CN1167811C - 酶原性核酸的检测方法,以及相关分子和试剂盒 - Google Patents

酶原性核酸的检测方法,以及相关分子和试剂盒 Download PDF

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CN1167811C
CN1167811C CNB998111147A CN99811114A CN1167811C CN 1167811 C CN1167811 C CN 1167811C CN B998111147 A CNB998111147 A CN B998111147A CN 99811114 A CN99811114 A CN 99811114A CN 1167811 C CN1167811 C CN 1167811C
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nucleic acid
target
primer
sample
dna
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CN1319142A (zh
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A���е�
A·托德
C·J·富尔里
M·凯恩斯
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Johnson and Johnson Research Pty Ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Physics & Mathematics (AREA)
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  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB998111147A 1998-03-05 1999-02-22 酶原性核酸的检测方法,以及相关分子和试剂盒 Expired - Fee Related CN1167811C (zh)

Applications Claiming Priority (2)

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US7689998P 1998-03-05 1998-03-05
US60/076,899 1998-03-05

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CN1319142A CN1319142A (zh) 2001-10-24
CN1167811C true CN1167811C (zh) 2004-09-22

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US (3) US6140055A (enExample)
EP (1) EP1062367B1 (enExample)
JP (1) JP4318856B2 (enExample)
KR (1) KR100748863B1 (enExample)
CN (1) CN1167811C (enExample)
AT (1) ATE414169T1 (enExample)
AU (1) AU768627B2 (enExample)
BR (1) BR9908567A (enExample)
CA (1) CA2321948A1 (enExample)
DE (1) DE69939896D1 (enExample)
IL (2) IL138126A0 (enExample)
WO (1) WO1999045146A1 (enExample)
ZA (1) ZA991772B (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103013953A (zh) * 2012-10-19 2013-04-03 中国科学院成都生物研究所 一种剪切dna的dna酶

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
CN1167811C (zh) 1998-03-05 2004-09-22 约翰逊及约翰逊研究有限公司 酶原性核酸的检测方法,以及相关分子和试剂盒
DE19811618C1 (de) * 1998-03-17 2000-08-31 Andreas Jenne Ribozym codierende DNA und ein Oligonucleotidsubstrat enthaltende Zusammensetzung und Verfahren zur Messung von Transkriptionsraten
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
AU2001268481A1 (en) * 2000-06-15 2001-12-24 Board Of Regents, The University Of Texas System Regulatable, catalytically active nucleic acids
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
AU2002355571A1 (en) * 2001-08-09 2003-02-24 Archemix Corporation Nucleic acid sensor molecules and methods of using same
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
ES2524048T3 (es) 2003-03-31 2014-12-03 F. Hoffmann-La Roche Ag Composiciones y métodos para la detección de ciertos flavivirus, incluidos los miembros del serogrupo del virus de la encefalitis japonesa
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
JP2007534308A (ja) * 2003-11-19 2007-11-29 アレロジック・バイオサイエンシズ・コーポレーション 複数のフルオロフォアで標識したオリゴヌクレオチド
US20050250134A1 (en) * 2004-03-02 2005-11-10 Dawei Sheng Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20060211018A1 (en) * 2005-02-08 2006-09-21 Applera Corporation Nucleozymes and methods of use
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
JP2008539730A (ja) * 2005-05-02 2008-11-20 ストラタジーン カリフォルニア オリゴヌクレオチドのプローブ／プライマー組成物、及びポリヌクレオチドの検出方法
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
DK1948822T3 (da) * 2005-10-07 2011-10-17 Johnson & Johnson Res Pty Ltd Multikomponent-nukleinsyreenzymer og fremgangsmåder til deres anvendelse
WO2007056312A2 (en) * 2005-11-07 2007-05-18 Clontech Laboratories, Inc. Universal reporter tag for nucleozyme assays
EP1960554B1 (en) * 2005-12-07 2011-06-08 Tibotec Pharmaceuticals Methods, plasmid vectors and primers for assessing hiv viral fitness
WO2008001376A2 (en) * 2006-06-28 2008-01-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem Detection of analytes in a medium
DE102006035600B3 (de) * 2006-07-27 2007-11-08 Epigenomics Ag Verfahren zum Nachweis eines Methylierungsmusters
EP1882746A3 (en) * 2006-07-27 2008-03-19 Epigenomics AG Method for detecting a methylation pattern
BRPI0718137B1 (pt) 2006-10-06 2016-05-10 Johnson & Johnson Res Pty Ltd comutadores moleculares e métodos para seu uso
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
CN101802216B (zh) * 2007-04-05 2014-12-10 斯比戴克斯私人有限公司 核酸酶和复合体及其使用方法
EP2071035A1 (en) 2007-12-13 2009-06-17 Epigenomics AG Facilitator and method for amplification
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
CN101907573B (zh) * 2010-08-17 2012-08-22 复旦大学 一种高灵敏度的集成cmos光学生物芯片
US9051606B2 (en) 2010-09-10 2015-06-09 Qiagen Gaithersburg, Inc. Methods and compositions for nucleic acid detection
ES2617599T3 (es) 2011-04-15 2017-06-19 Becton, Dickinson And Company Termociclador microfluídico de exploración en tiempo real y métodos para termociclado sincronizado y detección óptica de exploración
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
WO2015033650A1 (ja) * 2013-09-05 2015-03-12 Ｎｅｃソリューションイノベータ株式会社 サンプルの製造方法およびターゲットの分析方法

