CN1646687A - 修饰的基因沉默rna及其用途 - Google Patents

修饰的基因沉默rna及其用途 Download PDF

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Publication number
CN1646687A
CN1646687A CNA038082551A CN03808255A CN1646687A CN 1646687 A CN1646687 A CN 1646687A CN A038082551 A CNA038082551 A CN A038082551A CN 03808255 A CN03808255 A CN 03808255A CN 1646687 A CN1646687 A CN 1646687A
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viroid
nucleotide sequence
seq
chimeric
intron
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Chinese (zh)
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王明波
P·沃特豪斯
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Commonwealth Scientific and Industrial Research Organization CSIRO
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CNA038082551A 2002-03-14 2003-03-12 修饰的基因沉默rna及其用途 Pending CN1646687A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36385102P 2002-03-14 2002-03-14
US60/363,851 2002-03-14

Publications (1)

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CN1646687A true CN1646687A (zh) 2005-07-27

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US (2) US7576262B2 (https=)
EP (1) EP1487980A4 (https=)
JP (1) JP2005519607A (https=)
CN (1) CN1646687A (https=)
AU (1) AU2003209814B2 (https=)
BR (1) BR0308424A (https=)
CA (1) CA2478904A1 (https=)
IN (1) IN2004KO01268A (https=)
WO (1) WO2003076619A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104232796A (zh) * 2014-09-22 2014-12-24 北京出入境检验检疫局检验检疫技术中心 检测澳大利亚葡萄类病毒的RT-qPCR寡核苷酸及检测方法
CN118995804A (zh) * 2024-10-23 2024-11-22 青岛农业大学 一种突变型质粒及制备方法和应用
WO2025118551A1 (zh) * 2023-12-04 2025-06-12 西部(重庆)科学城种质创制大科学中心 基于CRISPR-RfxCas13d系统提高马铃薯PSTVd抗性的方法及其产品和应用

Families Citing this family (196)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999049029A1 (en) 1998-03-20 1999-09-30 Benitec Australia Ltd Control of gene expression
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
WO2000063364A2 (en) * 1999-04-21 2000-10-26 American Home Products Corporation Methods and compositions for inhibiting the function of polynucleotide sequences
US7485715B2 (en) * 1999-06-18 2009-02-03 Ceres, Inc. Sequence-determined DNA encoding AP2 domain polypeptides
US7479555B2 (en) * 1999-07-21 2009-01-20 Ceres, Inc. Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
US7612194B2 (en) 2001-07-24 2009-11-03 Monsanto Technology Llc Nucleic acid sequences from Diabrotica virgifera virgifera LeConte and uses thereof
EP2213737B1 (en) 2002-02-01 2012-11-07 Life Technologies Corporation Double-stranded oligonucleotides
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US20030166282A1 (en) 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
EP1692285B1 (en) 2003-08-15 2012-04-11 Commonwealth Scientific and Industrial Research Organisation Methods and means for altering fiber characteristics in fiber-producing plants
WO2005026356A1 (en) * 2003-09-12 2005-03-24 Commonwealth Scientific And Industrial Research Organisation Modified gene-silencing nucleic acid molecules and uses thereof
WO2005049841A1 (en) * 2003-11-17 2005-06-02 Commonwealth Scientific And Industrial Research Organisation Insect resistance using inhibition of gene expression
US20060235212A1 (en) * 2004-12-22 2006-10-19 Nickolai Alexandrov Nucleic acid sequences encoding zinc finger proteins
US8049069B2 (en) 2004-03-31 2011-11-01 Commonwealth Scientific And Industrial Research Organisation Genes involved in plant fibre development
US20060021087A1 (en) 2004-04-09 2006-01-26 Baum James A Compositions and methods for control of insect infestations in plants
EP1786917A4 (en) * 2004-07-26 2008-05-28 Enzon Pharmaceuticals Inc OPTIMIZED INTERFERON BETA-GEN
