CN1304596C - 检测系统 - Google Patents

检测系统 Download PDF

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Publication number
CN1304596C
CN1304596C CNB028141334A CN02814133A CN1304596C CN 1304596 C CN1304596 C CN 1304596C CN B028141334 A CNB028141334 A CN B028141334A CN 02814133 A CN02814133 A CN 02814133A CN 1304596 C CN1304596 C CN 1304596C
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China
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label
probe
amplification
nucleic acid
sequence
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Expired - Fee Related
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CNB028141334A
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English (en)
Chinese (zh)
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CN1527885A (zh
Inventor
M·A·李
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UK Secretary of State for Defence
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UK Secretary of State for Defence
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Publication of CN1527885A publication Critical patent/CN1527885A/zh
Application granted granted Critical
Publication of CN1304596C publication Critical patent/CN1304596C/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Eye Examination Apparatus (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Geophysics And Detection Of Objects (AREA)
CNB028141334A 2001-05-25 2002-05-24 检测系统 Expired - Fee Related CN1304596C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0112868.5 2001-05-25
GBGB0112868.5A GB0112868D0 (en) 2001-05-25 2001-05-25 Detection system

Publications (2)

Publication Number Publication Date
CN1527885A CN1527885A (zh) 2004-09-08
CN1304596C true CN1304596C (zh) 2007-03-14

Family

ID=9915364

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB028141334A Expired - Fee Related CN1304596C (zh) 2001-05-25 2002-05-24 检测系统

Country Status (14)

Country Link
US (2) US7700275B2 (https=)
EP (1) EP1390542B1 (https=)
JP (1) JP2004532044A (https=)
KR (1) KR20040024556A (https=)
CN (1) CN1304596C (https=)
AT (1) ATE298804T1 (https=)
AU (1) AU2002311414B2 (https=)
CA (1) CA2448211C (https=)
DE (1) DE60204874T2 (https=)
DK (1) DK1390542T3 (https=)
ES (1) ES2240753T3 (https=)
GB (1) GB0112868D0 (https=)
PT (1) PT1390542E (https=)
WO (1) WO2002097132A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108351349A (zh) * 2015-09-10 2018-07-31 因斯利克萨公司 用于多路定量核酸扩增的方法和系统

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
GB0223563D0 (en) * 2002-10-10 2002-11-20 Secr Defence Detection system
RU2460804C2 (ru) * 2004-10-18 2012-09-10 Брандейс Юнивесити Способ гомогенной детекции по меньшей мере одного продукта одноцепочечной амплификации
WO2006121277A1 (en) * 2005-05-09 2006-11-16 Korea Research Institute Of Standards And Science Fret probes for fluorescent detection of the epsps gene
KR100764559B1 (ko) * 2005-05-09 2007-10-08 한국표준과학연구원 Ｅｐｓｐｓ 유전자 검색에 활용할 수 있는 ｆｒｅｔ형광검출용 프로브
CN100420943C (zh) * 2005-06-23 2008-09-24 李卫 Dna分子内杂交-聚丙烯酰胺凝胶电泳方法
US8968700B2 (en) * 2005-08-11 2015-03-03 The Board Of Trustees Of The Leland Stanford Junior University Imaging of protease activity in live cells using activity based probes
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
CN100526461C (zh) * 2006-07-10 2009-08-12 中国农业科学院棉花研究所 获得植物内含子序列扩增多态性的方法及其专用引物
JP2009044967A (ja) * 2007-08-14 2009-03-05 Sony Corp 二本鎖核酸の形成に係る情報を得る方法
US8658366B2 (en) * 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
GB201007868D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Sequence detection assay
WO2012118715A2 (en) 2011-02-28 2012-09-07 The Board Of Trustees Of Leland Stanford Junior University Non-peptidic quenched fluorescent imaging probes
DE102011117520A1 (de) * 2011-11-03 2013-05-08 Frank Schleifenbaum Sensorsystem für nicht intrusive Druckmessungen
EP4218829A3 (en) 2013-03-15 2023-08-16 The Board of Trustees of the Leland Stanford Junior University Activity-based probe compounds, compositions, and methods of use
JP6984909B2 (ja) 2016-12-23 2021-12-22 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 活性ベースのプローブ化合物、組成物、および使用方法
KR102669535B1 (ko) 2017-03-30 2024-05-24 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 생체 내 이미징을 위한 프로테아제 활성화 조영제

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0512334A2 (en) * 1991-05-02 1992-11-11 F. Hoffmann-La Roche Ag Methods for detecting a target nucleic acid in a sample
WO1999028500A1 (en) * 1997-11-29 1999-06-10 The Secretary Of State For Defence Fluorimetric detection system of a nucleic acid
WO1999042611A1 (en) * 1998-02-19 1999-08-26 The Secretary Of State For Defence Method for detection of target nucleic acids using pcr
WO1999066071A1 (en) * 1998-06-13 1999-12-23 Astrazeneca Uk Limited Pcr primer constructs for use in an integrated signalling system
WO2001011078A1 (en) * 1999-08-04 2001-02-15 The Secretary Of State For Defence Amplification method for detection of target nucleic acids involving fluorescence energy transfer

