DE2459218C3 - Vorrichtung zum Transferieren eines tiefgekühlten Präparatschnittes von einem Mikrotom o.dgl. zu einem Mikroskop, insbesondere Elektronenmikroskop - Google Patents
Vorrichtung zum Transferieren eines tiefgekühlten Präparatschnittes von einem Mikrotom o.dgl. zu einem Mikroskop, insbesondere ElektronenmikroskopInfo
- Publication number
- DE2459218C3 DE2459218C3 DE2459218A DE2459218A DE2459218C3 DE 2459218 C3 DE2459218 C3 DE 2459218C3 DE 2459218 A DE2459218 A DE 2459218A DE 2459218 A DE2459218 A DE 2459218A DE 2459218 C3 DE2459218 C3 DE 2459218C3
- Authority
- DE
- Germany
- Prior art keywords
- transfer container
- cylinder
- vacuum lock
- sleeve
- microscope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000012546 transfer Methods 0.000 claims description 55
- 238000001816 cooling Methods 0.000 claims description 18
- 238000010438 heat treatment Methods 0.000 claims description 11
- 239000002826 coolant Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 5
- 239000004020 conductor Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 2
- 229910052782 aluminium Inorganic materials 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 239000011810 insulating material Substances 0.000 claims description 2
- 239000012212 insulator Substances 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000000110 cooling liquid Substances 0.000 description 5
- 238000009833 condensation Methods 0.000 description 4
- 230000005494 condensation Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000001493 electron microscopy Methods 0.000 description 2
- 238000005530 etching Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000078511 Microtome Species 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000003197 gene knockdown Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
- H01J37/02—Details
- H01J37/18—Vacuum locks ; Means for obtaining or maintaining the desired pressure within the vessel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S62/00—Refrigeration
- Y10S62/01—Radiant cooling
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Microscoopes, Condenser (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT265374 | 1974-03-29 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2459218A1 DE2459218A1 (de) | 1975-10-09 |
| DE2459218B2 DE2459218B2 (de) | 1977-12-29 |
| DE2459218C3 true DE2459218C3 (de) | 1978-09-07 |
Family
ID=3538137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2459218A Expired DE2459218C3 (de) | 1974-03-29 | 1974-12-14 | Vorrichtung zum Transferieren eines tiefgekühlten Präparatschnittes von einem Mikrotom o.dgl. zu einem Mikroskop, insbesondere Elektronenmikroskop |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US3978686A (enExample) |
| JP (1) | JPS585505B2 (enExample) |
| DE (1) | DE2459218C3 (enExample) |
| SE (1) | SE399133B (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT343941B (de) * | 1976-02-06 | 1978-06-26 | Reichert Optische Werke Ag | Verfahren und vorrichtung zur herstellung von praparaten fur die mikroskopie, insbesondere elektronenmikroskopie aus nativen histologischen objekten sowie physikochemisch ahnlichen produkten durch kryofixation |
| DE2944806A1 (de) * | 1979-11-06 | 1981-05-14 | C. Reichert Optische Werke Ag, Wien | Einrichtung zur metallspiegel-kryofixation sowie zur nachfolgenden kryopraeparation biologischer objekte |
| DE3145505A1 (de) * | 1981-11-17 | 1983-05-26 | C. Reichert Optische Werke AG, 1170 Wien | Transfereinrichtung zur entnahme von gefriergetrockneten schnitten aus der kuehlkammer eines gefriermikrotomes, insbesondere eines kryo-ultramikrotomes |