EP1778848A2 (en) 2004-08-13 2007-05-02 BASF Plant Science GmbH Compositions and methods using rna interference for control of nematodes
WO2006070227A2 (en) * 2004-10-04 2006-07-06 Devgen Nv Method for down-regulating gene expression in fungi
US20060247197A1 (en) 2004-10-04 2006-11-02 Van De Craen Marc Method for down-regulating gene expression in fungi
US7429692B2 (en) * 2004-10-14 2008-09-30 Ceres, Inc. Sucrose synthase 3 promoter from rice and uses thereof
CN101107362A (zh) 2004-10-21 2008-01-16 文甘扎公司 用于赋予对植物病害生物和植物病原体抗性的方法和材料
EP1807519B1 (en) 2004-10-29 2012-02-01 Bayer BioScience N.V. Stress tolerant cotton plants
WO2007050625A1 (en) * 2005-10-25 2007-05-03 Ceres, Inc. Modulation of triterpenoid content in plants
US9758790B2 (en) 2004-12-08 2017-09-12 Ceres, Inc. Modulating the level of components within plants
WO2007087601A2 (en) * 2006-01-25 2007-08-02 Ceres, Inc. Modulating plant protein levels
WO2008069878A2 (en) 2006-10-27 2008-06-12 Ceres, Inc. Modulating lignin in plants
US7335760B2 (en) * 2004-12-22 2008-02-26 Ceres, Inc. Nucleic acid sequences encoding zinc finger proteins
CA2598436A1 (en) * 2005-02-22 2006-08-31 Ceres, Inc. Modulating plant alkaloids
EP2338905B1 (en) 2005-02-23 2017-11-29 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US20060236421A1 (en) * 2005-04-14 2006-10-19 Pennell Roger I Secondary metabolite production via manipulation of genome methylation
US7312376B2 (en) * 2005-04-20 2007-12-25 Ceres, Inc. Regulatory regions from Papaveraceae
NZ564016A (en) 2005-05-25 2009-08-28 Pioneer Hi Bred Int Methods for improving crop plant architecture and yield by introducing recombinant expression cassette
US8124839B2 (en) * 2005-06-08 2012-02-28 Ceres, Inc. Identification of terpenoid-biosynthesis related regulatory protein-regulatory region associations
AU2006292362B2 (en) 2005-09-16 2013-02-14 Monsanto Technology Llc Methods for genetic control of insect infestations in plants and compositions thereof
WO2007041536A2 (en) * 2005-09-30 2007-04-12 Ceres, Inc. Modulating plant tocopherol levels
EP2112223A3 (en) 2005-11-10 2010-01-27 Pioneer Hi-Bred International Inc. DOF (DNA binding with one finger) sequences and method of use
US8222482B2 (en) * 2006-01-26 2012-07-17 Ceres, Inc. Modulating plant oil levels
WO2007092704A2 (en) 2006-02-09 2007-08-16 Pioneer Hi-Bred International, Inc. Genes for enhancing nitrogen utilization efficiency in crop plants
US20070199090A1 (en) * 2006-02-22 2007-08-23 Nestor Apuya Modulating alkaloid biosynthesis
WO2007120989A2 (en) * 2006-02-24 2007-10-25 Ceres, Inc. Shade regulatory regions
US20090222957A1 (en) * 2006-04-07 2009-09-03 Ceres Inc. Regulatory protein-regulatory region associations related to alkaloid biosynthesis
EP2251349A1 (en) 2006-04-19 2010-11-17 Pioneer Hi-Bred International, Inc. Isolated polynucleotide molecules corresponding to mutant and wild-type alleles of the maize D9 gene and methods of use
WO2007133804A2 (en) * 2006-05-15 2007-11-22 Ceres, Inc. Modulation of oil levels in plants
WO2007147068A2 (en) * 2006-06-14 2007-12-21 Ceres, Inc. Increasing uv-b tolerance in plants
US20130191941A1 (en) 2006-07-05 2013-07-25 Shing Kwok Modulating light response pathways in plants, increasing light-related tolerances in plants, and increasing biomass in plants
WO2008064128A2 (en) 2006-11-22 2008-05-29 Ceres, Inc. Broadly expressing regulatory regions
US20100151109A1 (en) * 2006-12-15 2010-06-17 Amr Saad Ragab Modulation of plant protein levels
US9057073B2 (en) 2007-01-11 2015-06-16 Bayer Cropscience N.V. Differential expression of subgenome specific alleles in cotton and uses thereof
MX2009007608A (es) 2007-02-06 2009-07-27 Basf Plant Science Gmbh Composiciones y metodos que utilizan arn de interferencia para el control de nematodos.