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1533151A (fr) * 1962-05-18 1968-07-19 Rhone Poulenc Sa Nouvel antibiotique et sa préparation
US4012284A (en) * 1962-11-16 1977-03-15 Societa' Farmaceutici Italia, S.p.A. Process of preparation of antibiotic F.I. 1762 derivatives
US3183157A (en) * 1963-02-01 1965-05-11 Upjohn Co Antibiotic nogalamycin and method of producing
US4197249A (en) * 1977-08-15 1980-04-08 American Cyanamid Company 1,4-Bis(substituted-amino)-5,8-dihydroxyanthraquinones and leuco bases thereof
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
CA1219824A (en) 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
US5208323A (en) * 1989-08-10 1993-05-04 Universite Laval Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent
HU201626B (en) 1989-09-05 1990-11-28 Magyar Kabel Muevek Device for making surface layer on work pieces moving longitudinally particularly by use of dusty integumentary material in cable industry
GB8923075D0 (en) * 1989-10-13 1989-11-29 Patterson Laurence H Anti-cancer compounds
US5849486A (en) 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US5567583A (en) * 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
DE69207580T2 (de) 1992-03-23 1996-08-08 Hoffmann La Roche Verfahren zum DNS-Nachweis
CA2170604C (en) * 1993-08-30 2007-03-13 Vikas V. Padhye Nucleic acid purification compositions and methods
JPH09503660A (ja) 1993-09-23 1997-04-15 ゼネカ・リミテッド エネルギー移動による核酸検出
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US6106777A (en) * 1994-11-09 2000-08-22 Hitachi, Ltd. DNA analyzing method and device therefor
US5858397A (en) * 1995-10-11 1999-01-12 University Of British Columbia Liposomal formulations of mitoxantrone
EP2369007B1 (en) * 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9716052D0 (en) 1996-12-06 1997-10-01 Secr Defence Reaction vessels
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
WO1998035012A2 (en) * 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
KR100386606B1 (ko) * 2001-02-03 2003-06-02 엘지전자 주식회사 Ｄｎａ 검출 방법 및 그 장치
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
KR100459394B1 (ko) * 2001-10-30 2004-12-03 엘지전자 주식회사 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법
DE60235491D1 (de) * 2001-11-28 2010-04-08 Bio Rad Laboratories Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
DE60229890D1 (de) * 2002-09-30 2008-12-24 Hoffmann La Roche Oligonukleotide zur genotypisierung des thymidylat-synthase gens
GB0223563D0 (en) * 2002-10-10 2002-11-20 Secr Defence Detection system
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
WO2005047521A2 (en) * 2003-11-10 2005-05-26 Investigen, Inc. Methods of preparing nucleic acid for detection

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0512334A2 (en) * 1991-05-02 1992-11-11 F. Hoffmann-La Roche Ag Methods for detecting a target nucleic acid in a sample
WO1999028500A1 (en) * 1997-11-29 1999-06-10 The Secretary Of State For Defence Fluorimetric detection system of a nucleic acid
WO1999042611A1 (en) * 1998-02-19 1999-08-26 The Secretary Of State For Defence Method for detection of target nucleic acids using pcr
WO1999066071A1 (en) * 1998-06-13 1999-12-23 Astrazeneca Uk Limited Pcr primer constructs for use in an integrated signalling system
WO2001011078A1 (en) * 1999-08-04 2001-02-15 The Secretary Of State For Defence Amplification method for detection of target nucleic acids involving fluorescence energy transfer

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108351349A (zh) * 2015-09-10 2018-07-31 因斯利克萨公司 用于多路定量核酸扩增的方法和系统

Also Published As

Publication number Publication date
US20040241679A1 (en) 2004-12-02
DE60204874T2 (de) 2006-04-27
PT1390542E (pt) 2005-11-30
EP1390542A2 (en) 2004-02-25
AU2002311414B2 (en) 2006-11-09
US7700275B2 (en) 2010-04-20
US20100227326A1 (en) 2010-09-09
WO2002097132A2 (en) 2002-12-05
CN1527885A (zh) 2004-09-08
HK1068651A1 (en) 2005-04-29
GB0112868D0 (en) 2001-07-18
CA2448211C (en) 2010-12-07
CA2448211A1 (en) 2002-12-05
ATE298804T1 (de) 2005-07-15
ES2240753T3 (es) 2005-10-16
DE60204874D1 (de) 2005-08-04
KR20040024556A (ko) 2004-03-20
JP2004532044A (ja) 2004-10-21
WO2002097132A3 (en) 2003-09-12
DK1390542T3 (da) 2005-08-08
EP1390542B1 (en) 2005-06-29

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