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4000159A (en) * 1974-06-28 1976-12-28 Phillips Petroleum Company Preparation of n,n-disubstituted thioamides
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
JP3276955B2 (ja) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
NZ241310A (en) * 1991-01-17 1995-03-28 Gen Hospital Corp Trans-splicing ribozymes
GB9115909D0 (en) * 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
WO1994013833A1 (en) * 1992-12-04 1994-06-23 Innovir Laboratories, Inc. Ribozyme amplified diagnostics
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
KR100218726B1 (ko) * 1992-12-30 1999-09-01 김영환 고집적 반도체 소자의 접속장치 및 그 제조방법
IL105894A (en) * 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
IL111857A (en) * 1994-12-02 1999-09-22 Intelligene Ltd Detection of nucleic acid sequences
US5807718A (en) * 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
MX9706501A (es) * 1995-02-27 1998-02-28 Intelligene Ltd Deteccion de biomoleculas.
AUPN245295A0 (en) * 1995-04-13 1995-05-11 Johnson & Johnson Research Pty. Limited Assay for genetic abnormalities
JPH11506607A (ja) * 1995-06-07 1999-06-15 メルク エンド カンパニー インコーポレーテッド Ｄｎａポリメラーゼ伸長アッセイ
BRPI9809433B1 (pt) * 1997-04-29 2015-09-08 Scripps Research Inst moléculas de dna enzimáticas
DE19805593A1 (de) * 1998-02-12 1999-08-19 Voith Sulzer Papiertech Patent Eindicker
CN1167811C (zh) 1998-03-05 2004-09-22 约翰逊及约翰逊研究有限公司 酶原性核酸的检测方法,以及相关分子和试剂盒

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103013953A (zh) * 2012-10-19 2013-04-03 中国科学院成都生物研究所 一种剪切dna的dna酶
CN103013953B (zh) * 2012-10-19 2014-09-17 中国科学院成都生物研究所 一种剪切dna的dna酶

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DE69939896D1 (de) 2008-12-24
EP1062367B1 (en) 2008-11-12
ATE414169T1 (de) 2008-11-15
US6201113B1 (en) 2001-03-13
ZA991772B (en) 2000-10-12
EP1062367A4 (en) 2003-07-30
KR20010041591A (ko) 2001-05-25
IL164182A0 (en) 2005-12-18
US20010001063A1 (en) 2001-05-10
KR100748863B1 (ko) 2007-08-13
US6365724B2 (en) 2002-04-02
BR9908567A (pt) 2000-12-12
IL138126A0 (en) 2001-10-31
AU3340999A (en) 1999-09-20
JP2002505117A (ja) 2002-02-19
AU768627B2 (en) 2003-12-18
US6140055A (en) 2000-10-31
EP1062367A1 (en) 2000-12-27
JP4318856B2 (ja) 2009-08-26
CA2321948A1 (en) 1999-09-10
WO1999045146A1 (en) 1999-09-10
CN1319142A (zh) 2001-10-24

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