PL2129680T3 (pl) 2007-03-21 2015-10-30 Brookhaven Science Ass Llc Łączone kompozycje cząsteczek antysensownych o strukturze spinki do włosów oraz sposoby modulacji ekspresji
US20100154076A1 (en) 2007-05-25 2010-06-17 Cropdesign N.V. Yield Enhancement In Plants By Modulation of Maize Alfins
ES2651911T3 (es) 2007-08-14 2018-01-30 Commonwealth Scientific And Industrial Research Organisation Métodos mejorados de silenciamiento génico
US8097712B2 (en) * 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
EP2215238A2 (en) 2007-11-20 2010-08-11 Pioneer Hi-Bred International Inc. Maize ethylene signaling genes and modulation of same for improved stress tolerance in plants
AU2009221899B2 (en) 2008-03-04 2015-09-03 Washington State University Compositions and methods for differential regulation of fatty acid unsaturation in membrane lipids and seed oil
MX2010012883A (es) 2008-05-26 2010-12-14 Bayer Bioscience Nv Metodos y medios para modificar la resistencia de las fibras en plantas que producen fibras.
EP2315519B1 (en) 2008-07-21 2016-08-17 Commonwealth Scientific and Industrial Research Organisation Improved cottonseed oil and uses
US8419145B2 (en) * 2008-07-25 2013-04-16 Eastman Kodak Company Inkjet printhead and method of printing with multiple drop volumes
US8298794B2 (en) * 2008-10-09 2012-10-30 Ceres, Inc. Cinnamyl-alcohol dehydrogenases
CN102197137A (zh) 2008-10-30 2011-09-21 先锋国际良种公司 对谷氨酰胺合成酶(gs)进行操作以改善高等植物中的氮利用效率和籽粒产量
CA2743707A1 (en) 2008-12-04 2010-06-10 Pioneer Hi-Bred International, Inc. Methods and compositions for enhanced yield by targeted expression of knotted1
US8309530B2 (en) 2009-02-04 2012-11-13 Washington State University Compositions and methods for modulating ghrelin-mediated conditions
WO2010101818A1 (en) 2009-03-02 2010-09-10 Pioneer Hi-Bred International, Inc. Nac transcriptional activators involved in abiotic stress tolerance
CA2754956A1 (en) 2009-03-20 2010-09-23 Basf Plant Science Company Gmbh Nematode-resistant transgenic plants
US20100257634A1 (en) * 2009-04-03 2010-10-07 Venganza Inc. Bioassay for gene silencing constructs
EA201171245A1 (ru) 2009-04-14 2012-05-30 Пайонир Хай-Бред Интернешнл, Инк. Модуляция acc-синтазы, улучшающая урожайность растений при условиях низкого содержания азота
MX2011011678A (es) 2009-05-04 2011-12-06 Pioneer Hi Bred Int Mejora de la produccion en plantas mediante la modulacion del factor de transcripcion ap2.
