DD153393A5 - Verfahren zur reinigung von nucleotidsequenzen - Google Patents
Verfahren zur reinigung von nucleotidsequenzen Download PDFInfo
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- DD153393A5 DD153393A5 DD78224374A DD22437478A DD153393A5 DD 153393 A5 DD153393 A5 DD 153393A5 DD 78224374 A DD78224374 A DD 78224374A DD 22437478 A DD22437478 A DD 22437478A DD 153393 A5 DD153393 A5 DD 153393A5
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- German Democratic Republic
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- dna
- sequence
- cdna
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- rna
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- 238000001042 affinity chromatography Methods 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000002942 anti-growth Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 208000017529 benign neoplasm of pituitary gland Diseases 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920000166 polytrimethylene carbonate Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 238000002601 radiography Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- MSFGZHUJTJBYFA-UHFFFAOYSA-M sodium dichloroisocyanurate Chemical compound [Na+].ClN1C(=O)[N-]C(=O)N(Cl)C1=O MSFGZHUJTJBYFA-UHFFFAOYSA-M 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000002480 thymidyl group Chemical group 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Pregnancy & Childbirth (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Diabetes (AREA)
- Bioinformatics & Computational Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US83621877A | 1977-09-23 | 1977-09-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
DD153393A5 true DD153393A5 (de) | 1982-01-06 |
Family
ID=25271469
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DD78224374A DD153393A5 (de) | 1977-09-23 | 1978-07-03 | Verfahren zur reinigung von nucleotidsequenzen |
DD78206482A DD145917A5 (de) | 1977-09-23 | 1978-07-03 | Verfahren zur reinigung von nucleo idsequenzen |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DD78206482A DD145917A5 (de) | 1977-09-23 | 1978-07-03 | Verfahren zur reinigung von nucleo idsequenzen |
Country Status (25)
Country | Link |
---|---|
JP (1) | JPS5448775A (sv) |
AT (2) | AT369386B (sv) |
AU (1) | AU519528B2 (sv) |
BE (1) | BE868853A (sv) |
CS (1) | CS235069B2 (sv) |
DD (2) | DD153393A5 (sv) |
DE (1) | DE2825595A1 (sv) |
DK (1) | DK233378A (sv) |
ES (1) | ES470340A1 (sv) |
FI (1) | FI64187C (sv) |
FR (1) | FR2404013A1 (sv) |
GB (1) | GB1568047A (sv) |
GR (1) | GR73558B (sv) |
IE (1) | IE47332B1 (sv) |
IL (1) | IL54791A (sv) |
IT (1) | IT1098340B (sv) |
LU (1) | LU79938A1 (sv) |
NL (1) | NL7806358A (sv) |
NZ (1) | NZ187342A (sv) |
PL (1) | PL209748A1 (sv) |
PT (1) | PT68127A (sv) |
RO (2) | RO78407A (sv) |
SE (3) | SE7806087L (sv) |
YU (1) | YU214378A (sv) |
ZA (1) | ZA782933B (sv) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RO80052B (ro) * | 1977-11-08 | 1985-03-30 | Genetech | Procedeu pentru prepararea unui vehicul recombinat de clonare pentru transformarea unei gazde bacteriene pentru a deveni generator de polipeptida heterologa |
US6268122B1 (en) | 1978-12-22 | 2001-07-31 | Biogen, Inc. | Recombinant DNA molecules and their method of production |
ZA802992B (en) * | 1979-06-01 | 1981-10-28 | Univ California | Human pre-growth hormone |
US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
US6835557B1 (en) | 1980-01-08 | 2004-12-28 | Biogen, Inc. | DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides |
US4530901A (en) * | 1980-01-08 | 1985-07-23 | Biogen N.V. | Recombinant DNA molecules and their use in producing human interferon-like polypeptides |
IL59690A (en) | 1980-03-24 | 1983-11-30 | Yeda Res & Dev | Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it |
ATE56471T1 (de) | 1980-04-03 | 1990-09-15 | Biogen Inc | Dns-sequenzen, rekombinante dns-molekuele und verfahren zur herstellung von dem menschlichen fibroblast-interferon. |
US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
CH659826A5 (fr) * | 1981-06-02 | 1987-02-27 | Wakunaga Yakuhin Kk | Procede d'obtention d'un microorganisme transforme par un plasmide comprenant un gene de structure de la 27-desamidosecretine. |
US5670371A (en) * | 1983-07-15 | 1997-09-23 | Bio-Technology General Corp. | Bacterial expression of superoxide dismutase |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ187300A (en) * | 1977-05-27 | 1982-08-17 | Univ California | Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism |
CA1201668A (en) * | 1977-11-08 | 1986-03-11 | Genentech, Inc. | Synthetic dna and method therefor |
-
1978
- 1978-05-22 ZA ZA00782933A patent/ZA782933B/xx unknown
- 1978-05-23 NZ NZ187342A patent/NZ187342A/xx unknown
- 1978-05-23 GR GR56310A patent/GR73558B/el unknown
- 1978-05-25 AU AU36489/78A patent/AU519528B2/en not_active Expired
- 1978-05-25 GB GB22418/78A patent/GB1568047A/en not_active Expired
- 1978-05-26 SE SE7806087A patent/SE7806087L/sv unknown
- 1978-05-26 DK DK233378A patent/DK233378A/da not_active Application Discontinuation
- 1978-05-26 IL IL54791A patent/IL54791A/xx unknown
- 1978-05-26 FI FI781676A patent/FI64187C/fi not_active IP Right Cessation
- 1978-05-26 IE IE1054/78A patent/IE47332B1/en unknown
- 1978-05-27 RO RO7894192A patent/RO78407A/ro unknown
- 1978-05-27 RO RO78105866A patent/RO80006A/ro unknown
- 1978-05-30 ES ES470340A patent/ES470340A1/es not_active Expired
- 1978-06-01 PT PT68127A patent/PT68127A/pt unknown
- 1978-06-05 JP JP6756678A patent/JPS5448775A/ja active Pending
- 1978-06-07 CS CS783704A patent/CS235069B2/cs unknown
- 1978-06-10 DE DE19782825595 patent/DE2825595A1/de not_active Withdrawn
- 1978-06-12 NL NL7806358A patent/NL7806358A/xx not_active Application Discontinuation
- 1978-06-16 IT IT24641/78A patent/IT1098340B/it active
- 1978-06-22 AT AT0456678A patent/AT369386B/de not_active IP Right Cessation
- 1978-07-03 DD DD78224374A patent/DD153393A5/de unknown
- 1978-07-03 DD DD78206482A patent/DD145917A5/de unknown
- 1978-07-04 FR FR7819901A patent/FR2404013A1/fr active Granted
- 1978-07-07 LU LU79938A patent/LU79938A1/xx unknown
- 1978-07-07 BE BE189169A patent/BE868853A/xx not_active IP Right Cessation
- 1978-09-11 YU YU02143/78A patent/YU214378A/xx unknown
- 1978-09-21 PL PL20974878A patent/PL209748A1/xx unknown
-
1981
- 1981-06-03 AT AT0248881A patent/AT375673B/de not_active IP Right Cessation
-
1983
- 1983-09-23 SE SE8305162A patent/SE8305162D0/sv not_active Application Discontinuation
- 1983-09-23 SE SE8305163A patent/SE8305163D0/sv not_active Application Discontinuation
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