unknown
- 1978-05-25 AU AU36489/78A patent/AU519528B2/en not_active Expired
- 1978-05-25 GB GB22418/78A patent/GB1568047A/en not_active Expired
- 1978-05-26 SE SE7806087A patent/SE7806087L/sv unknown
- 1978-05-26 DK DK233378A patent/DK233378A/da not_active Application Discontinuation
- 1978-05-26 FI FI781676A patent/FI64187C/fi not_active IP Right Cessation
- 1978-05-26 IL IL54791A patent/IL54791A/xx unknown
- 1978-05-26 IE IE1054/78A patent/IE47332B1/en unknown
- 1978-05-27 RO RO7894192A patent/RO78407A/ro unknown
- 1978-05-27 RO RO78105866A patent/RO80006A/ro unknown
- 1978-05-30 ES ES470340A patent/ES470340A1/es not_active Expired
- 1978-06-01 PT PT68127A patent/PT68127A/pt unknown
- 1978-06-05 JP JP6756678A patent/JPS5448775A/ja active Pending
- 1978-06-07 CS CS783704A patent/CS235069B2/cs unknown
- 1978-06-10 DE DE19782825595 patent/DE2825595A1/de not_active Withdrawn
- 1978-06-12 NL NL7806358A patent/NL7806358A/xx not_active Application Discontinuation
- 1978-06-16 IT IT24641/78A patent/IT1098340B/it active
- 1978-06-22 AT AT0456678A patent/AT369386B/de not_active IP Right Cessation
- 1978-07-03 DD DD78224374A patent/DD153393A5/de unknown
- 1978-07-03 DD DD78206482A patent/DD145917A5/de unknown
- 1978-07-04 FR FR7819901A patent/FR2404013A1/fr active Granted
- 1978-07-07 BE BE189169A patent/BE868853A/xx not_active IP Right Cessation
- 1978-07-07 LU LU79938A patent/LU79938A1/xx unknown
- 1978-09-11 YU YU02143/78A patent/YU214378A/xx unknown
- 1978-09-21 PL PL20974878A patent/PL209748A1/xx unknown
-
1981
- 1981-06-03 AT AT0248881A patent/AT375673B/de not_active IP Right Cessation
-
1983
- 1983-09-23 SE SE8305162A patent/SE8305162D0/sv not_active Application Discontinuation
- 1983-09-23 SE SE8305163A patent/SE8305163D0/sv not_active Application Discontinuation
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAV | Patent application has lapsed |
Ref document number: 8305163-1 |