SE8305162L - Dna-overforingsvektor med korionsomatomammotropinkodoner, forfarande for dess framstellning, samt en nemnda vektor innehallande mikroorganism jemte dess framstellning - Google Patents
Dna-overforingsvektor med korionsomatomammotropinkodoner, forfarande for dess framstellning, samt en nemnda vektor innehallande mikroorganism jemte dess framstellningInfo
- Publication number
- SE8305162L SE8305162L SE8305162A SE8305162A SE8305162L SE 8305162 L SE8305162 L SE 8305162L SE 8305162 A SE8305162 A SE 8305162A SE 8305162 A SE8305162 A SE 8305162A SE 8305162 L SE8305162 L SE 8305162L
- Authority
- SE
- Sweden
- Prior art keywords
- cdna
- fragments
- population
- fragment
- encoding
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 3
- 230000005540 biological transmission Effects 0.000 title 1
- 244000005700 microbiome Species 0.000 title 1
- 239000012634 fragment Substances 0.000 claims 20
- 239000002299 complementary DNA Substances 0.000 claims 16
- 108020004635 Complementary DNA Proteins 0.000 claims 3
- 238000001502 gel electrophoresis Methods 0.000 claims 3
- 238000011534 incubation Methods 0.000 claims 3
- 239000011541 reaction mixture Substances 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- 102000012410 DNA Ligases Human genes 0.000 claims 2
- 108010061982 DNA Ligases Proteins 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 210000005059 placental tissue Anatomy 0.000 claims 2
- 108091008146 restriction endonucleases Proteins 0.000 claims 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 1
- 241000272814 Anser sp. Species 0.000 claims 1
- 102100031780 Endonuclease Human genes 0.000 claims 1
- 208000034454 F12-related hereditary angioedema with normal C1Inh Diseases 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 208000016861 hereditary angioedema type 3 Diseases 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 230000000087 stabilizing effect Effects 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Diabetes (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Pregnancy & Childbirth (AREA)
- Physics & Mathematics (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (9)
1. Patentkrav Harfarande for framstallning av en averfaringsvektor, son bar en for humant korionsOmatomammotropin kodande nukleotidsekvens, varvid man isolerar vavnad fran human placenta, isolerar en mlINA-population ur denna placentara vavnad, syntetiserar en cnNA-population utgaende fran namnda mRNA, ur denna sistnamnda population renar cDNA kodande for humant korionscmatomammotropin och bringar denna renade cDNA art reagera mad en DNA-overfaringsvektor, och varvid namnda renade cDNA utgor en mindre andel i namnda cDNA-population, atminstone en del as cDNA-molekylerna bar minst tv5 restriktionsstallen, och mannda °DNA-population Pr heterogen i fraga om langd och sekvens och framstalles utgaende fran namnda, i frAga cm langd och sekvens heterogena mRNA-population medelst en med reverstranskriptas katalyserad teaktion,kannetecknat av alt namnda far humant korionsomatomammotropin kodande cDNA renas medelst ett forfarande som innefattar foljande stag: a) cDNA-populationen hydrolyseras enzymatiskt specifikt vid namn- da restriktionsstallenlipm inkubationen reaktionsblandning, in- nehallande cONA-populan och ett faas restriktionsstallen specifkt restriktionsendonukleas, tex. inkubation av namnda popula- tion med HaeIII vid pH 7,5 och 37°C i 1-2 timer, till bildning as fragment as de i populationen ingaende cDNA,
2. dense cDNA-fragment fraktioneras alltefter sin langd, t.ex. genom gelelektrofores, varigenom dessa cDNA-fragment uppdelas i frak. tioner, vilka innehalla i frAga om langden homogena cDNA-fragment,