EP2427180B1 (en) 2009-05-05 2016-04-13 Beeologics Inc. Prevention and treatment of nosema disease in bees
CA2735978A1 (en) * 2009-06-02 2010-12-09 Incorporated Administrative Agency National Agriculture And Food Researc H Organization Method for simultaneous detection of viroids, pstvd and tcdvd
CA2765287C (en) 2009-06-15 2018-12-11 Bayer Bioscience N.V. Nicotiana benthamiana plants deficient in xylosyltransferase activity
CA3208000A1 (en) 2009-07-20 2011-01-27 Ceres, Inc. Transgenic plants having increased biomass
WO2011011273A1 (en) 2009-07-24 2011-01-27 Pioneer Hi-Bred International, Inc. The use of dimerization domain component stacks to modulate plant architecture
CN102597229A (zh) 2009-10-02 2012-07-18 先锋国际良种公司 下调acc合酶以改进植物性能
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
US8927812B2 (en) 2009-12-09 2015-01-06 Basf Plant Science Company Gmbh Methods for increasing the resistance of plants to fungi by silencing the fungal SMT1-gene
CN102906267A (zh) 2010-01-06 2013-01-30 先锋国际良种公司 玉米光合作用组织和非光合作用组织昼夜节律的鉴定及其在改良作物方面的用途
EP2539362A1 (en) 2010-02-23 2013-01-02 BASF Plant Science Company GmbH Nematode-resistant transgenic plants
ES2641642T3 (es) 2010-03-08 2017-11-10 Monsanto Technology Llc Moléculas de polinucleótido para regulación génica en plantas
CA3130705A1 (en) 2010-04-15 2011-10-20 National Research Council Of Canada Genes and proteins for aromatic polyketide synthesis
AR080745A1 (es) 2010-05-06 2012-05-02 Du Pont Gen y proteina acc (acido 1-aminociclopropano-1-carboxilico) sintasa 3 de maiz y sus usos
WO2011140329A1 (en) 2010-05-06 2011-11-10 Ceres, Inc. Transgenic plants having increased biomass
EP2590996B1 (en) 2010-07-08 2016-05-25 Bayer CropScience NV Glucosinolate transporter protein and uses thereof
EP2593468B1 (en) 2010-07-12 2020-06-10 The State of Israel, Ministry of Agriculture and Rural Development, Agricultural Research Organization, (A.R.O.), Volcani Center Isolated polynucleotides and methods and plants using same for regulating plant acidity
US8822427B2 (en) 2010-10-27 2014-09-02 Harrisvaccines Methods and compositions to protect aquatic invertebrates from disease
WO2012058223A1 (en) 2010-10-27 2012-05-03 Ceres, Inc. Transgenic plants having altered biomass composition
US10004797B2 (en) 2010-10-27 2018-06-26 Harrisvaccines, Inc. Method of rapidly producing improved vaccines for animals
EP2633049B1 (en) 2010-10-27 2018-10-10 Harrisvaccines Inc Methods and compositions to protect aquatic invertebrates from disease
EP2655629B1 (en) 2010-12-24 2024-04-24 BASF Agricultural Solutions Seed US LLC Brassica plant comprising a mutant alcatraz allele
WO2012101118A1 (en) 2011-01-24 2012-08-02 Bayer Cropscience N.V. Use of the rd29 promoter or fragments thereof for stress-inducible expression of transgenes in cotton
MX2013010911A (es) 2011-03-23 2015-03-03 Pioneer Hi Bred Int Metodos para producir un locus de rasgo transgenico complejo.
US20120266324A1 (en) 2011-04-15 2012-10-18 Pioneer Hi Bred International Inc. Self-Reproducing Hybrid Plants
CA2833876A1 (en) 2011-04-29 2012-11-01 Pioneer Hi-Bred International, Inc. Down-regulation of a homeodomain-leucine zipper i-class homeobox gene for improved plant performance
BR112013031723B1 (pt) 2011-06-10 2020-10-13 Temasek Life Sciences Laboratory Limited construção de polinucleotídeo, método para transformação de uma célula fúngica e promotor isolado
US20140216118A1 (en) 2011-06-14 2014-08-07 Synthon Biopharmaceuticals B.V. Compositions and Methods for Making and Biocontaining Auxotrophic Transgenic Plants
BR112013033176A2 (pt) 2011-06-21 2018-06-12 E I Du Pont De Nemouras And Company método para produzir uma modificação direcionada em um gene de fertilidade masculina, planta, molécula de ácido nucleio isolada, construto de expressão
EP2551348B1 (en) 2011-07-29 2014-09-24 Icon Genetics GmbH Production of galactosylated N-glycans in plants
WO2013023992A1 (en) 2011-08-12 2013-02-21 Bayer Cropscience Nv Guard cell-specific expression of transgenes in cotton
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
MX361938B (es) 2011-09-13 2018-12-19 Monsanto Technology Llc Métodos y composiciones para el control de malezas.
