SE8305163L - DNA TRANSMISSION VECTOR WITH GROWTH HORMON CODONES, PROCEDURE FOR ITS MANUFACTURING, AND A MENTIONED VECTOR INCLUDING MICROORGANISM AND ITS MANUFACTURING - Google Patents

DNA TRANSMISSION VECTOR WITH GROWTH HORMON CODONES, PROCEDURE FOR ITS MANUFACTURING, AND A MENTIONED VECTOR INCLUDING MICROORGANISM AND ITS MANUFACTURING

Info

Publication number
SE8305163L
SE8305163L SE8305163A SE8305163A SE8305163L SE 8305163 L SE8305163 L SE 8305163L SE 8305163 A SE8305163 A SE 8305163A SE 8305163 A SE8305163 A SE 8305163A SE 8305163 L SE8305163 L SE 8305163L
Authority
SE
Sweden
Prior art keywords
cdna
fragments
population
fragment
sub
Prior art date
Application number
SE8305163A
Other languages
Swedish (sv)
Other versions
SE8305163D0 (en
Inventor
H M Goodman
P H Seeburg
J Shine
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of SE8305163L publication Critical patent/SE8305163L/en
Publication of SE8305163D0 publication Critical patent/SE8305163D0/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57518—Placental lactogen; Chorionic somatomammotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Diabetes (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (1)

Patentkrav Ffirfarande fir framstallning as en overforingsvektor, son har on fOr humant tillvaxformon kodande nukleotidsekvens, varvid man Lsolerar vavnad fran human hypofys, isolerar en mRNA-population or donna hypofysvavnad, syntetiserar en cDNA-population utgaende fran namnda mANA, ur derma sistnamnda population renar cDNA kodande fir humant tillvaxthormon och bringer denna renade cDNA att reagera med en DNA-overfOringsvektor, och varvid namnda renade oDNA utgar en mindre andel i namnda cDNA-population, itminstone en del av cDNA-molekylerna har minst tub restriktionsstallen, och namnda cDNA-population Or heterogen i fraga om langd och sekvens och framstalles utgiende frin namnda,i fraga on langd och sekvens heterogena mRNA-populationnedelst en med reverstranskriptas katalyserad reaktion,kannetecknat a v att naranda fir humant tillvaxthormon kodande cDNA renas medelst ett forfarande som innefattar foljande steq: al cDNA-populationen hydrolyseras enzymatiskt specifikt vid namda restriktionsstallen genom inkubation aven reaktionsblandning, innehallande cDNA-poionen och et. dessa restriktionsstallen specifikt restriktiononukleas, t.nkubation av namnda popu- t-A method for producing as a transfer vector, which has a nucleotide sequence encoding human growth form, isolating tissue from human pituitary gland, isolating an mRNA population or donor pituitary tissue, synthesizing a cDNA population from the latter cANNA, encoding human growth hormone and causing this purified cDNA to react with a DNA transfer vector, wherein said purified oDNA emits a smaller proportion in said cDNA population, at least some of the cDNA molecules have at least the tube restriction rate, and said cDNA population Or heterogeneous in length and sequence and is prepared starting from the said, in length and sequence heterogeneous mRNA population by a reverse transcriptase catalyzed reaction, characterized in that naranda for human growth hormone encoding cDNA is purified by a method comprising the following steq: al cDNA the population is enzymatically hydrolyzed specifically at namda restri the incubation site by incubating a reaction mixture containing the cDNA pion and et. these restriction sites specifically restriction onuclease, incubation of said population. 1. -d) ails ev. 5'-fosfat5ndgrupper pi cDNA-fragmenten hydrolyseras, ten. genom att fragmenten inkuberas med alkaliskt fosfatas vid pH 7,5 och 60°C under 10 minuter, el namnda cDNA-fragment hydrolyseras enzymatiskt specifikt vid namnda restriktionsst011e genom :lnkubation av en reaktionsblandning, som innehaller namnda fragment och ett fir detta restriktionsstalle specifikt restriktionsendonukleas, t.ex. inkubation av namnda fragment med PvuIT vid pH 7,6 och 37°C under 2 timmar, varvid delfragment bildas, son utgOres av delar av namnda fragment, 6. delfragmenten fraktioneras alltefter sin langd, t.ex. medelst gelelektrofores, 7. man uppsamlar de fraktoner, son innehiller de bade fir humant tillvaxthormon kodande delfragmenten av namnda cDNA, 8. delfragmenten sannunbindes Ater kovalent, vilket sker enzymetiskt i det att man inkuberar en reaktionsblandning, innehallande DNA-ligas, ATP och de hada delfragmenten,t. ex. inkuberar namnda DNA-ligas, delfragment och ATP vid pH 7,6 och °C under 2 timmar, varvid man erhaller ett fragment av cDNA som kodar fir humant tillvaxthormon, och I) de ligasbehandlade cDNA fraktioneras alltefter sin langd, tax. medels, gelelektrofores, och man uppsamlar den fraktion, son innehaller ett for humant tillvaxrmon kodande eDNA-fragment, sem darigenom erhalhuvudsak fr_ran kontaminerande cDNA-sekvensel1. -d) ails ev. 5 'phosphate end groups in the cDNA fragments are hydrolyzed. by incubating the fragments with alkaline phosphatase at pH 7.5 and 60 ° C for 10 minutes, or said cDNA fragment is enzymatically hydrolyzed specifically at said restriction site by: incubating a reaction mixture containing said fragment and a restriction endonuclease specific for that restriction site. for example incubation of said fragment with PvuIT at pH 7.6 and 37 ° C for 2 hours, forming sub-fragments, consisting of parts of said fragment, 6. the sub-fragments are fractionated according to their length, e.g. by gel electrophoresis, 7. the fractons are collected, which contain both human growth hormone-encoding sub-fragments of said cDNA, 8. the sub-fragments are covalently bound again, which is done enzymatically by incubating a reaction mixture containing DNA ligase, ATP and de had ligase sub-fragments, t. ex. incubates said DNA ligase, sub-fragments and ATP at pH 7.6 and ° C for 2 hours, obtaining a fragment of cDNA encoding human growth hormone, and I) the ligase-treated cDNAs are fractionated according to their length, dachshund. gel electrophoresis, and the fraction containing a human growth coding eDNA fragment is collected, thereby obtaining mainly from contaminating cDNA sequences.
SE8305163A 1977-09-23 1983-09-23 DNA TRANSMISSION VECTOR WITH GROWTH HORMON CODONES, PROCEDURE FOR ITS MANUFACTURING, AND A MENTIONED VECTOR INCLUDING MICROORGANISM AND ITS MANUFACTURING SE8305163D0 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US83621877A 1977-09-23 1977-09-23