| US4408464A (en) * | 1982-03-23 | 1983-10-11 | The United States Of America As Represented By The Secretary Of The Air Force | Dewar cooling chamber for semiconductor platelets |
| US4395075A (en) * | 1982-03-24 | 1983-07-26 | E. I. Du Pont De Nemours & Co. | Misalignment system for a microtome |
| US4546614A (en) * | 1984-04-13 | 1985-10-15 | General Dynamics Pomona Division | Precooled detector leads |
| US4663944A (en) * | 1985-07-12 | 1987-05-12 | Cornell Research Foundation, Inc. | Cryogenic sample stage for an ion microscope |
| US4869077A (en) * | 1987-08-21 | 1989-09-26 | Hypres, Inc. | Open-cycle cooling apparatus |
| DE8814284U1 (de) * | 1988-11-15 | 1989-01-19 | Slee Technik GmbH, 6500 Mainz | Gerät zum Kaltschneiden von Gewebeproben u.dgl. |
| US5050470A (en) * | 1989-05-15 | 1991-09-24 | Ward John L | Receiver for specimens from cryogenic microtome |
| US6387653B1 (en) | 1999-04-09 | 2002-05-14 | Culterra, Llc | Apparatus and method for automatically producing tissue slides |
| US7053370B2 (en) * | 2001-10-05 | 2006-05-30 | Canon Kabushiki Kaisha | Information acquisition apparatus, cross section evaluating apparatus, cross section evaluating method, and cross section working apparatus |
| JP3872709B2 (ja) * | 2002-04-01 | 2007-01-24 | サクラ精機株式会社 | 組織切片の作製方法及びその作製装置 |
| JP2004227842A (ja) * | 2003-01-21 | 2004-08-12 | Canon Inc | プローブ保持装置、試料の取得装置、試料加工装置、試料加工方法、および試料評価方法 |
| JP2005062130A (ja) * | 2003-08-20 | 2005-03-10 | Canon Inc | 微小薄片作製装置 |
| US20060175014A1 (en) * | 2005-02-10 | 2006-08-10 | Michael Cox | Specimen surface treatment system |
| US20060175291A1 (en) * | 2005-02-10 | 2006-08-10 | Hunt John A | Control of process gases in specimen surface treatment system |
| US20060175013A1 (en) * | 2005-02-10 | 2006-08-10 | Michael Cox | Specimen surface treatment system |
| JP4840763B2 (ja) * | 2006-01-18 | 2011-12-21 | セイコーインスツル株式会社 | 自動薄切片作製装置及び自動薄切片標本作製装置 |
| KR101440571B1 (ko) * | 2012-07-05 | 2014-09-17 | 광주과학기술원 | 세포 이미징 방법 및 장치 |
| CN114740029A (zh) * | 2022-04-08 | 2022-07-12 | 北京大学 | 一种传送装置和包括该传送装置的无污染实验系统 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3066222A (en) * | 1959-11-18 | 1962-11-27 | Union Carbide Corp | Infra-red detection apparatus |
| US3296825A (en) * | 1964-11-02 | 1967-01-10 | Gen Electric | Solid state electronic device and method |
| US3332254A (en) * | 1965-08-16 | 1967-07-25 | Elovic Ernest | Heater for the maintenance of stable two-phase flow in uninsulated, flexible-transfer-line cryogenic systems |
| US3327491A (en) * | 1966-06-08 | 1967-06-27 | Andonian Associates Inc | Windowless vacuum chamber for exposing a cooled device to successive samplers |
| US3398549A (en) * | 1967-02-03 | 1968-08-27 | Atomic Energy Commission Usa | Apparatus for regulating at low temperatures |
-
1974
- 1974-12-14 DE DE2459218A patent/DE2459218C3/de not_active Expired
-
1975
- 1975-03-24 SE SE7503367A patent/SE399133B/xx not_active IP Right Cessation
- 1975-03-28 JP JP50037021A patent/JPS585505B2/ja not_active Expired
- 1975-03-31 US US05/565,988 patent/US3978686A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| SE7503367L (enExample) | 1975-09-30 |
| JPS50141967A (enExample) | 1975-11-15 |
| SE399133B (sv) | 1978-01-30 |
| JPS585505B2 (ja) | 1983-01-31 |
| DE2459218A1 (de) | 1975-10-09 |
| US3978686A (en) | 1976-09-07 |
| DE2459218B2 (de) | 1977-12-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C3 | Grant after two publication steps (3rd publication) | ||
| EHJ | Ceased/non-payment of the annual fee |