3. man uppsamlar den fraktion, corn innehaller det far humant korionsomatomammotropin kodande oDNA-fragmentet, varigenom namnda fraktion kommer alt till Overvagande del innehalla namnda fragment, saint eventuellt ytterligare rening as den fir humant korionsomatomammotropin kodande cDNA ur de i frAga om langden homogena cDNA-fragment, som till Overvagande del innehaller cDNA kodande far humant koricnsomatomammotropin, utfbres medelst en process son innefattar foljande ytterligare steg:
4. alla ev. --fosfatandgrupper pa cONA-fragmenten hydrolyseras, t.ex. genom att fragmenten inkuberas med alkaliskt fosfatas vid pH 7,5 och 60°C under 10 minuter,
5. namnda eDNA-fragment hydrolyseras enzymatiskt specifikt vid namnda restriktionsstalle goose inkubation av en reaktionsblandning, son innehaller namnda fragment och ett fir delta restriktionsstalle specifikt restriktionsendonukleas, t.ex. inkubation so namnda fragment med Shah eller HpaII vid pH 7,6 och 37°C under 2 timmar, varvid delfragment bildas, son utgores as delar av namnda fragment,
6. delfragmenten fraktioneras alltefter sin langcl, t . ex . medal st gelelektrofores,
7. man uppsamlar is fraktioner, som innehaller de bada fir humeet korionsomatomammotropin kodande delfragmenten av namnda cDNA,
8. delfragmenten smmanbindes Ater kovalent, vilket sker enzymatiskt i Oct alt man inkuberar en reaktionsblandning, innenallande DNA-ligas, ATP och de bada delfragmenten, t.ex. inkuberar namnda DNA-ligas, delfragment och ATP vid pH 7,6 och °C under 2 timmar, varvid man erhAller ett fragment av cDNA sum kodar fir humant. korionsomatomammotropin, och
9. de ligasbehandlade cDNA fraktioneras alltefter sin langd, t.ex. medelst gelelektrofores, och man uppsamlar den fraktion, son innehAller ett fir humant korionsonatomammotropin kodande cDNA- fragment, sonenom entAilhuvudsak fritt fran kontamine- rande cDNA-setsr.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83621877A | 1977-09-23 | 1977-09-23 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8305162L true SE8305162L (sv) | 1983-09-23 |
| SE8305162D0 SE8305162D0 (sv) | 1983-09-23 |
Family
ID=25271469
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7806087A SE7806087L (sv) | 1977-09-23 | 1978-05-26 | Forfarande for rening av nukleotidsekvenser lempliga som syntetiseringsmall i bakterier, mikroorganism innefattande dylik nukleotidsekvens samt rekombinant dna-overforingsvektor innefattande dylik nukleotid |
| SE8305163A SE8305163D0 (sv) | 1977-09-23 | 1983-09-23 | Dna-overforingsvektor med tillvexthormonkodoner, forfarande for dess framstellning samt en nemnda vektor innehallande mikroorganism jemte dess framstellning |
| SE8305162A SE8305162D0 (sv) | 1977-09-23 | 1983-09-23 | Dna-overforingsvektor med korionsomatomammotropinkodoner, forfarande for dess framstellning, samt en nemnda vektor innehallande mikroorganism jemte dess framstellning |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7806087A SE7806087L (sv) | 1977-09-23 | 1978-05-26 | Forfarande for rening av nukleotidsekvenser lempliga som syntetiseringsmall i bakterier, mikroorganism innefattande dylik nukleotidsekvens samt rekombinant dna-overforingsvektor innefattande dylik nukleotid |
| SE8305163A SE8305163D0 (sv) | 1977-09-23 | 1983-09-23 | Dna-overforingsvektor med tillvexthormonkodoner, forfarande for dess framstellning samt en nemnda vektor innehallande mikroorganism jemte dess framstellning |
Country Status (25)
| Country | Link |
|---|---|
| JP (1) | JPS5448775A (sv) |
| AT (2) | AT369386B (sv) |
| AU (1) | AU519528B2 (sv) |
| BE (1) | BE868853A (sv) |
| CS (1) | CS235069B2 (sv) |
| DD (2) | DD153393A5 (sv) |
| DE (1) | DE2825595A1 (sv) |
| DK (1) | DK233378A (sv) |
| ES (1) | ES470340A1 (sv) |
| FI (1) | FI64187C (sv) |
| FR (1) | FR2404013A1 (sv) |
| GB (1) | GB1568047A (sv) |
| GR (1) | GR73558B (sv) |
| IE (1) | IE47332B1 (sv) |
| IL (1) | IL54791A (sv) |
| IT (1) | IT1098340B (sv) |
| LU (1) | LU79938A1 (sv) |
| NL (1) | NL7806358A (sv) |
| NZ (1) | NZ187342A (sv) |
| PL (1) | PL209748A1 (sv) |
| PT (1) | PT68127A (sv) |
| RO (2) | RO78407A (sv) |