EP3382027A3 (en) 2011-09-13 2018-10-31 Monsanto Technology LLC Methods and compositions for weed control
CA2848695A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and composition for weed control comprising inhibiting ppg oxidase
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
WO2013040057A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
US10196664B2 (en) 2011-10-04 2019-02-05 Icon Genetics Gmbh Nicotiana benthamiana plants deficient in fucosyltransferase activity
US20140251317A1 (en) 2011-10-12 2014-09-11 Bayer Cropscience Ag Plants with decreased activity of a starch dephosphorylating enzyme
BR112014008723A2 (pt) 2011-10-12 2019-09-24 Bayer Cropscience Ag plantas com diminuição da atividade de uma enzima de desforilação de àmido
MX2014005212A (es) 2011-10-31 2014-11-25 Pioneer Hi Bred Int Mejoramiento de la tolerancia a la sequia, eficiencia de uso de nitrogeno y rendimiento de una planta.
BR122020026845B1 (pt) 2011-11-02 2021-04-13 Ceres, Inc Método de aumento do rendimento de planta em solo contendo níveis elevados de al3+, método de aumento da tolerância de uma planta
BR112014013853A2 (pt) 2011-12-09 2020-01-14 Ceres, Inc. uso de um ácido nucleico isolado, método de obtenção de uma célula vegetal transgênica, método de obtenção de uma planta transgênica, método de produção de uma planta, método de produção de biomassa, método de processamento de biomassa, método de alteração da composição de biomassa, método de modulação de composição de biomassa, método de produção de um produto de forragem
UA116097C2 (uk) 2011-12-11 2018-02-12 Зе Стейт Оф Ізраел, Міністрі Оф Агрікалче Енд Руерал Девелопмент, Агрікалчерал Рісьоч Організейшн, (А.Р.О.), Волкані Сентре Спосіб модуляції провідності устячка рослини
WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
EA201491670A1 (ru) 2012-03-13 2015-07-30 Пайонир Хай-Бред Интернэшнл, Инк. Генетическое снижение мужской репродуктивной функции у растений
IN2014KN02312A (https=) 2012-04-19 2015-05-01 Temasek Life Sciences Lab Ltd
MX360866B (es) 2012-05-24 2018-11-09 A B Seeds Ltd Composiciones y métodos para silenciar la expresión genética.
US9968041B2 (en) 2012-07-06 2018-05-15 Bayer Cropscience Nv Soybean ROD1 gene sequences and uses thereof
EA201590164A1 (ru) 2012-07-06 2015-05-29 Байер Кропсайенс Нв Растения brassica с модифицированным составом масла семян
CA2878286C (en) 2012-07-06 2023-06-13 Bayer Cropscience Nv Brassica rod1 gene sequences and uses thereof
US10006041B2 (en) 2012-08-16 2018-06-26 Vib Vzw Means and methods for altering the lignin pathway in plants
CA2888264A1 (en) 2012-10-18 2014-04-24 Monsanto Technology Llc Methods and compositions for plant pest control
UY35252A (es) 2013-01-01 2014-07-31 Seeds Ltd Ab MÉTODOS PARA INTRODUCIR dsRNA EN SEMILLAS DE PLANTAS PARA MODULAR LA EXPRESIÓN GENÉTICA
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
US20150376637A1 (en) 2013-01-29 2015-12-31 The University Court Of The University Of Glasgow Methods and means for increasing stress tolerance and biomass in plants
WO2014164014A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Genes for improving nutrient uptake and abiotic stress tolerance in plants
BR112015023051A2 (pt) 2013-03-13 2017-11-14 Monsanto Technology Llc método para controle de ervas daninhas, composição herbicida, cassete de expressão microbiano e método de produção de polinucleotídeo
US20160017360A1 (en) 2013-03-13 2016-01-21 Pioneer Hi-Bred International, Inc. Functional expression of bacterial major facilitator superfamily mfs gene in maize to improve agronomic traits and grain yield
US10612019B2 (en) 2013-03-13 2020-04-07 Monsanto Technology Llc Methods and compositions for weed control
US20160010101A1 (en) 2013-03-13 2016-01-14 Pioneer Hi-Bred International, Inc. Enhanced nitrate uptake and nitrate translocation by over- expressing maize functional low-affinity nitrate transporters in transgenic maize
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
BR112015023304A2 (pt) 2013-03-14 2017-07-18 Pioneer Hi Bred Int método para melhorar a tolerância ao estresse abiótico de uma planta, planta e semente
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
CA2903555A1 (en) 2013-03-15 2014-09-18 Pioneer Hi-Bred International, Inc. Compositions and methods of use of acc oxidase polynucleotides and polypeptides
US10017778B2 (en) 2013-03-21 2018-07-10 Vib Vzw Means and methods for the reduction of photorespiration in crops
EP3007547B1 (en) 2013-06-11 2018-07-18 Syngenta Participations AG Methods for generating transgenic plants
CA3177649A1 (en) 2013-07-01 2015-01-08 Basf Se Methods and means for modulating flowering time in monocot plants
EP3030663B1 (en) 2013-07-19 2019-09-04 Monsanto Technology LLC Compositions and methods for controlling leptinotarsa
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
EA036403B1 (ru) 2013-09-24 2020-11-06 Басф Се Белок с активностью целлюлоза:ксилоглюкан-эндотрансглюкозилазы (cxe) и его применение
CA2928830A1 (en) 2013-10-29 2015-05-07 Shai J. Lawit Self-reproducing hybrid plants
AU2014341879B2 (en) 2013-11-04 2020-07-23 Greenlight Biosciences, Inc. Compositions and methods for controlling arthropod parasite and pest infestations
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
WO2015108982A2 (en) 2014-01-15 2015-07-23 Monsanto Technology Llc Methods and compositions for weed control using epsps polynucleotides
WO2015116680A1 (en) 2014-01-30 2015-08-06 Two Blades Foundation Plants with enhanced resistance to phytophthora
US11525143B2 (en) 2014-02-28 2022-12-13 Universidade Federal Do Rio De Janeiro Method for promoting an increase in plant biomass, productivity, and drought resistance
BR102014004881A2 (pt) 2014-02-28 2016-02-02 Univ Rio De Janeiro método para promover um aumento da biomassa e produtividade vegetal e da tolerância à seca
WO2015153339A2 (en) 2014-04-01 2015-10-08 Monsanto Technology Llc Compositions and methods for controlling insect pests
US9101100B1 (en) 2014-04-30 2015-08-11 Ceres, Inc. Methods and materials for high throughput testing of transgene combinations
WO2015171603A1 (en) 2014-05-06 2015-11-12 Two Blades Foundation Methods for producing plants with enhanced resistance to oomycete pathogens
CN106795515B (zh) 2014-06-23 2021-06-08 孟山都技术公司 用于经由rna干扰调控基因表达的组合物和方法
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
CN107075523B (zh) 2014-06-27 2022-04-19 加拿大国家研究委员会 来自大麻的大麻环萜酚酸合酶
WO2016005449A1 (en) 2014-07-08 2016-01-14 Vib Vzw Means and methods to increase plant yield
WO2016007640A1 (en) 2014-07-10 2016-01-14 Benson Hill Biosystems, Inc. Compositions and methods for increasing plant growth and yield
WO2016011179A2 (en) 2014-07-15 2016-01-21 Ceres, Inc. Methods of increasing crop yield under abiotic stress
CN106604993A (zh) 2014-07-29 2017-04-26 孟山都技术公司 用于控制昆虫害虫的组合物和方法
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
WO2016064347A1 (en) 2014-10-22 2016-04-28 Temasek Life Sciences Laboratory Limited Terpene synthases from ylang ylang (cananga odorata var. fruticosa)
WO2016113333A1 (en) 2015-01-16 2016-07-21 Bayer Cropscience Nv Leaf-preferential promoters and uses thereof
CN108064288B (zh) 2015-01-22 2021-11-26 孟山都技术公司 用于控制叶甲属的组合物和方法
WO2016128519A1 (en) 2015-02-12 2016-08-18 Bayer Cropscience Nv Shoot apex-preferential promoters and uses thereof
