ATE414169T1 - Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits - Google Patents

Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits

Info

Publication number
ATE414169T1
ATE414169T1 AT99914694T AT99914694T ATE414169T1 AT E414169 T1 ATE414169 T1 AT E414169T1 AT 99914694 T AT99914694 T AT 99914694T AT 99914694 T AT99914694 T AT 99914694T AT E414169 T1 ATE414169 T1 AT E414169T1
Authority
AT
Austria
Prior art keywords
nucleic acid
methods
target
sample
zymogens
Prior art date
Application number
AT99914694T
Other languages
English (en)
Inventor
Alison Todd
Caroline Fuery
Murray Cairns
Original Assignee
Johnson & Johnson Res Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Res Pty Ltd filed Critical Johnson & Johnson Res Pty Ltd
Application granted granted Critical
Publication of ATE414169T1 publication Critical patent/ATE414169T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99914694T 1998-03-05 1999-02-22 Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits ATE414169T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7689998P 1998-03-05 1998-03-05

Publications (1)

Publication Number Publication Date
ATE414169T1 true ATE414169T1 (de) 2008-11-15

Family

ID=22134859

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99914694T ATE414169T1 (de) 1998-03-05 1999-02-22 Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits

Country Status (13)

Country Link
US (3) US6140055A (de)
EP (1) EP1062367B1 (de)
JP (1) JP4318856B2 (de)
KR (1) KR100748863B1 (de)
CN (1) CN1167811C (de)
AT (1) ATE414169T1 (de)
AU (1) AU768627B2 (de)
BR (1) BR9908567A (de)
CA (1) CA2321948A1 (de)
DE (1) DE69939896D1 (de)
IL (2) IL164182A0 (de)
WO (1) WO1999045146A1 (de)
ZA (1) ZA991772B (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
KR100748863B1 (ko) 1998-03-05 2007-08-13 존슨 앤드 존슨 리서치 피티와이 리미티드 효소원 핵산 검출 방법, 및 관련 분자 및 키트
DE19811618C1 (de) * 1998-03-17 2000-08-31 Andreas Jenne Ribozym codierende DNA und ein Oligonucleotidsubstrat enthaltende Zusammensetzung und Verfahren zur Messung von Transkriptionsraten
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
EP1364009A2 (de) * 2000-06-15 2003-11-26 Board of Regents, The University of Texas System Regulierbare, katalytisch-aktive nukleinsäuren
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
AU2002355571A1 (en) * 2001-08-09 2003-02-24 Archemix Corporation Nucleic acid sensor molecules and methods of using same
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
MXPA05010429A (es) 2003-03-31 2005-11-04 Hoffmann La Roche Composiciones y metodos para detectar ciertos flavivirus que incluyen miembros del serogrupo del virus de la encefalitis japonesa.
EP1689764B1 (de) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Mit mehreren fluorophoren markierte oligonukleotide
WO2005085474A2 (en) * 2004-03-02 2005-09-15 Clontech Laboratories Inc. Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
WO2006086499A2 (en) * 2005-02-08 2006-08-17 Applera Corporation Nucleozymes and methods of use
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
JP2008539730A (ja) * 2005-05-02 2008-11-20 ストラタジーン カリフォルニア オリゴヌクレオチドのプローブ／プライマー組成物、及びポリヌクレオチドの検出方法
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP2385141B1 (de) 2005-10-07 2013-08-07 SpeeDx Pty Ltd Nukleinsäureenzyme aus mehreren Komponenten und Verwendungsverfahren dafür
WO2007056312A2 (en) * 2005-11-07 2007-05-18 Clontech Laboratories, Inc. Universal reporter tag for nucleozyme assays
CA2631881C (en) * 2005-12-07 2015-03-24 Tibotec Pharmaceuticals Ltd. Methods, plasmid vectors and primers for assessing hiv viral fitness
WO2008001376A2 (en) * 2006-06-28 2008-01-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem Detection of analytes in a medium
EP1882746A3 (de) * 2006-07-27 2008-03-19 Epigenomics AG Methode zum Nachweis eines Methylierungsmusters
DE102006035600B3 (de) * 2006-07-27 2007-11-08 Epigenomics Ag Verfahren zum Nachweis eines Methylierungsmusters
ES2520023T3 (es) 2006-10-06 2014-11-11 Speedx Pty Ltd Interruptores moleculares y métodos para su uso
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
ES2425066T3 (es) * 2007-04-05 2013-10-11 Speedx Pty Ltd Enzimas y complejos de ácidos nucleicos y métodos para su uso
EP2071035A1 (de) 2007-12-13 2009-06-17 Epigenomics AG Vermittler und Verstärkungsverfahren
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
CN101907573B (zh) * 2010-08-17 2012-08-22 复旦大学 一种高灵敏度的集成cmos光学生物芯片
US9051606B2 (en) 2010-09-10 2015-06-09 Qiagen Gaithersburg, Inc. Methods and compositions for nucleic acid detection
ES2769028T3 (es) 2011-04-15 2020-06-24 Becton Dickinson Co Termociclador microfluídico de barrido en tiempo real
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
CN103013953B (zh) * 2012-10-19 2014-09-17 中国科学院成都生物研究所 一种剪切dna的dna酶
US20160202154A1 (en) * 2013-09-05 2016-07-14 Nec Solution Innovators, Ltd. Method for producing sample and method for analyzing target