Publications (2)

Publication Number Publication Date
SE8305163L true SE8305163L (en) 1983-09-23
SE8305163D0 SE8305163D0 (en) 1983-09-23

Family

ID=25271469

Family Applications (3)

Application Number Title Priority Date Filing Date
SE7806087A SE7806087L (en) 1977-09-23 1978-05-26 PROCEDURE FOR PURIFICATION OF NUCLEOTIDE SEQUENCES SUITABLE AS SYNTHETIZATION TEMPLATE IN BACTERIA, MICRO-ORGANISM INCLUDING DYLIC NUCLEOTIDE SEQUENCE AND RECOMBINANT DNA TRANSFER VECTOR INNECIDANT
SE8305163A SE8305163D0 (en) 1977-09-23 1983-09-23 DNA TRANSMISSION VECTOR WITH GROWTH HORMON CODONES, PROCEDURE FOR ITS MANUFACTURING, AND A MENTIONED VECTOR INCLUDING MICROORGANISM AND ITS MANUFACTURING
SE8305162A SE8305162D0 (en) 1977-09-23 1983-09-23 DNA TRANSMISSION VECTOR WITH CORION ATOMMOTROPIN CODONES, PROCEDURE FOR ITS PROCESSING, AND A SAFETY VECTOR CONTAINING MICROORGANISM BECAUSE ITS PREPARATION

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SE7806087A SE7806087L (en) 1977-09-23 1978-05-26 PROCEDURE FOR PURIFICATION OF NUCLEOTIDE SEQUENCES SUITABLE AS SYNTHETIZATION TEMPLATE IN BACTERIA, MICRO-ORGANISM INCLUDING DYLIC NUCLEOTIDE SEQUENCE AND RECOMBINANT DNA TRANSFER VECTOR INNECIDANT

Family Applications After (1)

Application Number Title Priority Date Filing Date
SE8305162A SE8305162D0 (en) 1977-09-23 1983-09-23 DNA TRANSMISSION VECTOR WITH CORION ATOMMOTROPIN CODONES, PROCEDURE FOR ITS PROCESSING, AND A SAFETY VECTOR CONTAINING MICROORGANISM BECAUSE ITS PREPARATION