EP3302053B1 (en) 2015-06-02 2021-03-17 Monsanto Technology LLC Compositions and methods for delivery of a polynucleotide into a plant
WO2016196782A1 (en) 2015-06-03 2016-12-08 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
BR112018002567B1 (pt) 2015-08-07 2023-10-31 BASF Agricultural Solutions Seed US LLC Gene recombinante, célula hospedeira, método de produção de uma planta transgênica, método de efetuação de expressão preferencial em raiz de um ácido nucleico, método para alterar a tolerância ao estresse biótico ou abiótico, arquitetura de raiz, eficiência no uso de nutriente, ou rendimento de uma planta e uso de um ácido nucleico isolado
WO2017062790A1 (en) 2015-10-09 2017-04-13 Two Blades Foundation Cold shock protein receptors and methods of use
AU2016336812A1 (en) 2015-10-16 2018-06-07 Bayer Cropscience Nv Brassica plants with altered properties in seed production
WO2017070458A2 (en) 2015-10-23 2017-04-27 Donald Danforth Plant Science Center Genes encoding c4 transporter proteins for use in increasing crop plant yield
CN105463011B (zh) * 2015-12-22 2019-02-01 华南农业大学 一种植物生长负调控顺式元件se1及其在调控植物株高中的应用
AU2017234920B2 (en) 2016-03-18 2023-08-17 E. I. Du Pont De Nemours And Company Methods and compositions for producing clonal, non-reduced, non-recombined gametes
JP2019509053A (ja) 2016-03-24 2019-04-04 ウニフェルシタイト・ライデン 植物にトランスフェクトする方法及びランダム組込み事象の低減方法
US10975380B2 (en) 2016-04-11 2021-04-13 Basf Agricultural Solutions Seed, Us Llc Seed-specific and endosperm-preferental promoters and uses thereof
US20190127747A1 (en) 2016-04-11 2019-05-02 Bayer Cropscience Nv Seed-specific and endosperm-preferential promoters and uses thereof
US20210214737A1 (en) 2016-04-13 2021-07-15 Bayer Cropscience Nv Seed-specific and embryo-preferential promoters and uses thereof
US10900046B2 (en) 2016-04-13 2021-01-26 BASF Agricultural Solutions Seed US LLC Seed- and funiculus-preferential promoters and uses thereof
AU2018239406A1 (en) 2017-03-23 2019-09-26 Basf Se Anther-specific promoter and uses thereof
BR112019024630A2 (pt) 2017-05-24 2020-06-23 BASF Agricultural Solutions Seed US LLC Ácido nucleico isolado, gene recombinante, célula hospedeira, planta, partes e sementes de plantas, planta ou célula vegetal ou parte de planta ou semente, métodos para produzir uma planta transgênica e para efetuar a expressão induzível e uso do ácido nucleico
IL273245B2 (en) 2017-09-15 2025-03-01 Commw Scient Ind Res Org RNA molecules
BR112020008092A2 (pt) 2017-10-24 2020-09-15 BASF Agricultural Solutions Seed US LLC método para conferir tolerância a um herbicida e planta de soja transgênica
WO2019083808A1 (en) 2017-10-24 2019-05-02 Basf Se IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS
EP3502259A1 (en) 2017-12-19 2019-06-26 Universiteit Leiden A combinational strategy for reducing random integration events when transfecting plants
AU2019296278A1 (en) 2018-06-27 2021-02-04 Basf Se Thermostable rubisco activase and uses thereof
US11473086B2 (en) 2019-06-19 2022-10-18 Ut-Battelle, Llc Loss of function alleles of PtEPSP-TF and its regulatory targets in rice
WO2021004838A2 (en) 2019-07-05 2021-01-14 BASF Agricultural Solutions Seed US LLC Rubisco activase with reduced adp inhibition and uses thereof
US20220298522A1 (en) 2019-07-30 2022-09-22 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization Methods of controlling cannabinoid synthesis in plants or cells and plants and cells produced thereby
WO2021243271A2 (en) * 2020-05-29 2021-12-02 Front Range Biosciences, Inc. Methods and compositions for pathogen detection in plants
MX2023000694A (es) * 2020-07-20 2023-04-18 Flagship Pioneering Inc Polinucleótidos obtenidos a partir de viroides para la modificación de plantas.
