EA201590164A1 - Растения brassica с модифицированным составом масла семян - Google Patents

Растения brassica с модифицированным составом масла семян

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Publication number
EA201590164A1
EA201590164A1 EA201590164A EA201590164A EA201590164A1 EA 201590164 A1 EA201590164 A1 EA 201590164A1 EA 201590164 A EA201590164 A EA 201590164A EA 201590164 A EA201590164 A EA 201590164A EA 201590164 A1 EA201590164 A1 EA 201590164A1
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EA
Eurasian Patent Office
Prior art keywords
plants
oil composition
seeds oil
modified seeds
brassica
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Application number
EA201590164A
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English (en)
Inventor
Петер Денольф
Шуаньгуй Бай
Джон Брауз
Original Assignee
Байер Кропсайенс Нв
Вашингтон Стейт Юниверсити
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Application filed by Байер Кропсайенс Нв, Вашингтон Стейт Юниверсити filed Critical Байер Кропсайенс Нв
Publication of EA201590164A1 publication Critical patent/EA201590164A1/ru

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/20Brassicaceae, e.g. canola, broccoli or rucola
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • C11B1/10Production of fats or fatty oils from raw materials by extracting
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11CFATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C3/00Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
    • C11C3/003Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fatty acids with alcohols
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288Transferases for other substituted phosphate groups (2.7.8)
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
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    • C12Y207/08002Diacylglycerol cholinephosphotransferase (2.7.8.2)
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    • C12Q2600/00Oligonucleotides characterized by their use
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  • Developmental Biology & Embryology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Animal Husbandry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к растениям Brassica, несущим мутантные гены ROD1, последовательностям нуклеиновых кислот ROD1 и белкам ROD1, а также к способам получения и идентификации указанных растений и аллелей, которые можно использовать в растениях с повышенными уровнями С18:1 в семенах.
EA201590164A 2012-07-06 2013-07-04 Растения brassica с модифицированным составом масла семян EA201590164A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261668529P 2012-07-06 2012-07-06
PCT/EP2013/064190 WO2014006162A1 (en) 2012-07-06 2013-07-04 Brassica plants with modified seed oil composition

Publications (1)

Publication Number Publication Date
EA201590164A1 true EA201590164A1 (ru) 2015-05-29

Family

ID=48794062

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201590164A EA201590164A1 (ru) 2012-07-06 2013-07-04 Растения brassica с модифицированным составом масла семян

Country Status (10)

Country Link
US (1) US10119147B2 (ru)
EP (1) EP2877583A1 (ru)
JP (1) JP2015524251A (ru)
CN (1) CN104603276A (ru)
AR (1) AR091671A1 (ru)
AU (1) AU2013285460A1 (ru)
CA (1) CA2878289A1 (ru)
CL (1) CL2015000016A1 (ru)
EA (1) EA201590164A1 (ru)
WO (1) WO2014006162A1 (ru)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201590164A1 (ru) 2012-07-06 2015-05-29 Байер Кропсайенс Нв Растения brassica с модифицированным составом масла семян
WO2019233863A1 (de) 2018-06-04 2019-12-12 Bayer Aktiengesellschaft Herbizid wirksame bizyklische benzoylpyrazole
WO2020049157A1 (en) * 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
CN112969792A (zh) * 2018-09-07 2021-06-15 巴斯夫植物科学有限公司 用于在植物中产生高水平pufa的改进方法
US20210317467A1 (en) * 2018-09-07 2021-10-14 Basf Plant Science Copmany Gmbh Improved method for the production of high levels of pufa in plants

