CY1107737T1 - Βελτιωμενα κυστιδια εξωτερικης μεμβρανης βακτηριων - Google Patents

Βελτιωμενα κυστιδια εξωτερικης μεμβρανης βακτηριων

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Publication number
CY1107737T1
CY1107737T1 CY20071101197T CY071101197T CY1107737T1 CY 1107737 T1 CY1107737 T1 CY 1107737T1 CY 20071101197 T CY20071101197 T CY 20071101197T CY 071101197 T CY071101197 T CY 071101197T CY 1107737 T1 CY1107737 T1 CY 1107737T1
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CY
Cyprus
Prior art keywords
pellet
collecting
detergent
outer membrane
membrane vesicles
Prior art date
Application number
CY20071101197T
Other languages
English (en)
Inventor
Mariagrazia Pizza
Davide Serruto
Rini Rappuoli
Original Assignee
Novartis Vaccines And Diagnostics S.R.L.
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=9943238&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1107737(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis Vaccines And Diagnostics S.R.L. filed Critical Novartis Vaccines And Diagnostics S.R.L.
Publication of CY1107737T1 publication Critical patent/CY1107737T1/el

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/095—Neisseria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55555—Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/22—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Neisseriaceae (F)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Οι υπάρχουσες μέθοδοι της προηγούμενης τεχνικής για την παρασκευή μηνιγγιτικοκοκκικών OMV παρασκευάσματος εμπλέκουν την χρήση απορρυπαντικού κατά την διάρκεια της διάσπασης της βακτηριακής μεμβράνης. Σύμφωνα με την εφεύρεση, η διάσπαση της μεμβράνης ουσιαστικά γίνεται απουσία απορρυπαντικού. Τα OMV που προκύπτουν που διατηρούν σημαντικά ανοσογονικά συστατικά, ιδιαίτερα (i) την προστατευτική πρωτεΐνη επιφανείας NspA (ii) την πρωτεΐνη ΝΜΒ2132 και (iii) την πρωτεΐνη ΝΜΒ 1870. Η μέθοδος της εφεύρεσης τυπικά εμπλέκει τα ακόλουθα βασικά στάδια : (α) επεξεργασία βακτηριακών κυττάρων ουσιαστικά απουσία απορρυπαντικού, (β) φυγοκέντρηση της σύνθεσης του σταδίου (α) για τον διαχωρισμό των κυστιδίων της εξωτερικής μεμβράνης από τα επεξεργασμένα κύτταρα και από τα κυτταρικά κατάλοιπα και συλλογή του υπερκειμένου, (γ) εκτέλεση φυγοκέντρησης υψηλής ταχύτητας του υπερκειμένου του σταδίου (β) και συλλογή των κυστιδίων της εξωτερικής μεμβράνης σε ένα ίζημα, (δ) αναδιάλυση του ιζήματος από το στάδιο (γ) σε ένα ρυθμιστικό διάλυμα, (ε) εκτέλεση μίας δεύτερης φυγοκέντρησης υψηλής ταχύτητας σύμφωνα με το στάδιο, (γ) συλλογή κυστιδίων εξωτερικής μεμβράνης σε ένα ίζημα, (στ) αναδιάλυση του ιζήματος από το στάδιο (ε) σε ένα υδατικό διάλυμα.
CY20071101197T 2002-08-30 2007-09-17 Βελτιωμενα κυστιδια εξωτερικης μεμβρανης βακτηριων CY1107737T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0220194.5A GB0220194D0 (en) 2002-08-30 2002-08-30 Improved vesicles
EP03791150A EP1534326B1 (en) 2002-08-30 2003-09-01 Improved bacterial outer membrane vesicles

Publications (1)

Publication Number Publication Date
CY1107737T1 true CY1107737T1 (el) 2013-04-18

Family

ID=9943238

Family Applications (3)

Application Number Title Priority Date Filing Date
CY20071101197T CY1107737T1 (el) 2002-08-30 2007-09-17 Βελτιωμενα κυστιδια εξωτερικης μεμβρανης βακτηριων
CY20131100752T CY1114570T1 (el) 2002-08-30 2013-09-02 Βελτιωμενα κυστιδια βακτηριακης εξωτερικης μεμβρανης
CY20141100736T CY1115777T1 (el) 2002-08-30 2014-09-11 Βελτιωμενα κυστιδια εξωτερικης μεμβρανης βακτηριων

Family Applications After (2)

Application Number Title Priority Date Filing Date
CY20131100752T CY1114570T1 (el) 2002-08-30 2013-09-02 Βελτιωμενα κυστιδια βακτηριακης εξωτερικης μεμβρανης
CY20141100736T CY1115777T1 (el) 2002-08-30 2014-09-11 Βελτιωμενα κυστιδια εξωτερικης μεμβρανης βακτηριων

Country Status (21)

Country Link
US (2) US20060166344A1 (el)
EP (5) EP2258390A1 (el)
JP (3) JP2006503822A (el)
CN (2) CN102188700A (el)
AT (1) ATE366117T1 (el)
AU (1) AU2003263534B2 (el)
BR (1) BRPI0314094A8 (el)
CA (1) CA2497165C (el)
CY (3) CY1107737T1 (el)
DE (1) DE60314756T2 (el)
DK (3) DK1864679T3 (el)
ES (4) ES2423019T3 (el)
FR (1) FR13C0046I2 (el)
GB (1) GB0220194D0 (el)
LU (1) LU92239I2 (el)
MX (1) MXPA05002316A (el)
NZ (1) NZ538673A (el)
PT (3) PT2258389E (el)
RU (1) RU2325184C2 (el)
SI (2) SI2258389T1 (el)
WO (1) WO2004019977A2 (el)

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CA2497165C (en) 2015-10-20
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DE60314756T2 (de) 2008-03-06
BRPI0314094A8 (pt) 2018-04-03
PT2258389E (pt) 2013-08-22
CY1115777T1 (el) 2017-01-25
ES2423019T3 (es) 2013-09-17
SI1864679T1 (sl) 2014-10-30
BR0314094A (pt) 2005-11-16
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US20150258188A1 (en) 2015-09-17
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