US20250066865A1 (en) * 2021-02-19 2025-02-27 Purple City Labs, Inc. Detection of pathogens in plants
CN118019447A (zh) 2021-08-06 2024-05-10 Kws蔬菜有限责任公司 菠菜对霜霉病的持久抗性
AU2022358470A1 (en) 2021-10-01 2024-04-11 Basf Se Plants with improved properties
EP4408860A1 (en) 2021-10-01 2024-08-07 Basf Se Wheat plants with an increased yield
WO2024099765A2 (en) 2022-11-10 2024-05-16 BASF Agricultural Solutions Seed US LLC Transcription regulating nucleotide sequences and methods of use
EP4477069A1 (en) 2023-06-16 2024-12-18 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Unreduced clonal gamete formation and polyploid genome design in the solanaceae
NL2035852B1 (en) 2023-09-21 2025-03-28 Academisch Ziekenhuis Leiden Enhancement of t cell mediated therapies

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
DE3486053T3 (de) 1983-10-20 2004-01-08 The Research Foundation Of State University Of New York Regulierung von Gen-Expression durch Translationshemmung unter Verwendung von m-RNS hinderndem Komplementär-RNS.
US6617496B1 (en) 1985-10-16 2003-09-09 Monsanto Company Effecting virus resistance in plants through the use of negative strand RNAs
IL81737A (en) 1986-03-28 1992-11-15 Calgene Inc Regulation of gene expression in plant cells
US5037745A (en) 1986-04-01 1991-08-06 University Of Medicine And Dentistry Of New Jersey Plasmid for the overproduction of bacteriophage T3 RNA polymerase, transcription vectors that carry a promoter recognized by its polymerase, gene coding for T3 RNA polymerase and application of these plasmids
JP3026985B2 (ja) 1987-10-20 2000-03-27 プラント・ジェネティック・システムズ・エヌ・ブイ 遺伝子導入植物内の修飾された貯蔵種子タンパク質遺伝子の発現による生物学的に活性なペプチドの製造方法
GB8810120D0 (en) 1988-04-28 1988-06-02 Plant Genetic Systems Nv Transgenic nuclear male sterile plants
CA2103573C (en) 1991-02-08 2005-04-26 Frank Michiels Stamen-specific promoters from rice
US5908779A (en) 1993-12-01 1999-06-01 University Of Connecticut Targeted RNA degradation using nuclear antisense RNA
US6211431B1 (en) 1994-08-30 2001-04-03 Commonwealth Scientific And Industrial Research Organization Plant transcription regulators from circovirus
WO1997013865A1 (en) 1995-10-06 1997-04-17 Plant Genetic Systems, N.V. Seed shattering
DE19631919C2 (de) 1996-08-07 1998-07-16 Deutsches Krebsforsch Anti-Sinn-RNA mit Sekundärstruktur
AU3369900A (en) * 1999-02-19 2000-09-04 General Hospital Corporation, The Gene silencing
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
ATE507300T1 (de) 2000-06-23 2011-05-15 Du Pont Rekombinante konstrukte und ihre verwendung bei der reduzierung der genexpression
AU2001291656A1 (en) 2000-06-30 2002-01-08 Willem Broekaert Gene silencing vector
WO2002010365A2 (en) 2000-08-02 2002-02-07 The Board Of Regents Of The University Of Nebraska Down-regulation of single genes and simultaneous down-regulation of multiple genes by nuclear localization of rna transcripts
NZ526507A (en) * 2001-01-26 2005-07-29 Commw Scient Ind Res Org Methods and means for producing efficient silencing construct using recombinational cloning

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104232796A (zh) * 2014-09-22 2014-12-24 北京出入境检验检疫局检验检疫技术中心 检测澳大利亚葡萄类病毒的RT-qPCR寡核苷酸及检测方法
WO2025118551A1 (zh) * 2023-12-04 2025-06-12 西部(重庆)科学城种质创制大科学中心 基于CRISPR-RfxCas13d系统提高马铃薯PSTVd抗性的方法及其产品和应用
CN118995804A (zh) * 2024-10-23 2024-11-22 青岛农业大学 一种突变型质粒及制备方法和应用

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