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2018274T5 (es) 1986-03-11 1996-12-16 Plant Genetic Systems Nv Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica.
AU2811889A (en) 1987-10-20 1989-05-23 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
GB8810120D0 (en) 1988-04-28 1988-06-02 Plant Genetic Systems Nv Transgenic nuclear male sterile plants
WO1992013956A1 (en) 1991-02-08 1992-08-20 Plant Genetic Systems, N.V. Stamen-specific promoters from rice
FR2673643B1 (fr) 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
FR2673642B1 (fr) 1991-03-05 1994-08-12 Rhone Poulenc Agrochimie Gene chimere comprenant un promoteur capable de conferer a une plante une tolerance accrue au glyphosate.
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
WO1993006239A1 (en) 1991-09-24 1993-04-01 Keygene N.V. Selective restriction fragment amplification: a general method for dna fingerprinting
ATE266734T1 (de) 1994-08-30 2004-05-15 Commw Scient Ind Res Org Pflanzentraskriptionsregulator von circovirus
EP0853676B1 (en) 1995-10-06 2006-06-21 Bayer BioScience N.V. Seed shattering
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US5977436A (en) 1997-04-09 1999-11-02 Rhone Poulenc Agrochimie Oleosin 5' regulatory region for the modification of plant seed lipid composition
ATE507299T1 (de) 1998-04-08 2011-05-15 Commw Scient Ind Res Org Verfahren und mittel zum erhalt von veränderten phänotypen
US6405019B1 (en) 1998-06-30 2002-06-11 Ericsson, Inc. Method and apparatus for controlling a performance characteristic of an electronic device
US7495148B2 (en) 2000-07-14 2009-02-24 Unicrop Ltd Double recoverable block of function
US7081565B2 (en) 2000-12-01 2006-07-25 Board Of Trustees Operating Michigan State University Plant seed specific promoters
NZ526507A (en) 2001-01-26 2005-07-29 Commw Scient Ind Res Org Methods and means for producing efficient silencing construct using recombinational cloning
WO2003076619A1 (en) 2002-03-14 2003-09-18 Commonwealth Scientific And Industrial Research Organisation Modified gene-silencing rna and uses thereof
DE10211617A1 (de) 2002-03-15 2003-09-25 Inst Pflanzengenetik & Kultur Neuer Promotor mit erhöhtem Expressionsprofil
US7423198B2 (en) 2002-05-15 2008-09-09 Viterra, Inc. High oleic acid Brassica juncea
US8304610B2 (en) * 2002-05-15 2012-11-06 Viterra, Inc. Brassica juncea lines with high oleic acid profile in seed oil
AU2006203892B2 (en) 2005-01-07 2011-03-24 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Method to trigger RNA interference
CN101155921B (zh) 2005-04-04 2013-02-06 拜尔作物科学公司 用于去除所选择dna序列的方法和手段
WO2009002150A1 (en) 2007-06-22 2008-12-31 Keygene N.V. Targeted nucleotide exchange with improved modified oligonucleotides
CN101688220B (zh) 2007-07-09 2017-04-05 拜尔作物科学公司 包含突变酰基‑acp硫酯酶等位基因的芸苔属植物
US7814794B2 (en) 2007-09-07 2010-10-19 Pixart Imaging Inc. Micromachined sensors
AU2008321081B2 (en) * 2007-11-13 2014-06-19 Pioneer Hi-Bred International, Inc. Brassica juncea lines with a canola fatty acid profile
CN103397021A (zh) 2007-12-04 2013-11-20 陶氏环球技术有限责任公司 来自蓖麻植物的种子优选性基因启动子
AU2008337600A1 (en) 2007-12-14 2009-06-25 Basf Plant Science Gmbh Promoters from brassica napus for seed specific gene expression
JP5822466B2 (ja) * 2008-03-04 2015-11-24 ワシントン ステイト ユニバーシティー 種子油および膜脂質における脂肪酸不飽和化の示差的調節のための組成物および方法
JP5158639B2 (ja) 2008-04-11 2013-03-06 独立行政法人農業生物資源研究所 植物の内胚乳に特異的に発現する遺伝子および該遺伝子のプロモーター、並びにそれらの利用
CN102712911B (zh) * 2009-11-20 2016-01-13 拜尔作物科学公司 包含突变fad3等位基因的芸苔属植物
EA201590164A1 (ru) 2012-07-06 2015-05-29 Байер Кропсайенс Нв Растения brassica с модифицированным составом масла семян

Also Published As

Publication number Publication date
CA2878289A1 (en) 2014-01-09
JP2015524251A (ja) 2015-08-24
CN104603276A (zh) 2015-05-06
AR091671A1 (es) 2015-02-18
CL2015000016A1 (es) 2015-06-26
US20150159164A1 (en) 2015-06-11
US10119147B2 (en) 2018-11-06
EP2877583A1 (en) 2015-06-03
WO2014006162A1 (en) 2014-01-09
AU2013285460A1 (en) 2015-01-22

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