| SE (3) | SE7806087L (sv) |
| YU (1) | YU214378A (sv) |
| ZA (1) | ZA782933B (sv) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR7807290A (pt) * | 1977-11-08 | 1979-06-12 | Genentech Inc | Plasmideo,processo para producao de um polipeptido especifico veiculo clonal,cultura bacteriana transformante,processo para produzir uma substancia imunogenica processo para preparar um gene estrutural,e polidesoxirribonucleotido de faixa dupla |
| US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
| ZA802992B (en) * | 1979-06-01 | 1981-10-28 | Univ California | Human pre-growth hormone |
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| US6835557B1 (en) | 1980-01-08 | 2004-12-28 | Biogen, Inc. | DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides |
| US4530901A (en) * | 1980-01-08 | 1985-07-23 | Biogen N.V. | Recombinant DNA molecules and their use in producing human interferon-like polypeptides |
| IL59690A (en) | 1980-03-24 | 1983-11-30 | Yeda Res & Dev | Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it |
| DE3177213D1 (de) | 1980-04-03 | 1990-10-18 | Biogen Inc | Dns-sequenzen, rekombinante dns-molekuele und verfahren zur herstellung von dem menschlichen fibroblast-interferon. |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| CH659826A5 (fr) * | 1981-06-02 | 1987-02-27 | Wakunaga Yakuhin Kk | Procede d'obtention d'un microorganisme transforme par un plasmide comprenant un gene de structure de la 27-desamidosecretine. |
| US5670371A (en) * | 1983-07-15 | 1997-09-23 | Bio-Technology General Corp. | Bacterial expression of superoxide dismutase |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ187300A (en) * | 1977-05-27 | 1982-08-17 | Univ California | Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism |
| IE47889B1 (en) * | 1977-11-08 | 1984-07-11 | Genentech Inc | Synthetic dna and process tehrefor |
-
1978
- 1978-05-22 ZA ZA00782933A patent/ZA782933B/xx unknown
- 1978-05-23 NZ NZ187342A patent/NZ187342A/xx unknown
- 1978-05-23 GR GR56310A patent/GR73558B/el unknown
- 1978-05-25 GB GB22418/78A patent/GB1568047A/en not_active Expired
- 1978-05-25 AU AU36489/78A patent/AU519528B2/en not_active Expired
- 1978-05-26 DK DK233378A patent/DK233378A/da not_active Application Discontinuation
- 1978-05-26 IL IL54791A patent/IL54791A/xx unknown
- 1978-05-26 FI FI781676A patent/FI64187C/fi not_active IP Right Cessation
- 1978-05-26 IE IE1054/78A patent/IE47332B1/en unknown
- 1978-05-26 SE SE7806087A patent/SE7806087L/sv unknown
- 1978-05-27 RO RO7894192A patent/RO78407A/ro unknown
- 1978-05-27 RO RO78105866A patent/RO80006A/ro unknown
- 1978-05-30 ES ES470340A patent/ES470340A1/es not_active Expired
- 1978-06-01 PT PT68127A patent/PT68127A/pt unknown
- 1978-06-05 JP JP6756678A patent/JPS5448775A/ja active Pending
- 1978-06-07 CS CS783704A patent/CS235069B2/cs unknown
- 1978-06-10 DE DE19782825595 patent/DE2825595A1/de not_active Withdrawn
- 1978-06-12 NL NL7806358A patent/NL7806358A/xx not_active Application Discontinuation
- 1978-06-16 IT IT24641/78A patent/IT1098340B/it active
- 1978-06-22 AT AT0456678A patent/AT369386B/de not_active IP Right Cessation
- 1978-07-03 DD DD78224374A patent/DD153393A5/de unknown
- 1978-07-03 DD DD78206482A patent/DD145917A5/de unknown
- 1978-07-04 FR FR7819901A patent/FR2404013A1/fr active Granted
- 1978-07-07 BE BE189169A patent/BE868853A/xx not_active IP Right Cessation
- 1978-07-07 LU LU79938A patent/LU79938A1/xx unknown
- 1978-09-11 YU YU02143/78A patent/YU214378A/xx unknown
- 1978-09-21 PL PL20974878A patent/PL209748A1/xx unknown
-
1981
- 1981-06-03 AT AT0248881A patent/AT375673B/de not_active IP Right Cessation
-
1983
- 1983-09-23 SE SE8305163A patent/SE8305163D0/sv not_active Application Discontinuation
- 1983-09-23 SE SE8305162A patent/SE8305162D0/sv not_active Application Discontinuation
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