Country Status (25)

Country Link
JP (1) JPS5448775A (en)
AT (2) AT369386B (en)
AU (1) AU519528B2 (en)
BE (1) BE868853A (en)
CS (1) CS235069B2 (en)
DD (2) DD153393A5 (en)
DE (1) DE2825595A1 (en)
DK (1) DK233378A (en)
ES (1) ES470340A1 (en)
FI (1) FI64187C (en)
FR (1) FR2404013A1 (en)
GB (1) GB1568047A (en)
GR (1) GR73558B (en)
IE (1) IE47332B1 (en)
IL (1) IL54791A (en)
IT (1) IT1098340B (en)
LU (1) LU79938A1 (en)
NL (1) NL7806358A (en)
NZ (1) NZ187342A (en)
PL (1) PL209748A1 (en)
PT (1) PT68127A (en)
RO (2) RO78407A (en)
SE (3) SE7806087L (en)
YU (1) YU214378A (en)
ZA (1) ZA782933B (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR7807290A (en) * 1977-11-08 1979-06-12 Genentech Inc PLASMIDEO, PROCESS FOR THE PRODUCTION OF A SPECIFIC POLYPEPTIDE CLONAL VEHICLE, TRANSFORMING BACTERIAL CULTURE, PROCESS TO PRODUCE AN IMMUNOGENIC SUBSTANCE, PROCESS TO PREPARE A STRUCTURAL, AND POLYDESOXIRRIBONUCLEOTIDE GENE
US6297355B1 (en) 1978-12-22 2001-10-02 Biogen, Inc. Polypeptides displaying HBV antigenicity or hbv antigen specificity
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US6835557B1 (en) 1980-01-08 2004-12-28 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides
US4530901A (en) * 1980-01-08 1985-07-23 Biogen N.V. Recombinant DNA molecules and their use in producing human interferon-like polypeptides
IL59690A (en) 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it
DE3177213D1 (en) 1980-04-03 1990-10-18 Biogen Inc DNA SEQUENCES, RECOMBINANT DNA MOLECULES AND METHOD FOR PRODUCING THE HUMAN FIBROBLAST INTERFERON.
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
CH659826A5 (en) * 1981-06-02 1987-02-27 Wakunaga Yakuhin Kk PROCESS FOR OBTAINING A MICROORGANISM TRANSFORMED BY A PLASMID COMPRISING A GENE OF 27-DESAMIDOSECRETINE STRUCTURE.
US5670371A (en) * 1983-07-15 1997-09-23 Bio-Technology General Corp. Bacterial expression of superoxide dismutase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ187300A (en) * 1977-05-27 1982-08-17 Univ California Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism
IE47889B1 (en) * 1977-11-08 1984-07-11 Genentech Inc Synthetic dna and process tehrefor

Also Published As

Publication number Publication date
AT375673B (en) 1984-08-27
AU3648978A (en) 1979-11-29
FI64187C (en) 1983-10-10
IE47332B1 (en) 1984-02-22
FI64187B (en) 1983-06-30
ZA782933B (en) 1979-05-30
DE2825595A1 (en) 1979-04-12
PL209748A1 (en) 1979-12-03
CS235069B2 (en) 1985-04-16
SE8305162L (en) 1983-09-23
RO78407A (en) 1982-02-26
IT1098340B (en) 1985-09-07
DK233378A (en) 1979-03-24
ATA456678A (en) 1982-05-15
DD145917A5 (en) 1981-01-14
DD153393A5 (en) 1982-01-06
FI781676A (en) 1979-03-24
IE781054L (en) 1979-03-23
SE8305162D0 (en) 1983-09-23
SE8305163D0 (en) 1983-09-23
AT369386B (en) 1982-12-27
PT68127A (en) 1978-07-01
ATA248881A (en) 1984-01-15
NZ187342A (en) 1983-09-02
IL54791A (en) 1983-07-31
RO80006A (en) 1982-10-11
NL7806358A (en) 1979-03-27
ES470340A1 (en) 1979-09-16
LU79938A1 (en) 1978-12-12
AU519528B2 (en) 1981-12-10
SE7806087L (en) 1979-03-24
YU214378A (en) 1984-04-30
IT7824641A0 (en) 1978-06-16
GR73558B (en) 1984-03-15
JPS5448775A (en) 1979-04-17
FR2404013A1 (en) 1979-04-20
GB1568047A (en) 1980-05-21
FR2404013B1 (en) 1983-07-22
BE868853A (en) 1978-11-03

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