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4000159A (en) * 1974-06-28 1976-12-28 Phillips Petroleum Company Preparation of n,n-disubstituted thioamides
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
JP3276955B2 (ja) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
NZ314629A (en) * 1991-01-17 2000-08-25 Gen Hospital Corp Use trans-splicing ribozymes to prepare medicaments for gene therapies
GB9115909D0 (en) * 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
ATE217347T1 (de) * 1992-12-04 2002-05-15 Univ Yale Diagnose mittels signalverstärkung durch ein ribozym
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
KR100218726B1 (ko) * 1992-12-30 1999-09-01 김영환 고집적 반도체 소자의 접속장치 및 그 제조방법
IL105894A (en) * 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5807718A (en) * 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
IL111857A (en) * 1994-12-02 1999-09-22 Intelligene Ltd Detection of nucleic acid sequences
JPH11500917A (ja) * 1995-02-27 1999-01-26 インテリジェン・リミテッド 生体分子の検出
AUPN245295A0 (en) * 1995-04-13 1995-05-11 Johnson & Johnson Research Pty. Limited Assay for genetic abnormalities
DE69618957T2 (de) * 1995-06-07 2002-08-14 Merck & Co Inc Dna-polymerase verlängungs assay
CN1261920A (zh) * 1997-04-29 2000-08-02 斯克里普斯研究学院 酶促dna分子
DE19805593A1 (de) * 1998-02-12 1999-08-19 Voith Sulzer Papiertech Patent Eindicker
KR100748863B1 (ko) 1998-03-05 2007-08-13 존슨 앤드 존슨 리서치 피티와이 리미티드 효소원 핵산 검출 방법, 및 관련 분자 및 키트

Also Published As

Publication number Publication date
KR100748863B1 (ko) 2007-08-13
AU768627B2 (en) 2003-12-18
CA2321948A1 (en) 1999-09-10
US20010001063A1 (en) 2001-05-10
EP1062367A4 (de) 2003-07-30
IL138126A0 (en) 2001-10-31
US6201113B1 (en) 2001-03-13
JP4318856B2 (ja) 2009-08-26
EP1062367B1 (de) 2008-11-12
US6365724B2 (en) 2002-04-02
KR20010041591A (ko) 2001-05-25
WO1999045146A1 (en) 1999-09-10
CN1319142A (zh) 2001-10-24
DE69939896D1 (de) 2008-12-24
US6140055A (en) 2000-10-31
EP1062367A1 (de) 2000-12-27
AU3340999A (en) 1999-09-20
ZA991772B (en) 2000-10-12
JP2002505117A (ja) 2002-02-19
BR9908567A (pt) 2000-12-12
CN1167811C (zh) 2004-09-22
IL164182A0 (en) 2005-12-18

Similar Documents

Publication Publication Date Title
ATE414169T1 (de) Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits
ATE191934T1 (de) Verfahren zur isothermer amplifikation von nukleinsäure molekülen
US7642056B2 (en) Method and kit for detecting a target protein using a DNA aptamer
ES2173670T3 (es) Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz.
DK0497784T5 (da) Kvantificering af nukleinsyrer under anvendelse af polymerasekædereaktion
DE69531831D1 (de) Verfahren mit einer hohen umsatzrate für das auffinden von sequenzen oder genetischen veränderungen in nukleinsäuren
ATE545710T1 (de) Verfahren zur vervielfältigung von nukleinsäuren
DE60134030D1 (de) Verfahren zur sequenzierung von dna
FI950487A (fi) Menetelmä, reagenssi ja testipakkaus nukleiinihapposekvenssien totamiseksi ja monistamiseksi
ATE255168T1 (de) Massenspektroskopische verfahren zur sequenzierung von nukleinsäuren
CO4870792A1 (es) Compuestos y conjunto de medios de diagnostico para detectar y vigilar la progresion de cancer de prostata
RU2008123842A (ru) Способы с использованием пор
ATE457360T1 (de) Methode zur charakterisierung von nukleinsäuren
DE69834506D1 (de) Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung
DE69940903D1 (de) Diagnostische methoden basierend auf katalytischen nukleinsäuren
DE69941690D1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
DE60332468D1 (de) Analytnachweis
DE68907305D1 (de) Methode zur analyse einer spezifischen sequenz von dns oder rns, reagenzien und kits dafuer.
DE60121547D1 (de) Electrochemisches verfahren zum nachweis von nukleinsäuren
DE69610933T2 (de) Verfahren und reagenzien zur typisierung von hla-genen der klasse i
ATE465268T1 (de) Verfahren zur mikrobiologischen isomerisierung von alpha-hydroxycarbonsäuren
IL143638A0 (en) Method for detecting and/or quantifying a known function from a nucleic acid sample
SE0101934D0 (sv) New methods
WO2002044405A3 (de) Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen
ATE357536T1 (de) Oligonukleotide, verfahren und kit zur detektion von listeria monocytogenes durch nukleinsäureamplifikation und/oder - hybridisierung

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties